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molecular phylogeny, morphology, pigment chemistry and ecology in Hygrophoraceae (Agaricales)

Fungal Diversity 2014年第1期64卷 1-99页

作者： D.Jean Lodge mahajabeen Padamsee P.Brandon matheny m.Catherine Aime Sharon A.Cantrell David Boertmann Alexander Kovalenko Alfredo Vizzini bryn t.m.dentinger Paul m.Kirk A.martyn Ainsworth Jean-marc moncalvo Rytas Vilgalys Ellen Larsson Robert Lücking Gareth W.Griffith matthew E.Smith Lorelei L.Norvell Dennis E.Desjardin Scott A.Redhead Clark L.Ovrebo Edgar B.Lickey Enrico Ercole Karen W.Hughes Régis Courtecuisse Anthony Young manfred Binder Andrew m.minnis Daniel L.Lindner Beatriz Ortiz-Santana John Haight thomas Læssøe timothy J.Baroni József Geml tsutomu Hattori Center for Forest mycology Research Northern Research StationUSDA-Forest ServiceLuquilloPR 00773-1377USA Systematics team Landcare Research231 Morrin RoadAuckland 1072New Zealand Ecology&Evolutionary Biology University of TennesseeKnoxvilleTN 37996-1610USA Department of Botany&Plant Pathology Purdue UniversityWest LafayetteIN 47907USA Science and technology Universidad del TuraboGuraboPR 00778-3030USA Department of Bioscience Aarhus UniversityFrederiksborgvej 399DK-4000 RoskildeDenmark Komarov Botanical Institute of the Russian Academy of Sciences 2 Prof.Popov Str.St.Petersburg 197376Russia Dipartimento di Scienze della Vita e Biologia dei Sistemi Universitàdi TorinoViale P.A.Mattioli 2510125 TorinoItaly mycology Royal Botanic GardensKewRichmondSurrey TW93DSUK Royal Ontario museum 100 Queen’s ParkTorontoONCanada Biology Department Duke UniversityDurhamNC 27708-0338USA Biological&Environmental Sciences University of GothenburgGöteborgSweden Science&Eduaction Integrative Research&Collections(Botany)The Field Museum of Natural History1400 Lake Shore Dr.ChicagoIL 60605-2496USA University of Wales AberystwythCeredigion SY233DDUK Department of Plant Pathology University of FloridaGainesvilleFL 32611-0680USA Pacific Northwest mycology Service 6720 NW Skyline BvdPortlandOR 97229-1309USA Department of Biology San Francisco State University1600 Holloway Ave.San FranciscoCA 94132USA National mycological Herbarium(DAOm) Science and Technology BranchAgriculture&Agri-FoodsCanada960 Carling AvenueOttawaOntarioCanada ONT K1A 0C6 Department of Biology University of Central OklahomaEdmondOK 73034USA Department of Biology Bridgewater College402 E.College St.BridgewaterVA 22812USA Department of Botany Faculty of Pharmaceutical&Biological SciencesLilleFrance 100 Langton Road BlackbuttQld 4306Australia CBS Fungal Biodiversity Centre Evolutionary PhytopathologyInst.of the Royal Netherlands Academy of Arts&Sciences(KNAW)Uppsalalaan 83584 CT UtrechtThe Netherlands

molecular phylogenies using 1–4 gene regions and information on ecology,morphology and pigment chemistry were used in a partial revision of the agaric family *** phylogenetically supported genera we recognize here in the Hygrophoraceae based on these and previous analyses are:Acantholichen,Ampulloclitocybe,Arrhenia,Cantharellula,Cantharocybe,Chromosera,Chrysomphalina,Cora,Corella,Cuphophyllus,Cyphellostereum,Dictyonema,Eonema,Gliophorus,Haasiella,Humidicutis,Hygroaster,Hygrocybe,Hygrophorus,Lichenomphalia,Neohygrocybe,Porpolomopsis and Pseudoarmillariella.A new genus that is sister to Chromosera is described as *** were made at the ranks of subfamily,tribe,genus,subgenus,section and *** present three new subfamilies,eight tribes(five new),eight subgenera(one new,one new combination and one ***.),26 sections(five new and three new combinations and two ***.)and 14 subsections(two new,two ***.).Species of Chromosera,Gliophorus,Humidicutis,and Neohygrocybe are often treated within the genus Hygrocybe;we therefore provide valid names in both classification *** used a minimalist approach in transferring genera and creating new names and ***,we retain in the Hygrophoraceae the basal cuphophylloid grade comprising the genera Cuphophyllus,Ampulloclitocybe andCantharocybe,despite weak phylogenetic *** include Aeruginospora and Semiomphalina in Hygrophoraceae based on morphology though molecular data are *** lower hygrophoroid clade is basal to Hygrophoraceae s.s.,comprising the genera Aphroditeola,macrotyphula,Phyllotopsis,Pleurocybella,Sarcomyxa,tricholomopsis and typhula.

