Preliminary investigation and detection based on loop-mediated isothermal amplification(LAMP)of phytoplasmas associated with diseases in *** L.

作     者：Yancheng Wen Shufen Zhang Junping He Dongfang Cai Jiacheng Zhu Jianping Wang Jinhua Cao Kun Hu Lei Zhao Dongguo Wang Yizi Liu Yancheng Wen;Shufen Zhang;Junping He;Dongfang Cai;Jiacheng Zhu;Jianping Wang;Jinhua Cao;Kun Hu;Lei Zhao;Dongguo Wang;Yizi Liu

作者机构：Institute of Industrial Crops of Henan Academy of Agricultural SciencesZhengzhou450002China 

出 版 物：《Oil Crop Science》 (中国油料作物学报(英文版）)

年 卷 期：2022年第7卷第4期

页      面：219-224页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：the financial support provided by project of Science and Technology Tackling Key Problems in Henan Province(Grant No.182102110430) the National Key Research and Development Program of China(Grant No.2016YFD0101300) by China Agricultural Research system(CARS-12) 

主　　题：B.napus L. Phytoplasma associated disease Pathogen Detection Loop-mediated isothermal amplification (LAMP) 

摘      要：In the last decade,some disease occurred on our experimental farms that had caused serious *** were not caused by fungi,bacteria or *** loop-mediated isothermal amplification(LAMP)technique,the detection results pointed to the possible pathogen as *** investigation results implied that phytoplasmas could cause more than 13 kinds of symptoms in almost all parts of plants in *** L.,including witches’broom,multi-stems,aggregate main inflorescences,and flat *** incidences of these phytoplasma-associated diseases in our experimental farms rose from 1.61%in 2010 to 6.00%in *** phytoplasma infected plants died without any growing *** studies would be helpful for detecting phytoplasmas diseases,selecting disease resistant germplasm and improving varieties with disease resistances in *** L.