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Quantitative evaluation of dose-effect of subcutaneous injection of bee venom on persistent nociception,pain hypersensitivity and inflammatory responses in rats

Quantitative evaluation of dose-effect of subcutaneous injec...

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Jia-Rui ZHANG~1,Yun-Fei LV~1,Yan WANG~1,Jun CHEN~(1,2,*) 1 Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders,Tangdu Hospital,The Fourth Military Medical University,Xi''an 710038,China 2 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China

＜正＞Objective The bee venom test has long been used to evaluate the antinociceptive,antihyperalgesic and anti-inflammatory effects of endogenous and exogenous substances,however,the dose-effect of subcutaneous（s.c.） injection of bee venom on each pain-related behavior has not been ***,in the current report we used six doses（0.001,0.005,0.01,0.05,0.1,and 0.2 mg in 50 uL）of bee venom solution to see whether there are differences in *** Quantitative methods were used for evaluation of each pain-related *** the number of paw flinches of each 5-min time block from 0-60 min was used to evaluate the intensity of persistent spontaneous nociception,while paw withdrawal thermal latency（PWTL,s）and paw withdrawal mechanical threshold （PWMT,g）were used to evaluate thermal and mechanical hyperalgesia,*** of paw volume, cross-dimensional paw thickness,skin temperature and volume of plasma extravasation were used to evaluate local inflammatory *** Generally,there were significant differences in peak responses between persistent spontaneous nociception,PWTL,PWMT,and local inflammation following *** of bee ***, there was a dose-dependent effect of bee venom on the number and time period of paw flinches,and the dose-effect curve showed a typical ’S’ that reached plateau at 0.1 ***,the peak%maximal possible effect（MPE）of bee venom on the reduction in PWTL and PWMT at 2 h after injection was caused by different doses of bee venom, namely 0.05 mg for PWTL and 0.01 mg for *** results suggest that the dose required for%MPE is different from each other in terms of persistent spontaneous nociception,thermal hyperalgesia and mechanical hyperalgesia. Furthermore,the dose for%MPE of four quantitative measurements was also different between paw volume（or thickness）,plasma extravasation and skin *** dose of 0.1 mg was required for%MPE of both paw volume and paw thickness,wh

关键词： bee venom test dose-effect persistent spontaneous nociception thermal hyperalgesia mechanical hyperalgesia local inflammation

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Properties of C-fiber mediated network synaptic responses in the spinal cord dorsal horn of rats:a multi-electrode array recording

Properties of C-fiber mediated network synaptic responses in...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Zhen LI~1,Zhen-Yu ZHAO~1,Xue-Feng CHEN~1,Rui-Rui WANG~1,Jun CHEN~(1,2*) 1 Institute for Biomedical Sciences of Pain,Tangdu Hospital,Fourth Military Medical University,Xi''an 710038,China 2 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China

＜正＞The spinal cord dorsal horn is the key site for transmission and modulation of nociceptive information via synaptic connections between primary nociceptive afferent C-fibers and secondary order sensory ***, little is known about the properties of C-fiber mediated network synaptic responses in the spinal cord dorsal *** the present presentation,multi-electrode array（Med64 system,Alpha-Med Sciences Co.,Ltd.） recordings were made on acute spinal cord slices attached with dorsal root obtained from adult *** stimulation at the lateral entry zone of the dorsal root via bipolar rectangular pulse（30-300 microA,100 microseconds） evoked network synaptic responses of field postsynaptic potentials（fEPSPs） which were mainly confined to the superficial layers of the dorsal *** evoked network fEPSPs were mostly with an initial positive-going fast response followed by a small and slow negative *** conduction velocity（CV） of the signal conveyed from stimulation site to the recording site was about（0.36±0.04） m/s which corresponded to the CV of primary nociceptive *** network responses were intensity-dependent and could be eliminated by perfusion with *** magnesium-low calcium and ***,but not AP5,suggesting involvement of both pre-synaptic transmitter release and postsynaptic activation of glutamate non-NMDA receptors in production of network *** further confirm whether the network synaptic responses are mediated by primary Cfibers, multi-electrode array recordings were also made on spinal cord slices obtained from adult rats previously receiving subcutaneous injection of capsaicin（50 mg/kg.b.w.） on postnatal day 2 by which more than 95%of primary afferent Cfibers are believed to be destructed with *** the postnatal capsaicin-treated rats,network synaptic responses in superficial layers of the spinal cord dorsal horn were significantly decreased not only in number but also in amplitude of the fEPS

关键词： multi-electrode array spinal cord field postsynaptic potentials(fEPSPs) C-fibers

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Plastic change of network synaptic responses in dentate gyrus by persistent pain experience in rats:a multi-electrode array recording

Plastic change of network synaptic responses in dentate gyru...