关键词： Hygrophoraceae Fungi Revisionary systematics Nomenclatural revision Phylogenetics Pigment chemistry Lamellar trama construction Hymenial morphology Ecology

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taming the beast:a revised classification of Cortinariaceae based on genomic data

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Fungal Diversity 2022年第1期112卷 89-170页

作者： Kare Liimatainen Jan t.Kim Lisa Pokorny Paul m.Kirk bryn dentinger tuula Niskanen Jodrell Laboratory Royal Botanic GardensKewSurrey TW93ABUK Department of Computer Science School of PhysicsEngineering and Computer ScienceUniversity of HertfordshireHatfieldHertfordshire AL109ABUK Institut Botànic de Barcelona(IBB CSIC-Ajuntament de Barcelona)08038 BarcelonaCataloniaSpain Biodiversity Informatics and Spatial Analysis Jodrell LaboratoryRoyal Botanic GardensKewSurrey TW93ABUK Natural History museum of Utah and School of Biological Sciences University of UtahSalt Lake CityUTUSA Botanical museum University of HelsinkiP.O.Box 700014 HelsinkiFinland

Family Cortinariaceae currently includes only one genus,Cortinarius,which is the largest Agaricales genus,with thousands of species *** species are important ectomycorrhizal fungi and form associations with many vascular plant gen-era from tropicals to arctic *** Cortinarius contains a lot of morphological variation,and its complexity has led many taxonomists to specialize in particular on infrageneric *** previous attempts to divide Cortinarius have been shown to be unnatural and the phylogenetic studies done to date have not been able to resolve the higher-level classification of the group above section *** approaches have revolutionized our view on fungal relationships and provide a way to tackle difficult *** used both targeted capture sequencing and shallow whole genome sequencing to produce data and to perform phylogenomic analyses of 75 single-copy genes from 19 *** addition,a wider 5-locus analysis of 245 species,from the Northern and Southern Hemispheres,was also *** on our results,a classification of the family Cortinariaceae into ten genera-Cortinarius,Phlegmacium,thaxterogaster,Calonarius,Aureonarius,Cystinarius,Volvanarius,Hygronarius,mystinarius,and Austrocortinarius-is *** genera,10 subgenera,and four sec-tions are described as new to science and five subgenera are introduced as new combinations in a new *** addition,41 section names and 514 species names are combined in new genera and four lecto-and epitypes *** position of Stephanopus in suborder Agaricineae remains to be *** capture sequencing is used for the first time in fungal taxonomy in *** provides a cost-efficient way to produce-omics data in species-rich ***-omics data was produced from fungarium specimens up to 21 years old,demonstrating the value of museum specimens in the study of the fungal tree of *** study is the first family revision in Agaricales based on genomi

关键词： Agaricales Fungariomics Fungi HybPiper museomics targeted capture sequencing Whole genome sequencing

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Fungal diversity notes 253-366:taxonomic and phylogenetic contributions to fungal taxa

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Fungal Diversity 2016年第3期78卷 1-237页

