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Comparison of point detection and area detection for point-scanning structured illumination microscopy

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创新光学健康科学杂志(英文) 2023年第4期16卷 134-149页

作者： Wenshuai Wu Jiajie Chen Meiting Wang Lei Wang Xiaomin Zheng Jia Li Junle Qu bruce zhi gao Yonghong Shao College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060GuangdongP.R.China Department of Bioengineering and COMSET Clemson University ClemsonSC 29634 USA

Structured illumination microscopy(SIM)is suitable for biological samples because of its relatively low-peak illumination intensity requirement and high imaging *** system resolution is affected by two typical detection modes:Point detection and area ***,a systematic analysis of the imaging performance of the different detection modes of the system has rarely been *** this study,we compared laser point scanning point detection(PS-PD)and point scanning area detection(PS-AD)imaging in nonconfocal microscopy through theoretical analysis and simulated *** results revealed that the imaging resolutions of PSPD and PS-AD depend on excitation and emission point spread functions(PSFs),***,we combined the second harmonic generation(SHG)of point detection(P-SHG)and area detection(A-SHG)with SIM to realize a nonlinear SIM-imaging technique that improves the imaging ***,we analytically and experimentally compared the nonlinear SIM performance of P-SHG with that of A-SHG.

关键词： Super-resolution structured illumination microscopy second harmonic generation

Low-temperature optothermal nanotweezers

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Nano Research 2023年第5期16卷 7710-7715页

作者： Jianxing Zhou Xiaoqi Dai Yuhang Peng Yili Zhong Ho-Pui Ho Yonghong Shao bruce zhi gao Junle Qu Jiajie Chen State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical Engineering The Chinese University of Hong KongHong Kong 999077China Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA

Optical tweezers that rely on laser irradiation to capture and manipulate nanoparticles have provided powerful tools for biological and biochemistry ***,the existence of optical diffraction-limit and the thermal damage caused by high laser power hinder the wider application of optical tweezers in the biological *** the past decade,the emergence of optothermal tweezers has solved the above problems to a certain extent,while the auxiliary agents used in optothermal tweezers still limit their ***,we report a kind of nanotweezers based on the sign transformation of the thermophoresis coefficient of colloidal particles in low-temperature *** a self-made microfluidic refrigerator to reduce the ambient temperature to around 0℃in the microfluidic cell,we can control a single nanoparticle at lower laser power without adding additional agent solute in the *** novel optical tweezering scheme has provided a new path for the manipulation of inorganic nanoparticles as well as biological particles.

关键词： optothermal tweezers optical manipulation microfluidic device thermophoresis thermo-osmotic flow

CRISPR-powered optothermal nanotweezers:Diverse bio-nanoparticle manipulation and single nucleotide identification

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Light(Science & Applications) 2023年第12期12卷 2644-2654页

作者： Jiajie Chen zhi Chen Changle Meng Jianxing Zhou Yuhang Peng Xiaoqi Dai Jingfeng Li Yili Zhong Xiaolin Chen Wu Yuan Ho-Pui Ho bruce zhi gao Junle Qu Xueji Zhang Han Zhang Yonghong Shao State Key Laboratory of Radio Frequency Heterogeneous Integration Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical Engineering The Chinese University of Hong KongShatinHong KongChina Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA School of Biomedical Engineering Shenzhen UniversityShenzhenGuangdong 518060China

Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of ***,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely ***,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA ***,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide ***,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable *** its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.

关键词： manipulation thermal identification

Improvement in Resolution of Multiphoton Scanning Structured Illumination Microscopy via Harmonics

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Engineering 2022年第9期16卷 65-72页

作者： Lei Wang Xiaomin Zheng Jie Zhou Meiting Wang Jiajie Chen Youjun Zeng Gaixia Xu Ying Wang Haixia Qiu Yonghong Shao Junle Qu bruce zhi gao Ying Gu College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen UniversityShenzhen 518060China Department of Laser Medicine First Medical Center of PLA General HospitalBeijing 100853China Department of Bioengineering and COMSET Clemson UniversityClemsonSC 29634USA

We describe a multiphoton(mP)-structured illumination microscopy(SIM)technique,which demonstrates substantial improvement in image resolution compared with linear SIM due to the nonlinear response of *** nonlinear response is caused by the effect of nonsinusoidal structured illumination created by scanning a sinusoidally modulated illumination to excite an mP fluorescence *** harmonics of the structured fluorescence illumination are utilised to improve *** present an mP-SIM theory for reconstructing the super-resolution image of the ***,the resolution of our m P-SIM is unlimited if all the high-order harmonics of the nonlinear response of fluorescence are ***,we demonstrate an 86 nm lateral resolution for two-photon(2P)-SIM and a 72 nm lateral resolution for second-harmonic-generation(SHG)-*** further demonstrate their application by imaging cells stained with F-actin and collagen fibres in mouse-tail *** method can be directly used in commercial mP microscopes and requires no specific fluorophores or high-intensity laser.

关键词： Super-resolution microscopy Structured illumination microscopy Multiphoton-structured illumination microscopy(SIM) SIM Second-harmonic-generation(SHG)-SIM

Scanless multitarget-matching multiphoton excitation fluorescence microscopy

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Journal of Innovative Optical Health Sciences 2018年第2期11卷 1-7页

作者： Junpeng Qiu Lei Wang bruce zhi gao Junle Qu Yonghong Shao College of Optoelectronics Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060P.R.China Department of Bioengineering and COMSET Clemson University ClemsonSC 29634USA

Using the combination of a refective blazed grating and a reflective phase-only difractive spatiallight modulator(SLM),scanless multitarget-matching multiphoton excitation fuorescence ***(SMTM-MP M)was *** SLM shaped an incoming mode-locked,near-infraredTi:sapphire laser beam into an excitation pattern with addressable shapes and sizes that matchedthe samples of interest in the field of *** and spatial focusing were simultaneouslyrealized by combining an objective lens and a blazed *** fluorescence signal fromilluminated areas was recorded by a two-dimensional sCMOS *** with a conventional temporal focusing multiphoton microscope,our microscope achieved effective use of thelaser power and decreased photodamage with higher axial resolution.

