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Biomedical and Environmental Sciences 2006年第5期19卷 340-345页

作者： WEI HE ZHEN-yu JI cheng-yu huang Department of Nutrition and Food Hygiene Huaxi School of Public Health Sichuan University Chengdu 610041 Sichuan China Henan Institute of Medical Sciences Zhengzhou 450052 Henan China

Objective To study the mechanism of lactose intolerance （LI） by cloning the mouse lactase cDNA and recombining a vector. Methods Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse （δ）. Crene-specific primers were designed and synthesized according to the cDNA sequences of lactase-phlorizin hydrolase （LPH） in human, rat, and rabbit. A coding sequence （CDS） fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics. Results The cDNA from the BALB/c mouse with 912 bp encoding 303 amino acid residues. Analysis of the deduced amino acid sequence using bioinforrnatics revealed that this cDNA shared extensive sequence homology with human LPH containing a conserved glycosyl hydrolase family 1 motif important for regulating lactase intolerance. Conclusion BALB/c mouse LPH cDNA （GenBank accession No： AY751548） provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice.

关键词： BALB/c mice LPH cDNA pNEB 193 Cloning Sequence

Attributable Causes of Cancer in China:Fruit and Vegetable

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Chinese Journal of Cancer Research 2011年第3期23卷 171-176页

作者： Hui-juan Xiao Hao Liang Jian-bing Wang cheng-yu huang Wen-qiang Wei Mathieu Boniol You-lin Qiao Paolo Boffetta Department of Nutrition and Food Hygiene West China School of Public Health Sichuan University Chengdu 610041 China Departments of Cancer Epidemiolo gy Cancer Institute Chinese Academy of Medical Sciences Beijing 100021 China Department of Epidemiology and Statistics Institute of Basic Medical Sciences Chinese Academy of Medical Sciences ＆School of Basic Medicine Peking Union Medical College Beijing 100005 China International Prevention Research Institute Lyon 69008 France The Tisch Cancer Institute Mount Sinai School of Medicine New York NY 10029 USA Nutrition Department Tianjin Third Central Hospital Tianjin 300170 China

Objective：To provide an evidence-based and consistent assessment of the burden of cancer attributable to inadequate fruit and vegetable intake in China in 2005.Methods：The proportions of cancers attributable to low consumption of vegetable and fruit were calculated separately to estimate the burden of related cancers for the year 2005 in China.Data on the prevalence of exposure were derived from a Chinese nutrition and health survey.Data on relative risks were mainly derived from meta-analysis.Attributable fractions were calculated based on the counterfactual scenario which was a shift in the exposure distribution.Results：The total cancer burden attributable to inadequate consumption of fruit was up to 233,000 deaths （13.0% of all cancers） and 300,000 cases （11.6% of all cancers） in 2005.Increasing consumption of vegetable to the highest quintile could avoid total cancer deaths and cases by 3.6% （64,000 persons） and 3.4% （88,000 persons）.The contributions to cancer burden were higher in rural areas than in urban areas.They have greater influence on men than on women.The largest proportions of cancer burden attributable to low fruit and vegetable intake were for oral and pharyngeal cancers.Conclusion：This study showed that inadequate intake of fruit and vegetable makes a significant contribution to the cancer burden.Increasing consumption of fruit and vegetable could prevent many cancer deaths and save many lives.Promoting the consumption of fruit and vegetable is an important component in diet-based strategies for preventing cancer.

关键词： Fruit Vegetable Cancer Population attributable fraction China

Catch organism assemblages along artificial reefs area and adjacent waters in Haizhou Bay

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Acta Oceanologica Sinica 2024年第2期43卷 34-42页

作者： Shike Gao Bin Xie chengyu huang Xiao Zhang Shuo Zhang Wenwen yu College of Marine Living Resource Sciences and Management Shanghai Ocean UniversityShanghai 201306China Jiangsu Research Institute of Marine Fisheries Nantong 226007China Third Institute of Oceanography Ministry of Natural ResourcesXiamen361005China Joint Laboratory for Monitoring and Conservation of Aquatic Living Resources In the Yangtze Estuary Shanghai 200000China