作者： Guo Jie Li Kevin D.Hyde Rui Lin Zhao Sinang Hongsanan Faten Awad Abdel-Aziz mohamed A.Abdel-Wahab Pablo Alvarado Genivaldo Alves-Silva Joseph F.Ammirati Hiran A.Ariyawansa Abhishek Baghela Ali Hassan Bahkali michael Beug D.Jayarama Bhat Dimitar Bojantchev thitiya Boonpratuang timur S.Bulgakov Erio Camporesi marcela CBoro Oldriska Ceska Dyutiparna Chakraborty Jia Jia Chen K.W.thilini Chethana Putarak Chomnunti Giovanni Consiglio Bao Kai Cui Dong Qin Dai Yu Cheng Dai Dinushani A.Daranagama Kanad Das monika C.Dayarathne Eske De Crop Rafael J.V.De Oliveira Carlos Alberto Fragoso de Souza JoséIde Souza bryn t.m.dentinger Asha J.Dissanayake mingkwan Doilom E.Ricardo Drechsler-Santos masoomeh Ghobad-Nejhad Sean P.Gilmore Aristóteles Góes-Neto michałGorczak Charles H.Haitjema Kalani Kanchana Hapuarachchi Akira Hashimoto mao Qiang He John K.Henske Kazuyuki Hirayama maria J.Iribarren Subashini C.Jayasiri Ruvishika S.Jayawardena Sun Jeong Jeon Gustavo H.Jerônimo Ana L.Jesus E.B.Gareth Jones Ji Chuan Kang Samantha C.Karunarathna Paul m.Kirk Sirinapa Konta Eric Kuhnert Ewald Langer Haeng Sub Lee Hyang Burm Lee Wen Jing Li Xing Hong Li Kare Liimatainen Diogo Xavier Lima Chuan Gen Lin Jian Kui Liu Xings Zhong Liu Zuo Yi Liu J.Jennifer Luangsa-ard Robert Lücking H.thorsten Lumbsch Saisamorn Lumyong Eduardo m.Leaño Agostina V.marano misato matsumura Eric H.C.mcKenzie Suchada mongkolsamrit Peter E.mortimer thi thuong thuong Nguyen tuula Niskanen Chada Norphanphoun michelle A.O’malley Sittiporn Parnmen Julia Pawłowska Rekhani H.Perera Rungtiwa Phookamsak Chayanard Phukhamsakda Carmen L.A.Pires-Zottarelli Olivier Raspé mateus A.Reck Sarah C.O.Rocha AndréL.C.m.Ade Santiago Indunil C.Senanayake Ledo Setti Qiu Ju Shang Sanjay K.Singh Esteban B.Sir Kevin V.Solomon Jie Song Prasert Srikitikulchai marc Stadler Satinee Suetrong Hayato takahashi takumasa takahashi Kazuaki tanaka Li Ping tang Kasun m.thambugala Donnaya thanakitpipattana michael K.theodorou Benjarong thongbai tuksaporn thummarukcharoen Qing tian Saowaluck tibpromma Annemieke State Key Laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesNo 31st West Beichen RoadChaoyang DistrictBeijing 100101People’s Republic of China Centre of Excellence in Fungal Research and School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of ScienceKunming 650201YunnanPeople’s Republic of China Department of Botany and microbiology College of ScienceKing Saud UniversityP.O.Box:2455Riyadh 1145Saudi Arabia Department of Botany and microbiology Faculty of ScienceSohag UniversitySohag 82524Egypt ALVALAB C/La Rochela nº47E-39012 SantanderSpain micolab Programa de Pós-Graduação em Biologia de FungosAlgas e PlantasDepartamento de BotânicaUniversidade Federal de Santa CatarinaCampus Universitário TrindadeCEP:88040-900 FlorianópolisSanta CatarinaBrazil Department of Biology University of WashingtonBox 351800SeattleWA 98195-1800USA Guizhou Key Laboratory of Agricultural Biotechnology Guizhou Academy of Agricultural SciencesGuiyang 550006GuizhouPeople’s Republic of China National Fungal Culture Collection of India(NFCCI) Biodiversity and Palaeobiology GroupMACS’Agharkar Research InstituteG.G.Agarkar RoadPune 411004India PO Box 116 HusumWA 98623-0116USA Formerly Department of BotanyGoa UniversityGoaIndia No.128/1-J Azad Housing SocietyCurcaP.O.Goa Velha 403108India *** 345 Shipwatch LaneHerculesCA 94547USA microbe Interaction Laboratory(BmIt) BIOTECNational Science and Technology Development Agency(NSTDA)113 Thailand Science ParkThanon PhahonyothinTombon Khlong NuengAmphoeKhlong LuangPathum Thani 12120Thailand Academy of Biology and Biotechnology Southern Federal UniversityRostov-on-Don 344090Rostov regionRussia A.m.B.Gruppo micologico Forlivese BAntonio“Cicognani” Via Roma Forlì Italy 18 A.M.B.Circolo Micologico“Giovanni Carini”C.P.314BresciaItaly Societàper gli Studi Naturalistici della Romagna C.P.144Bagnacavallo

