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Evaluation of entomopathogenic Bacillus sphaericus isolated from Lombok beach area against mosquito larvae

Asian Pacific Journal of Tropical Biomedicine 2016年第2期6卷 148-154页

作者： bambang fajar suryadi Bagyo Yanuwiadi Tri Ardyati Suharjono Suharjono Department of Biology Faculty of Mathematics Natural Sciences Mataram University Department of Biology Faculty of Mathematics Natural Sciences Brawijaya University

Objective: To isolate, characterize and evaluate toxicity of Bacillus sphaericus(B. sphaericus) from beach area of Lombok ***: Soil was collected from determined locations and suspended in sterile physiological saline water. After heat shock was applied, suspension was spread on NYSM agar medium. Colonies grown were then observed and isolated. Colony, cell morphology,and biochemical/physiological characteristics were tested and compared to B. sphaericus2362 as standard. Initial toxicity testing was done against three species of mosquito larvae(Culex quinquefasciatus, Anopheles aconitus and Aedes aegypti) and isolates that showed more than 50% larvae killing will be assayed to obtain LC_(50) and LC_(90)values within 48 *** technique were conducted to obtain 16 s r DNA amplicon for sequencing and to detect toxin-expressing genes(using multiplex PCR).Results: Twenty isolates of B. sphaericus have been collected from 20 determined locations and their characteristics were in agreement with standard B. sphaericus characteristics. Bioassay testing showed that four isolates(namely isolate MNT, SLG, TJL2 and PLG) were mildly toxic against all larvae. The rests were either low toxic or non-toxic at *** analysis showed that all four isolates were clustered with other known mildly and highly toxic strains. The multiplex PCR result showed four toxic isolates owned 1–2bands from Bin toxin genes and three bands from Mtx toxin genes, whereas 16 isolates with low to non-toxic characteristics showed only three bands from Mtx toxin ***: Four toxic isolates of B. sphaericus were isolated from beach area of Lombok Island. They showed mild toxicity against larvae of three mosquito species.

关键词： Bacillus sphaericus Lombok Island Mosquito larvae Culex quinquefasciatus Anopheles aconitus Aedes aegypti

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Development of Architectures for Internet Telerobotics Systems

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Journal of Bionic Engineering 2007年第4期4卷 291-297页

作者： Riyanto T. bambang School of Electrical Engineering and Informatics Bandung Institute of Technology Bandung 40132 Indonesia

This paper presents our experience in developing and implementing Internet telerobotics system. Internet telerobotics system refers to a robot system controlled and monitored remotely through the Internet. A robot manipulator with five degrees of freedom, called Mentor, is employed. Client-server architecture is chosen as a platform for our Internet telerobotics system. Three generations of telerobotics systems have evolved in this research. The first generation was based on CGI and two tiered architectures, where a client presents a Graphical User Interface to the user, and utilizes the user＇s data entry and actions to perform requests to robot server running on a different machine. The second generation was developed using Java. We also employ Java 3D for creating and manipulating 3D geometry of manipulator links, and for constructing the structures used in rendering that geometry, resulting in 3D robot movement simulation presented to the users （clients） through their web browser. Recent development in our Internet telerobotics includes object recognition through image captured by a camera, which poses challenging problem, giving the undeterministic latency of the Internet. The third generation is centered around the use of CORBA for development platform of distributed internet telerobotics system, aimed at distributing task of telerobotics system.

