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Amplification and function analysis of N6-adenine-specific DNA methyltransferase gene in Nilaparvata lugens

Journal of Integrative Agriculture 2016年第3期15卷 591-599页

作者： ZHANG Jiao XING Yan-ru HOU Bo-feng yuan Zhu-ting LI Yao JIE Wen-cai SUN Yang LI Fei Department of Entomology College of Plant Protection Nanjing Agricultural UniversityKey Laboratory of Monitoring and Management of Plant Diseases and Insects Ministry of Agriculture key Lab of Agricultural Entomology Ministry of AgricultureInstitute of Insect Sciences Zhejiang University

Methylation of the N6 position of adenine, termed N6-methyladenine, protects DNA from restriction endonucleases via the host-specific restriction-modification system. N6-methyladenine was discovered and has been well studied in bacteria. N6-adenine-specific DNA methyltransferase（N6AMT） is the main enzyme catalyzing the methylation of the adenine base and knowledge of this enzyme was mainly derived from work in prokaryotic models. However, large-scale gene discovery at the genome level in many model organisms indicated that the N6AMT gene also exists in eukaryotes, such as humans, mice, fruit flies and plants. Here, we cloned a N6AMT gene from Nilaparvata lugens（Nlu-N6AMT） and amplified its fulllength transcript. Then, we carried out a systematic investigation of N6AMT in 33 publically available insect genomes, indicating that all studied insects had N6AMT. Genomic structure analysis showed that insect N6AMT has short introns compared with the mammalian homologs. Domain and phylogenetic analysis indicated that insect N6AMT had a conserved N6-adenine Mlase domain that is specific to catalyze the adenine methylation. Nlu-N6AMT was highly expressed in the adult female. We knocked down Nlu-N6AMT by feeding ds RNA from the second instar nymph to adult female, inducing retard development of adult female. In all, we provide the first genome-wide analysis of N6AMT in insects and presented the experimental evidence that N6AMT might have important functions in reproductive development and ovary maturation.

关键词： N6-adenine-specific DNA methyltransferase(N6AMT) Nilaparvata lugens expression RNA interference gene structure

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4℃冷藏保存血小板输注对大鼠失血性休克/复苏致急性肾损伤的影响研究

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临床输血与检验 2024年第4期26卷 470-475页

作者：谯铭铭贡觉顿珠陈元锋于媛叶辉庄云龙山东省血液中心质量管理科山东济南250014

目的研究4℃冷藏保存血小板(cold storage platelets,cSP)对大鼠失血性休克/复苏(HS/R)导致急性肾损伤的影响。方法健康雄性SD大鼠40只,随机选取20只用以制备血小板制剂,剩余大鼠随机分为4组:假手术组(空白组)、HS/R模型组(模型组)、cS... 详细信息

目的研究4℃冷藏保存血小板(cold storage platelets,cSP)对大鼠失血性休克/复苏(HS/R)导致急性肾损伤的影响。方法健康雄性SD大鼠40只,随机选取20只用以制备血小板制剂,剩余大鼠随机分为4组:假手术组(空白组)、HS/R模型组(模型组)、cSP输注组、22℃±2℃振荡保存血小板输注组(RTP输注组)。容量复苏后24 h处死动物,采集血和肾组织标本。检测各组血清中血肌酐(Scr)、尿素氮(BUN)、血清胱抑素c(cysc)及肾组织中白细胞介素1β(IL-1β)、白细胞介素18(IL-18)含量。Western Blot法检测各组大鼠肾组织中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、肾损伤因分子1(kIM-1)表达量,HE染色观察肾组织病理学改变。结果①四组大鼠基线状态体质量、MAP、Scr、BUN等指标无明显差异(P>0.05)。②复苏后24 h,模型组大鼠Scr(Pcysc(PcSP输注组BUN(Pcr(P=0.037)、cysc(P=0.020)水平低于RTP输注组。③与模型组比较,cSP输注组和RTP输注组血清IL-1β和IL-18水平均显著降低。较于RTP输注组,cSP输注组血清IL-1β水平有所降低(P=0.041)。④与模型组相比,cSP输注组NGAL蛋白表达明显下降(PcSP输注组和RTP输注组kIM-1蛋白表达均显著下降(PcSP输注可减轻肾细胞变性、坏死及炎性细胞浸润程度。结论较于RTP,cSP输注可减轻SD大鼠HS/R急性肾损伤。