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中国神经科学学会第四次会员代表大会暨第七届全国学术会议

作者： Xiao-Yan ZHAO~1,Zhen LI~2,Hua LI~1,Ying CHANG~1,Ying HE~1,Jun CHEN~(1,2) 1 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China 2 Institute for Biomedical Sciences of Pain,Tangdu Hospital,Fourth Military Medical University,Xi''an 710038,China

＜正＞Pain is a multi-dimentional experience consisting of sensory-discriminative,affective-motivational,and cognitive -evaluative *** the present presentation,we report how persistent inflammatory pain on one hindpaw can cause plastic changes in network synaptic responses in the dentate gyrus（DG） obtained by multi-electrode array（Med64 system,Alpha-Med Sciences Co.,Ltd.） recordings on acute hippocampal slices of rats receiving subcutaneous injection of bee venom which mimics honeybee sting on *** naive or saline-control rats,electrical test stimulation（40-200μA） at molecular layer of the DG where the perforant path fibers terminate could evoke widespread network synaptic responses shown as field excitatory postsynaptic potentials（fEPSPs）.The evoked network fEPSPs were mostly confined to the region of DG spatially with either positive- or negative-going fast PS wave followed by a small and slow opposite-going *** network responses were intensity-dependent and could be eliminated by perfusion with TTX,high magnesiumlow calcium and CNQX,respectively,but not AP5,suggesting involvement of both pre-synaptic transmitter release and postsynaptic activation of glutamate non-NMDA receptors in production of network ***,in rats suffering from persistent pain stimulation,the second small and slow wave of the evoked network fEPSPs was becoming sharp and bigger in amplitude which were becoming more sensitive to AP5 and bicuculline than naive and saline-control rats, suggesting involvement of glutamate NMDA and GAB AA receptors in the change of *** long-term potentiation of network synaptic responses was also significantly enhanced by persistent pain *** together,the present results add a new line of evidence showing strong impact of persistent pain experience on the network synaptic functions of the hippocampal formation.

关键词： Pain network synaptic responses dentate gyrus rat fEPSPs

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新型农村合作医疗与城镇居民医疗保险统筹管理的两种运行模式效果对比分析

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中国卫生经济 2011年第5期30卷 34-37页

作者：陈治水高广颖梁民琳郭欣欣首都医科大学卫生管理与教育学院北京100069 黑龙江省卫生厅农村卫生管理处哈尔滨150090

文章调查研究了黑龙江省两个市县新型农村合作医疗和城镇居民医疗保险统一管理的两种经办模式,从运行方案、主管方式和运行效果等方面进行比较,分析基金筹集、基金使用、门诊及住院补偿、资金流向和住院率等方面的差异,分析了由卫生部... 详细信息

文章调查研究了黑龙江省两个市县新型农村合作医疗和城镇居民医疗保险统一管理的两种经办模式,从运行方案、主管方式和运行效果等方面进行比较,分析基金筹集、基金使用、门诊及住院补偿、资金流向和住院率等方面的差异,分析了由卫生部门主管的一些明显优势,并提出城乡居民医疗保险统筹过程中"三方受益"的基本原则。

关键词：新型农村合作医疗城镇居民医疗保险城乡统筹运行效果

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《解读生死》的解读

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中国医学伦理学 2009年第4期22卷 77-78页

作者：张艳清张旭平首都医科大学哲学与社会科学系北京100069

医学因其"仁术"的特点,使得生命教育有着更重要的意义。在《解读生死》教学专题感想的基础上分析生命教育在医学研究生综合素质培养中的重要性。教育不仅要直面生命,更要助人体验生命的意义和实现生命的超越。对医学生来说,构建生命化... 详细信息

医学因其"仁术"的特点,使得生命教育有着更重要的意义。在《解读生死》教学专题感想的基础上分析生命教育在医学研究生综合素质培养中的重要性。教育不仅要直面生命,更要助人体验生命的意义和实现生命的超越。对医学生来说,构建生命化课堂教学也是提高教学效果、培养其人文关怀的重要选择,应该引起关注。

关键词：医学研究生生死生命教育

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Antinociceptive effects of rapamycin on nociception and pain hypersensitivity:roles of mTOR signaling pathway?

