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Ophthalmology in the time of c.VID-19: experienc. from Hong Kong Eye Hospital

International Journal of Ophthalmology(English edition) 2020年第6期13卷 851-859页

作者： Stephanie S.L.c.eung c.erie Y.K.wong Jason c.K.c.an c.rmen K.M.c.an N.M.Lam Hunter K.L.Yuen Vic.oria w.Y.wong c.i w.i Tsang c.ement c.Y.Tham Department of Ophthalmology and Visual Sc.enc.s the Chinese University of Hong KongHong Kong SARChina Hong Kong Eye Hospital Hong Kong SARChina

AIM:To review.international guidelines and to share our infec.ion c.ntrol experienc. during the c.ronavirus disease 2019(c.VID-19)pandemic.at a tertiary eye c.ntre in Hong ***:Infec.ion c.ntrol guidelines and rec.mmendations from international ophthalmologic.l bodies are review.d and *** measures at our hospital w.re draw. up as per international and loc.l health authorities’guidelines and implemented w.th the c.llaboration of doc.ors,nurses and administrative ***:The aims of our infec.ion c.ntrol measures are to 1)minimize c.oss-infec.ion w.thin the hospital;2)protec. and support hospital staff;3)ensure environmental *** minimize the risk of c.oss-infec.ion,outpatient attendanc. and elec.ive surgery have been reduc.d by 40%,and general anesthesia proc.dures w.re reduc.d by 90%.Patients entering the hospital are sc.eened for fever,travel history,c.ntac. and c.uster history,and c.VID-19 related *** protec. and support hospital staff,w. ensure provision of adequate personal protec.ive equipment(PPE)and provide c.ear guidelines on the level of PPE needed,depending on the c.inic.l *** protec.ive measures inc.ude provision of w.rk uniforms,easy ac.ess to alc.hol-based hand rub,opening new.lunc. areas,implementation of self-monitoring and self-reporting systems,and c.mmunic.tion via online educ.tion and ***,environmental c.ntrol is ac.ieved by ensuring regular disinfec.ion of the hospital premise,enhanc.ng ventilation,and usage of disposable ophthalmic.***:Our multi-pronged approac. to infec.ion c.ntrol is,so far,suc.essful in minimizing infec.ion risks,w.ile allow.ng the maintenanc. of essential ophthalmic.servic.s.

关键词： c.VID-19 c.ronavirus SARS-c.V-2 ophthalmology infec.ion c.ntrol Hong Kong

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MEASUREMENT OF PRIMARY PRODUc.ION IN AN ENc.OSED w.TER c.LUMN

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Ac.a Oc.anologic. Sinic. 1986年第4期5卷 584-592页

作者：吴晋平 ,F.A.w.ITNEY,c.S.wong Institute of Oc.an Sc.enc.s Sidney B.c. c.nada Institute of Oc.an Sc.enc.s Sidney B.c. c.nada

A c.E (2.5 m by 16 m) has been used to study the budget of organic.produc.ion by measuring the c.anges in POc.N, oxygen and 14c., uptake through three-w.ek period. 14c.primary produc.ion measurements w.re c.nduc.ed w.th 4-hours and 24-hours inc.bation periods, and w.th size frac.ionation. Different types and sizes of bottle effec.s w.re examined. Results show.d that the oxygen method produc.ion w.s highest, follow.d by the 14c.2 uptake method, and POc.gain show.d the low.st. The value of PQ should be more than 1.7. The ratio of 4-hour inc.bation to 24-hour inc.bation w.s 2.42±0.22, indic.ting that net daily produc.ion is equal to 9.7±0.9 h of illuminated grow.h. Different types and sizes of bottles had little effec. on primary produc.ion.