关键词： telerobotics manipulator Internet CGI Java Java 3D CORBA

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Supercritical water oxidation for the destruction of toxic organic wastewaters:A review

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Journal of Environmental Sciences 2007年第5期19卷 513-522页

作者： VERIANSYAH bambang KIM Jae-Duck Supercritical Fluid Research Laboratory Korea Institute of Science and Technology (KIST)-Department of Green Process and System EngineeringUniversity of Science and Technology (UST)39-1 Hawolgok-dongSeoungbuk-guSeoul 136-791Korea

The destruction of toxic organic wastewaters from munitions demilitarization and complex industrial chemical clearly becomes an overwhelming problem if left to conventional treatment processes. Two options, incineration and supercritical water oxidation （SCWO）, exist for the complete destruction of toxic organic wastewaters. Incinerator has associated problems such as very high cost and public resentment; on the other hand, SCWO has proved to be a very promising method for the treatment of many different wastewaters with extremely efficient organic waste destruction 99.99% with none of the emissions associated with incineration. In this review, the concepts of SCWO, result and present perspectives of application, and industrial status of SCWO are critically examined and discussed.

关键词： supercritical water oxidation toxic wastewater treatment SCWO industrial status

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SOFC composite electrolyte based on LSGM-8282 and zirconia or doped zirconia from zircon concentrate

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International Journal of Minerals,Metallurgy and Materials 2012年第9期19卷 863-871页

作者： Fitria Rahmawati bambang Prijamboedi Syoni Soepriyanto Ismunandar Inorganic and Physical Chemistry Research Group Faculty of Mathematics and Natural Sciences Institut Teknologi Bandung Jl. Ganesha 10 Bandung 40132 Indonesia Research Group of Solid State & Catalysis Chemistry Department Sebelas Maret University Jl. Ir. Sutami 36 A Kentingan Surakarta 57126 Indonesia Metallurgical Engineering Research Group Faculty of Mining and Petroleum Engineering Institut Teknologi Bandung Jl. Ganesha 10 Bandung 40132 Indonesia

The aim of this research is to study zirconia-based electrolyte materials to increase the commercial value of zircon concentrate as a side product of fin mining industries. Synthesis of CaO-Y2O3-ZrO2 （CYZ） and 8mol% Y2O3-ZrO2 （8YSZ） was carried out by solid state reaction. The result shows that ZrO2 presents in tetragonal phase. Doping of Y203 into ZrO2 allows a phase transformation from tetragonal into cubic structure with small percentage of monoclinic phase. Meanwhile, doping of CaO-Y2O3 allows a phase transformation into a single cubic phase. These phase transformations enhance the ionic conductivity of the material. Introduction of 10wt% of LSGM-8282 into CYZ （CYZ-L90：10） allows further improvement of inter-grain contact shown by SEM morphological analysis and leads to the enhancement of ionic conductivity.

关键词： solid oxide fuel cells (SOFC) solid electrolytes LSGM electrolyte zirconia phase transformations ionic conductivity

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Destruction of methylphosphonic acid in a supercritical water oxidation bench-scale double wall reactor

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Journal of Environmental Sciences 2011年第4期23卷 545-552页

作者： bambang Veriansyah Eun-Seok Song Jae-Duck Kim Supercritical Fluid Research Laboratory Korea Institute of Science and Technology (KIST) 39-1 Hawolgok-dong Seoungbuk-gu Seoul 136-791 Korea

The destruction of methylphosphonic acid （MPA）, a final product by hydrolysis/neutralization of organophosphorus agents such as sarin and VX （O-ethyl S-[2-（diisopropylamino）ethyl] methylphosphonothionate）, was investigated in a a bench-scale, continuous concentric vertical double wall reactor under supercritical water oxidation condition. The experiments were conducted at a temperature range of 450–600°C and a fixed pressure of 25 MPa. Hydrogen peroxide was used as an oxidant. The destruction efficiency （DE） was monitored by analyzing total organic carbon （TOC） and MPA concentrations using ion chromatography on the liquid effluent samples. The results showed that the DE of MPA up to 99.999% was achieved at a reaction temperature of 600°C, oxygen concentration of 113% storichiometric requirement, and reactor residence time of 8 sec. On the basis of the data derived from experiments, a global kinetic rate equation for the DE of MPA and DE of TOC were developed by nonlinear regression analysis. The model predictions agreed well with the experimental data.

