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食品科技 2011年第1期36卷 246-249页

作者：崔亚娟姜菲菲唐华澄李东北京市营养源研究所分析检测中心北京100069

建立了食品中VD含量测定的方法,样品经皂化、石油醚萃取后,正相半制备HPLC净化,反相HPLC定量分析,内标法定量。方法的平均回收率为94.4%,RSD为4.58%;精密度RSD为0.56%;两个样品重现性RSD为1.95%和2.52%。采用该方法分析了奶粉、AD钙奶... 详细信息

建立了食品中VD含量测定的方法,样品经皂化、石油醚萃取后,正相半制备HPLC净化,反相HPLC定量分析,内标法定量。方法的平均回收率为94.4%,RSD为4.58%;精密度RSD为0.56%;两个样品重现性RSD为1.95%和2.52%。采用该方法分析了奶粉、AD钙奶、豆奶粉、蛋白粉、强化饼干等5种食品中VD含量,该法可用于食品中VD含量的测定。

关键词：半制备-高效液相色谱法内标法 VD 食品

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Roles of TRPC3/6/7 channels in a rat model of inflammatory pain

Roles of TRPC3/6/7 channels in a rat model of inflammatory p...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Jing DING~1,Xiu-Yu CUI~1,Jun CHEN~(1,2,*) 1 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China 2 Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders,Tangdu Hospital,Fourth Military Medical University,Xi’an 710038,China

Background:The transient receptor potential canonical(TRPC) channels,which are abundantly expressed in central nervous system(CNS),are Ca permeable nonselective cation channels with different biological *** studies indicated that TRPC1 and TRPC6 channels were likely to be involved in the pathological process of mechanical hyperalgesia and nociceptor *** whether TRPC channels are involved in melittin- or formalin-induced inflammatory nociceptive responses remains ***:In this study,we used behavioral tests combined with pharmacological manipulations to explore the potential roles of TRPC channels in inflammatory pain and ***:In this study,we used the well-established bee-venom(melittin) and formalin *** flinch test was to examine whether TRPC channels are involved in inflammatory paininduced primary *** pain sensitivity of rats was determined by testing paw withdrawal thermal latency(PWTL,s) in response to heat stimuli applied to the injected *** pain hypersensitivity was tested as the paw withdrawal mechanical threshold(PWMT,g).Results:Post- and pre-treatment of the primary injury site with *** of SKF96365,a TRPC3/6/7 antagonist,significantly suppressed the development of inflammatory pain-evoked persistent spontaneous nociception(PSN) and hypersensitivity(thermal and mechanical). Whereas local administration of SKF96365 into the contralateral hindpaw produced no significant effect on any of the pain-associated *** together,these data indicate that TRPC3/6/7 channels might be involved in pathophysiological processing associated with inflammatory ***:In conclusion,we demonstrated for the first time that,although SKF96365 do not affect baseline thermal latency and mechanical nociceptive threshold,it reversed the inflammatory pain-induced spontaneous nociception,heat and mechanical hyperalgesia, indicating that TRPC3/6/7 channels might pla

关键词： TRPC channels melittin formalin test persistent spontaneous nociception primary thermal hyperalgesia primary mechanical hyperalgesia

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Potential regulatory role of calsequestrin in platelet Ca2+ homeostasis and its association with platelet hyperactivity in diabetes mellitus

Potential regulatory role of calsequestrin in platelet Ca2+ ...

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第八届海峡两岸心血管科学研讨会

作者： Zhi-xiang Zhu~1,Xin-feng Yu~1,Li-hong Chen~1, He-ping Zhang~2,Yong-quan Wu~2,Da-li Luo~1 (1.Department of Pharmacology,Capital Medical University,beijing 100069,China 2.Department of Cardiology,beijing Friendship Hospital Affiliated to Capital Medical University,beijing 100050,China)

