Engineering Shewanella-re duce d graphene oxide aerogel biohybrid to efficiently synthesize Au nanoparticles
作者机构：Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education)Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin)School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China Beijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical TechnologyBeijing 100029China School of Materials Science and EngineeringTianjin UniversityTianjin 300350China College of Biological EngineeringTianjin Agricultural UniversityTianjin 300384China Department of Brewing EngineeringMoutai InstituteLuban AveRenhuai 564507China
出 版 物：《Journal of Materials Science & Technology》 (材料科学技术（英文版）)
年 卷 期：2024年第168卷第1期
页 面：50-61页
核心收录：
学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术]
基 金：supported by the National Key Research and Development Program of China(No.2018YFA0901300) the Na-tional Natural Science Foundation of China(Nos.NSFC 32071411,NSFC 32001034,and NSFC 31701569) the Young Science and Tech-nology Talents Growth Project of Education Department of Guizhou Province(No.KY445) Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation,China National Light Indus-try(No.2021JJ013)
主 题：Au nanoparticles Exoelectrogenic cell(e-Cell) Shewanella Synthesis biology Extracellular electron transfer Reduced graphene oxide aerogel
摘 要：Biosynthesizing Au nanoparticles(AuNPs)from gold-bearing scraps provides a sustainable method to meet the urgent demand for ***,it remains challenging to efficiently biosynthesize AuNPs of which the diameter is less than 10 nm from a trace amount of Au^(3+)concentration at the level of tens ***,we constructed an exoelectrogenic cell(eCell)-conductive reduced-graphene-oxide aero-gel(rGA)biohybrid by assembling Shewanella sp.S1(SS1)as living biocatalyst and rGA as conductive ad-sorbent,in which Au^(3+)at trace concentrations would be enriched by the adsorption of rGA and reduced to AuNPs through the extracellular electron transfer(EET)of *** regulate the size of the synthe-sized AuNPs to 10 nm,the strain SS1 was engineered to enhance its EET,resulting in strain RS2(pYYD-P tac-ribADEHC&pHG13-P_(bad)-omcC in SS1).Strain RS2 was further assembled with rGA to construct the RS2-rGA biohybrid,which could synthesize AuNPs with the size of 7.62±2.82 nm from 60 ppm Au^(3+)*** eCell-rGA biohybrid integrated Au^(3+)adsorption and reduction,which enabled AuNPs biosyn-thesis from a trace amount of Au^(3+).Thus,the required Au^(3+)ions concentration was reduced by one or two orders of magnitude compared with conventional methods of AuNPs *** work devel-oped an AuNPs size regulation technology via engineering eCell’s EET with synthetic biology methods,providing a feasible approach to synthesize AuNPs with controllable size from trace level of gold ions.
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