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Foodborne toxin Aflatoxin B_(1)induced glomerular podocyte inflammation through proteolysis of RelA,downregulation of miR-9 and CXCR4/TXNIP/NLRP3 pathway

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Food Science and Human Wellness 2024年第4期13卷 2289-2309页

作者： Jie Zhang Shuang Yang baocai Xu Zihui Qin Xinyi Guo Ben Wei Qinghua Wu Kamil Kuca Tushuai Li Wenda Wu School of Biology and Food Engineering Changshu Institute of TechnologySuzhou 215500China Center for Clinical Mass Spectrometry School of Pharmaceutical SciencesSoochow UniversitySuzhou 215123China School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural UniversityNanjing 210095China Department of Chemistry Faculty of ScienceUniversity of Hradec KraloveHradec Kralove 50003Czech Republic College of Life Science Yangtze UniversityJingzhou 434025China Wuxi Medical College Jiangnan UniversityWuxi 214122China Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch Wuxi 214013China

Aflatoxin B_(1)(AFB_(1))is a naturally-occurring mycotoxin and recognized as the most toxic foodborne toxin,particularly causing damages to *** podocytes are terminally differentiated epithelial ***_(1)induces podocyte inflammation,proteinuria and renal *** the mechanism of AFB_(1)-induced podocyte inflammation and murine kidney dysfunction,we detected that AFB_(1)increased ubiquitindependent degradation of the transcription factor RelA through enhanced interaction of RelA with E3 ubiquitin ligase tripartite motif containing 7(TRIM7)in mouse podocyte clone-5(MPC-5)and mouse *** of RelA resulted in decreasing microRNA-9(miR-9)and activating the chemokine receptor 4(CXCR4),thioredoxin interacting protein(TXNIP),and NOD-like receptor pyrin domain-containing 3(NLRP3)signaling axis(CXCR4/TXNIP/NLRP3 pathway),leading to podocyte *** also determined that downregulation of miR-9 led to CXCR4 expression and the downstream TXNIP/NLRP3 pathway *** of miR-9 or deletion of CXCR4 suppressed AFB_(1)-induced CXCR4/TXNIP/NLRP3 pathway,resulting in alleviating podocyte inflammation and kidney *** findings indicated that ubiquitin-dependent proteolysis of RelA,downregulation of miR-9,and activation of CXCR4/TXNIP/NLRP3 pathway played an essential role in AFB_(1)-induced glomerular podocyte *** study revealed a novel mechanism,via RelA,for the control of AFB_(1)’s nephrotoxicity,leading to an effective protection of food safety and public health.

关键词： Aflatoxin B_(1) Podocyte inflammation miRNA-9 Chemokine(C-X-C motif)receptor 4 RelA ubiquitin-dependent degradation

Pan-cancer analysis identifies RNA helicase DDX1 as a prognostic marker

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Phenomics 2022年第1期2卷 33-49页

作者： baocai Gao Xiangnan Li Shujie Li Sen Wang Jiaxue Wu Jixi Li State Key Laboratory of Genetic Engineering School of Life SciencesMOE Engineering Research Center of Gene TechnologyShanghai Engineering Research Center of Industrial MicroorganismsFudan UniversityShanghai 200438China Kunming Institute of Physics Kunming 650223China