Background Disordered intracellular Ca([Ca]) homeostasis may contribute significantly to platelet hyperactivity in aggregation and adhesion,thereby to cardiovascular complications in diabetes patients. Calsequestrin(CSQ),a Ca binding protein in sarco/endoplasmic reticulum(SER),can function as a regulator for Ca release in *** Calsequestrin(CSQ),a Ca binding protein in sarcoplasmic reticulum, can act as a regulator for Ca release in *** hypothesized that CSQ also functions in platelet, but somehow alters and contributes to Ca abnormality due to *** CSQ expression in platelets from streptozotocin - induced type 1 diabetes rats,and type 2 diabetes volunteers and Goto - Kakizaki rats were analyzed by Western blot and RT - ***[Ca] and aggregation were evaluated with Fura -2 and by aggregometer,*** Abundant CSQ protein was found in platelets from both rat and *** to controls,diabetic platelets from both patients and rats exhibited significantly lower CSQ expression at protein and mRNA levels,whereas other SER Ca regulatory proteins remained unchanged. As reported,hyperactive Ca and aggregation responses to agonists were observed in diabetic platelets. Additionally,an increased basal[Ca] at rest,which was largely dependent on intracellular Ca source,was also found in diabetic *** CSQ expression by miRNA elicited an enhanced basal [Ca] and an exaggerated Ca release response to agonist in HEK293 cells,while trifluoperazine to impair CSQ function caused a concentration - dependent[Ca] increase in normal *** Down-regulation of CSQ found in diabetic platelets and impairment of CSQ in normal platelets or HEK293 cells all showed disturbed Ca release at rest and upon stimulation,demonstrating a potential role of CSQ in regulation of platelet[Ca] homeostasis and involvement in diabetic platelet hyperactivity.

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Spatiotemporal cortical plasticity in response to persistent nociception

Spatiotemporal cortical plasticity in response to persistent...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： JunCHEN~(1,2,*) 1 Institute for Biomedical Sciences of Pain,Fourth Military Medical University,Xi''an 710038,China 2 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China

＜正＞Pain is composed of multiple dimensions including sensory-discriminative,affective-motivational and cognitive -evaluative *** it has been gradually known that nociceptive information is processed through widelydistributed, hierachically-interconnected neural network,referred to as neuromatrix,in the ***,the knowledge of pain perception,emotion and cognition at cellular and network level is surprisingly poor and requires to be gained by experimental research using multiple approaches at the cerebral *** the other hand,nociception,when persists,may result in comorbidities of pain such as anxiety,loss of sleep,amnesia,depression,*** still lack of effective therapeutic approaches in *** studies on the effects of persistent or chronic pain on cerebral cortical functions and structures are still *** this symposium,I would like to present some update data showing how persistent nociception influences cerebral cortices including somatosensory cortex area 1（SS1）,anterior cingulate cortex（ACC） and hippocampal formation（HF） which are believed to be involved in various functions of the *** using in vivo evoked potential recordings,in vitro multi-electrode-array（8x8 MEA） and whole-cell patch-clamp recordings on acutely prepared brain slices of rats suffering from persistent nociception,synaptic responses were significantly enhanced not only temporally （LTP-like） but also spatially（network enlargement） in SS 1 and HF,however in ACC,spatial enlargement of synaptic connections were observed,but LTP-like phenomenon was not significantly ***,the altered synaptic connections and functions at the cerebral levels were demonstrated to be caused by peripheral persistent nociception because blockade of impulses in the periphery could eliminate the cerebral changes *** single synaptic level, it was further shown that imbalance between excitatory（EAA） and inhibitory（IAA） amino acids at pre-synaptic components an

关键词： persistent nociception pain perception cortical plasticity

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Transport of neuroprotective flavonoids using blood-brain barrier cell,Caco-2 cell model and everted rat intestinal sacs based on cell toxicity

Transport of neuroprotective flavonoids using blood-brain ba...

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中国药理学会第十一次全国学术会议

作者： YANG Yu-ya,BAI Lu,LI Xiao-rong,LI Yu-hang,WANG Li-juan,XUE Ming (Department of Pharmacology,School of Basic Medical Sciences,Capital Medical University, beijing 100069,China)

OBJECTIVE Comparative evaluations of cellular toxicity,lipophilicity and in vitro transmembrane transport of eight cerebrovascular protection flavonoids including puerarin,rutin,hesperidin,quercetin,genistein,kaempferol,apigenin and isoliquiriti-genin were investigated using rat blood-brain barrier(BBB) cell,Caco-2 cell monolayer and everted intestine jejunum sac model. METHODS The BBB bilayer was successfully co-cultured via rat brain microvascular endothelial cells and *** apparent permeability coefficients(P) of the flavonoids were calculated from unilateral transport assays in Transwell system with simultaneous determination using a high performance liquid *** toxicity was determined by the MTT *** The tested flavonoids exhibited time-dependent P values in these models,suggesting the transmembrane transport of these compounds by passive diffusion *** were some differences in the P of flavonoids between the BBB cell model and the Caco-2 cell *** transport of the tested flavonoid could be enhanced when administrated with the flavonoid *** efflux mechanisms related with P-glycoprotein may exist with some *** permeability was correlated to the parental skeleton,the number and position of glycoside and hydroxyl groups of the *** These findings provided important information for establishing the transport relationship and predicting the oral bioavailability of the active flavonoids.