To better understand the community patterns mediated by connectivity in artificial reefs of coastal areas, it is necessary to understand the distribution and coexistence of organisms with artificial reefs area and adjacent waters. This study was conducted to examine main catches assemblages collected by trawls in Haizhou Bay,which included five habitats: the artificial reef area(AR), aquaculture area(AA), natural area(NA), estuary area(EA) and comprehensive effect area(CEA). The result shows that the total abundances of species in the five habitats were highly different(univariate PERMANOVA: P = 0.001, n = 24), but some species were also unique in their habitat(e.g. Scapharca subcrenata and Glossaulax didyma in AA). The body size distribution of specific species between habitats are different. For Collichthys lucidus, their body size in AR(14.63 cm ± 1.64 cm) and EA(14.3 cm ± 0.85 cm) is higher than that in NA(10.65 cm ± 1.64 cm), CEA(11.28 cm ± 1.85 cm) and AA(12.1 cm ±0.43 cm), which indicates the potential connection from AR to EA mediated by their adult population. We concluded that artificial reefs in AR can be considered key components that have the ability to support species assemblages in adjacent habitats. This study has implications for the conservation and monitoring of species assemblages in coastal areas in terms of that artificial reefs can be applied in different stages of habitat protection implementation and in different combinations of scenarios.

关键词： assemblage artificial reefs adjacent water Haizhou Bay

Regulation on polymerization degree and surface feature in graphitic carbon nitride towards efficient photocatalytic H2 evolution under visible-light irradiation

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Journal of Materials Science & Technology 2022年第3期98卷 160-168页

作者： cheng cheng Chung-Li Dong Jinwen Shi Liuhao Mao yu-cheng huang Xing Kang Shichao Zong Shaohua Shen International Research Center for Renewable Energy(IRCRE) State Key Laboratory of Multiphase Flow in Power Engineering(MFPE)Xi'an Jiaotong University(XJTU)28 West Xianning RoadXi’an 710049China Department of Physics Tamkang University151 Yingzhuan RoadTamsui 25137TaiwanChina

A kind of graphitic carbon nitride(TSC-550) with high polymerization degree and improved surface property was prepared by a new precursor of thiosemicarbazide. The sulfur motif and high nitrogen content in thiosemicarbazide promoted the polymerization of thiosemicarbazide to form graphitic carbon nitride framework with high degree of polymerization, which significantly influenced the electronic structure and surface chemical properties. TSC-550 possessed a narrow bandgap of 2.19 eV that facilitated the utilization of visible light, and possessed a less positive charge, acidic surface that resulted in enhanced hydrogen adsorption ability in water solution, which promoted the H;evolution kinetics. In addition, the extended π-conjugated electronic system promoted the separation and migration of photogenerated charge carries in plane of TSC-550 framework, as well as the increasing interlayer C–N interactions in TSC-550 created conductive paths across the layers to tunnel interlayers for rapid electron transportation. As a result, TSC-550 nanosheets showed excellent photocatalytic H;production activity,the AQY achieved 36.4% at 425 nm.

关键词： Degree of polymerization Graphitic carbon nitride Photocatalysis Solar energy Hydrogen

Application of Structural Equation Model to Evaluate the Perception of Service Quality of Medical Staffs of infectious Disease Department in Chinese Hospitals

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国际感染病学（电子版） 2014年第4期3卷 179-186页

作者： Min Li Ren-tian Cai cheng-yu huang Nanjing University of Aeronautics and Astronautics Shanghai Public Health Clinical Center Fudan University Chongqing Infectious Disease Medical Center