Antinociceptive effects of rapamycin on nociception and pain...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Dan LV~1,Yan WANG~2,Chun-Li LI~2,Fan YANG~2,Jun CHEN~(1,2,*) 1 Institute for Biomedical Sciences of Pain,Capital Medical University,beijing 100069,China 2 Institute for Biomedical Sciences of Pain and Institute for Functional Brain Disorders,Tangdu Hospital,Fourth Military Medical University,Xi''an 710038,China

＜正＞Objective Mammalian target of rapamycin（mTOR） is a key regulator of mRNA translation which can be modified in a wide range of pathological *** studies indicated that mTOR was likely to be involved in the pathological process of pain hypersensitivity and neuronal *** the present report,we evaluate whether rapamycin is effective in antinociception and anti-hyperalgesia or anti-allodynia caused by subcutaneous injection of whole bee venom（BV）.Methods Rapamycin,a mTOR inhibitor,was administrered by intrathecal（i.t.） administration and intraplantar（***.） administration in conscious *** behavioral tests,persistent spontaneous nociceptive reflex was quantified by counting the number of paw flinches,heat hyperalgesia was quantified by measuring paw withdrawal thermal latency（PWTL） to radiant heat and mechanical hyperalgesia was quantified by measuring paw withdrawal threshold （PWMT） to von Frey *** *** of rapamycin（0.125,1.25 and 12.5μg） resulted in a doserelated suppression of the BV-induced persistent paw flinches,heat hypersensitivity,and mirror-image heat（MIH） hypersensitivity,but without any effectiveness on mechanical ***,*** of rapamycin （0.125,1.25 and 12.5μg） resulted in a dose-related suppression of the BV-induced persistent paw flinches,heat hypersensitivity,and mirror-image heat（MIH） hypersensitivity,but without any effectiveness on mechanical *** mTOR signaling pathway might be involved in pathophysiological processing associated with BV-induced nociception and inflammatory pain at both spinal and peripheral levels.

关键词： mammalian target of rapamycin(mTOR) persistent nociception pain hypersensitivity bee venom test

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The inhibition of dopamine on the colorectal motility is predominantly mediated by D1 and β3 receptors in normal and stress rats

The inhibition of dopamine on the colorectal motility is pre...

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中国生理学会第23届全国会员代表大会暨生理学学术大会

作者： Hua GUO,Yun LI,Jing-Dong XU,Xiao-Hui ZHANG,Li-Sheng LI,Yue ZHANG,Li-Fei ZHENG,Xin-Liang MI, Xiao-Yan FENG,Zhang-Feng DOU,Tuo JI,Xiao-Feng LI,Jin-Xia ZHU~* Department of Physiology,School of Basic Medical Sciences,Capital Medical University,beijing 100069,China

Colorectum manifests different in structure,function and accessibility to the diseases,such as inflammation, ulcer,even ***(DA) has been reported to involve in the regulation of colonic ***,few studies have clearly addressed the innervation and spontaneous motiliy regulation mechanism of DA on distal colon (DC).The present study demonstrated that the immune histofluorescence of D,D receptors,β,β andβ adrenoceptors were all found in the smooth muscle layer in the rat ***,D,β andβ were stronger than D andβ.DA(≥3.0×10mol/L)inhibited DC spontaneous motility[motility index(358±50) mg×1 min,frequency (1.06±0.08) Hz]in dose dependent manner(EC,1.0×10 mol/L).This DA-induced inhibition of colonic motility was blocked by pretreatment withβ adrenoceptor antagonist cyanopindolol(7.5×10 mol/L) mainly,D receptor antagonist SCH23390(1.0×10 mol/L) andβ adrenoceptor antagonist atenolol(1.0×10 mol/L) separately,otherwise D receptor antagonist sulpiride(1.0×10 mol/L) orβ adrenoceptor antagonist ICI-118551(1.0×10 mol/L) had no the blockage ***,pretreatments of D and D receptor antagonists made the spontaneous motility stronger *** more,in acute cold-restraint stress(CRS) conditions,the stronger spontaneous motility of DC could also be markedly inhibited by DA(≥3.0×10 mol/L).Then the result of western blot showed that expressions of only D,β and mainlyβ receptors,not D orβ receptors enhanced in the layer of CRS rats’ DC smooth *** conclusion,DA modulates DC motility in normal and stress rats mainly with D andβ *** alterations of DA-inhibited colonic motility and the increased expressions of D,β andβ receptors in rat DC smooth muscle under CRS condition indicates that DA and it’s receptor system play an important role in the regulation of physiologic and pathophysiologic process of gastrointestinal tract in the stress condition.

