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Enrichment of ER tubule-derived microsomes by differential centrifugation and immunoprecipitation

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Biophysics Reports 2024年第2期10卷 61-66页

作者： Yiduo Liu Junjie Hu bing yan National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences University of Chinese Academy of Sciences

The endoplasmic reticulum(ER) is an essential component of the endomembrane system in eukaryotes and plays a crucial role in protein and lipid synthesis, as well as the maintenance of calcium homeostasis. Morphologically, the ER is composed primarily of sheets and tubules. The tubular ER is composed of a network of tubular membrane structures, each with diameters ranging from 30 to 50nanometers. In recent years, there has been in-depth research on the molecular mechanisms of membrane shaping and membrane fusion of the tubular ER. However, there is still limited understanding of the specific physiological functions of the tubular ER. Here, we report a protocol that combines differential centrifugation and immunoprecipitation to specifically enrich microsomes originating from the tubular ER in yeast. The ER tubule-derived microsomes can be further used for proteomic and lipidomic studies or other biochemical analyses.

关键词： Endoplasmic reticulum Microsome Differential centrifugation Immunoprecipitation

Surface-induced Microstructure and Performance Changes in P3HT Ultrathin Films

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Chinese Journal of Polymer Science 2024年第6期42卷 805-814页

作者： Hong-Tao Shan Jia-Xin He bing-yan Zhu Xue-Ting Cao Ying-Ying yan Jian-Jun Zhou Hong Huo Beijing Key Laboratory of Energy Conversion and Storage Materials College of ChemistryBeijing Normal UniversityBeijing100875China

In this work,poly(3-hexylthiophene)(P3HT)ultrathin films(P3HT-T)were prepared by spin-coating a dilute P3HT solution(in a toluene:o-dichlorobenzene(Tol:ODCB)blend with a volume ratio of 80:20)with ultrasonication and the addition of the nucleating agent bicycle[2.2.1]heptane-2,3-dicarboxylic acid disodium salt(HPN-68L)on glass,Si wafers and indium tin oxide(ITO)*** electrical and mechanical properties of the P3HT-T ultrathin films were investigated,and it was found that the conductivity and crack onset strain(COS)were simultaneously improved in comparison with those of the corresponding pristine P3HT film(P3HT-0,without ultrasonication and nucleating agent)on the same substrate,regardless of what substrate was ***,the conductivity of P3HT-T ultrathin films on different substrates was similar(varying from 3.7 S·cm^(-1)to 4.4 S·cm^(-1)),yet the COS increased from 97%to 138%by varying the substrate from a Si wafer to *** grazing-incidence wide-angle X-ray diffraction(GIXRD),UV-visible(UV-Vis)spectroscopy and atomic force microscopy(AFM),we found that the solid order and crystallinity of the P3HT-T ultrathin film on the Si wafer are highest,followed by those on glass,and much lower on ***,the surface energy and roughness of three substrates were investigated,and it was found that the polar component of the surface energyγp plays a critical role in determining the crystalline microstructures of P3HT ultrathin films on different *** work indicates that the P3HT ultrathin film can obviously improve the stretchability and simultaneously retain similar electrical performance when a suitable substrate is *** findings offer a new direction for research on stretchable CP ultrathin films to facilitate future practical applications.

关键词： P3HT ultrathin film Substrate Crystalline microstructures Polar component of the surface energy Electrical and stretchable performances

Impact of climate and human activity on NDVI of various vegetation types in the Three-River Source Region, China

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Journal of Arid Land 2024年第8期16卷 1080-1097页

作者： LU Qing KANG Haili ZHANG Fuqing XIA Yuanping yan bing Research Center of Resource and Environment Economics East China University of TechnologyNanchang 330013China Jiangxi Key Laboratory of Watershed Ecological Process and Information East China University of TechnologyNanchang 330013China School of Surveying and Geoinformation Engineering East China University of TechnologyNanchang 330013China Nanchang Key Laboratory of Landscape Process and Territorial Spatial Ecological Restoration East China University of TechnologyNanchang 330013China Institute of Energy Research Jiangxi Academy of SciencesNanchang 330096China

