检索结果-南通市图书馆

Exploiting biased reptation for continuous flow preparative DNA fractionation in a versatile microfluidic platform

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2017年第1期3卷 294-301页

作者： Burcu Gumuscu Johan G.Bomer Hans L.de Boer Albert van den Berg Jan c.T.Eijkel BIOS Lab-on-a-chip Group MESA+Institute for NanotechnologyMIRA Institute for Biomedical Technology and Technical MedicineUniversity of Twente7500 AE EnschedeThe Netherlands

A new approach is presented for preparative,continuous flow fractionation of sub-10-kbp DNA fragments,which exploits the variation in the field-dependent mobility of the DNA molecules based on their *** pulsed electric fields of significantly different magnitudes are applied to a microchip filled with a sieving matrix of 1.2% agarose *** this method,we demonstrate a high-resolution separation of 0.5,1,2,5,and 10 kbp DNA fragments within 2 *** the separation,DNA fragments are also purified from other ionic *** fractionation of sub-10-kbp DNA molecules plays an important role in second-generation *** presented device performs rapid high-resolution fractionation and it can be reliably manufactured with simple microfabrication procedures.

关键词： agarose gel biased reptation continuous flow DNA separation DNA purification preparative fractionation

A collagen plug with shape memory to seal iatrogenic fetal membrane defects after fetoscopic surgery

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第2期20卷 463-471页

作者： Rob T.c.Meuwese Elly M.M.Versteeg Joris van Drongelen Daniëlle de Hoog Debora Bouwhuis Frank P.H.A.vandenbussche Toin H.van Kuppevelt Willeke F.Daamen Department of Biochemistry Radboud Institute for Molecular Life SciencesRadboud University Medical CenterPO Box 91016500 HBNijmegenthe Netherlands Department of Obstetrics&Gynecology Radboud University Medical CenterPO Box 91016500 HBNijmegenthe Netherlands Department of Obstetrics&Gynecology Helios Klinikum DuisburgAn der Abtei 747166DuisburgGermany

Iatrogenic preterm premature rupture of fetal membranes(iPPROM)after fetal surgery remains a strong trigger for premature *** fetal membrane defects do not heal spontaneously and amniotic fluid leakage and chorioamniotic membrane separation may occur,we developed a biocompatible,fetoscopically-applicable collagen plug with shape memory to prevent *** plug expands directly upon employment and seals fetal membranes,hence preventing amniotic fluid leakage and potentially *** typeⅠcollagen plugs were given shape memory and crimped to fit through a fetoscopic cannula(Ø3 mm).Expansion of the plug was examined in phosphate buffered saline(PBS).Its sealing capacity was studied ex vivo using human fetal membranes,and in situ in a porcine bladder *** crimped plug with shape memory expanded and tripled in diameter within 1 min when placed into PBS,whereas a crimped plug without shape memory did *** both human fetal membranes and porcine bladder,the plug expanded in the defect,secured itself and sealed the defect without membrane *** conclusion,collagen plugs with shape memory are promising as medical device for rapid sealing of fetoscopic defects in fetal membranes at the endoscopic entry point.

关键词： Biomaterial crosslinking Fetoscopy Fetal therapy Medical device Regenerative medicine

Sustainable Application of a Novel Water cycle Using Seawater for Toilet Flushing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2016年第4期2卷 460-469页

作者： Xiaoming Liu Ji Dai Di Wu Feng Jiang Guanghao chen Ho-Kwong chui Mark c. M. van Loosdrecht Department of civil and Environmental Engineering The Hong Kong University of Science and Technology Hong Kong China Water Technology center The Hong Kong University of Science and Technology Hong Kong China chinese National Engineering Research center for control and Treatment of Heavy Metal Pollution (Hong Kong Branch) The Hong Kong University of Science and Technology l-long Kong China School of chemistry and Environment South China Normal University Guangzhou 510006 China Department of Biotechnology Delft University of Technology Delft2629 HZ the Netherlands