关键词： supercritical water oxidation destruction efficiency methylphosphonic acid reaction kinetic nonlinear regression

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Hydrothermal decomposition of pentachlorophenol in subcritical and supercritical water with sodium hydroxide addition

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Journal of Environmental Sciences 2007年第6期19卷 663-666页

作者： PRABOWO Benedictus VERIANSYAH bambang KIM Jae-Duck Supercritical Fluid Research Laboratory Korea Institute of Science and Technology(KIST)-Department of Green Process and System EngineeringUniversity of Science and Technology(UST)39-1 Hawolgok-dongSeoungbuk-gu Seoul136-791Korea.

Hydrothermal decomposition of pentachlorophenol （PCP, C6HC150）, as the probable human carcinogen, was investigated in a tubular reactor under subcritical and supercritical water with sodium hydroxide （NaOH） addition. The experiments were conducted at a temperature range of 30（0-420℃ and a fixed pressure of 25 MPa, with a residence time that ranged from 10 s to 70 s. Under the reaction conditions, the initial PCP concentrations were varied from 0.25 to 1.39 mmol/L, and the NaOH concentrations were varied from 2.5 to 25 times of the concentrations of PCP. The result of this study showed that PCP conversion in supercritical water was highly dependent on the reaction temperature, residence time, and NaOH concentration. PCP conversion in subcritical water is, however, only dependent on reaction temperature. NaOH concentration and residence times were found to have little effect on PCP conversion in subcritical condition. It was found that NaOH concentration affected the dechlorinations of PCP in the supercritical water. The intermediates detected were proposed to be tetrachlorophenol and trichlorophenol, respectively.

关键词： pentachlorophenol hydrothermal decomposition supercritical water suberitical water

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Scanometry as microplate reader for high throughput method based on DPPH dry reagent for antioxidant assay

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Acta Pharmaceutica Sinica B 2017年第3期7卷 395-400页

作者： Mochammad Amrun Hidayat Aulia Fitri bambang Kuswandi Chemo and Biosensor Group Faculty of PharmacyUniversity of JemberJember 68121Indonesia

The stable chromogenic radical 1,1'-diphenyl-2-picrylhydrazyl(DPPH) solution was immobilized on the microwell plate as dry reagent to construct a simple antioxidant sensor. Then, a regular flatbed scanner was used as microplate reader to obtain analytical parameters for antioxidant assay using one-shot optical sensors as scanometry technique. Variables affecting the acquisition of the images were optimized and the analytical parameters are obtained from an area of the sensing zone inside microwell using the average luminosity of the sensing zone captured as the mean of red, green, and blue(RGB)value using Image J~program. By using this RGB value as sensor response, it is possible to determine antioxidant capacity in the range 1–25 ppm as gallic acid equivalent(GAE) with the response time of 9 min. The reproducibility of sensor was good(RSD<1%) with recovery at 93%–96%. The antioxidant sensor was applied to the plant extracts, such as sappan wood and Turmeric Rhizome. The results are good when compared to the same procedure using a UV/Vis spectrophotometer.

关键词： Scanometry High throughput DPPH Optical sensor Antioxidant

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Decomposition kinetics of dimethyl methylphospate(chemical agent simulant) by supercritical water oxidation

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Journal of Environmental Sciences 2006年第1期18卷 13-16页

作者： bambang VERIANSYAH Jae-Duck KIM Youn-Woo LEE Supercritical Fluid Research Laboratory Korea Institute of Science and Technology(KIST) 39-1 Hawolgok-dong Seoungbuk-gu Seoul 136-791 Korea. School of Chemical and Biological Engineering Seoul National University(SNU) San 56-1 Sillim-dong Gwanak-gu Seoul 151-744 Korea