关键词： 4℃冷藏保存血小板失血性休克急性肾损伤

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Associations of HDL-c/LDL-c with myocardial infarction,all-cause mortality,haemorrhagic stroke and ischaemic stroke:a longitudinal study based on 384093 participants from the Uk Biobank

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Stroke & Vascular Neurology 2023年第2期8卷 119-126页

作者： Shiqi yuan Xiaxuan Huang Wen Ma Rui Yang Fengshuo Xu Didi Han Tao Huang MIn Peng Anding Xu Jun Lyu Department of Neurology Jinan University First Affiliated HospitalGuangzhouGuangdongChina School of Public Health Xi'an Jiaotong UniversityXi'anShaanxiChina Department of clinical Research Jinan University First Affiliated HospitalGuangzhouGuangdongChina Guangdong Provincial key Laboratory of Traditional chinese Medicine Informatization GuangzhouGuangdongChina

Objective To explore the correlations of high-density lipoprotein cholesterol(HDL-c)/low-density lipoprotein cholesterol(LDL-c)with myocardial infarction(MI),all-cause mortality,haemorrhagic stroke and ischaemic stroke,as well as the joint association of genetic susceptibility and HDL-c/LDL-c with the MI *** and results This study selected 384093 participants from the Uk Biobank(UkB)***,restricted cubic splines indicated non-linear associations of HDL-c/LDL-c with MI,ischaemic stroke and all-cause ***,a cox proportional-hazards model indicated that compared with HDL-c/LDL-c=0.4-0.6,HDL-c/LDL-c0.6 were correlated with all-cause mortality(HR=0.97 for HDL-c/LDL-ccI=0.939 to 0.999,pc/LDL-c>0.6,95%cI=1.16 to 1.26,pc/LDL-ccorrelated with a higher MI risk(HR=1.36,95%cI=1.28 to 1.44,pchaemic stroke(HR=1.12,95%cI=1.02 to 1.22,pc/LDL-c>0.6 was associated with higher risk haemorrhagic stroke risk after full multivariable adjustment(HR=1.25,95%cI=1.03 to 1.52,pcalculating the coronary heart disease Genetic Risk Score(cHD-GRS)of each participant,the cox proportional-hazards model indicated that compared with low cHD-GRS and HDL-c/LDL-c=0.4-0.6,participants with a combination of high cHD-GRS and HDL-c/LDL-cciated with the highest MI risk(HR=2.45,95%cI=2.15 to 2.8,pcipants with HDL-c/LDL-ccorrelated with a higher MI risk regardless of whether they had a high,intermediate or low *** In UkB participants,HDL-c/LDL-c ratio of 0.4-0.6 was correlated with lower MI risk,all-cause mortality,haemorrhagic stroke and ischaemic *** with HDL-c/LDL-ccorrelated with a higher MI risk regardless of whether they had a high,intermediate or low *** clinical significance and impact of HDL-c/LDL-c need to be further

关键词： infarction haemorrhagic restricted

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Echinacoside Protects Against MPP+-Induced Neuronal Apoptosis via ROS/ATF3/cHOP Pathway Regulation

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Neuroscience Bulletin 2016年第4期32卷 349-362页

