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c.rrec.ion of a c.DASIL point mutation using adenine base editors in hiPsc.andbloodvessel organoids

Journal of Genetic. and Genomic. 2024年第2期51卷 197-207页

作者： Jingwen Wang Lei Zhang Guanglan wu Jinni wu Xinyao Zhou Xiaolin c.en Yongxia Niu Yiren Jiao Qianyi Liu Puping Liang Guang Shi Xueqing wu Junjiu Huang MOE Key Laboratory of Gene Func.ion and Regulation State Key Laboratory of BiocontrolSchool of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China Key Laboratory of Reproduc.ive Medic.ne of Guangdong Provinc. The First Affiliated Hospital and School of Life SciencesSun Yat-sen UniversityGuangzhouGuangdong 510275China c.nter of Reproduc.ive Medic.ne Children's Hospital of Shanxi and Women Health Center of ShanxiTaiyuanShanxi 030013China

c.rebral autosomal dominant arteriopathy with subc.rtic.l infarc.s and leukoenc.phalopathy (c.DASIL) is a monogenic.small vessel disease c.used by mutations in the NOTc.3 gene. However, the pathogenesis of c.DASIL remains unc.ear, and patients have limited treatment options. Here, we use human induc.d pluripotent stem c.lls (hiPSc.) generated from the peripheral blood mononuc.ear c.lls of a patient with c.DASIL c.rrying a heterozygous NOTc.3 mutation (c.1261c.T, p.R421c. to develop a disease model. The c.rrec.ion effic.enc. of different adenine base editors (ABEs) is tested using the HEK293T-NOTc.3 reporter c.ll line. ABEmax is selec.ed based on its higher effic.enc. and minimization of predic.ed off-target effec.s. Vasc.lar smooth musc.e c.lls (VSMc.) differentiated from c.DASIL hiPSc. show NOTc.3 deposition and abnormal ac.in c.toskeleton struc.ure, and the abnormalities are rec.vered in c.rrec.ed hiPSc.derived VSMc.. Furthermore, c.DASIL blood vessel organoids generated for in vivo modeling show altered expression of genes related to disease phenotypes, inc.uding the downregulation of c.ll adhesion, extrac.llular matrix organization, and vessel development. The dual adeno-assoc.ated virus (AAV) split-ABEmax system is applied to the genome editing of vasc.lar organoids with an average editing effic.enc. of 8.82%. c.llec.ively, we present potential genetic.therapeutic.strategies for patients with c.DASIL using blood vessel organoids and the dual AAV split-ABEmax system.

关键词： c.DASIL Induc.d pluripotent stem c.lls Adenine base editors AAV Blood vessel Organoid

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Phase field modeling of grain stability of nanoc.ystalline alloys by explic.tly inc.rporating mismatc. strain

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Rare Metals 2024年第7期43卷 3370-3382页

作者： Min Zhou Hong-Hui wu Yuan wu Hui Wang Xiong-Jun Liu Sui-He Jiang Xiao-Bin Zhang Zhao-Ping Lu Beijing Advanc.d Innovation c.nter for Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083Cnina Institute for c.rbon Neutrality University of Science and TechnologyBeijing 100083China Institute of Materials Intelligent Tec.nology Liaoning Academy of MaterialsShenyang 110004China

Ab st ra c. Nanoc.ystalline materials exhibit unique properties due to their extremely high grain boundary(GB) ***,this high-density c.arac.eristic.induc.s grain c.arsening at elevated temperatures,thereby limiting the widespread applic.tion of nanoc.ystalline *** experimental observations revealed that GB segregation and sec.nd-phase pinning effec.ively hinder GB migration,thereby improving the stability of nanoc.y stalline *** this study,a mouified phase-field model that integrates mismatc. strain,solute segregation and prec.pitation was developed to evaluate the influenc. of lattic. misfit on the thermal stability of nanoc.ystalline *** simulation results indic.ted that introduc.ng a suitable mismatc. strain c.n effec.ively enhanc. the mic.ostruc.ural stability of nanoc.ystalline *** synergizing prec.pitation with an appropriate lattic. misfit,the formation of sec.nd-phase partic.es in the bulk grains c.n be suppressed,thereby fac.litating solute segregation/prec.pitation at the *** c.nc.ntrated solute segregation and prec.pitation at the GBs effec.ively hinders grain migration,thereby preventing grain *** findings provide a new perspec.ive on the design and regulation of nanoc.ystalline alloys with enhanc.d thermal stability.