Supercritical water oxidation （SCWO） has been drawing much attention due to effectively destroy a large variety of high-risk wastes resulting from munitions demilitarization and complex industrial chemical. An important design consideration in the development of supercritical water oxidation is the information of decomposition rate. In this paper, the decomposition rate of dimethyl methylphosphonate（DMMP）, which is similar to the nerve agent VX and GB（Sarin） in its structure, was investigated under SCWO conditions. The experiments were performed in an isothermal tubular reactor with a H2O2 as an oxidant. The reaction temperatures were ranged from 398 to 633℃ at a fixed pressure of 24 MPa. The conversion of DMMP was monitored by analyzing total organic carbon （TOC） on the liquid effluent samples. It is found that the oxidative decomposition of DMMP proceeded rapidly and a high TOC decomposition up to 99.99% was obtained within 11 s at 555℃. On the basis of data derived from experiments, a global kinetic equation for the decomposition of DMMP was developed. The model predictions agreed well with the experimental data.

关键词： supercritical water oxidation kinetics chemical agent DMMP

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Anti-angiogenic effect of Artocarpus heterophyllus seed methanolic extract in ex ovo chicken chorioallantoic membrane

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Asian Pacific Journal of Tropical Biomedicine 2017年第3期7卷 240-244页

作者： Swastika Oktavia Nastiti Wijayanti bambang Retnoaji Post Graduate Program Biology FacultyUniversitas Gadjah MadaYogyakartaIndonesia Department of Biology Science and Pharmacy FacultyMathla'ul Anwar UniversityBantenIndonesia Laboratory of Animal Physiology Faculty of BiologyUniversitas Gadjah MadaYogyakartaIndonesia Laboratory of Animal Structure and Development Faculty of BiologyUniversitas Gadjah MadaYogyakartaIndonesia

Objective: To examine the anti-angiogenic potential of Artocarpus heterophyllus(A. heterophyllus) seed extract in chicken chorioallantoic membrane(CAM).Methods: This study used chicken CAM ex ovo culture to examine the potential antiangiogenic activity of A. heterophyllus seed methanolic extract. Basic fibroblast growth factor was used to induce the ectopic formation of blood vessels on CAM treated with extract. Blood vessel number was assessed by macroscopic and microscopic observation,and compared and analyzed for all treatments and ***: Macroscopic observation revealed that a dose of 35 mg/m L of methanolic extract of A. heterophyllus seeds could inhibit basic fibroblast growth factor-induced angiogenesis by 61% in chicken CAM ex ovo culture. This concurred with microscopic observations on the histological structure of blood vessels, which indicated that extract treatment repressed the formation of new blood ***: This is the first study to report the anti-angiogenic effect of methanolic extract derived from A. heterophyllus seeds and its potential as a candidate for future anticancer therapy.

关键词： Angiogenesis Artocarpus heterophyllus CAM Ex ovo culture

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Structural Performance of Prefabricated Timber-Concrete Composite Floor Constructed Using Open Web Truss Joist Made of LVL Paraserianthes Falctaria

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Open Journal of Civil Engineering 2021年第4期11卷 434-450页

作者： Ali Awaludin Urwatul Wusqo Angga fajar Setiawan bambang Suhendro Suprapto Siwosukarto Achmad Basuki Adrian Leijten Department of Civil and Environmental Engineering Universitas Gadjah Mada Yogyakarta Indonesia Department of Civil Engineering Universitas Sebelas Maret Surakarta Indonesia Faculty of Built Environment Eindhoven University of Technology Eindhoven Netherlands

This paper investigates an open web truss joist (OWTJ) made of laminated veneer lumber (LVL) Paraserianthes falctaria as a prefabricated timber-concrete composite floor system. The four-point bending test showed that structural performances of the OWTJ, conventional composite floor (CCF), and prefabricated composite floor (PCF) were similar. Although composite action was not developed as no lateral deformation was observed at the shear connectors, installing a concrete slab above the OWTJ can slightly increase the ductility factor of the composite floor. Furthermore, a finite element model was developed, and the model proved to be suitable for simulating the structural performance of the composite floor.

关键词： LVL Sengon Open Web Truss Joist Pre-Fabricated Timber Floor

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