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网络信息时代电子病历的隐私保护研究

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中国卫生法制 2011年第6期19卷 60-62页

作者：关延风马骋宇山东省烟台市海港医院烟台264002 首都医科大学卫生管理与教育学院北京100069

与传统纸质病历相比,电子病历信息的存储和共享的范围将进一步扩大,但同时也为隐私信息的暴露提供捷径。本文通过分析我国电子病历隐私保护现状及存在的问题,并对比发展较好的其他国家电子病历隐私保护历程,提出我国进一步完善电子病历... 详细信息

与传统纸质病历相比,电子病历信息的存储和共享的范围将进一步扩大,但同时也为隐私信息的暴露提供捷径。本文通过分析我国电子病历隐私保护现状及存在的问题,并对比发展较好的其他国家电子病历隐私保护历程,提出我国进一步完善电子病历隐私保护的建议。

关键词：隐私保护电子病历

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基于关联维数和最大Lyapunov指数的运动HRV信号的研究

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生物医学工程研究 2009年第3期28卷 188-192页

作者：李霞康天良全海英田新首都医科大学生物医学工程学院仪器学系北京100069

采用非线性方法研究了应激条件下心血管系统的调节功能状况。以30例中老年高血压患者和30例年轻健康者作为研究对象,首先采集阶梯试验中的动态心电信号,通过信号预处理获得运动心率变异性(exercise heart rate variability,EHRV),然后... 详细信息

采用非线性方法研究了应激条件下心血管系统的调节功能状况。以30例中老年高血压患者和30例年轻健康者作为研究对象,首先采集阶梯试验中的动态心电信号,通过信号预处理获得运动心率变异性(exercise heart rate variability,EHRV),然后从运动前静息期和运动后恢复期EHRV中提取两个非线性指标——关联维数和最大Lyapunov指数,最后进行了统计分析。发现在两组测试对象中,除了运动前的最大Lyapunov指数,其余三个指标均有显著差异性,可有效区分高血压患者和健康者两组人群。对于健康者,运动前与运动后的关联维数、最大Lyapunov指数分别都具有显著差异性,而高血压患者运动前后的两个非线性指标都不具有显著差异性。说明健康者的交感神经系统在应激状态下能作出适应性的调节,而高血压患者此调节功能不明显。

关键词：关联维数最大Lyapunov指数 EHRV 心血管调节功能阶梯试验

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人羊膜间充质干细胞的分离及分化潜能的研究

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生物医学工程与临床 2010年第1期14卷 15-19页

作者：朴正福丁淑芹张海燕小林护加茂功樱川宣男李宁首都医科大学附属北京佑安医院北京市卫生局肝炎研究所日本生物资源应用研究所

目的建立人羊膜间充质干细胞(hAMC)的分离培养方法及探讨其在再生医学中的潜在应用性。方法取38～40周孕妇剖宫产术后人羊膜,用多种酶消化法制备hAMC悬浮液,并用免疫细胞化学方法探讨其多分化潜能如hAMC的神经及肝脏发生相关蛋白的表达... 详细信息

目的建立人羊膜间充质干细胞(hAMC)的分离培养方法及探讨其在再生医学中的潜在应用性。方法取38～40周孕妇剖宫产术后人羊膜,用多种酶消化法制备hAMC悬浮液,并用免疫细胞化学方法探讨其多分化潜能如hAMC的神经及肝脏发生相关蛋白的表达;荧光激活细胞仪分离法(FACS)检测主要组织相容性复合体(MHC)家族分子,同时探讨其致瘤性。结果从人羊膜中分离出高纯度hAMC,并摸索出其最适合的培养条件;细胞成细长梭形或星形,胞质丰富,细胞核呈圆形,1～3个核仁,易贴壁,生长繁殖快,在体外可连续培养20代以上。免疫细胞化学结果显示:神经发生相关蛋白(Musashi-1、Nestin、Vimentin)阳性及肝脏发生相关蛋白[Flt-1、Integrinβ1、HNF(3β、4α、1α)、C/EBPα、Albumin、AAT]等阳性;人MHC ClassⅡ阴性,人MHC ClassⅠ弱阳性;癌化实验显示hAMC无致瘤性。结论人羊膜中分离提纯的hAMC中至少包含向神经及肝脏细胞分化的干细胞样的细胞群,具有低免疫原性、无致瘤性等特点。这些结果支持hAMC作为新的、安全的细胞来源可用于再生医学研究。

关键词：羊膜羊膜间充质干细胞生物学特性再生医学

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