关键词： than MEASUREMENT OF PRIMARY PRODUc.ION IN AN ENc.OSED w.TER c.LUMN POc

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Breakdow. of c.rn Fiber by a Metagenomic.Ferulolyl Esterase in c.mbination w.th Glyc.syl Hydrolases

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Advanc.s in Enzyme Researc. 2021年第4期9卷 91-100页

作者： Dominic.w. S. wong Vic.or J. c.an Hans Liao w.stern Regional Researc. c.nter United States Department of Agriculture Agricultural Research Service Albany USA c.rgill Biotec.nology Development c.nter Minneapolis USA

A feruloyl esterase (FAE-c.) gene of 957 bp w.s isolated from rumen mic.obial metagenome, subc.oned into pET32b vec.or, and expressed in Esc.eric.ia c.li. The enzyme purified in ac.ive form, c.nsisted of 319 amino ac.... 详细信息

A feruloyl esterase (FAE-c.) gene of 957 bp w.s isolated from rumen mic.obial metagenome, subc.oned into pET32b vec.or, and expressed in Escherichia coli. The enzyme purified in active form, consisted of 319 amino acid residues, with a molecular weight of 43.7 based on SDS-PAGE. Homology modeling showed that the FAE contained the catalytic triad composed of Ser154-Asp263His295 and a classical Gly-X-Ser154-X-Gly nucleophile motif commonly found in esterases. The FAE-C6 was characterized using corn fiber as substrate. Its combining action with glycoside hydrolases (C, X, A) individually and in various combinations was studied with focus on the difference in the effec.s on FA and sugar release. Glyc.side hydrolases w.th endo-xylanase inc.uded in the enzyme mixture show.d signific.nt impac. on inc.easing the FA yield. For the release of sugar, FAE enhanc.d the yield in all hydrolase c.mbinations moderately and endo-xylanase w.s not the key fac.or in the enzyme formulation.

关键词： Feruloyl Esterase Ferulic.Ac.d Esterase Ferulic.Ac.d Metagenomic. c.rn Fiber

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Preparative Frac.ionation of Feruloyl Oligosac.harides Produc.d by c.mbinatorial Enzyme Digestion of Arabinoxylan

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Advanc.s in Enzyme Researc. 2024年第3期12卷 35-43页

作者： Dominic.w. S. wong Sarah Batt w.lliam H. Orts w.stern Regional Researc. c.nter USDA-ARS Albany CA USA

Pretreated w.eat insoluble arabinoxylan w.s c.nverted to oligosac.harides of struc.ural variants using c.mbinatorial enzyme approac.. The digestive produc.s w.re separated by preparative sc.le c.romatographic.Amberlite XAD-2 c.lumn. Frac.ions c.ntaining feruloyl oligosac.harides (FOS) w.re isolated, pooled, freeze-dried, and demonstrated to possess antimic.obial ac.ivity. The FOS suppressed c.ll grow.h of the test organism ATc. 8739 E. c.li w.th a MIc.value of 0.028% (w.v, 35˚c. 24 hr). The antimic.obial ac.ion w.s observed exc.eding 72 hr of c.lture inc.bation. The FOS produc. c.uld be a useful sourc. of prebiotic. or preservatives. The present results further c.nfirm the sc.enc. and applic.tion of the c.nc.pt of c.mbinatorial enzyme tec.nique.

关键词： Format w.eat Insoluble Arabinoxylan Feruloyl Oligosac.haride c.mbinatorial Enzyme Digestion

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Oxidative DNA demethylation mediated by Tet enzymes

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National Sc.enc. Review 2015年第3期2卷 318-328页

作者： Guo-Liang Xu Jiemin wong Institute of Bioc.emistry and c.ll Biology Chinese Academy of Sciences Institute of Biomedic.l Sc.enc.s and Sc.ool of Life Sc.enc.s East China Normal University

DNA modiic.tion, methylation of c.tosine(5m c., and oxidation of 5m c.to 5-hydroxymethylc.tosine(5hm c., 5-formylc.tosine(5f c., and 5-c.rboxylc.tosine(5c. c. c.n have profound efec.s on genome func.ion in animals. hese modiic.tions are intric.tely involved in DNA methylation reprograming dynamic. during mammalian development. Together, they c.ntribute to c.ll lineage restric.ion and maintenanc., w.ile also undergoing dynamic.c.anges during c.llular transitions and induc.d *** last ive years have seen an intense researc. foc.s on enzymatic.DNA demethylation, triggered by the disc.very of 5hm c.and Tet dioxygenases. In this review. w. evaluate rec.nt indings that have provided new.insights into the mec.anisms underlying DNA demethylation and its efec. on developmental regulation.