The DEAD-box RNA helicase(DDX)family plays a critical role in the growth and development of multiple ***1 is involved in mRNA/rRNA processing and mature,virus replication and transcription,hormone metabolism,tumo-rigenesis,and tumor ***,how DDX1 functions in various cancers remains ***,we explored the potential oncogenic roles of DDX1 across 33 tumors with The Cancer Genome Atlas(TCGA)and the Genotype-Tissue Expression(GTEx)***1 is highly expressed in breast cancer(BRCA),cholangiocarcinoma(CHOL),and colon adenocarcinoma(COAD),but it is lowly expressed in renal cancers,including kidney renal clear cell carcinoma(KIRC),kidney chromophobe(KICH),and kidney renal papillary cell carcinoma(KIRP).Low expression of DDX1 in KIRC is cor-related with a good prognosis of overall survival(OS)and disease-free survival(DFS).Highly expressed DDX1 is linked to a poor prognosis of OS for adrenocortical carcinoma(ACC),bladder urothelial carcinoma(BLCA),KICH,and liver hepatocellular carcinoma(LIHC).Also,the residue Ser481 of DDX1 had an enhanced phosphorylation level in BRCA and ovarian cancer(OV)but decreased in *** infiltration analysis exhibited that DDX1 expression affected CD8+T cells,and it was significantly associated with MSI(microsatellite instability),TMB(tumor mutational burden),and ICT(immune checkpoint blockade therapy)in *** addition,the depletion of DDX1 dramatically affected the cell viability of human tumor-derived cell ***1 could affect the DNA repair pathway and the RNA transport/DNA replication processes during tumorigenesis by analyzing the CancerSEA ***,our pan-cancer analysis revealed that DDX1 had complicated impacts on different cancers and might act as a prognostic marker for cancers such as renal cancer.

关键词： RNA helicase DDX1 Pan-cancer analysis Survival analysis Prognostic marker

Does not hUTP14a promoter form a regulation feedback loop with P53?

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Chinese Journal of Cancer Research 2014年第2期26卷 159-165页

作者： Jingyi Zhang Yafei Guo Xiaojuan Du baocai Xing Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Department of Hepatic Biliary & Pancreatic Surgery IPeking University Cancer Hospital & Institute Department of Cell Biology Peking University Health Science Center

Objective： We previously found that hUTP14a binds P53 and promotes P53 degradation. However, if hUTP14a is a downstream gene of P53 remains to be determined. This study aimed to identify the promoter of h UTP14a and investigate if h UTP14a is regulated by P53. Methods： The hUTPI4a promoter region was cloned into pGL3-Basic-luciferase reporter plasmid to get pGL3-hUTP14a-luc. The reporter plasmid was transfected into 293T cells and luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Putative transcription factors were identified through searching Matlnspector Professional and Algorismica i Genetica databases. Either pGL3-hUTPI4aluc or p21 promoter reporter plasmid was co-transfected with increasing dose of p53 plasmid, and luciferase activity was evaluated. A series of deletion constructs of pGL3-hUTP14a-luc were constructed and minimal promoter region of hUTP14a was determined. Differences of the lnciferase activities between different groups were assessed by statistical analysis. Results： The hUTP14a gene promoter reporter construct was correctly cloned and was demonstrated to possess promoter activity. The transcription of hUTP14a was not regulated by P53. The minimal promoter region of h UTP14a gene is located between -203 to -100 of the transcription initiation site. Conclusion： Unlike other P53-interacting proteins such as MDM2, Pirh2 and Cop I which promote P53 degradation and whose transcriptions are regulated by P53, does not hUTP14a transcription form a regulation feedback loop with P 53.

关键词： hUTP 14a luciferase activity promoter P5 3 transcription factor

Engineering Shewanella-re duce d graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles

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Journal of Materials Science & Technology 2024年第1期168卷 50-61页

作者： baocai Zhang Shulin Shao Huan Yu Qijing Liu Sicheng Shi Qingyuan Wu Zijie Wu Yiyu Feng Yifei Wang Xi Sun Deguang Wu Feng Li Hao Song Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing 100029China School of Materials Science and Engineering Tianjin UniversityTianjin 300350China College of Biological Engineering Tianjin Agricultural UniversityTianjin 300384China Department of Brewing Engineering Moutai InstituteLuban AveRenhuai 564507China