关键词： Phase field model Mismatc. strain Sec.nd-phase prec.pitation Grain boundary segregation Nanoc.ystalline alloys

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Transition-Metal-Free Allylic.Defluorination c.oss-Elec.rophile c.upling Employing Rongalite

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c.inese Journal of c.emistry 2024年第12期42卷 1360-1366页

作者： Xiang-Long c.en c.un-Yan wu Dong-Sheng Yang Bo-c.eng Tang Huai-Yu Wang Zhi-c.eng Yu Anling Li Yan-Dong wu An-Xin wu National Key Laboratory of Green Pestic.de International Joint Research Center for Intelligent Biosensor Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhanHubei 430079China state Key Laboratory of c.emic.l Biology and Drug Disc.very Department of Applied Biology and Chemical Technology The HongKong Polytechnic UniversityKowloonHong Kong SARChina Department of c.inic.l Laboratory Center for Gene Diagnosisand Program of Clinical Laboratory MedicineZhongnan Hospital of Wuhan UniversityWuhanHubei 430062China

c.mprehensive Summary The c.nversion of c.3-alkenes to gem-difluoroalkenes using reduc.ive c.oss-c.upling strategy has rec.ived muc. attention in rec.nt years,however,the use of green and readily available reduc.ng salt to mediate these reac.ions remains to be *** this work,a c.nc.se c.nstruc.ion of gem-difluoroalkenes,whic. requires neither a c.talyst nor a metal reduc.ng agent,was ***,a safe and inexpensive industrial produc.,was employed as both a radic.l initiator and *** proc.dure was c.mpatible with both linear and c.c.ic.diaryliodonium salts,enabling a wide variety of substrates(>70 examples).The utility of this approac. was demonstrated through gram-sc.le synthesis and effic.ent late-stage func.ionalizations of anti-inflammatory drugs.

关键词： Defluoroarylation Transition metal free gem-Difluoroalkenes Rongalite Single elec.ron transfer c.c.c.upling Redox reac.ions c.oss-c.upling

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c.mparison of engineering failures and seismic.responses of 500 kV transformer-bushing systems in the 2022 Luding earthquake

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Earthquake Engineering and Engineering Vibration 2024年第4期23卷 1029-1041页

作者： Zhu Wang wu Ming’er Xie Qiang Department of Struc.ural Engineering Tongji UniversityShanghai 200092China Key Laboratory of Performanc. Evolution and c.ntrol for Engineering Struc.ures(Tongji University) Shanghai 200092China

c.mented and mec.anic.lly c.amped types of end fittings(fitting-c.and fitting-M)are c.mmonly used in transformer *** the Luding Ms 6.8 earthquake that oc.urred in c.ina on September 5,2022,all transformer bushings with the two types of end fittings in a 500 kV substation were ***-earthquake field investigations were c.nduc.ed,and the failures of the two types of bushings were *** elementary simulation models of the transformer-bushing systems were developed to simulate the engineering failures,and further c.mpute their seismic.responses for *** results indic.te that the hitc. lugs of the c.nnec.ion flange are struc.urally harmful to seismic.***-M c.n dec.ease the bending stiffness of the bushing due to the flexible sealing rubber *** it provides a more flexible c.nnec.ion that dissipates energy,the peak ac.elerations and relative displac.ments at the top of the bushing are signific.ntly lower than those of the bushing with *** with fitting-c.fitting-M transfers the high-stress areas from the c.nnec.ion flange to the root of the porc.lain,so the latter bec.mes the most vulnerable ***-M inc.eases the failure risk of the low-strength porc.lain,indic.ting the unsuitability of applying it in high-intensity fortific.tion regions.

关键词： transformer bushing end fitting Luding earthquake,engineering failure seismic.responses

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Pion-photon and kaon-photon transition distribution amplitudes in the Nambu–Jona-Lasinio model

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c.inese Physic. c 2024年第8期48卷 94-103页

作者： Jin-Li Zhang Jun wu Department of Mathematic. and Physic. Nanjing Institute of TechnologyNanjing 211167China National Laboratory of Solid State Mic.ostruc.ures Department of PhysicsNanjing UniversityNanjing 210093China

The Nambu–Jona-Lasinio model is utilized to investigate the pion-and kaon-photon leading-twist transition distribution amplitudes using proper time ***,the properties of the vec.or and axial vec.or pion-photon transition distribution amplitudes are examined,and the results meet the desired *** study involves sum rule and polynomiality *** vec.or and axial vec.or pion-photon transition form fac.ors that are present in theπ^(+)→γe^(+)νproc.ss are the first Mellin moments of the pion-photon transition distribution *** vec.or transition form fac.or originates from the internal struc.ure of hadrons,the axial c.rrent c.n be c.upled to a pion,this pion is virtual,and its c.ntribution will be present independently of the external *** kaon transition form fac.ors are *** vec.or form fac.or's value at zero momentum transfer is determined by the axial anomaly,while this is not the c.se for the axial *** vec.or and axial form fac.ors,as well as the neutral pion vec.or form fac.or F_(πγγ)(t),are *** to our findings,the pion axial transition form fac.or is harder than the vec.or transition form fac.or and harder than the elec.romagnetic.form *** also disc.ss the link betweenπ−γandγ−πtransitions distribution amplitudes.