The Three-River Source Region(TRSR)in China holds a vital position and exhibits an irreplaceable strategic importance in ecological preservation at the national *** the basis of an in-depth study of the vegetation evolution in the TRSR from 2000 to 2022,we conducted a detailed analysis of the feedback mechanism of vegetation growth to climate change and human activity for different vegetation *** the growing season,the spatiotemporal variations of normalized difference vegetation index(NDVI)for different vegetation types in the TRSR were analyzed using the Moderate Resolution Imaging Spectroradiometer(MODIS)-NDVI data and meteorological data from 2000 to *** addition,the response characteristics of vegetation to temperature,precipitation,and human activity were assessed using trend analysis,partial correlation analysis,and residual *** indicated that,after in-depth research,from 2000 to 2022,the TRSR's average NDVI during the growing season was *** preliminary ranking of the average NDVI for different vegetation types was as follows:shrubland(0.5762)>forest(0.5443)>meadow(0.4219)>highland vegetation(0.2223)>steppe(0.2159).The NDVI during the growing season exhibited a fluctuating growth trend,with an average growth rate of 0.0018/10a(P<0.01).Notably,forests displayed a significant development trend throughout the growing season,possessing the fastest rate of change in NDVI(0.0028/10a).Moreover,the upward trends in NDVI for forests and steppes exhibited extensive spatial distributions,with significant increases accounting for 95.23%and 93.80%,*** sensitivity to precipitation was significantly enhanced in other vegetation types other than highland *** contrast,steppes,meadows,and highland vegetation demonstrated relatively high vulnerability to temperature fluctuations.A further detailed analysis revealed that climate change had a significant positive impact on the TRSR from 2000 to 2022,particularly in its

关键词： growing season normalized difference vegetation index(NDVI) highland vegetation trend analysis partial correlation analysis residual analysis contribution rate

Action of circulating and infiltrating B cells in the immune microenvironment of colorectal cancer by single-cell sequencing analysis

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2683-2696页

作者： Jing-Po Zhang bing-Zheng yan Jie Liu Wei Wang Department of Hepatobiliary and Pancreatic Surgery First Hospital of Hebei Medical UniversityShijiazhuang 050032Hebei ProvinceChina Department of Hepatobiliary Surgery Hunan Provincial People’s HospitalChangsha 410002Hunan ProvinceChina

BACKGROUND The complexity of the immune microenvironment has an impact on the treatment of colorectal cancer(CRC),one of the most prevalent malignancies *** this study,multi-omics and single-cell sequencing techniques were used to investigate the mechanism of action of circulating and infiltrating B cells in *** revealing the heterogeneity and functional differences of B cells in cancer immunity,we aim to deepen our understanding of immune regulation and provide a scientific basis for the development of more effective cancer treatment *** To explore the role of circulating and infiltrating B cell subsets in the immune microenvironment of CRC,explore the potential driving mechanism of B cell development,analyze the interaction between B cells and other immune cells in the immune microenvironment and the functions of communication molecules,and search for possible regulatory pathways to promote the anti-tumor effects of B *** A total of 69 paracancer(normal),tumor and peripheral blood samples were collected from 23 patients with CRC from The Cancer Genome Atlas database(https://***/).After the immune cells were sorted by multicolor flow cytometry,the single cell transcriptome and B cell receptor group library were sequenced using the 10X Genomics platform,and the data were analyzed using bioinformatics tools such as *** differences in the number and function of B cell infiltration between tumor and normal tissue,the interaction between B cell subsets and T cells and myeloid cell subsets,and the transcription factor regulatory network of B cell subsets were explored and *** Compared with normal tissue,the infiltrating number of CD20+B cell subsets in tumor tissue increased *** them,germinal center B cells(GCB)played the most prominent role,with positive clone expansion and heavy chain mutation level increasing,and the trend of differentiation into memory B cells ***,the

关键词： Colorectal cancer Multi-omics analysis Single-cell sequencing analysis Immune microenvironment Infiltrating B cells

Alanine aminotransferase as a risk marker for new-onset metabolic dysfunction-associated fatty liver disease