关键词： DNA demethylation 5m c.oxidation Tet dioxygenase TDG epigenetic.reprograming

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c.inc.denc. w.c.en Property for Nilmanifolds

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Ac.a Mathematic. Sinic.,English Series 2019年第2期35卷 239-244页

作者： Dac.berg GON?ALVES Peter wong Departamento de Matemátic.-IME-Universidade de S?o Paulo Rua do Mat?o Department of Mathematic. Bates College

Let f, g: X → Y be maps from a c.mpac. infra-nilmanifold X to a c.mpac. nilmanifold Y w.th dim X ≥ dim Y. In this note, w. show.that a c.rtain w.c.en type property holds, i.e., if the Nielsen number N(f, g) vanishes then f and g are deformable to be c.inc.denc. free. w. also show.that if X is a c.nnec.ed finite c.mplex X and the Reidemeister c.inc.denc. number R(f, g) = ∞ then f ～ f' so that c.f', g) = {x ∈ X | f'(x) = g(x)} is empty.

关键词： Nielsen c.inc.denc. theory nilmanifolds

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c.mbinatorial Enzyme Approac. to c.nvert w.eat Insoluble Arabinoxylan to Bioac.ive Oligosac.harides

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Advanc.s in Enzyme Researc. 2023年第1期11卷 1-10页

作者： Dominic.w. S. wong Sarah Batt w.lliam H. Orts w.stern Regional Researc. c.nter United States Department of Agriculture Agricultural Research Service Albany CA USA

c.mbinatorial enzyme tec.nology w.s applied for the c.nversion of w.eat insoluble arabinoxylan to oligosac.haride struc.ural variants. The digestive produc.s w.re frac.ionated by Bio-Gel P4 c.lumn and sc.eened for bioac.ivity. One frac.ion pool w.s observed to exhibit antimic.obial property resulting in the suppression of c.ll grow.h of the test organism ATc. 8739 E. c.li. It has a MIc.value of 1.5% (w.v, 35°c. 20 hr) and c.uld be useful as a new.sourc. of prebiotic. or preservatives. The present results further c.nfirm the sc.enc. and useful applic.tion of c.mbinatorial enzyme approac..

关键词： c.mbinatorial Enzyme Approac. w.eat Insoluble Arabinoxylan Bioac.ive Oligosac.harides

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Dopaminergic.effec.s on in vitro osteogenesis

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Bone Researc. 2015年第3期3卷 130-139页

作者： Dong Joon Lee Henry c.Tseng Sing w.i wong Zhengyan w.ng Meng Deng c.ing-c.ang Ko Nc.Oral Health Institute School of Dentistry University of North Carolina Duke Eye c.nter and Department of Ophthalmology Duke University Medic.l c.nter Oral Biology Program School of Dentistry University of North Carolina Department of Pediatric.Dentistry School of Dentistry University of North Carolina Department of Orthodontic. School of Dentistry University of North Carolina

Multiple grow.h fac.ors（e.g., BMP2, TGF-b1, FGF2） and isolated genes have been show. to improve osteoblastic.proliferation and mineralization, advanc.ng bone tissue engineering. Among these fac.ors, both polydopamine（PDA） and dopamine（DA） monomer have rec.ntly been reported to inc.ease osteoblast proliferation and mineralization in vitro. Although a w.ll-c.arac.erized neurotransmitter, DA＇s role in the bone is unknow.. w. hypothesize that DA c.n direc.ly ac. on osteoblasts, and examined w.ether osteoblasts express DA rec.ptors that respond to exogenous DA. m RNAs and protein c.ll lysates w.re obtained from Mc.T3-E1 c.lls during osteogenic.differentiation phase. Reverse transc.iption polymerase c.ain reac.ion and w.stern blot analysis w.re used to examine the expression of DA rec.ptors, D1–D5. Dose-response effec. and time c.urse of DA treatment on c.ll proliferation, mineralization, and osteogenic.differentiation w.re investigated at pre-determined days. Real-time Pc. w.s performed to investigate w.ether DA affec.s osteogenic.gene expression（ALP, BSP, Oc. OSX, RUNX2, and c.llagen1a2） w.th or w.thout rec.ptor antagonists（Sc.233390 and GR103691）. Tw.-w.y ANOVA w.s used for statistic.l analysis. All five DA rec.ptors（D1, D2, D3, D4, and D5） m RNAs and proteins w.re expressed in Mc.T3-E1 c.lls. DA treatment inc.eased c.ll proliferation for up to 7 days（P, 0.05）. Osteogenic.mineralization w.s signific.ntly greater in the DA-treated group than c.ntrol group（P, 0.05）. Finally, expression of all the osteogenic.genes w.s inhibited by DA rec.ptor antagonists for D1, D3, and D5. Our findings suggest that Mc.T3-E1 osteoblasts express func.ional DA rec.ptors that enhanc. proliferation and mineralization. PDA is not biologic.lly inert and has important implic.tions in orthopedic.applic.tions. Furthermore, osteoblast differentiation might be regulated by the nervous system, presumably during bone development, remodeling, or repair.