Biosynthesizing Au nanoparticles(AuNPs)from gold-bearing scraps provides a sustainable method to meet the urgent demand for ***,it remains challenging to efficiently biosynthesize AuNPs of which the diameter is less than 10 nm from a trace amount of Au^(3+)concentration at the level of tens ***,we constructed an exoelectrogenic cell(eCell)-conductive reduced-graphene-oxide aero-gel(rGA)biohybrid by assembling Shewanella sp.S1(SS1)as living biocatalyst and rGA as conductive ad-sorbent,in which Au^(3+)at trace concentrations would be enriched by the adsorption of rGA and reduced to AuNPs through the extracellular electron transfer(EET)of *** regulate the size of the synthe-sized AuNPs to 10 nm,the strain SS1 was engineered to enhance its EET,resulting in strain RS2(pYYD-P tac-ribADEHC&pHG13-P_(bad)-omcC in SS1).Strain RS2 was further assembled with rGA to construct the RS2-rGA biohybrid,which could synthesize AuNPs with the size of 7.62±2.82 nm from 60 ppm Au^(3+)*** eCell-rGA biohybrid integrated Au^(3+)adsorption and reduction,which enabled AuNPs biosyn-thesis from a trace amount of Au^(3+).Thus,the required Au^(3+)ions concentration was reduced by one or two orders of magnitude compared with conventional methods of AuNPs *** work devel-oped an AuNPs size regulation technology via engineering eCell’s EET with synthetic biology methods,providing a feasible approach to synthesize AuNPs with controllable size from trace level of gold ions.

关键词： Au nanoparticles Exoelectrogenic cell(e-Cell) Shewanella Synthesis biology Extracellular electron transfer Reduced graphene oxide aerogel

Perfection and Application of GeologicModel During Oilfield Development

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China Oil & Gas 1997年第2期4卷 78-79页

作者： Chao Huaqing Fang baocai Chief Geologist Daqing Petroleum Administration Senior Enigineer Daqing Petroleum Administration

Lasaxing Oilfield, located at the north part of Daqing placanticline in the central uplift area of the northern Songliao Basin, is a large multi-zone oilfield of fluvial-delta sedimentary continental *** was made in different development stages aiming at different development layers, and knowledge was gradually deepened from high-permeability to low-permeability and first thick layers, then to thin layers and finally to untabulated layers. After the overall development of Lasaxing Oilfield, oil production reached 50 million tons in 1976, which has been maintained for 21 years and still can be further maintained for a longer period. This proves that the established and perfected geologic model is suitable.

关键词： Geologic model Well Dattern Annlication

Three-Dimensional N-Doped Carbon Nanotube/Graphene Composite Aerogel Anode to Develop High-Power Microbial Fuel Cell

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Energy & Environmental Materials 2023年第3期6卷 161-169页

作者： Shixuan Jin Yiyu Feng Jichao Jia Fulai Zhao Zijie Wu Peng Long Feng Li Huitao Yu Chi Yang Qijing Liu baocai Zhang Hao Song Wei Feng School of Material Science and Engineering Tianjin UniversityTianjin 300350China School of Chemical Engineering and Technology Tianjin UniversityTianjin 300350China

Optimizing the structure of electrode materials is one of the most effective strategies for designing high-power microbial fuel cells(MFCs).However,electrode materials currently suffer from a series of shortcomings that limit the output of MFCs,such as high intrinsic resistance,poor electrolyte wettability,and low microbial load ***,a three-dimensional(3D)nitrogen-doped multiwalled carbon nanotube/graphene(N-MWCNT/GA)composite aerogel is synthesized as the anode for *** nitrogen-doped GA,MWCNT/GA,and N-MWCNT/GA,the macroporous hydrophilic N-MWCNT/GA electrode with an average pore size of 4.24μm enables high-density loading of the microbes and facilitates extracellular electron transfer with low intrinsic ***,the hydrophilic surface of N-MWCNT can generate high charge mobility,enabling a high-power output performance of the *** consequence,the MFC system based on N-MWCNT/GA anode exhibits a peak power density and output voltage of 2977.8 mW m^(−2)and 0.654 V,which are 1.83 times and 16.3%higher than those obtained with MWCNT/GA,*** results demonstrate that 3D N-MWCNT/GA anodes can be developed for high-power MFCs in different environments by optimizing their chemical and microstructures.