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World Journal of Gastroenterology 2024年第25期30卷 3132-3139页

作者： Di Wang bing-yan Zhou Lei Xiang Xu-Yong Chen Jie-Xiong Feng Department of Pediatric Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030Hubei ProvinceChina

In this editorial,we comment on the article by Chen et *** dysfunction-associated fatty liver disease(MAFLD)is a global public health burden whose incidence has risen concurrently with overweight and *** its detri-mental health impact,early identification of at-risk individuals is *** diagnosis is based on evidence of hepatic steatosis indicated by liver biopsy,imaging,or blood biomarkers,and one of the following conditions:Overweight/obesity,type 2 diabetes mellitus,or metabolic ***,in large-scale epidemiological studies,liver biopsies are not *** application of techniques such as ultrasonography,computed tomography,magnetic resonance imaging,and magnetic resonance spectroscopy is restricted by their limited sensitivity,low effectiveness,high costs,and need for specialized *** biomarkers offer several advantages,particularly in large-scale epidemiological studies or clinical scenarios where traditional imaging techniques are *** of cumulative effects of excess high-normal blood alanine aminotrans-ferase(ALT)levels of blood ALT levels could facilitate identification of at-risk patients who might not be detected through conventional imaging ***,investigating the utility of blood biomarkers in MAFLD should enhance early detection and monitoring,enabling timely inter-vention and management and improving patient outcomes.

关键词： Nonalcoholic fatty liver disease Metabolic dysfunction-associated fatty liver disease Alanine aminotransferase Screening Risk marker

Organic Integration of Traditional Chinese and Western Medicines—Future of Clinical Practice Guidelines of Integrated Traditional Chinese and Western Medicines

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Chinese Journal of Integrative Medicine 2024年第4期30卷 359-365页

作者： XIE Ying-lan HAN Fei JIN Ying-hui DING yan-bing GUO Jing JI Dong-xiao ZHANG Teng CHENXiao-min SHANG Hong-cai Department of Traditional Chinese Medicine Encephalopathy Hubei Provincial Hospital of Traditional Chinese MedicineWuhan(430074)China Department of Traditional Chinese Medicine Encephalopathy Hubei Province Traditional Chinese Medicine Research InstituteWuhan(430074)China Xuanwu Hospital of Traditional Chinese Medicine Beijing(100000)China Center for Evidence-Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan(430074)China College of Acupuncture and Massage College of Health and RehabilitationNanjing University of Chinese MedicineNanjing(210023)China Department of Ophthalmology Huaihe Hospital of Henan UniversityKaifengHenan Province(475000)China Ministry of Education and Beijing Key Laboratory of Internal Medicine of Traditional Chinese Medicine Dongzhimen Hospital of Beijing University of Traditional Chinese Medicine/Ministry of Education and Beijing Key Laboratory of Internal Medicine of Traditional Chinese MedicineBeijing(100700)China

The transformation and implementation of clinical practice guidelines for integrated traditional Chinese medicine(TCM)and Western medicine(WM)is crucial to the adoption of medical science and technological findings and is an important way for TCM to be made available to the ***,clinical practice guidelines(CPGs)of TCM and WM integration in recent years was analyzed to clarify the current situation and problems in the existing guidelines according to the following four perspectives:(1)perspective of TCM and WM integration in guidelines,(2)diagnosis Using integrated TCM and WM,(3)integration of TCM and WM treatment,(4)promoting TCM and WM ***,the information and quality evaluation of CPGs for integrated Chinese and Western medicine in 2020–2022 were analyzed to explore the degree and methods of integration of Chinese and Western medicine *** last this study aimed to lay a foundation for the further establishment of Chinese characteristic,repeatable,and calculable clinical practice guidelines of TCM and WM integration.