Global water security is a severe issue that threatens human health and well-being. Finding sustainable alternative water resources has become a matter of great urgency. For coastal urban areas, desalinated seawater could serve as a freshwater supply. However, since 20%-30% of the water supply is used for flushing waste from the city, seawater with simple treatment could also partly replace the use of freshwater. In this work, the freshwater saving potential and environmental impacts of the urban water system （water-wastewater closed loop） adopting seawater desalination, seawater for toilet flushing （SWTF）, or reclaimed water for toilet flushing （RWTF） are compared with those of a conventional freshwater system, through a life-cycle assessment and sensitivity analysis. The potential applications of these processes are also assessed. The results support the environmental sustainability of the SWTF approach, but its potential application depends on the coastal distance and effective population density of a city. Developed coastal cities with an effective population density exceeding 3000 ***-2 and located less than 30 km from the seashore （for the main pipe supplying seawater to the city） would benefit from applying SWTF, regardless of other impact parameters. By further applying the sulfate reduction, autotrophic denitrification, and nitrification integrated （SANI） process for wastewater treatment, the maximum distance from the seashore can be extended to 60 km. considering that most modern urbanized cities fulfill these criteria, the next generation of water supply systems could consist of a freshwater supply coupled with a seawater supply for sustainable urban development.

关键词： Alternative water resources Seawater toilet flushing SANIUrban water system Life-cycle assessment

Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate in Escherichia coli

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2023年第4期8卷 629-639页

作者： Karel Olavarria Marco V.Becker Diana Z.Sousa Mark c.M.van Loosdrecht S.Aljoscha Wahl Laboratory of Microbiology Wageningen University and ResearchStippenenweg 46708 WEWageningenThe Netherlands centre for Living Technologies Eindhoven-Wageningen-Utrecht AlliancePrincetonlaan 63584 CBUtrechtThe Netherlands Department of Biotechnology Applied Sciences FacultyDelft University of Technologyvan der Maasweg 92629 HZDelftThe Netherlands Lehrstuhl für Bioverfahrenstechnik Friedrich-Alexander-Universit¨atPaul-Gordan-Strasse 391052ErlangenGermany

Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages,such as increased yields and reduced energy ***,lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic *** stoichiometric analyses and genetic modifications are frequently employed in metabolic engineering,the use of thermodynamic tools to evaluate the feasibility of planned interventions is less *** this study,we propose a novel metabolic engineering strategy to achieve an efficient anaerobic production of poly-(R)-3-hydroxybutyrate(PHB)in the model organism Escherichia *** approach involves re-routing of two-thirds of the glycolytic flux through non-oxidative glycolysis and coupling PHB synthesis with NADH *** complemented our stoichiometric analysis with various thermodynamic approaches to assess the feasibility and the bottlenecks in the proposed engineered *** to our calculations,the main thermodynamic bottleneck are the reactions catalyzed by the acetoacetyl-coAβ-ketothiolase(Ec 2.***.1.9)and the acetoacetyl-coA reductase(Ec 1.***.1.36).Furthermore,we calculated thermodynamically consistent sets of kinetic parameters to determine the enzyme amounts required for sustaining the conversion *** the case of the engineered conversion route,the protein pool necessary to sustain the desired fluxes could account for 20%of the whole cell dry weight.

关键词： Pathway feasibility analysis Protein cost Anaerobic metabolism Engineered pathways Metabolite concentrations

Recent advances and innovations in the design and fabrication of wearable flexible biosensors and human health monitoring systems based on conjugated polymers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bio-Design and Manufacturing 2024年第4期7卷 476-516页

作者： Vinh van Tran Viet-Duc Phung Daeho Lee Department of Mechanical Engineering Gachon UniversitySeongnam 13120Republic of Korea Institute of Fundamental and Applied Sciences Duy Tan UniversityHo Chi Minh City 700000Vietnam Faculty of Environmental and chemical Engineering Duy Tan UniversityDa Nang 550000Vietnam

Wearable biosensors have received great interest as patient-friendly diagnostic technologies because of their high flexibility and *** growing research and utilization of novel materials in designing wearable biosensors have accelerated the development of point-of-care sensing platforms and implantable biomedical devices in human health *** numerous potential materials,conjugated polymers(cPs)are emerging as ideal choices for constructing high-performance wearable biosensors because of their outstanding conductive and mechanical ***,cPs have been extensively incorporated into various wearable biosensors to monitor a range of target ***,fabricating highly reliable cP-based wearable biosensors for practical applications remains a significant challenge,necessitating novel developmental strategies for enhancing the viability of such ***,this review aims to provide consolidated scientific evidence by summarizing and evaluating recent studies focused on designing and fabricating cP-based wearable biosensors,thereby facilitating future *** the superior properties and benefits of cPs,this review aims to clarify their potential applicability within this ***,the fundamentals and main components of cP-based wearable biosensors and their sensing mechanisms are discussed in *** recent advancements in cP nanostructures and hybridizations for improved sensing performance,along with recent innovations in next-generation wearable biosensors are *** wearable biosensors have been—and will continue to be—an ideal platform for developing effective and user-friendly diagnostic technologies for human health monitoring.