关键词： anode graphene aerogel microbial fuel cell N-doped carbon nanotube

Development Status of Daqing Oilfields In 1998

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China Oil & Gas 1999年第1期6卷 36-37页

作者： Fang baocai(Daqing Petroleum Administration)

A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides

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Plant Communications 2021年第2期2卷 102-110页

作者： Yao Cai baocai Zhang Liyuan Liang Sen Wang Lanjun Zhang Liang Wang Hong-Liang Cui Yihua Zhou Deqiang Wang College of Instrumentation and Electrical Engineering Jilin UniversityChangchunJilin 130016China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Chongqing Key Laboratory of Multi-scale Manufacturing Technology Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China University of the Chinese Academy of Sciences Beijing 100049China

Polysaccharides are important biomacromolecules existing in all plants,most of which are integrated into a fibrillar structure called the cell *** the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility,our knowledge of cell wall architecture and function is greatly ***,we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification *** designed a solid-state nanopore sensor supported by a free-standing SiNx membrane in fluidic *** device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/mL and discriminate xylans with hyperacetylated and unacetylated *** further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot *** the data for categorizing polysaccharide mixtures,our study establishes a single-molecule platformfor polysaccharide analysis,opening a new avenue for understanding cell wall structures,and expanding polysaccharide applications.

关键词： solid-state nanopore single-molecule polysaccharides acetylation ion current

Multi-omics Analysis of Primary Cell Culture Models Reveals Genetic and Epigenetic Basis of Intratumoral Phenotypic Diversity

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Genomics, Proteomics & Bioinformatics 2019年第6期17卷 576-589页

作者： Sixue Liu Zuyu Yang Guanghao Li Chunyan Li Yanting Luo Qiang Gong Xin Wu Tao Li Zhiqian Zhang baocai Xing Xiaolan Xu Xuemei Lu CAS Key Laboratory of Genomics and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Invasive Pathogens Laboratory Institute of Environmental Science and ResearchPorirua 5022WellingtonNew Zealand Department of Cell Biology Key Laboratory of Carcinogenesis and Translational ResearchCenter for Molecular and Translational MedicinePeking University Cancer Hospital and InstituteBeijing 100142China Department of Hepatobiliary Surgery I Peking University Cancer Hospital and InstituteBeijing 100142China National Key Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China CAS Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunming 650223China

Uncovering the functionally essential variations related to tumorigenesis and tumor progression from cancer genomics data is still challenging due to the genetic diversity among patients,and extensive inter-and intra-tumoral heterogeneity at different levels of gene expression regulation,including but not limited to the genomic, epigenomic, and transcriptional levels. To minimize the impact of germline genetic heterogeneities, in this study, we establish multiple primary cultures from the primary and recurrent tumors of a single patient with hepatocellular carcinoma(HCC). Multiomics sequencing was performed for these cultures that encompass the diversity of tumor cells from the same patient. Variations in the genome sequence, epigenetic modification, and gene expression are used to infer the phylogenetic relationships of these cell cultures. We find the discrepancy among the relationships revealed by single nucleotide variations(SNVs) and transcriptional/epigenomic profiles from the cell cultures. We fail to find overlap between sample-specific mutated genes and differentially expressed genes(DEGs), suggesting that most of the heterogeneous SNVs among tumor stages or lineages of the patient are functionally insignificant. Moreover, copy number alterations(CNAs) and DNA methylation variation within gene bodies, rather than promoters, are significantly correlated with gene expression variability among these cell cultures. Pathway analysis of CNA/DNA methylation-related genes indicates that a single cell clone from the recurrent tumor exhibits distinct cellular characteristics and tumorigenicity, and such an observation is further confirmed by cellular experiments both in vitro and in vivo. Our systematic analysis reveals that CNAs and epigenomic changes, rather than SNVs, are more likely to contribute to the phenotypic diversity among subpopulations in the tumor. These findings suggest that new therapeutic strategies targeting gene dosage and epigenetic modification s

关键词： Intra-individual tumor heterogeneity Parallel primary culture Multi-omics Functional phenotypic diversity