Notes on 113 fungal taxa are compiled in this paper,including 11 new genera,89 new species,one new subspecies,three new combinations and seven reference *** geographic and taxonomic range of fungal taxa are *** the Ascomycota the new genera Angustospora(testudinaceae),Camporesia(Xylariaceae),Clematidis,Crassiparies(Pleosporales genera incertae sedis),Farasanispora,Longiostiolum(Pleosporales genera incertae sedis),multilocularia(Parabambusicolaceae),Neophaeocryptopus(Dothideaceae),Parameliola(Pleosporales genera incertae sedis),and towyspora(Lentitheciaceae)are *** introduced species are Angustospora nilensis,Aniptodera aquibella,Annulohypoxylon albidiscum,Astrocystis thailandica,Camporesia sambuci,Clematidis italica,Colletotrichum menispermi,***,Comoclathris pimpinellae,Crassiparies quadrisporus,Cytospora salicicola,Diatrype thailandica,Dothiorella rhamni,Durotheca macrostroma,Farasanispora avicenniae,Halorosellinia rhizophorae,Humicola koreana,Hypoxylon lilloi,Kirschsteiniothelia tectonae,Lindgomyces okinawaensis,Longiostiolum tectonae,Lophiostoma pseudoarmatisporum,moelleriella phukhiaoensis,***,mucoharknessia anthoxanthi,multilocularia bambusae,multiseptospora thysanolaenae,Neophaeocryptopus cytisi,Ocellularia arachchigei,***,Ochronectria thailandica,Ophiocordyceps karstii,Parameliola acaciae,***,Parastagonospora cumpignensis,Pseudodidymosphaeria phlei,Polyplosphaeria thailandica,Pseudolachnella brevifusiformis,Psiloglonium macrosporum,Rhabdodiscus albodenticulatus,Rosellinia chiangmaiensis,Saccothecium rubi,Seimatosporium pseudocornii,***,Sigarispora ononidis and towyspora *** combinations are provided for Eutiarosporella dactylidis(sexual morph described and illus trated)and Pseudocamarosporium ***,illustrations and/or reference specimens are designated for Aposphaeria corallinolutea,Cryptovalsa ampelina,Dothiorella vidmadera,Ophiocordyceps formosana,P

关键词： Ascomycota Basidiomycota Neocallimastigomycota Oomycota.Zygomycota Phylogeny taxonomy New genus New species

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Review of laser diagnostics at the Vulcan laser facility

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High Power Laser Science and Engineering 2015年第3期3卷 3-12页

作者： Ian musgrave marco Galimberti Alexis Boyle Cristina Hernandez-Gomez Andrew Kidd bryn Parry Dave Pepler trevor Winstone John Collier Central Laser Facility Science and Technology Facilities CouncilRAL

In this paper we review the provision of the laser diagnostics that are installed on the Vulcan laser facility. We will present strategies for dealing with the energy of high energy systems and with ways of handling the beam sizes of the lasers. We present data captured during typical experimental campaigns to demonstrate their reliability and variation in shot to shot values.

关键词： calorimeter high power laser laser diagnostics pulse energy

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multidimensional synthetic chiral-tube lattices via nonlinear frequency conversion

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Light(Science & Applications) 2020年第1期9卷 803-812页

作者： Kai Wang bryn A.Bell Alexander S.Solntsev Dragomir N.Neshev Benjamin J.Eggleton Andrey A.Sukhorukov Nonlinear Physics Centre Research School of PhysicsThe Australian National UniversityCanberraACT 2601Australia Institute of Photonics and Optical Science(IPOS) School of PhysicsUniversity of SydneySydneyNSW 2006Australia Department of Physics QOLSImperial College LondonLondon SW72AZUK School of mathematical and Physical Sciences University of Technology SydneyUltimoNSW 2007Australia Ginzton Laboratory Stanford UniversityStanfordCA 94305USA

Geometrical dimensionality plays a fundamentally important role in the topological effects arising in discrete *** direct experiments are limited by three spatial dimensions,the research topic of synthetic dimensions implemented by the frequency degree of freedom in photonics is rapidly *** manipulation of light in these artificial lattices is typically realized through electro-optic modulation;yet,their operating bandwidth imposes practical constraints on the range of interactions between different frequency *** we propose and experimentally realize all-optical synthetic dimensions involving specially tailored simultaneous short-and long-range interactions between discrete spectral lines mediated by frequency conversion in a nonlinear *** realize triangular chiral-tube lattices in three-dimensional space and explore their four-dimensional *** implement a synthetic gauge field with nonzero magnetic flux and observe the associated multidimensional dynamics of frequency combs,all within one physical spatial *** anticipate that our method will provide a new means for the fundamental study of high-dimensional physics and act as an important step towards using topological effects in optical devices operating in the time and frequency domains.

