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Human umbilical cord mesenchymal stem cells by hTERT gene transfection can differentiate into hepatocyte-like cells in vitro

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Human umbilical cord mesenchymal stem cells by hTERT gene tr...

Inflammasome links traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease

第二十次全国中西医结合肝病学术会议

作者： berthold Hocher 陈友鹏 Department of Infectious Diseasethe First Affiliated HospitalJinan University510630 GuangzhouGuangdongchina

The availability of a large amount of mesenchymal stem cells(MScs) would greatly advance liver-directed cell ***,MScs have a limited life span during culture in vitro. Therefore,we tested whether human umbilical cord mesenchymal stem cells(hUcMScs) could be immortalized by transduction with a lentiviral vectors carrying human telomerase catalytic subunit(hTERT) gene and investigated their differentiation *** results showed the transfected hUcMScs had over-expression of hTERT gene and up-regulated telomerase *** transfected hUcMScs maintained typical morphology and MSc-specific markers,and showed vigorous proliferation activity undergoing more than 100 population doublings(PDs) to ***, they differentiated into adipocytes and *** incubation with hepatogenic agents, the transfected hUcMScs differenciated into hepatocyte-like cells,and had expression of hepatic markers,such as albumin,alpha-fetoprotein,*** transfected hUcMScs also showed no tumor transformation in nude *** conclusion,telomerization of hUcMScs by hTERT overexpression extends their replicative life span without influencing differentiation potential and it offers opportunities for obtaining enough number of cells and for liver-directed therapies.

关键词： umbilical cord mesenchymal stem cells life span differentiation telomerase lentivirus

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Neural Regeneration Research 2025年第6期20卷 1644-1664页

作者： Gabriela Seplovich Yazan Bouchi Juan Pablo de Rivero Vaccari Jennifer c.Munoz Pareja Andrew Reisner Laura Blackwell Yehia Mechref Kevin K.Wang J.Adrian Tyndall Binu Tharakan Firas Kobeissy Department of Surgery Morehouse School of MedicineAtlantaGAUSA Department of Neurobiology Center for NeurotraumaMultiomics&Biomarkers(CNMB)Neuroscience InstituteMorehouse School of MedicineAtlantaGAUSA Department of Neurological Surgery and the Miami Project to cure Paralysis University of Miami Miller School of MedicineMiamiFLUSA Division of Pediatric critical care Department of PediatricsUniversity of Miami Miller School of MedicineMiamiFLUSA Department of Pediatrics Emory UniversityAtlantaGAUSA Department of Neurosurgery Children’s Healthcare of AtlantaAtlantaGAUSA Department of chemistry and Biochemistry Texas Tech UniversityLubbockTXUSA Morehouse School of Medicine AtlantaGAUSA

Traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease are three distinct neurological disorders that share common pathophysiological mechanisms involving neuroinflammation. One sequela of neuroinflammation includes the pathologic hyperphosphorylation of tau protein, an endogenous microtubule-associated protein that protects the integrity of neuronal cytoskeletons. Tau hyperphosphorylation results in protein misfolding and subsequent accumulation of tau tangles forming neurotoxic aggregates. These misfolded proteins are characteristic of traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease and can lead to downstream neuroinflammatory processes, including assembly and activation of the inflammasome complex. Inflammasomes refer to a family of multimeric protein units that, upon activation, release a cascade of signaling molecules resulting in caspase-induced cell death and inflammation mediated by the release of interleukin-1β cytokine. One specific inflammasome, the NOD-like receptor protein 3, has been proposed to be a key regulator of tau phosphorylation where it has been shown that prolonged NOD-like receptor protein 3 activation acts as a causal factor in pathological tau accumulation and spreading. This review begins by describing the epidemiology and pathophysiology of traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease. Next, we highlight neuroinflammation as an overriding theme and discuss the role of the NOD-like receptor protein 3 inflammasome in the formation of tau deposits and how such tauopathic entities spread throughout the brain. We then propose a novel framework linking traumatic brain injury, chronic traumatic encephalopathy, and Alzheimer's disease as inflammasomedependent pathologies that exist along a temporal continuum. Finally, we discuss potential therapeutic targets that may intercept this pathway and ultimately minimize long-term neurological decline.

