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UBE2I stimulates female gonadal differentiation in chicken(Gallus gallus)embryos

Journal of Integrative Agriculture 2021年第11期20卷 2986-2994页

作者： JIN Kai ZHOU Jing ZUO Qi-sheng li Jian-cheng Jiuzhou SONG ZHANG Ya-ni CHANG Guo-bing CHEN Guo-hong li bi-chun Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province College of Animal Science and TechnologyYangzhou UniversityYangzhou 225009P.R.China Institutes of Agricultural Science and Technology Development Yangzhou UniversityYangzhou 225009P.R.China Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China Yangzhou UniversityYangzhou 225009P.R.China Animal&Avian Sciences University of MarylandCollege ParkMD20741USA

Without known analogous sex-determining factors like SRY(sex determining region Y)in mammals,the chicken(Gallus gallus)sex determination mechanism still remains unclear,which highly restricts the biological research on chicken development and poultry single-sex *** we not only characterized a new female-biased gene UBE2I and identified the expression pattern by qRT-PCR,but also described the functional role of UBE2I in the gonadal development of chicken *** showed that UBE2I exhibited a female-biased expression pattern in the early stage of PGCs(primordial germ cells)in embryonic gonads and robust expression in ovaries of newborn *** importantly,we successfully developed an effective method to interfere or overexpress UBE2I in chicken embryos through the intravascular *** qRT-PCR analysis showed that the sex-related genes(FOXL2,CYP19A1 and HINTW)in females were upregulated(P<0.05)under the overexpression of UBE2I and the sex-related genes(SOX9,DMRT1 and WT1)in females were downregulated(P<0.05)after interfering ***,the change of UBE2I expression was associated with the level of estradiol and its receptors(AR and ESR),which suggests that UBE2I is necessary to initiate the female-specific development in *** conclusion,this work demonstrates that UBE2I is a crucial sex differentiation-related gene in the embryonic development of chickens,which provides insights for further understanding the mechanism of sex determination in chickens.

关键词： UBE2I gonadal differentiation chicken embryonic sex differentiation

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Atmospheric Deposition-Carried Pb, Zn, and Cd from a Zinc Smelter and Their Effect on Soil Microorganisms

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Pedosphere 2009年第4期19卷 422-433页

作者： YANG Yuan-Gen2, JIN Zhi-Sheng, bi Xiang-Yang, li Fei-li, SUN li, liU Jie and FU Zhi-You Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002 (China) Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 (China)

Dust emissions from smelters, as a major contributor to heavy metal contamination in soils, could severely influence soil quality. Downwind surface soils within 1.5 km of a zinc smelter, which was active for 10 years but ceased in 2000, in Magu Town, Cuizhou Province, China were selected to examine Pb, Zn, and Cd concentrations and their fractionation along a distance gradient from a zinc smelter, and to study the possible effects of Pb, Zn, and Cd accumulation on soil microorganisms by comparing with a reference soil located at a downwind distance of 10 km from the zinc smelter. Soils within 1.5 km of the zinc smelter accumulated high levels of heavy metals Zn （508 mg kg^-1）, Pb （95.6 mg kg^-1）, and Cd （5.98 mg kg^-1） with low ratios of Zn/Cd （59.1-115） and Pb/Cd （12.4-23.4）. Composite pollution indices （CPIs） of surface soils （2.52-15.2） were 3 to 13 times higher than the reference soils. In metal accumulated soils, exchangeable plus carbonate-bound fractions accounted for more than 10% of the total Zn, Pb, and Cd. The saturation degree of metals （SDM） in soils within 1.5 km of the smelter （averaging 1.25） was six times higher than that of the reference soils （0.209）. A smaller soil microbial biomass was found more frequently in metal accumulated soils （85.1-438 μg C g^-1） than in reference soils （497 μg C g^-1）, and a negative correlation （P 〈 0.01） of soil microbial biomass carbon to organic carbon ratio （Cmic/Corg） with SDM was observed. Microbial consumption of carbon sources was more rapid in contaminated soils than in reference soils, and a shift in the substrate utilization pattern was apparent and was negatively correlated with SDM （R = -0.773, P 〈 0.01）. Consequently, dust deposited Pb, Zn, and Cd in soils from zinc smelting were readily mobilized, and were detrimental to soil quality mainly in respect of microbial biomass .