关键词： conjugated polymers Wearable biosensors E-skin electronics Implantable biosensors conductive polymer hydrogels

Early prediction of major depression in chronic hepatitis c patients during peg-interferon α-2b treatment by assessment of vegetative-depressive symptoms after four weeks

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第43期13卷 5736-5740页

作者： Geert Robaeys Jozef De Bie Marieke c Wichers Liesbeth Bruckers Frederik Nevens Peter Michielsen Marc van Ranst Frank Buntinx Department of Gastroenterology and Hepatology Ziekenhuis Oost Limburg Genk Belgium Department of Psychiatry Ziekenhuis Oost Limburg Genk Belgium Department of Psychiatry and Neuro-psychiatry South Limburg Mental Health Research and Teaching Network EURON Maastricht University Maastricht The Netherlands center for Statistics Limburgs Universitair Centrum Diepenbeek Belgium Department of Hepatology Katholieke Universiteit Leuven Leuven Belgium Division of Gastroenterology and Hepatology University Hospital Antwerp Belgium Laboratory of clinical and Epidemiological Virology Department of Microbiology and Immunology Rega Institute and University Hospitals Katholieke Universiteit Leuven Leuven Belgium Department of General Practice Katholieke Universiteit Leuven Leuven Belgium and Department of General Practice Maastricht University The Netherlands

AIM: To study the predictive value of the vegetative- depressive symptoms of the Zung Depression Rating Scale for the occurrence of depression during treatment with peg-interferon α-2b of chronic hepatitis c (cHc) patients. METHODS: The predictive value of vegetative- depressive symptoms at 4 wk of treatment for the occurrence of a subsequent diagnosis of major depressive disorder (MDD) was studied in cHc patients infected after substance use in a prospective, multi- center treatment trial in Belgium. The presence of vegetative-depressive symptoms was assessed using the Zung Scale before and 4 wk after the start of antiviral treatment. RESULTS: Out of 49 eligible patients, 19 (39%) developed MDD. The area under the ROc curve of the vegetative Zung subscale was 0.73, P = 0.004. The sensitivity at a cut-point of > 15/35 was 95% (95% cI: 74-100). The positive predictive value equalled 44% (95% cI: 29-60). cONcLUSION: In this group of Belgian cHc patients infected after substance use, antiviral treatment caused a considerable risk of depression. Seven vegetativedepressive symptoms of the Zung scale at wk 4 of treatment predicted 95% of all emerging depressions, at a price of 56% false positive test results.

关键词： Interferons Hepatitis c chronic Substance-related disorders Depression Zung self rating scale Prognosis

The diagnostic and prognostic utility of the dual-task tandem gait test for pediatric concussion

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2021年第2期10卷 131-137页

作者： Katie A.van Deventer corrine N.Seehusen Gregory A.Walker Julie c.Wilson David R.Howell University of colorado School of Medicine AuroraCO 80045USA Sports Medicine center Children’s Hospital ColoradoAuroraCO 80045USA Department of Orthopedics University of Colorado School of MedicineAuroraCO 80045USA Department of Pediatrics University of Colorado School of MedicineAuroraCO 80045USA

Background:The tandem gait test has gained interest recently for assessment of concussion *** purpose of our study was to determine the prognostic and diagnostic use of the single-and dual-task tandem gait test,alongside other clinical measures,within 10 days of pediatric ***:We assessed 126 patients post-concussion(6.3§2.3 days post-injury,mean§SD)at a pediatric sports medicine clinic and compared them to 58 healthy controls(age:15.6§1.3 years;43%female).We also compared the 31 patients with concussion who developed persistent post-concussion symptoms(PPcS)(age=14.9§2.0 years;48%female)to the 81 patients with concussion who did not develop PPcS following the initial assessment(age:14.1§3.0 years;41%female).All subjects completed a test battery,and concussion patients were monitored until they experienced concussion-symptom *** test battery included tandem gait(single-task,dual-task(performing tandem gait while concurrently completing a cognitive test)conditions),modified Balance Error Scoring System(mBESS),and concussion symptom assessment(Health and Behavior Inventory).We defined PPcS as symptom resolution time>28 days post-concussion for the concussion *** outcomes included tandem gait time(single-and dual-task),dual-task cognitive accuracy,mBESS errors(single/double/tandem stances),and symptom ***:The concussion group completed the single-task(mean difference=9.1 s,95%confidential interval(95%cI):6.1-12.1)and dual-task(mean difference=12.7 s,95%cI:8.716.8)tandem gait test more slowly than the control *** to those who recovered within 28 days of concussion,the PPcS group had slower dual-task tandem gait test times(mean difference=7.9 s,95%cI:2.0-13.9),made more tandem-stance mBESS errors(mean difference=1.3 errors,95%cI:0.2-2.3),and reported more severe symptoms(mean difference=26.6 Health and Behavior Inventory rating,95%cI:21.1-32.6).conclusion:Worse dual-task tandem gait test time and mB