作者： Qing Zhao Xiaoyan Yang Dingfang cai Ling Ye Yuqing Hou Lijun Zhang Jiwei cheng yuan Shen kaizhe Wang Yu Bai Department of Neurology Putuo HospitalShanghai University of Traditional Chinese Medicine Department of Emergency Internal Medicine Putuo HospitalShanghai University of Traditional Chinese Medicine Laboratory of Neurology Institute of Integrative MedicineZhongshan Hospital Fudan University Department of Integrative Medicine Zhongshan HospitalFudan University center for Translational Neurodegeneration and Regenerative Therapy Shanghai Tenth People’s Hospital Affiliated with Tongji University School of Medicine Department of Immunology Tongji University School of Medicine

Echinacoside （EcH） is protective in a mouse model of Parkinson＇ s disease （PD） induced by 1-methyl-4- phenylpyridinium ion （MPP＋）. To investigate the mechanisms involved, SH-SYSY neuroblastoma ceils were treated with MPP＋ or a combination of MPP＋ and EcH, and the expression of ATF3 （activating transcription factor 3）, cHOP （c/EBP-homologous protein）, ScNA （synuclein alpha）, and GDNF （glial cell line-derived neurotrophic factor） was assessed. The results showed that EcH significantly improved cell survival by inhibiting the generation of MPP＋-induced reactive oxygen species （ROS）. In addition, EcH suppressed the ROS and MPP＋- induced expression of apoptotic genes （ATF3, cHOP, and ScNA）. EcH markedly decreased the MPP＋-induced cas- pase-3 activity in a dose-dependent manner. ATF3- knockdown also decreased the cHOP and cleaved caspase- 3 levels and inhibited the apoptosis induced by MPP＋. Interestingly, EcH partially restored the GDNF expression that was down-regulated by MPP＋. EcH also improved dopaminergic neuron survival during MPP＋ treatment and protected these neurons against the apoptosis induced by MPTP. Taken together, these data suggest that the ROS/ ATF3/cHOP pathway plays a critical role in mechanisms by which EcH protects against MPP＋-induced apoptosis in PD.

关键词： Echinacoside Parkinson's disease 1-Methyl-4-phenylpyridinium ion. Reactive oxygen species ATF3 cHOP

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Ti掺杂苯基与乙基桥连的有序介孔有机硅的制备及其催化环己烯氧化反应

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分子催化 2015年第2期29卷 135-142页

作者：袁佩黄依斌袁霞罗和安湘潭大学化工学院湖南湘潭411105

分别采用1,4-(双乙氧基硅烷)苯(1,4-BTEB)和1,2-三乙氧基硅基乙烷(1,2-BTESE)作为有机硅源,正硅酸乙酯(TEOS)为无机硅源,钛酸四丁酯(TBOT)为钛源,以Pluronic EO20PO70EO20(P123)为模板剂在酸性环境下水热合成制备了Ti掺杂的有机-无机有... 详细信息

分别采用1,4-(双乙氧基硅烷)苯(1,4-BTEB)和1,2-三乙氧基硅基乙烷(1,2-BTESE)作为有机硅源,正硅酸乙酯(TEOS)为无机硅源,钛酸四丁酯(TBOT)为钛源,以Pluronic EO20PO70EO20(P123)为模板剂在酸性环境下水热合成制备了Ti掺杂的有机-无机有序介孔有机硅材料Ti-SBA-15-ben和Ti-SBA-15-et.同时,在合成过程中加入H2O2作为配合剂调节钛源水解速度,制备得到Ti-SBA-15-ben-H和Ti-SBA-15-et-H.采用FT-IR、DR UV-Vis、N2物理吸附、XRD、TG-DSc、TEM等方法对制备的样品进行了表征.结果表明:合成过程中加入H2O2制备的苯基桥连有机硅杂化材料具有较好的疏水性能,其骨架中活性4价位钛含量高,结构有序性最好.在以叔丁基过氧化氢(TBHP)为氧源的环己烯氧化反应中对制备的催化材料进行了对比评价,结果表明:Ti-SBA-15-ben-H表现出最高的催化活性,其催化的反应以环氧化产物为主,环己烯的转化率为26.9%,环氧选择性为32.8%,T i-SBA-15-et催化的反应以烯丙位氧化产物2-环己烯-1-酮为主,环己烯的转化率为8.5%,2-环己烯-1-酮选择性为41.2%.