关键词： Alzheimer's disease caspase-1 chronic traumatic encephalopathy inflammasomes neurodegeneration neuroinflammation NLRP1 NLRP3 pyroptosis tauopathy traumatic brain injury

Adipose-derived stem cells in cutaneous wound repair

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Plastic and Aesthetic Research 2018年第8期5卷 16-23页

作者： Bong-Sung Kim berthold Debye Justus P.Beier Department of Plastic Surgery Hand Surgery-Burn CenterUniversity Hospital RWTH AachenAachen 52074Germany

Growing interest in regenerative medicine and advances in adipose tissue research have led to the identification of mesenchymal stem cells in adipose tissue, so called adipose tissue-derived stem cells (AScs). Due to the simple and safe harvest technique as well as high regenerative capacity, AScs are regarded as a potential source for various indications including cutaneous wound repair. This review provides a short overview over mechanisms of ASc action in cutaneous wound repair and data regarding its clinical application. Mostly experimental data provide accruing evidence for the supportive effect of AScs in cutaneous wound healing by secretion of soluble factors, differentiation into keratinocyte and fibroblasts, neovascularization and interaction with myofibroblasts. A number of in vivo experiments also support a positive effect of AScs in different wound healing models. Furthermore, first clinical data evaluated the feasibility of AScs in the treatment of different wound healing pathologies, e.g., chronic ulcers and burn wounds. Although the majority of currently available data indicate a beneficial role of AScs in cutaneous wound repair, additional detailed experimental studies and larger, high-quality clinical trials are required to provide a reliable statement on the true value of AScs in this context.

关键词： cutaneous wound healing adipose-derived stem cells fat grafting regenerative medicine cell-based therapy

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超声解剖彩色图谱第2版

Impact of genetic variation of tumor necrosis factor-α on gestational hypertension

2014年

作者：（德）berthold Block 丁自海主审亓恒涛主译

《超声解剖彩色图谱（第2版）》的最大特点是以图像为主、文字为辅，且每个超声切面图像都配有与之匹配的简笔画示意图，标示清楚，一目了然。书中显示出了腹部“超声感兴趣器官”（及甲状腺）的所有标准切面，并分别通过横向扫描和纵... 详细信息

ISBN: (纸本)9787533172374

《超声解剖彩色图谱（第2版）》的最大特点是以图像为主、文字为辅，且每个超声切面图像都配有与之匹配的简笔画示意图，标示清楚，一目了然。书中显示出了腹部“超声感兴趣器官”（及甲状腺）的所有标准切面，并分别通过横向扫描和纵向扫描，标示了各个器官和结构在三维空间的解剖细节与超声的关系。另外，《超声解剖彩色图谱（第2版）》延续了欧美医学教材的编排特点，版面精美、简明扼要、细致人微。每幅超声图像都带有关于探头位置和移动方向的体标，每幅示意图都有该切面在三维器官模型上的位置。新版与前一版相比，整体内容（涉及器官）不变，但图片由原有的244对增加至278对，且原有图片大量更新。

关键词：超声应用人体解剖学图谱

来源：汇雅图书（南通市图书馆... 评论

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chinese Medical Journal 2006年第9期119卷 719-724页

作者： cHEN You-peng Thiemo Pfab Torsten Slowinski claus-Michael Richter Michael Godes berthold Hocher Division of Infectious Disease Department of Interal Medicine First Affiliated Hospital of Jinan University Jinan University Guangzhou 510632 China center for cardiovascular Research Medical School Charit6 Humboldt University of Berlin Berlin D-10115 Germany Institute of Pharmacology and Toxicology Medical School Charitd Humboldt University of Berlin Berlin D-10115 Germany