关键词： composite pollution index heavy metals microbial biomass saturation degree of metals sequential extrac- tion

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A Brief Analysis About the Function of the Communicative Approach -- It Can lighten Classroom Anxiety in Foreign Language Teaching

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Sino-US English Teaching 2006年第9期3卷 29-31页

作者： li bi Langfang Teachers College

Classroom Anxiety is very common and to some extent it influences students＇ achievements in foreign language learning. So, how to solve this problem in order to help students have a good performance in foreign language learning is a very urgent issue. According to the writer＇s study, communicative approach is an effective method to deal with it.

关键词： communicative approach classroom anxiety foreign language teaching

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Land Suitability Assessment and Land Use Change in Fujian Province,China

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Pedosphere 2007年第4期17卷 493-504页

作者： QUAN bin ZHU He-Jian CHEN Song-lin M. J. M. ROMKENS li bi-Cheng Research Center of Soil and Water Conservation and Ecological Environment Chinese Academy of Sciences and Mi- nistry of Education Northwest Sci-Tech University of Agriculture and Forestry Yangling Shaanxi 712100 （China）. Key Laboratory of Subtropical Resources and Environment of Fujian Province Fujian Normal University Fuzhou 350007 （China） USDA/ARS National Sedimentation Laboratory P. O. Box 1157 Oxford MS 38655 （USA）

To present the current status of land quality and distribution and the trends in land use change, the physical suitability of cropland and forestland and the associated changes in Fujian Province were evaluated and analyzed using data obtained from geographical information systems (GIS) and remote sensing (RS).Of the total land area of Fujian Province,first class suitable cropland accounted for only 4.21%, whereas unsuitable cropland accounted for 84.78%. The percentage of first class suitable cropland in the southeastern region (5.32%) was much higher than that in the northwestern area (2.91%).Only 13.63% of the existing cropland area consisted of first class cropland and 70.08% was classified as unsuitable for *** the total land area of Fujian Province,the first class forestland comprised 55.25% and the unsuitable forestland (including third class) comprised 21.2%.The percentage of unsuitable forest area in the existing forestland was only 5.5%.From 1996 to 2001, cropland and unused land decreased significantly, whereas forestland and land used for urban and transport increased ***,the major tasks ahead will be the land development for full grain production potential,the better coordination of allocating land to different uses, and the regulation inappropriate activities that damage agricultural ecosystems.

关键词： Fujian Province GIS land evaluation land use change remote sensing

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An efficient and rapid method to detect and verify natural antisense transcripts of animal genes

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Journal of Integrative Agriculture 2016年第9期15卷 2070-2076页

作者： ZHANG li ZHAO Rui XIAO Mei liN Shu-dai li bi-xiao QIU Feng-fang MA Jing-e ZHANG De-xiang NIE Qing-hua AN li-long ZHANG Xi-quan Agricultural College Guangdong Ocean University Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding/Key Lab of Chicken Genetics Breeding and Reproduction Ministry of Agriculture/South China Agricultural University

High-throughput sequencing has identified a large number of sense-antisense transcriptional pairs, which indicates that these genes were transcribed from both directions. Recent reports have demonstrated that many antisense RNAs, especially lnc RNA（long non-coding RNA）, can interact with the sense RNA by forming an RNA duplex. Many methods, such as RNA-sequencing, Northern blotting, RNase protection assays and strand-specific PCR, can be used to detect the antisense transcript and gene transcriptional orientation. However, the applications of these methods have been constrained, to some extent, because of the high cost, difficult operation or inaccuracy, especially regarding the analysis of substantial amounts of data. Thus, we developed an easy method to detect and validate these complicated RNAs. We primarily took advantage of the strand specificity of RT-PCR and the single-strand specificity of S1 endonuclease to analyze sense and antisense transcripts. Four known genes, including mouse β-actin and Tsix（Xist antisense RNA）, chicken LXN（latexin） and GFM1（Gelongation factor, mitochondrial 1）, were used to establish the method. These four genes were well studied and transcribed from positive strand, negative strand or both strands of DNA, respectively, which represented all possible cases. The results indicated that the method can easily distinguish sense, antisense and sense-antisense transcriptional pairs. In addition, it can be used to verify the results of high-throughput sequencing, as well as to analyze the regulatory mechanisms between RNAs. This method can improve the accuracy of detection and can be mainly used in analyzing single gene and was low cost.