关键词：钛有序介孔有机硅骨架修饰 H2O2 环己烯氧化

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Oxyresveratrol suppresses proliferation and metastasis of hepatocellular carcinoma by inhibiting autophagy in vitro and in vivo

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中国药理学与毒理学杂志 2019年第9期33卷 695-696页

作者： SONG Ying-qi WU Xi-cheng LI Rong JI Zhen-ni MA Xiu-ying XIONG Wen-bi ZHANG yuan-yuan ZHOU Li-ming Department of Pharmacology West China School of Basic Medical Sciences and Forensic MedicineSichuan UniversityChengdu 610041China Basic Medicine 2015 Undergraduate Students West China School of Basic Medical Sciences and Forensic MedicineSichuan UniversityChengdu 610041China State key Lab of Biotherapy and cancer center West China HospitalSichuan University and Collaborative Innovation Center for BiotherapyChengdu 610041China

OBJEcTIVE Oxyresveratrol(OXY)has many biological activities including anti-inflammation,anti-oxida⁃tive stress,anti-cancer and ***,the mechanisms underlying its effects against hepatocellular carcinoma(Hcc)remain poorly *** Two Hcc cell lines,HepG2 and SMMc-7721 cells,were *** proliferation was determined by cck8 *** migration and invasion of cells were examined by wound healing and transwell *** of Hcc was detected by in vivo ***,transcriptome analysis was applied to explore the mechanisms of *** results of transcriptome analysis were validated by in vitro ***⁃more,western blot was used to measure the expression of Lc3 and p62 *** OXY significantly inhibited the proliferation,migration and invasion of Hcc cells in *** suppressed the metastasis of Hcc in Balb/c mice with attenuated side effects compared to *** profiling analysis revealed that OXY may affect autophagy related signaling pathway of HepG2 *** blotting showed that OXY significantly inhibite autophagy by downregulating Lc3 and upregulating p62 genes *** Our study demonstrated that OXY inhibits the metastasis of Hcc by inhibiting autophagy,which suggested OXY to be a candidate for Hcc metastasis.

关键词： oxyresveratrol autophagy metastasis

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Prevalence and patterns of drug-resistance mutations among HIV-1 patients infected with cRF07_Bc strains in Sichuan province,china

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Virologica Sinica 2014年第4期29卷 237-241页

作者： Ling Su Xia Zhou Dan yuan Hong Yang Dongbing Wei Guangmin Qin Shu Liang Sichuan Provincial center for Disease control and Prevention Center for AIDS/STD Control and Prevention University of kansas Medical center Department of Internal Medicine Kansas City

Little information is available on the prevalence of drug-resistance mutations in patients harboring the human immunodeficiency virus type 1(HIV-1) circulating recombinant form(cRF)07_Bc variant in Sichuan, china. This study examined 375 plasma samples from patients with HIV-1 who were infected with the cRF07_Bc strain, including 104 drug-naive participants and 271 in whom antiretroviral therapy(ART) had failed. Only one participant in the drug-naive group had a drug-resistance mutation(M46L), compared with 31.73% of those in whom ART had failed. Further analysis showed that 19.56% of strains contained mutations conferring resistance to non-nucleoside reverse transcriptase inhibitors(NNRTIs) alone, 0.74% were resistant to nucleoside reverse transcriptase inhibitors(NRTIs) alone, and 11.44% were dual-resistant to both NRTIs and NNRTIs. The most common mutation in the ART-failure group was M184V(35.88%), k103N(45.01%), Y181c(17.33%), and G190S/A(15.88%). The percentages of HIV-1 strains resistant to lamivudine, emtricitabine, efavirenz, etravirine, and nevirapine were 10.70%, 10.70%, 28.04%, 7.75%, and 26.20%, respectively. To explore site variants possibly related to drug resistance, variations in the ancestor/consensus cRF07_Bc sequences from the therapy-naive and ART-failure groups were compared, and seven mutations at six positions were identified as being significantly differently distributed between the two groups(pce data will provide information on cRF07_Bc genetic characterizations, and improve our understanding of antiretroviral susceptibility and the evolution of drug-resistance mutations. This will be valuable in developing and implementing local public-health approaches for HIV drug-resistance prevention and treatment.