关键词： dimensions chiral nonlinear

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Femoro Patella Vialla patellofemoral arthroplasty: An independent assessment of outcomes at minimum 2-year follow-up

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World Journal of Orthopedics 2016年第8期7卷 487-493页

作者： mansur Halai Andrew Ker Iain Anthony Graeme Holt bryn Jones mark Blyth Department of trauma and Orthopaedics Glasgow Royal Infirmary Department of trauma and Orthopaedics University Hospital Crosshouse

AIm: to determine outcomes using the Femoro-Patella Vialla(FPV) arthroplasty and if there is an ideal patient for this implant. mEtHODS: A total of 41 FPV patellofemoral joint replacements were performed in 31 patients(22 females, 9 males, mean age 65 years). mean followup was 3.2 years(minimum 2 years). Radiographs were reviewed preoperatively and postoperatively. We assessed whether gender, age, previous surgery, patella atla or trochlear dysplasia influenced patient satisfaction or patient functional ***: the median Oxford Knee Score was 40 and the median melbourne Patellofemoral Score was 21 postoperatively. Seventy-six percent of patients were satisfied, 10% unsure and 14% dissatisfied postoperatively. there was no radiological progression of tibiofemoral joint arthritis, using the Ahlback grading,in any patient. One patient, who was diagnosed with rheumatoid arthritis postoperatively, underwent revision to total knee replacement. there were no intraoperative lateral releases and no implant failures. Gender, age, the presence of trochlear dysplasia, patella alta or bilateral surgery did not influence patient outcome. Previous surgery did not correlate with outcome. CONCLUSION: In contrast to the current literature, the FPV shows promising early results. However, we cannot identify a subgroup of patients with superior outcomes.

关键词： Patellofemoral Arthritis Femoro-Patella Vialla Outcomes Arthroplasty

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Spatial transferability of Vegetation types in Distribution models Based on Sample Surveys from an Alpine Region

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Journal of Geographic Information System 2018年第1期10卷 111-141页

作者： Linda Aune-Lundberg Anders bryn Division of Survey and Statistics Norwegian Institute of Bioeconomy Research Troms&#248 Norway Division of Survey and Statistics Norwegian Institute of Bioeconomy Research &#197 s Norway Natural History museum University of Oslo Oslo Norway

Vegetation mapping using field surveys is expensive. Distribution modelling, based on sample surveys, might overcome this challenge. We tested if models trained from sample surveys could be used to predict the distribution of vegetation types in neighbourhood areas, and how reliable the spatial transferability was. We also tested whether we should use ecological dissimilarity or spatial distance to foresee modelling performance. maximum entropy models were run for three vegetation types based on a vegetation map within a mountain range. Environmental variables were selected backwards, model complexity was kept low. the models are based on points from a small part of each study site, transferred into the entire sites, and then tested for performance. Environmental distance was tested using principle component analysis. All models had high uncorrected AUC values. the ability to predict presences correctly was low. the ability to predict absences correctly was high. the ability to transfer the distribution model depended on environmental distance, not spatial distance.

关键词： Area Frame Survey Ecological Distance GIS Independent Evaluation Data maximum Entropy modelling Vegetation mapping

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A mini-Review and Perspective on Current Best Practice and Emerging Industry 4.0 methods for Risk Reduction in Advanced Composites manufacturing

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Open Journal of Composite materials 2021年第2期11卷 31-45页