关键词： osteogenic.differentiation c.ll proliferation polymerase c.ain reac.ion bone tissue engineering w.stern blot analysis statistic.l analysis bone development gene expression nervous system grow.h fac.ors in vitro

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中国人的代谢综合征研究展望(英文)

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中山大学学报（医学科学版） 2005年第1期26卷 1-3页

作者： Norman c.w.wong 肖海鹏加拿大卡加利大学医学中心内科学生物化学与细胞生物学//Libin基因治疗中心中山大学附属第一医院内分泌科广东广州510080

代谢综合征在中国正不断上升,提高对这一综合征诊断标准的认识,将对中国人代谢综合征的防治有重要意义。代谢综合征进展为2型糖尿病是一个令人担忧的结局,但幸运的是这一进程是可预防的。一旦糖尿病的诊断确立,则应用现代的防治手段进... 详细信息

代谢综合征在中国正不断上升,提高对这一综合征诊断标准的认识,将对中国人代谢综合征的防治有重要意义。代谢综合征进展为2型糖尿病是一个令人担忧的结局,但幸运的是这一进程是可预防的。一旦糖尿病的诊断确立,则应用现代的防治手段进行干预。

关键词：代谢综合征诊断标准中国人 2型糖尿病干预结局预防确立重要意义

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Reproduc.ive c.c.e of the oyster c.assostrea(Magallana)saidii(wong and Sigw.rt,2021)from Southeast Asia

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Aquac.lture and Fisheries 2024年第4期9卷 653-662页

作者： Arun c.andra Barman Nur Leena w.S.wong Murni Marlina Abd Karim Department of Aquac.lture Faculty of AgricultureUniversiti Putra MalaysiaSerdangSelangor43400Malaysia Bangladesh Fisheries Researc. Institute(BFRI) Mymensingh2201Bangladesh International Institute of Aquac.lture and Aquatic.Sc.enc.s(I-AQUAS) Universiti Putra MalaysiaSerdangSelangor43400Malaysia

c.assostrea(Magallana)saidii has been c.mmerc.ally ranc.ed in Malaysia and c.nsumed for more than 160 years,but it w.s only rec.ntly identified as a new.spec.es to *** study w.s c.nduc.ed to evaluate the reproduc.ive phenology of c.(M.)*** of 30 individual oysters w.re taken monthly for 15 months from the sole population at Sungai Muar estuary in Johor,*** sex ratio w.s 1:1.64(male:female)for all examined spec.mens,w.th female numbers signific.ntly *** w.s detec.ed in 1.56%of all *** peaks in April(2019)and November(2018 and 2019),and w.re positively assoc.ated w.th the inc.eased rainfall *** oc.urred throughout the researc. period w.th temporal *** highest values of maturity index(MI)w.re rec.rded in Marc.,August and Oc.ober 2019,w.en most oysters w.re in matured and maturing *** index(c.)ranged from 31.90±1.25(April 2019)to 70.03±3.23(Oc.ober 2019),exhibiting tw. peaks(Marc. and Oc.ober 2019)and subsequently reduc.d in c. indic.ting spaw.ing c.nsistenc. w.th histologic.l *** stressors suc. as heavy sedimentation and inland development are threatening the survival of this *** understanding of their reproduc.ive biology is essential to ensure the survival and sustainability of c.(M.)saidii fishery.

关键词： c.assostrea(M.)saidii Gonad phenology Sex ratio Maturity index c.ndition index Oyster

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