Objective To enhance the quality of medical service for Chinese patients through research of service quality from Chinese medical personnel. Methods Serv Qual scale was used for infection medical staffs randomly by sampling questionnaire in Beijing, Shanghai, chengdu, Chongqing, Guangzhou and Nanning. The data collected were entered and analyzed using SPSS 20.0. Statistical methods included frequency, factor analysis, reliability analysis, correlation analysis, independent samples t test, one-way analyses of variance, simultaneous regression analysis and structural equation model analysis. Results The Kaiser-Meyer-Olkin value for the factor analysis of the scale was 0.970. The Cronbach's α for the reliability analysis was 0.975. The Pearson correlation coefficients were 0.624-0.874 and statistically significant. Undergraduates felt good, Ph D students felt bad; the doctors felt bad; managers felt good. Standard 5 dimensions of the regression coefficients were positive, including empathy(β = 0.288), reliability(β = 0.241) impacting on perceived service quality mostly. The control ability and stability of the standard error of perceived service quality directly effected value were 0.646 and 0.382, respectively. Conclusions Medical staffs of infectious disease department have poor perception of service quality. Hospitals should improve awareness and of clinicians and deepen the reform of the medical care system.

关键词： Structural equation models Infectious disease department Perception of service quality

Cosmological interpretation for the stochastic signal in pulsar timing arrays

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Science China(Physics,Mechanics & Astronomy) 2024年第4期67卷 43-50页

作者： yu-Mei Wu Zu-cheng Chen Qing-Guo huang School of Fundamental Physics and Mathematical Sciences Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China Department of Astronomy Beijing Normal UniversityBeijing 100875China Advanced Institute of Natural Sciences Beijing Normal UniversityZhuhai 519087China Department of Physics and Synergistic Innovation Center for Quantum Effects and Applications Hunan Normal UniversityChangsha 410081China CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China

The pulsar timing array(PTA)collaborations have recently reported compelling evidence for a stochastic signal consistent with a gravitational-wave background.In this paper,we combine the latest data sets from NANOGrav,PPTA,and EPTA to explore cosmological interpretations for the PTA signal from first-order phase transitions,domain walls,and cosmic strings,respectively.We find that the domain wall model is strongly disfavored with the Bayes factors compared with the first-order phase transitions and cosmic strings being 1/90 and 1/189,respectively,breaking the degeneracy among these models in individual data set.We also find that:(1)a strong phase transition at temperatures below the electroweak scale is favored,and the bubble collisions make the dominant contribution to the energy density spectrum;(2)a small reconnection probability p<1.55×10^(-1)allowed by strings in(super)string theory is favored at the 95%confidence level,and ground-based detectors can further constrain the parameter space.

关键词： puslar timing arrays gravitational-wave background first-order phase transitions domain walls cosmic strings

Facile synthesis of high-brightness green-emitting carbon dots with narrow bandwidth towards backlight display

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Chinese Chemical Letters 2024年第5期35卷 277-282页

作者： Rui cheng Tingting Zhang Xin huang Jian yu State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing 210009China

High-performance carbon dots(CDs)allowing the application in high-end display devices are highly desirable and usually limited by the absence of simple and easy synthesis methods.In this work,we exploited an easy-to-implement strategy for the one-step synthesis of green-emitting CDs(G-CDs)with superb optical properties.The G-CDs were synthesized using m-phenylenediamine(m-PD)as a single precursor,and the reaction reacted at 180℃for 12 h The resultant G-CDs exhibit high-purity and excitationindependent green fluorescence with the photoluminescence(PL)peak located at 516 nm,full width at half maximum(FWHM)of 46 nm,and PL quantum yield(QY)of∼80%under the 470nm excitation light.The G-CDs and corresponding composite film prepared with polyvinyl butyral(G-CDs@PVB)exhibit good PL stability after undergoing long-time storage for one year and 360 h exposure under 460nm blue light.The G-CDs@PVB film was used as color-conversion materials in green-emitting light-emitting diode(LED)application,exhibiting a Commission internationale de l’Eclairage(CIE)chromaticity coordinate of(0.21,0.44).The film was also used in CD-based liquid crystal display(CD-LCD)application,achieving a color gamut value of 85%.This work will offer a working basis for the synthesis of high-performance CDs as well as their application in displays.