关键词： HIV-1 cRF07_Bc drug resistance Sichuan china

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ZE10镁合金板拉胀复合成形与扩孔成形性能研究

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材料热处理学报 2007年第3期28卷 54-57页

作者：刘英李元元李卫暨南大学材料系广东广州510632 华南理工大学金属新材料广东省重点实验室广东广州510640

为研究ZE10镁合金的成形性能,在20～300℃条件下对ZE10镁合金板进行拉伸力学性能、锥杯及扩孔试验.结果表明:随着变形温度的升高,ZE10镁合金板的抗拉强度逐渐下降而伸长率、'拉深+胀形'复合成形性能、扩孔成形性能显著提高.200℃、250... 详细信息

为研究ZE10镁合金的成形性能,在20～300℃条件下对ZE10镁合金板进行拉伸力学性能、锥杯及扩孔试验.结果表明:随着变形温度的升高,ZE10镁合金板的抗拉强度逐渐下降而伸长率、'拉深+胀形'复合成形性能、扩孔成形性能显著提高.200℃、250℃锥杯实验时,ZE10镁合金试样可顺利拉深进入锥杯凹模底部直圆孔而不出现开裂,具有较小的锥杯值;而300℃时反而增加,说明在200℃、250℃时ZE10镁合金具有最佳的拉胀复合成形性能.150℃、200℃扩孔试验时,试样中心φ10 mm预制孔可胀大到凸模直径φ25 mm大小,成功实现翻边(翻边系数为0.37),显示ZE10镁合金具有很好的扩孔成形性能.

关键词：镁合金 '拉深+胀形'复合成形扩孔成形性能

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Remarkable rate acceleration of SmI3-mediated iodination of acetates of Baylis-Hillman adducts in ionic liquid: facile synthesis of (Z)-allyl iodides

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第3期7卷 193-197页

作者： LIU Yun-kui ZHENG Hui XU Dan-qian XU Zhen-yuan ZHANG Yong-min State key Laboratory Breeding Base of Green chemistry-Synthesis Technology Zhejiang University of Technology Hangzhou 310014 China

Stereoselective transformation of Baylis-Hillman acetates I into corresponding （Z）-allyl iodides 2 has been achieved by treatment of I with samarium triiodide in THF. Remarkable rate acceleration of samarium triiodide-mediated iodination of 1 was found when ionic liquid 1-n-butyl-3-methyl-imidazolium tetrafluroborate （[bmim]BF4） was used as reaction media in stead of THF. This novel approach proceeds readily at 50 ℃ within a few minutes to afford （Z）-allyl iodides 2 in excellent yields. A mechanism involving stereoselective iodination of the acetates of Baylis-Hillman adducts by samarium triiodide is described, in which a six-membered ring transition state played a key role in the stereoselective formation of 2.

关键词： Baylis-Hillman adduct (Z)-allyl iodide Samarium triiodide Ionic liquid

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Synthesis and Binding Properties of Novel cyclodextrin Dimers Linked at c-6 by the Shortest Bridge-a Sulfur Atom

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chinese chemical Letters 1997年第6期8卷 475-476页

作者： De Qi yuan kahee FUJITA Yuji OkABE (Faculty of Pharmaceutical Sciences, Nagasaki University, Bunkyo-machi 14-1, Nagasaki 852, Japan ) Faculty of Pharmaceutical Sciences Nagasaki University Bunkyo-machi 14-1 Nagasaki 852 Japan

Three cyclodextrin dimers bridged at c-6 by the shortest tether - a sulfur atom are synthesized by reacting cyclodextrin tosylates with Na2S and their binding properties are evaluated

关键词： ppm

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