关键词： Multitarget-matching multiphoton microscopy SLM temporal focusing

The effects of cool materials,façade orientation,and morphological parameters on energy consumption at the residential neighborhood scale

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Building Simulation 2024年第4期17卷 525-542页

作者： Fusuo Xu Dongdong Tian zhi gao Jianshun Jensen Zhang Department of Mechanical and Aerospace Engineering Syracuse UniversitySyracuseNY13210USA School of Architecture and Urban Planning Nanjing University22 Hankou RoadNanjingJiangsu Province210093China

Building surface cool materials are novel materials that can reduce urban heat island intensity and decrease building energy *** study investigated the impact of radiative properties of materials,façade orientation,and morphological parameters on energy consumption in six typical residential neighborhoods in Nanjing,*** neighborhood energy consumption of 16 application schemes considering the façade orientation factor is compared to determine the best energy-saving *** and annual energy-saving rates,savings in electricity costs,and the price ceiling for materials per unit area are *** results show that for low-rise buildings,using cool materials only on the roof can reduce the annual energy consumption by 1%.When cool or super cool materials are also used on the building façade,the annual energy saving rate can be up to 3.4%and 4.3%,*** cool materials on the south façade of buildings is not recommended due to significant heat loss in *** savings in electricity costs and the price ceiling for materials per unit area,the price of cool and super cool materials should be less than 3.0 and 3.7 RMB/m2,respectively,assuming a lifespan of eight years in Nanjing.

关键词： cool materials building energy saving neighborhood morphology façade orientation

Spatial covariant gravity with two degrees of freedom in the presence of an auxiliary scalar field:Perturbation analysis

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Chinese Physics C 2024年第8期48卷 198-212页

作者： zhi-Chao Wang Xian gao School of Physics and Astronomy Sun Yat-sen UniversityZhuhai 519082China

We investigate a class of gravity theories respecting only spatial covariance,termed spatially covariant gravity,in the presence of an auxiliary scalar *** examine the conditions on the Lagrangian required to eliminate scalar degrees of freedom,allowing only two tensorial degrees of freedom to *** of strict constraint analysis,in this paper,we employ the perturbation method and focus on the necessary conditions to evade the scalar mode at the linear order in perturbations around a cosmological *** with a general action and solving the auxiliary perturbation variables in terms of a would-be dynamical scalar mode,we derive the condition to remove its kinetic term,thus ensuring that no scalar mode *** an application of the general condition,we study a polynomial-type Lagrangian as a concrete example,in which all monomials are spatially covariant scalars containing two *** find that the auxiliary scalar field is essential,and new terms in the Lagrangian are *** analysis provides insights into constructing gravity theories with two degrees of freedom in the extended framework of spatially covariant gravity.

关键词： modified gravity cosmological perturbation theory gravitational theory

Precise Asymptotics in Limit Theorems for a Supercritical Branching Process with Immigration in a Random Environment

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Acta Mathematica Sinica,English Series 2024年第8期40卷 1850-1874页

作者： Chun Mao HUANG Rui ZHANG zhi Qiang gao Department of Mathematics Harbin Institute of Technology(Weihai)Weihai 264209P.R.China School of Mathematical Sciences Beijing Normal UniversityBeijing 100875P.R.China

Let(Zn)be a supercritical branching process with immigration in an independent and identically distributed random *** necessary moment conditions,we show the exact convergence rate in the central limit theorem on log Zn and establish the corresponding local limit theorem by using the moments of the natural submartingale and the convergence rates of its *** similar approach and with the help of a change of measure,we also present the so-called integrolocal theorem and integral large deviation theorem to characterize the precise asymptotics of the upper large deviations.

关键词： Branching process with immigration random environment central limit theorem large deviation

Microfabrication,surface modification,and laser guidance techniques to create a neuron biochip

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Optoelectronics Letters 2008年第5期4卷 387-390页

作者： Russell Kirk Pirlo Xiang Peng Xiaocong Yuan bruce zhi gao Department of Bioengineering and Center for Optical Materials Science and Engineering Technologies Clemson University Clemson SC 29634 USA Institute of Optoelectronics Shenzhen University Shenzhen 518060 China School of Electrical ＆ Electronic Engineering Nanyang Technological University Nanyang Avenue Singapore 639798

In this report we illustrate our application of soft lithography-based microfabrication,surface modification,and our unique laser cell-patterning system toward the creation of neuron biochips. We deposited individual forebrain neurons from Day 7 embryonic chicks into two rows of eight in a silicon microstructure aligned over a microelectrode array (MEA). The polydimethylsiloxane (PDMS) membrane with microstructures to confine cells and guide network connectivity was aligned to the electrodes of a MEA. Both the MEA and the PDMS membrane were treated with O2 plasma,Poly-L-Lysine,and Laminin to aid in cell attachment and survival. The primary advantage of our process is that it is quicker and simpler than previous cell-placement methods and may make highly defined neuronal network biochips more practical.

关键词：表面处理技术激光器指导技术神经元生物芯片