关键词： flavonoids blood-brain barrier cell Caco-2 cell monolayer everted rat intestinal sacs transport toxicity

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名人代言虚假食品广告法律责任初探

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中国卫生法制 2009年第6期17卷 11-14页

作者：梁海伦首都医科大学卫生法学系北京100069

《食品安全法》正式开始实施之后,如何界定名人代言行为、明晰代言人的法律地位及其责任,是目前需要深入探讨的,本文拟对名人代言虚假食品广告应承担法律责任作初步探析。首先从三个关键问题入手:第一是明确名人在食品广告中的法律地位... 详细信息

《食品安全法》正式开始实施之后,如何界定名人代言行为、明晰代言人的法律地位及其责任,是目前需要深入探讨的,本文拟对名人代言虚假食品广告应承担法律责任作初步探析。首先从三个关键问题入手:第一是明确名人在食品广告中的法律地位,这是明晰名人应担义务,划分名人承担责任的前提。第二是名人代言虚假食品广告是否承担侵权责任,本文从构成侵权行为的违法行为、损害后果、因果关系和主观过错的四个要件进行分析。第三是确认名人代言虚假食品广告承担共同责任的形态。在解决以上三个关键问题的基础上,笔者认为将所有名人代言虚假食品广告的行为均归为共同侵权行为,承担连带责任的论断值得商榷。对此问题需要从名人代言虚假广告行为中获得的利益、主观恶性程度及名人认知的局限性等方面进行分析。笔者提出了名人代言虚假广告应承担侵权补充责任的建议。

关键词：名人虚假广告侵权责任共同责任连带责任补充责任

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The preparation of polycolonal antiserum against pink1 and expression of it in rat brain

The preparation of polycolonal antiserum against pink1 and e...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Ji-Sheng LI~1,Yao-Hua LI~2,Xin LI~2,Shun YU~2,Nian-Qiang WANG~1,Xiao-Hong SUO~1,Hui YANG~(1,*) 1 beijing Institute for Neuroscience,Capital Medical University,beijing Center for Neural Regeneration & Repair,Key Laboratory for Neurodegenerative Disease of the Ministry of Education,beijing 100069,China 2 Department of Neurobiology,beijing Institute of Geriatrics,Key Laboratory for Neurodegenerative Diseases of Ministry of Education,Xuanwu Hospital of Capital University of Medical Sciences,beijing 100053,China

＜正＞Objective To investigate expression of PD related gene PINK1 in rat *** To obtain the antiserum against human PINK1,two New Zealand White rabbits（2.5 kg）were immunized with recombinant His-tag fusion human PINK1 protein;Purified by salt fractionation;The specificity and sensitivity of the antiserum were verified by Western blotting;The location and distribution were further determined by *** The human recombinant PINK1 gene was highly expressed as inclusions in prokaryotic expression system;After first salt fractionation, western blot analysis demonstrated that the antiserum detected a 40 kDa band in recombinant human PINK1 protein and rat brain homogenates which is anticipated;Immunohistochemical staining on normal adult rat brain sections showed that the PINK1-immunoreactive substance presents in rat brain bulbus olfactorius,cortex,hippocampus,striatum,cerebellum, substantia nigra and red *** Prepared a polycolonal antiserum against human PINK1 and the PINK1- immunoreactive substance was widely distributed in rat *** antiserum provided an useful experimental tool to profoundly research the tissue expression profile,intercellular location and biological function of PINK1.

关键词： PINK1 polycolonal antiserum rat recombinant protein

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The effect of age and gender on SD rats’ learning and memory abilities in Morris Water Maze

The effect of age and gender on SD rats’ learning and memor...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Ke-Rui GONG,She-Wei GUO,Ming-Gang LIU,Xiao-Yan ZHAO,Lai-Hong YAN,Li-Juan TIAN,Ying CHANG,Jun CHEN Institutes for Biomedical Sciences of Pain,Chinese Capital Medical University,beijing 100069

＜正＞Aim The effects of age and gender on Sprague-Dawley（SD） rats’ learning and memory were studied with Morris Water *** By using Morris Water Maze monitoring and analyzing system,we evaluated the learning and memory abilities of neonatal and adult SD rats *** to the development rules of SD rats,we chose the rats 28 d old as neonatal rats and 2 months old as adult *** rats were divided into four groups by age and gender: 1) female SD rats of 28 d old,2) male SD rats of 28 d old,3) female SD rats of 2 months old and 4) male SD rats of 2 months *** experiment included the hidden platform test which lasted for the first four days and the probe test on the fifth day. Escape distance of the hidden platform test,time percentage spent in target quadrant and platform-crossing times of the probe test were chose to evaluate the performance of the rats in Morris Water *** 1) For the SD rats 28 d old,after the four days’ training,the escape distance in both groups decreased greatly,but there was no significant difference between the two groups（male ***）.In the probe test,the time percentage spent in the target quadrant for both groups was higher than the other quadrants,but there was still no significant difference between female and *** the platform-crossing times,there was also no difference between the two groups.2) For the SD rats 2 months old,there was no significant difference between male and female in the escape distance,time percentage spent in the target quadrant and platform-crossing times as well.3) For the rats in the same gender,male or female,there was no statistic difference between the two age groups（28 d vs.2 m） in the escape distance,time percentage spent in the target quadrant and platformcrossing ***:As for the SD rats of 28 d and 2 months old,the age and gender probably have no significant effects on the learning and memory abilities in Morris Water Maze.