关键词： Adolescent Balance Mild traumatic brain injury Postural stability Prognosis

Tribology of enzymatically degraded cartilage mimicking early osteoarthritis

在线全文

学校读者我要写书评

暂无评论

Friction 2023年第9期11卷 1724-1740页

作者： Ke REN Miguel Alejandro REINA MAHEcHA Maike HÜBNER Zhiwei cUI Hans J.KAPER Hugo c.van DER VEEN Prashant K.SHARMA Department of Biomedical Engineering University Medical Centre GroningenUniversity of GroningenGroningen 9713AVthe Netherlands Department of Mechanical Engineering Eindhoven University of TechnologyEindhoven 5600MBthe Netherlands Department of Orthopedic Surgery University Medical Centre GroningenUniversity of GroningenGroningen 9700RBthe Netherlands

Healthy cartilage is a water-filled super lubricious *** type II provides it structural stability,and proteoglycans absorb water to keep the cartilage in a swollen condition,providing it the ability to creep and provide weeping ***(OA)is a degenerative and debilitating disorder of diarthrodial joints,where articular cartilage damage originates from enzymatic degradation and mechanical damage(wear).The objective of this research is to observe the level of cartilage damage present in knee arthroplasty patients and to understand the friction and creep behavior of enzymatically degraded bovine cartilage in ***(Lat)and medial(Med)condylar cartilages from OA patients undergoing total knee arthroplasty showed signs of enzymatic degradation and mechanical *** cartilages were exposed to collagenase III and chondroitinase ABc to degrade collagen and proteoglycans,*** loss of proteoglycans or collagen network and morphological changes were observed through histology and the atomic force microscope(AFM),respectively.A significant effect on creep due to enzymatic treatment was not *** the enzymatic treatment was found to significantly decrease the coefficient of friction(cOF)at 4 N,while higher cOF was shown from chondroitinase ABc degraded cartilage at 40 *** III treatment leads to the release of intact proteoglycans at the sliding interface,while chondroitinase ABc treatment leads to the loss of chondroitin sulfate(cS)from the *** ABc-digested bovine cartilage mimicked patient samples the best because of the similar distributions of proteoglycans,collagen network,and friction behavior.

关键词： lubrication cartilage boundary lubrication osteoarthritis(OA)

Why static bound-state calculations of tetraquarks should be met with scepticism

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

chinese Physics c 2017年第5期41卷 49-53页

作者： George Rupp Eef van Beveren centro de Fisica e Engenharia de Materiais Avancados Instituto Superior Tecnico Universidade de Lisboa P-1049-001 Lisboa Portugal centro de Fisica da Uc Departamento de Fisica Universidade de Coimbra P-3004-516 Coimbra Portugal

Recent experimental signals have led to a revival of tetraquarks,the hypothetical q~2q~2 hadronic states proposed by Jaffe in 1976 to explain the light scalar *** structures with exotic quantum numbers have indeed been observed recently,though a controversy persists as to whether these are true resonances and not merely kinematical threshold enhancements,or otherwise states not of a true q~2q~2 ***,puzzling non-exotic mesons are also often claimed to have a tetraquark ***,the corresponding model calculations are practically always carried out in pure and static bound-state approaches,ignoring completely the coupling to asymptotic two-meson states and unitarity,especially the dynamical effects *** this short paper we argue that these static predictions of real tetraquark masses are highly unreliable and provide little evidence of the very existence of such states.

关键词： meson scalar quark dynamical asymptotic otherwise argue merely decay exotic