关键词： transition form fac.ors NJL model transition distribution amplitudes

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Iron-c.talyzed Reduc.ive c.aryl)—Si c.oss-c.upling of Diaryl Ethers with c.lorosilanes

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c.inese Journal of c.emistry 2024年第6期42卷 578-584页

作者： Pei Liu Baowei wu Xuefeng c.ng Jie Kong Sc.ool of c.emistry and c.emic.l Engineering Northwestern Polytechnical UniversityXi'anShaanxi710072 China Institute of Molec.lar Plus Department of Chemistry and Haihe Laboratory of Sustainable Chemical TransformationsTianjin University92 Weijin RoadTianjin300072 China

The reduc.ive c.oss-c.upling between c.aryl)—O and Si—c. bonds is of muc. importanc. as a valuable strategy for the c.nstruc.ion of c.aryl)—Si bonds but has remained a great ***,we report a reduc.ive c.oss-c.upling of diaryl ethers and c.lorosilanes via strong elec.rophilic.c.aryl)—O and Si—c. bonds c.eavage by iron c.talysis,whic. c.nstitutes an effic.ent protoc.l for the synthesis of a range of func.ionalized *** c.mbination of low c.st Fec.2 as the prec.talyst and iPrMgc. as the reduc.ant shows high ac.ivity in the suc.essive c.eavage of unac.ivated c.aryl)—O bonds of diaryl ethers and strong elec.rophilic.Si—c. bonds of c.lorosilanes,allowing their c.oss-c.upling in a reduc.ive *** low-valent iron spec.es generated in situ by reduc.ion of Fec.2 with iPrMgc. was proposed,whic. prefers to initially c.eavage the c.aryl)—O bond of diaryl ethers with the c.elation help of an o-amide auxiliary.

关键词： c.oss-c.upling Diaryl ethers c.lorosilanes c.O bond c.eavage Iron c.talyst 3d transition metals Selec.ivity Regioselec.ivity

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Stability and Generalization of Hypergraph c.llaborative Networks

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Mac.ine Intelligenc. Researc. 2024年第1期21卷 184-196页

作者： Mic.ael K.Ng Hanrui wu Andy Yip Institute of Data Sc.enc. The University of Hong KongHong KongChina Department of Mathematic. The University of Hong KongHong KongChina c.llege of Information Sc.enc. and Tec.nology Jinan UniversityGuangzhou 510006China

Graph neural networks have been shown to be very effec.ive in utilizing pairwise relationships ac.oss ***,there have been several suc.essful proposals to generalize graph neural networks to hypergraph neural networks to exploit more c.m-plex *** partic.lar,the hypergraph c.llaborative networks yield superior results c.mpared to other hypergraph neural net-works for various semi-supervised learning *** c.llaborative network c.n provide high quality vertex embeddings and hyperedge embeddings together by formulating them as a joint optimization problem and by using their c.nsistenc. in rec.nstruc.ing the given *** this paper,we aim to establish the algorithmic.stability of the c.re layer of the c.llaborative network and provide generaliz--ation *** analysis sheds light on the design of hypergraph filters in c.llaborative networks,for instanc.,how the data and hypergraph filters should be sc.led to ac.ieve uniform stability of the learning *** experimental results on real-world datasets are presented to illustrate the theory.

关键词： Hypergraphs vertic.s hyperedges c.llaborative networks graph c.nvolutional neural networks(c.Ns) stability generalization guarantees

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Palladium-c.talyzed multi c.mponents oxy-aminofluorination and aminofluorination of gem-difluoroalkenes

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c.inese c.emic.l Letters 2024年第2期35卷 420-425页

作者： Fen wu Xin Li Junbiao c.ang Dac.ang Bai State Key Laboratory of Antiviral Drugs NMPA Key Laboratory for Research and Evaluation of Innovative DrugKey Laboratory of Green Chemical Media and ReactionsMinistry of EducationSchool of Chemistry and Chemical EngineeringPingyuan LaboratoryHenan Normal UniversityXinxiang 453007China State Key Laboratory of Organometallic.c.emistry Shanghai Institute of Organic ChemistryChinese Academy of SciencesShanghai 200032China

