Human umbilical cord mesenchymal stem cells by hTERT gene transfection can differentiate into hepatocyte-like cells in vitro
会议名称：《第二十次全国中西医结合肝病学术会议》
会议日期：2011年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学]
关 键 词：umbilical cord mesenchymal stem cells life span differentiation telomerase lentivirus
摘 要：The availability of a large amount of mesenchymal stem cells(MSCs) would greatly advance liver-directed cell ***,MSCs have a limited life span during culture in vitro. Therefore,we tested whether human umbilical cord mesenchymal stem cells(hUCMSCs) could be immortalized by transduction with a lentiviral vectors carrying human telomerase catalytic subunit(hTERT) gene and investigated their differentiation *** results showed the transfected hUCMSCs had over-expression of hTERT gene and up-regulated telomerase *** transfected hUCMSCs maintained typical morphology and MSC-specific markers,and showed vigorous proliferation activity undergoing more than 100 population doublings(PDs) to ***, they differentiated into adipocytes and *** incubation with hepatogenic agents, the transfected hUCMSCs differenciated into hepatocyte-like cells,and had expression of hepatic markers,such as albumin,alpha-fetoprotein,*** transfected hUCMSCs also showed no tumor transformation in nude *** conclusion,telomerization of hUCMSCs by hTERT overexpression extends their replicative life span without influencing differentiation potential and it offers opportunities for obtaining enough number of cells and for liver-directed therapies.