Background The mechanisms responsible for the pathogeneses of gestational hypertension and preeclampsia are unclear. Tumor necrosis factor-1α （TNF-1α） is a pro-inflammatory Th1-type cytokine. TNFA gene is located in the human leukocyte antigen （HLA） class Ⅲ region of the major histocompatibility complex （MHc） on chromosome 6. The high TNF-1α mRNA expression may be associated with the TNF2 （A） allele, which is the polymorphism of TNF-1α at position - 308 in promoter region. This study assessed whether the TNF2 （A） allele at position -308 plays a role in the alteration of blood pressure （BP） and urinary protein excretion during pregnancy. Methods The original prospective cohort study comprised 1623 pregnant women from January 2000 to October 2001. The G/A polymorphism was done by restriction fragment length polymorphism （RFLP） analysis with Nco I enzyme. Results The distributions of the G/A polymorphism of TNF-1α in the promoter region at position -308 were wild-type 72.4% and variant 27.6%, respectively. The frequency of TNF2 （A） allele was approximately 0.15 for caucasian pregnant women in the study. It was not significantly different in the distributions of genotypes and G/A allele frequencies among the three groups of pregnant women with gestational hypertension, preexisting hypertension and normal blood pressure （P〉0.05）. The maternal blood pressure in the third trimester was significantly higher in the group of women possessing the TNF2 （A） allele compared to homozygous for the TNF1 （G） allele （systolic BE P〈0.01 and diastolic BE P〈0.05）. The elevated blood pressure in the TNF2 （A） group was accompanied by higher urinary protein excretion in the third trimester （P〈0.05）. The blood pressure and urinary protein excretion did not change apparently between the two groups in the first and second trimesters （P〉0.05）. conclusions Maternal TNF2 （A） allele of TNF-1α promoter region at position -308 could play a role in the alteration of blood pressures and/or enhan

关键词： tumor necrosis factor-α polymorphism, restriction fragment length gestational hypertension

血管紧张素转化酶2-血管紧张素（1-7）-Mas受体轴在胚胎生长发育中的重要性

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中华围产医学杂志 2012年第7期15卷 445-447页

作者：陈友鹏李健 HOcHER berthold 暨南大学第一临床医学院感染科广州510630 暨南大学第一临床医学院妇产科广州510630 德国波茨坦大学营养学研究所

我国肥胖症、高血压和2型糖尿病等代谢性疾病的发病率正在迅速增加，而且发病年龄也趋向年轻化，但对于其病因还没有统一的认识。近年来提出的“健康与疾病的发育起源（developmental origins of health and disease，DOHaD）”学说，

关键词：胚胎生长发育血管紧张素转化酶受体代谢性疾病 2型糖尿病健康与疾病发病年龄肥胖症

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响应速率可调的新型比例溢流阀

液压与气动 1992年第3期16卷 29-30页

作者： berthold Klug 王捷毅郭维建

毫无疑问,我们现在有能力使液压技术更符合生态要求。液压系统工作时的冲击和随之而产生的噪声应象由压力峰值和振动而造成的外部泄漏一样尽可能少。遗憾的是后者还经常发生,这仍是液压系统中一个令人头痛的问题。液压设备下部安装的集... 详细信息

毫无疑问,我们现在有能力使液压技术更符合生态要求。液压系统工作时的冲击和随之而产生的噪声应象由压力峰值和振动而造成的外部泄漏一样尽可能少。遗憾的是后者还经常发生,这仍是液压系统中一个令人头痛的问题。液压设备下部安装的集油盘表明,在这方面还有许多工作要做。期望别人去解决这个问题是不现实的,我们必须自己来着手处理。

关键词：响应速率可调溢流阀比例溢流阀

Finite element analysis of the effect of interlayer on interfacial stress transfer in layered graphene nanocomposites

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Journal of Materials Science & Technology 2019年第6期35卷 1147-1152页

作者： c.c.Roach Y.c.Lu Department of Mechanical Engineering University of Kentucky

Understanding the roles of interlayers in reinforcement efficiencies by layered graphene is very important in order to produce strong and light graphene based nanocomposites. The present paper uses the finite element method to evaluate the interfacial strain transfers and reinforcement efficiencies in layered graphene-polymer composites. Results indicate that the presence of compliant interlayers in layered graphene plays significant roles in the transfers of strain/stress from matrix to graphene and subsequently the reinforcement effectiveness of layered graphene. In general, the magnitude of shear strain transferred onto the rigid graphene decreases as the thickness of the interlayer increases. This trend becomes insignificant as the graphene becomes sufficiently large(s>25,000). The shear strain at the interface of graphene-matrix is also greatly influenced by the interlayer modulus. A stiffer interlayer would result in a higher shear strain transferred on the graphene. The performance of the interlayers is further affected by the property of the composite and the architecture of the layered graphene stack. If a composite contains more graphene phase, the efficiency of reinforcement by a layered graphene becomes improved. If a graphene stack contains more interlayers, the effectiveness of reinforcement at the edges of the graphene becomes negatively affected.

关键词： Interlayer Layered graphene Nanocomposites Interfacial stress transfer Finite element method