关键词： natural antisense transcripts transcription orientation detection method RNA sequencing long non-coding RNA

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Marker-assisted selection of YY supermales from a genetically improved farmed tilapia-derived strain

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Zoological Research 2019年第2期40卷 108-112页

作者： Chao-Hao Chen bi-Jun li Xiao-Hui Gu Hao-Ran lin Jun-Hong Xia State Key Laboratory of biocontrol Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals College of Life Sciences Sun Yat-Sen University

DEAR EDITOR, Genetically improved farmed tilapia (GIFT)and GIFT-derived strains account for the majority of farmed tilapia *** male tilapias grow much faster than females,they are often considered more desirable in the aquacultural *** reversal of females to males using the male sex hormone 17-α-methyltestosterone (MT)is generally used to induce phenotypic males during large-scale production of all male ***,the widespread use of large quantities of sex reversal hormone in hatcheries may pose a health risk to workers and ecological threats to surrounding environments. Breeding procedures to produce genetically all-male tilapia with limited or no use of sex hormones are therefore urgently needed.

关键词： tilapia-derived strain genetically improved

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Transthoracic arterial bypass for the treatment of severe cerebral ischemia induced by brachiocephalic arteritis: A report of 15 cases

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Neural Regeneration Research 2007年第11期2卷 692-697页

作者： Chenxi Ouyang bi Jin Yiqing li Weici Wang Guofu Hu Deying Hu Fen Xie Department of Vascular Surgery Union Hospital Affiliated to Tongji Medical College Huazhong University of Science and Technology Wuhan430022 Hubei Province China

BACKGROUND： Carotid arterial reconstruction is hard to cure the extensive lesions of carotid arteries and subclavian arteries in patients with brachiocephalic arteritis; however, transthoracic arterial bypass surgery provides an opportunity for the treatment of brachiocephalic arteritis. OBJECTIVE： To report the improving effects of transthoracic arterial bypass surgery on the clinical symptoms of severe cerebral ischemia induced by brachiocephalic arteritis and observe the occurrence of complications after the intervention. DESIGN： Case observation. SETTING： Department of Vascular Surgery, Union Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology. PARTICIPANTS： Fifteen patients with Takayasu arteritis, including 1 male and 14 females, were selected from Department of Vascular Surgery, Wuhan Union Hospital from June 2003 to June 2007. Their ages ranged from 16 to 37 years. All patients were finally diagnosed by clinical histories, health examinations and color Doppler detection. All patients provided the confirmed consent. METHODS： All patients had received transthoracic artificial vascular bypass surgery. Among them, 6 patients had the bridge from ascending aorta to bilateral axillary arteries to lateral internal carotid artery; 3 from ascending aorta to bilateral axillary arteries; 5 from ascending aorta to lateral axillary artery to lateral internal carotid artery; In particular, one patient combining with abdominal aorta occlusion had received two step surgical interventions. In the first step, bridging surgery was performed from ascending aorta to bilateral axillary arteries to lateral internal carotid artery; in the second step, patients received left axillofemoral bypass. MAIN OUTCOME MEASURES：① Detecting blood velocity in variously intracranial-arterial systoles by using transcranial Doppler postoperatively; ② following-up the improve of clinical symptoms at 40 months after surgery; ③ observing postoperative c

关键词： Takayasu arteritis cerebral ischemia vascular bypass surgery

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FLEXIBLE NEW FCC TECHNOLOGY TO MEET THE EVER CHANGING MARKET DEMAND

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石油学报(石油加工) 2000年第5期16卷 82-84页

作者： CHEN Zu bi,li Cai ying (Research Institute of Petroleum Processing,Beijing 100083,China) Research Institute of Petroleum Processing SIN OPEC 北京 100083

Being a main conversion process,FCC is evolving toward processing more heavier and dirtier feeds,and to produce varied product slates to meet the local market *** many of the product demands made on the FCC process operator are seemingly ***,with the advance of FCC process and catalyst manufacture technology over the past few years,these contradictories can be solved *** VR FCC,maximized distillate production(MDP) technique and the maximizing gas and distillate(MGD) process are the *** data of each example will be revealed and shown on the poster.

