CRISPR-powered optothermal nanotweezers:Diverse bio-nanoparticle manipulation and single nucleotide identification

作     者：Jiajie Chen Zhi Chen Changle Meng Jianxing Zhou Yuhang Peng Xiaoqi Dai Jingfeng Li Yili Zhong Xiaolin Chen Wu Yuan Ho-Pui Ho Bruce Zhi Gao Junle Qu Xueji Zhang Han Zhang Yonghong Shao Jiajie Chen;Zhi Chen;Changle Meng;Jianxing Zhou;Yuhang Peng;Xiaoqi Dai;Jingfeng Li;Yili Zhong;Xiaolin Chen;Wu Yuan;Ho-Pui Ho;Bruce Zhi Gao;Junie Qu;Xueji Zhang;Han Zhang;Yonghong Shao

作者机构：State Key Laboratory of Radio Frequency Heterogeneous IntegrationKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceCollege of Physics and Optoelectronics EngineeringShenzhen UniversityShenzhen 518060China Department of Biomedical EngineeringThe Chinese University of Hong KongShatinHong KongChina Department of Bioengineering and COMSETClemson UniversityClemsonSC 29634USA School of Biomedical EngineeringShenzhen UniversityShenzhenGuangdong 518060China 

出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)

年 卷 期：2023年第12卷第12期

页      面：2644-2654页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China(62275164,61905145,62275168,61775148) National Key Research and Development Program of China(No.2022YFA1206300) Guangdong Natural Science Foundation and Province Project(2021A1515011916,2023A1515012250) Foundation from Department of Science and Technology of Guangdong Province(2021QN02Y124) Shenzhen Science and Technology R&D and Innovation Foundation(JCYJ20200109105608771) Shenzhen Key Laboratory of Photonics and Biophotonics(ZDSYS20210623092006020) The Research Grants Council(RGC)of Hong Kong China(RGC14207920) Innovation Team Project of Department of Education of Guangdong Province(2018KCXTD026) Deanship of Scientific Research(DSR)at King Abdulaziz University,Jeddah(KEP-MSc-70-130-42) King Khalid University through Research Center for Advanced Materials Science(RCAMS)(RCAMS/KKU/006/21) Medical-Engineering Interdisciplinary Research Foundation of Shenzhen University 

主　　题：manipulation thermal identification 

摘      要：Optothermal nanotweezers have emerged as an innovative optical manipulation technique in the past decade,which revolutionized classical optical manipulation by efficiently capturing a broader range of ***,the optothermal temperature field was merely employed for in-situ manipulation of nanoparticles,its potential for identifying bio-nanoparticles remains largely ***,based on the synergistic effect of optothermal manipulation and CRIPSR-based bio-detection,we developed CRISPR-powered optothermal nanotweezers(CRONT).Specifically,by harnessing diffusiophoresis and thermo-osmotic flows near the substrate upon optothermal excitation,we successfully trapped and enriched DNA functionalized gold nanoparticles,CRISPR-associated proteins,as well as DNA ***,we built an optothermal scheme for enhancing CRISPR-based single-nucleotide polymorphism(SNP)detection at single molecule level,while also introducing a novel CRISPR methodology for observing nucleotide ***,this innovative approach has endowed optical tweezers with DNA identification ability in aqueous solution which was unattainable *** its high specificity and feasibility for in-situ bio-nanoparticle manipulation and identification,CRONT will become a universal tool in point-of-care diagnosis,biophotonics,and bio-nanotechnology.