关键词： Carbon dots One-step synthesis Narrow bandwidth High quantum yield Display

Electron-Deficient Zn-N_(6) Configuration Enabling Polymeric Carbon Nitride for Visible-Light Photocatalytic Overall Water Splitting

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Nano-Micro Letters 2022年第12期14卷 602-616页

作者： Daming Zhao Yiqing Wang Chung-Li Dong Fanqi Meng yu-cheng huang Qinghua Zhang Lin Gu Lan Liu Shaohua Shen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power EngineeringXi’an Jiaotong UniversityXi’an 710049People’s Republic of China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022People’s Republic of China Department of Physics Tamkang UniversityNew Taipei City 25137TaiwanPeople’s Republic of China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190People’s Republic of China

Despite of suitable band structures for harvesting solar light and driving water redox reactions,polymeric carbon nitride(PCN)has suffered from poor charge transfer ability and sluggish surface reaction kinetics,which limit its photocatalytic activity for water splitting.Herein,atomically dispersed Zn-coordinated three-dimensional(3D)sponge-like PCN(Zn-PCN)is synthesized through a novel intermediate coordination strategy.Advanced characterizations and theoretical calculations well evidence that Zn single atoms are coordinated and stabilized on PCN in the form of Zn-N_(6) configura-tion featured with an electron-deficient state.Such an electronic configuration has been demonstrated contributive to promoted electron excitation,accelerated charge separation and transfer as well as reduced water redox barriers.Further benefited from the abundant surface active sites derived from the 3D porous structure,Zn-PCN realizes visible-light photocatalysis for overall water splitting with H_(2) and O_(2) simultaneously evolved at a stoichiometric ratio of 2:1.This work brings new insights into the design of novel single-atom photocatalysts by deepening the understanding of electronic configurations and reactive sites favorable to excellent photocatalysis for water splitting and related solar energy conversion reactions.

关键词： Zn single atoms Polymeric carbon nitride Overall water splitting Photocatalysis

Effect of Frequency on Degradation in BOPP Films Under Repetitively Pulsed Voltage

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CSEE Journal of Power and Energy Systems 2024年第3期10卷 1280-1290页

作者： Chuansheng Zhang yu Feng Fei Kong Bangdou huang cheng Zhang Tao Shao Beijing International S&T Cooperation Base for Plasma Science and Energy Conversion Institute of Electrical EngineeringChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Operating conditions of film capacitors are complex,and the problem of film insulation failure caused by repetitively pulsed voltage is becoming ever serious.Degradation of the film under repetitively pulsed voltage cannot be accurately evaluated by the average breakdown electric field.In this paper,the effects of pulsed electric field and pulse repetition frequency on the breakdown in biaxially oriented polypropylene(BOPP)films are investigated.Three phases of BOPP degradation are proposed based on the voltage amplitude,i.e.,maintenance(M),decline(D),and near-zero(N).Evolution of the BOPP film from degradation to breakdown at different frequencies is presented.Meanwhile,transition of discharge mode and elemental composition of the film are analyzed.Experimental results show continuous heat generation under repetitive microsecond pulses is the dominant factor for degradation of BOPP film.The number of applied pulses and the repetitive stressing time decrease exponentially with increase of frequency.This research can be contributed to the safe and reliable operation of capacitors.

关键词： Aging breakdown film capacitor polypropylene repetitive pulse

Single-atom alloys prepared by two-step thermal evaporation

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Nano Research 2024年第4期17卷 2808-2813页

作者： Honglin Wang Jing Li Meirong huang Jizhe Cui Zhiying cheng Rong yu Hongwei Zhu State Key Lab of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China State Key Lab of Solid Waste Reuse for Building Materials Beijing Building Materials Academy of Science ResearchBeijing 100041China

Single-atom alloys(SAAs)have gained significant attention due to their remarkable atomic utilization efficiency,interactions between single atoms(SAs)and metal supports,and free-atom-like electronic structure of dopant elements.In this work,we observed the formation of SAs in pre-deposited metal particles by a two-step thermal evaporation technique,thereby establishing the first instance of discovering SAAs by thermal evaporation.The discovery of SAAs by thermal evaporation extends the range of SAAs preparation methods to include this traditional synthetic technique,which offers convenience,cost-efficiency,and universality.The formation mechanism of SAAs prepared using this technique was elucidated by density functional theory calculations.It was demonstrated that thermal evaporation can be utilized to prepare SAAs with multiple SAs,further highlighting its universal applicability.

关键词： single-atom alloys thermal evaporation density functional theory