关键词： Sprague-Dawley rat Morris Water Maze learning and memory gender age

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Perinuclear calcium waves induced by activation of α1-adrenoceptors underlie autonomous nuclear calcium regulation in developing cardiomyocytes

Perinuclear calcium waves induced by activation of α1-adren...

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The 15th World Congress of Pharmacology

作者： Dali Luo Xiaomei Lan Kaitao Li Youyi Zhang Rui-Ping Xiao Qide Han Heping Cheng Institute of Cardiovascular Science at Health Science Center Peking University beijing 100083 China. Institute of Molecular Medicine and College of Life Science Peking University beijing 100871 China. Department of Pharmacology School of Chemical Biology & Pharmaceutical Sciences Capital University of Medical Sciences beijing 100069 China.

＜正＞Dynamic nuclear Ca2+ signals play pivotal roles in diverse cellular functions including gene transcription, cell growth, differentiation, and apoptosis. Here we report a novel nuclear Ca2+ regulatory mechanism that is mediated by perinuclear inositol 1,4,5-trisphosphate receptors （IP3Rs） in developing cardiac myocytes. Activation of IP3Rs by α1-adrenergic receptor （α1-AR） stimulation or by IP, application （in saponin-permeabilized cells） increases Ca2+ spark frequency preferentially in the perinuclear region in neonatal rat ventricular myocytes. Strikingly, we observed "perinuclear Ca2+ waves" that engulf the entire nucleus without spreading into the bulk cytosol. A perinuclear enrichment of IP3Rs supports the hyperexcitability of Ca2+-induced Ca2+ release in the layer of perinuclear sarcoplasmic reticulum （SR）. α1-AR stimulation enhances the formation of IP, and occurrence of perinuclear Ca2+ waves and confers them the ability to trigger cytosolic Ca2+ waves via IP3R-dependent pathways. This finding accounts for a profound frequency-dependent modulation of global Ca2+ oscillation during α1AR stimulation. Thus, IP3R Ca2+ waves traveling in the layer of perinuclear SR provide active, autonomous regulation of nuclear Ca2+ signaling, and serve as a pacemaker to drive global Ca2+ oscillation in the context of α1AR stimulation in developing cardiac myocytes.

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南刘寄奴中的一个新1,10-裂环愈创木烷型倍半萜(英文)

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中国天然药物 2012年第5期10卷 358-362页

作者：昝珂陈筱清屠鹏飞北京大学药学院天然药物及仿生药物国家重点实验室北京100191 首都医科大学中医药学院北京100069

目的:研究南刘寄奴中化学成分。方法:采用硅胶和 ODS 等柱层析分离手段以及半制备高效液相, 运用 NMR、MS 以及理化性质鉴定化合物的结构。结果:从南刘寄奴中分离得到 8 个化合物, 其结构鉴定为 3β-ethoxytanapartholide (1), ((4S*,5S... 详细信息

目的:研究南刘寄奴中化学成分。方法:采用硅胶和 ODS 等柱层析分离手段以及半制备高效液相, 运用 NMR、MS 以及理化性质鉴定化合物的结构。结果:从南刘寄奴中分离得到 8 个化合物, 其结构鉴定为 3β-ethoxytanapartholide (1), ((4S*,5S*)-dihydro-5-[(1R*, 2S*)-2-hydroxy-2-methyl-5-oxo-3-cyclopenten-1-yl]-3-methylene-4-(3-oxobutyl)-2(3H)-furanone) (2), ligucy-peronol (3), cyperusol C (4), santamarin (5), 1α, 2α, 3α, 4α, 10α-pentahydroxyguaia-11(13)-ene-12, 6α-olide (6), balanophonin (7),methyl 3-(2’-hydroxy-4’-methoxyphenyl) propanoate (8)。结论:化合物 1 是新人工产物, 化合物 4 和 8 为首次从蒿属中分离得到, 化合物 2–3, 5–7 为首次从该植物中分离得到。

关键词：南刘寄奴蒿属化学成分 1,10-裂环愈创木烷型倍半萜

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