关键词： clinical practice guidelines integrated traditional Chinese and Western medicine organic integration

Theoretical investigation of excited dipole bound states of alkali-containing diatomic anions

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Chinese Physics B 2024年第5期33卷 389-393页

作者：连艺肖利丹边丽丽徐海峰闫冰 Institute of Atomic and Molecular Physics Jilin UniversityChangchun 130012China

Information about electronic excited states of molecular anions plays an important role in investigating electron attachment and detachment *** we present a high-level theoretical study of the electronic structures of 12 alkali-metal-containing diatomic anions MX-(MX = LiH,LiF,LiCl,NaF,NaCl,NaBr,RbCl,KCl,KBr,RbI,KI and CsI).The equation-of-motion electron-attachment coupled-cluster singles and doubles(EOM-EA-CCSD) method is used to calculate the electron binding energies(EBEs) of 10 electronic excited states of each of the 12 molecule *** addition of different s-/p-/d-type diffusion functions in the basis set,we have identified possible excited dipole bound states(DBSs) of each *** the investigation of EBEs on the 12 MXs with dipole moment(DM) up to 12.1 D,we evaluate the dependence of the number of anionic excited DBSs on molecular *** results indicate that there are at least two or three DBSs of anions with a molecular DM larger than 7 D and a molecule with DM > 10 D can sustain a π-DBS of the *** study has some implications for the excited DBS electronic states of alkali-metal-containing diatomic molecules.

关键词： anions dipole bound states alkali-metal-containing diatomic molecules

贸易摩擦、不确定性感知与企业研发行为:来自中国上市公司的微观证据

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世界经济研究 2024年第3期 107-119,M0004页

作者：严兵齐凡南开大学跨国公司研究中心南开大学经济学院

文章基于2007~2020年上市公司年报数据,通过文本分析构建企业层面的贸易政策不确定性感知指数,从微观层面揭示了不同企业在感知到贸易政策不确定性后作出的异质化研发决策。论文发现,感知到贸易环境中不确定性的上升会促使企业提升研发... 详细信息

文章基于2007~2020年上市公司年报数据,通过文本分析构建企业层面的贸易政策不确定性感知指数,从微观层面揭示了不同企业在感知到贸易政策不确定性后作出的异质化研发决策。论文发现,感知到贸易环境中不确定性的上升会促使企业提升研发强度,这一效果主要体现在高科技行业。其中,企业去金融化与争取政府补贴是最主要的影响机制,为了获取税收优惠而人为调整会计科目造成的研发强度提升并不能形成真正的研发成果,因而不利于企业抵抗风险能力的提升。在此基础上,进一步研究发现,受美国对华关税加征的影响,中国高新技术上市企业研发强度显著提升,受影响企业正在努力开拓自主创新之路。

关键词：贸易摩擦贸易政策不确定性研发创新

e^(+)e^(-)→Λ_(c)+Λ_(c)^(- ) Cross Sections and the Λ_(c)^(+) Electromagnetic Form Factors within the Extended Vector Meson Dominance Model

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Chinese Physics Letters 2024年第2期41卷 36-41页

作者：陈诚闫冰谢聚军 Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China School of Nuclear Sciences and Technology University of Chinese Academy of SciencesBeijing 101408China College of Mathematics and Physics Chengdu University of TechnologyChengdu 610059China Southern Center for Nuclear-Science Theory(SCNT) Institute of Modern PhysicsChinese Academy of SciencesHuizhou 516000China

Within the extended vector meson dominance model,we investigate the𝑒e^(+)e^(-)→Λ_(c)+Λ_(c)^(- ) reaction and the electromagnetic form factors of the charmed baryon𝛬 Λ_(c)^(+)𝑐.The model parameters are determined by fitting them to the cross sections of the process𝑒e^(+)e^(-)→Λ_(c)+Λ_(c)^(- ) and the magnetic form factor|G_(M)|of Λ_(c)^(+) .By considering four charmonium-like states,called𝜓(4500),𝜓(4660),𝜓(4790),and𝜓(4900),we can well describe the current data on the𝑒e^(+)e^(-)→Λ_(c)+Λ_(c)^(- ) reaction from the reaction threshold up to 4.96 *** addition to the total cross sections and|𝐺M|,the ratio|G_(E)/G_(M)|and the effective form factor|Geff|for Λ_(c)^(+) 𝑐are also calculated,and found that these calculations are consistent with the experimental *** the fitted model parameters,we have also estimated the charge radius of the charmed Λ_(c)^(+) baryon.

关键词： fitted radius meson