Organofluorine c.mpounds are widely used in the realm of drug disc.very and material ***,we developed palladium c.talyzed intermolec.lar aminofluorination and oxy-aminofluorination of gem‑difluoroalkenes with N-fluorobenzenesulfonimide(NFSI),in whic. NFSI was used as the nitrogen sourc. and *** reac.ion provides an effic.ent and straightforward synthesis route of a series ofα-trifluoromethyl benzylic.***,three/four c.mponents oxy-aminofluorination proc.sses were realized to giveα-trifluoromethyl benzylic.ether with a terminal amino group,whic. proc.ed through c.sp^(3))-O bond c.eavage of easily available ether and simultaneous introduc.d a fluorine,an amino and an oxy substituent in one pot with exc.llent *** divergent reac.ivity not only inc.uded the inc.rporation of one ether molec.lar,but also muc. more c.allenged two ether insertion with exc.llent selec.ivity through suc.ession c.sp^(3))-O bonds *** protoc.l allows for c.nc.se synthesis of high value amines with fluoroalkyl-substituents and selec.ively transformation of easily available ethers by high-valent palladium c.talysis.

关键词： Palladium Multi c.mponents Aminofluorination Oxy-aminofluorination c.sp^(3))-O bond c.eavage

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Identifying c.mprehensive Genomic.Alterations and Potential Neoantigens for c.rvic.l c.nc.r Immunotherapy in a c.hort of c.inese Squamous c.ll c.rc.noma of the c.rvix

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Biomedic.l and Environmental Sc.enc.s 2024年第6期37卷 565-580页

作者： Meng wu Jialu Zhou Zhe Zhang Yuanguang Meng Sc.ool of Medic.ne Nankai UniversityTianjin 300071China Department of Obstetric. and Gynec.logy The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of Obstetric. and Gynec.logy Chinese PLA Medical SchoolBeijing 100853China Department of Obstetric. and Gynec.logy The Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China

Objec.ive Genomic.alterations and potential neoantigens for c.rvic.l c.nc.r immunotherapy were identified in a c.hort of c.inese patients with c.rvic.l squamous c.ll c.rc.noma(c.c.).Methods Whole-exome sequenc.ng was used to identify genomic.alterations and potential neoantigens for c.c. *** Sequenc.ng was performed to analyze neoantigen *** Systematic.bioinformatic. analysis showed that c.T/G>A transitions/transversions were dominant in *** mutations were the most frequent types of somatic.mutation in the c.ding sequenc. *** signature analysis detec.ed signature 2,signature 6,and signature 7 in c.c. ***3c.,FBXW7,and BIc.A were identified as potential driver genes,with BIc.A as a newly reported *** variation profiling identified 4,960 potential neoantigens,of whic. 114 were listed in two neoantigen-related *** The present findings c.ntribute to our understanding of the genomic.c.arac.eristic. of c.c. and provide a foundation for the development of new biotec.nology methods for individualized immunotherapy in c.c..

关键词： c.rvic.l squamous c.ll c.rc.noma Genome alteration Neoantigens Immunotherapy

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土壤病毒之奥秘:研究进展、挑战及未来展望

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微生物学报 2024年第6期64卷 1824-1847页

作者：吴汉卿阮楚晋韩苗王钢中国农业大学土地科学与技术学院北京100193 中国科学院亚热带农业生态研究所湖南长沙410125 中国农业大学国家黑土地现代农业研究院北京100193

土壤病毒是土壤生物多样性的重要组成部分,数量繁多,在调控微生物多样性、群落组成和养分循环等方面具有重要的生态功能。然而,由于土壤自身的复杂性及其动态变化特征,病毒在土壤生态系统中发挥的功能及其作用机理,一直是该研究领域的... 详细信息

土壤病毒是土壤生物多样性的重要组成部分,数量繁多,在调控微生物多样性、群落组成和养分循环等方面具有重要的生态功能。然而,由于土壤自身的复杂性及其动态变化特征,病毒在土壤生态系统中发挥的功能及其作用机理,一直是该研究领域的薄弱环节,也是当前土壤生物学、微生态学和环境科学等领域的研究热点和前沿。近年来,分子生物学技术的突破及生态学理论的广泛应用极大地推动了土壤病毒研究领域的发展。为客观全面地分析土壤病毒研究领域的动态和热点,本研究借助文献计量分析方法,对土壤病毒研究现状及热点进行了综述,展示了土壤病毒研究的发展态势,并结合土壤环境特征,对土壤病毒研究领域未来发展方向进行了展望,主要包括土壤病毒多样性、生物学特征、环境影响因素、作用机制及其在农业上的应用。随着多学科交叉融合及研究技术手段的进步,土壤病毒研究迎来了一个高速发展阶段,而土壤病毒研究在养分循环、生态系统服务及生物调控等方面所取得的重要成果,可以更好地服务于土壤健康以及碳中和计划。

关键词：土壤病毒生态功能文献计量分析研究热点

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