作者： bryn Crawford Hamid Khayyam Abbas S. milani Composites Research Network School of Engineering University of British Columbia Kelowna Canada School of Engineering RMIT University Melbourne Melbourne Australia

the manufacturing of composite structures is a highly complex task with inevitable risks, particularly associated with aleatoric and epistemic uncertainty of both the materials and processes, as well as the need for in-situ decision-making to mitigate defects during manufacturing. In the context of aerospace composites production in particular, there is a heightened impetus to address and reduce this risk. Current qualification and substantiation frameworks within the aerospace industry define tractable methods for risk reduction. In parallel, Industry 4.0 is an emerging set of technologies and tools that can enable better decision-making towards risk reduction, supported by data-driven models. It offers new paradigms for manufacturers, by virtue of enabling in-situ decisions for optimizing the process as a dynamic system. However, the static nature of current (pre-Industry 4.0) best-practice frameworks may be viewed as at odds with this emerging novel approach. In addition, many of the predictive tools leveraged in an Industry 4.0 system are black-box in nature, which presents other concerns of tractability, interpretability and ultimately risk. this article presents a perspective on the current state-of-the-art in the aerospace composites industry focusing on risk reduction in the autoclave processing, as an example system, while reviewing current trends and needs towards a Composites 4.0 future.

关键词： Advanced Composites manufacturing Quality Control Uncertainty Knowledge Engineering Industry 4.0 machine Learning Limited Data modeling

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Detection of Antibody in Dogs with Blastomycosis Using <i>Blastomyces dermatitidis</i>Yeast Phase Lysate Antigens

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Open Journal of Veterinary medicine 2017年第9期7卷 111-119页

作者： bryn C. Kennell Gene m. Scalarone Lilliana Hoyos-Carvajal moytri RoyChowdhury Department of Biological Sciences Idaho State University 650 Memorial Dr. Gale Life Sciences Building Pocatello ID USA Universidad Nacional de Colombia Sede Medellín Colombia

the objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin;85, soil Georgia, AtCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. the mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. the mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418;Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452;85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150;85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. this study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.

关键词： Blastomyces Dermatitidis Yeast Phase Lysate Antigen Blastomycosis

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Spatial distribution and network morphology of epicardial,endocardial,interstitial,and Purkinje cell-associated elastin fibers in porcine left ventricle

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Bioactive materials 2023年第1期19卷 348-359页

作者： Xiaodan Shi Song Zhang Yue Liu bryn Brazile Jim Cooley J.Ryan Butler Sara R.mcmahan Karla L.Perez Jiazhu Xu timothy Eastep Kytai t.Nguyen Pietro Bajona matthias Peltz Huajian Gao Yi Hong Jun Liao Department of Bioengineering University of Texas at ArlingtonArlingtonTX76010USA College of Engineering and College of Veterinary medicine Mississippi State UniversityMississippi StateMS39762USA School of Engineering Brown UniversityProvidenceRI02912USA College of Engineering Nanyang Technical University308232Singapore Department of Cardiovascular and thoracic Surgery University of Texas Southwestern Medical CenterDallasTX75390USA Allegheny Health Network-Drexel University College of medicine PittsburghPA15212USA

Cardiac extracellular matrices(ECm)play crucial functional roles in cardiac *** studies have mainly focused on collagen,the major structural ECm in heart *** role of elastin in cardiac mechanics,however,is poorly *** this study,we investigated the spatial distribution and microstructural morphologies of cardiac elastin in porcine left *** demonstrated that the epicardial elastin network had location-and depth-dependency,and the overall epicardial elastin fiber mapping showed certain correlation with the helical heart muscle fiber *** compared to the epicardial layer,the endocardial layer was thicker and has a higher elastin-collagen ratio and a denser elastin fiber network;moreover,the endocardial elastin fibers were finer and more wavy than the epicardial elastin fibers,all suggesting various interface *** myocardial interstitial elastin fibers co-exist with the perimysial collagen to bind the cardiomyocyte bundles;some of the interstitial elastin fibers showed a locally aligned,hinge-like structure to connect the adjacent cardiomyocyte *** collagen-elastin combination reflects an optimal design in which the collagen provides mechanical strength and elastin fibers facilitate recoiling during ***,cardiac elastin fibers,along with collagen network,closely associated with the Purkinje cells,indicating that this ECm association could be essential in organizing cardiac Purkinje cells into“fibrous”and“branching”morphologies and serving as a protective feature when Purkinje fibers experience large deformations in *** short,our observations provide a structural basis for future in-depth biomechanical investigations and biomimicking of this long-overlooked cardiac ECm component.

关键词： Heart ECm Epicardial elastin Endocardial elastin Interstitial elastin Purkinje-cell associated elastin

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