Roles of TRPC3/6/7 channels in a rat model of inflammatory pain
会议名称：《中国生理学会第23届全国会员代表大会暨生理学学术大会》
会议日期：2010年
学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
关 键 词：TRPC channels melittin formalin test persistent spontaneous nociception primary thermal hyperalgesia primary mechanical hyperalgesia
摘 要：Background:The transient receptor potential canonical(TRPC) channels,which are abundantly expressed in central nervous system(CNS),are Ca permeable nonselective cation channels with different biological *** studies indicated that TRPC1 and TRPC6 channels were likely to be involved in the pathological process of mechanical hyperalgesia and nociceptor *** whether TRPC channels are involved in melittin- or formalin-induced inflammatory nociceptive responses remains ***:In this study,we used behavioral tests combined with pharmacological manipulations to explore the potential roles of TRPC channels in inflammatory pain and ***:In this study,we used the well-established bee-venom(melittin) and formalin *** flinch test was to examine whether TRPC channels are involved in inflammatory paininduced primary *** pain sensitivity of rats was determined by testing paw withdrawal thermal latency(PWTL,s) in response to heat stimuli applied to the injected *** pain hypersensitivity was tested as the paw withdrawal mechanical threshold(PWMT,g).Results:Post- and pre-treatment of the primary injury site with *** of SKF96365,a TRPC3/6/7 antagonist,significantly suppressed the development of inflammatory pain-evoked persistent spontaneous nociception(PSN) and hypersensitivity(thermal and mechanical). Whereas local administration of SKF96365 into the contralateral hindpaw produced no significant effect on any of the pain-associated *** together,these data indicate that TRPC3/6/7 channels might be involved in pathophysiological processing associated with inflammatory ***:In conclusion,we demonstrated for the first time that,although SKF96365 do not affect baseline thermal latency and mechanical nociceptive threshold,it reversed the inflammatory pain-induced spontaneous nociception,heat and mechanical hyperalgesia, indicating that TRPC3/6/7 channels might pla