关键词： FCC technology market demand

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External calibrator in global signal experiment for detection of the epoch of reionization

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Research in Astronomy and Astrophysics 2021年第10期21卷 17-26页

作者： Yan Huang Xiang-Ping Wu Quan Guo Qian Zheng bi-Ying li Huan-Yuan Shan Ke-Jia Lee Hai-Guang Xu National Astronomical Observatories Chinese Academy of SciencesBeijing 100101China Shanghai Astronomical Observatory Chinese Academy of SciencesShanghai 200030China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China School of Physics and Astronomy Shanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China

We present a conceptual design study of external calibrators in the 21 cm experiment towards detecting the globally averaged radiation of the epoch of reionization(EoR).Employment of external calibrator instead of internal calibrator commonly used in current EoR experiments allows removing instrumental effects such as beam pattern,receiver gain and instability of the system if the conventional three-position switch measurements are implemented in a short time ***,in the new design the antenna system is placed in an underground anechoic chamber with an open/closing ceiling to maximally reduce the environmental effect such as RFI and ground radiation/*** appears that three of the four external calibrators proposed in this paper,including two indoor artificial transmitters and one outdoor celestial radiation(the Galactic polarization),fail to meet our *** motion of the Galactic diffuse emission turns out to be the most probable source as an external calibrator,for which we have discussed the observational strategy and the algorithm of extracting the EoR signal.

关键词： cosmology:observations dark ages reionization radio lines:general methods:observational

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Metallogenesis and ore-forming time of the Changtuxili Mn-Ag-Pb-Zn deposit in Inner Mongolia:Evidence from C-O-S isotopes and U-Pb geochronology

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Geoscience Frontiers 2020年第4期11卷 1369-1380页

作者： Kuo Zhang Ruo-Shi Jin Feng-Yue Sun bi-Le li Peng He Yue-Long Zhang Shuo Guo Tian-Fu Zhang College of Earth Sciences Jilin UniversityChangchun130061China Tianjin Center China Geological SurveyTianjin300170China North China Center for Geoscience Innovation Tianjin300170China Qiqihar Branch Heilongjiang Institute of Geological Survey and ResearchQiqihar150036China

This paper reports new geochronological(U-Pb) and isotope(C,O,and S) data to investigate the timing of mineralization and mode of ore genesis for the recently discovered Changtuxili Mn-Ag-Pb-Zn deposit,located on the western slopes of the southern Great Hinggan Range in NE *** mineralization is hosted by intermediate-acidic lavas and pyroclastic rocks of the Baiyingaolao *** stages of mineralization are identified:quartz-pyrite(Stage I),galena-sphalerite-tetrahedrite-rhodochrosite(Stage II),and quartz-pyrite(Stage Ⅲ).δ13C and δ18O values for carbonate from the ore vary from-8.51‰ to-4.96‰ and 3.97‰ to 15.90‰,respectively,which are indicative of a low-temperature alteration environment.δ34SV-CDT values of sulfides range from-1.77‰ to 4.16‰ and show a trend of equilibrium fractionation(δ34SPy>δ34SSp>δ34SGn).These features indicate that pyrite,sphalerite,and galena precipitated during the period of *** alteration mineral assemblage and isotope data indicate that the weakly acidic to weakly alkaline ore-forming fluid was derived largely from meteoric water and the ore-forming elements C and S originated from *** the mineralization,a geochemical barrier was formed by changes in the pH of the ore-forming fluid,leading to the precipitation of *** the basis of the mineralization characteristics,new isotope data,and comparison with adjacent deposits,we propose that the Changtuxili Mn-Ag-Pb-Zn deposit is an intermediate-to lowsulfidation epithermal deposit whose formation was controlled by fractures and variability in the pH of the oreforming *** surrounding volcanic rocks yield zircon U-Pb ages of 160-146 Ma(Late Jurassic),indicating that the mineralization is younger than 146 Ma.

关键词： Inner Mongolia Changtuxili Mn-Ag-Pb-Zn deposit C-O-S isotope geochemistry Zircon U-Pb age Intermediate-to low-sulfidation epithermal deposit

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