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Anticancer Activity of Rice Callus suspension Cultures from Aromatic Varieties and Metabolites Regulated in Treated Cancer Cell Lines

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Rice science 2024年第4期31卷 449-462,I0027-I0030页

作者： Anuradha KUMARI Wusirika ramakrishna Department of Biochemistry Central University of PunjabPunjab 151401India

Tissue culture techniques were used to produce large amounts of bioactive compounds with medicinal potential, overcoming space and time constraints for cancer prevention. Rice callus suspension cultures(RCsC) and seed extracts prepared from aromatic rice varieties were used to evaluate the cytotoxic impact on human colon and lung cancer cell lines, as well as a normal control cell line, using Taxol as a positive control. RCsC and seed extracts from two Indian aromatic rice varieties were applied at different concentrations to treat the cancer cell lines and normal lung fibroblasts over varying time intervals. Apoptosis was assessed in 1:5 dilutions of the A549 and HT-29 cell lines treated with RCsC for 72 h, using propidium iodide staining and flow cytometry. RCsC showed a more potent cytotoxic effect than seed extracts with minimal effect on the normal cell line, in contrast to Taxol. Confocal microscopy and flow cytometry further confirmed the apoptotic effect of RCsC. Gas chromatography-mass spectrometry-based metabolic profiling identified metabolites involved in cytotoxicity and highlighted altered pathways. RCsC is proposed as an alternative source for the development of novel anticancer drugs with reduced side effects.

关键词： anticancer cytotoxicity metabolite profiling plant tissue culture rice callus suspension culture secondary metabolite

Engineering the axial coordination of cobalt single atom catalysts for efficient photocatalytic hydrogen evolution

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Nano Research 2024年第6期17卷 5114-5121页

作者： Ning Kang Lingwen Liao Xue Zhang Zhen He Binlu Yu Jiahong Wang Yongquan Qu Paul KChu seeram ramakrishna Xue-Feng Yu Xin Wang Licheng Bai Materials Interfaces Center Shenzhen Institute of Advanced TechnologyChinese Academy of Sciences(CAS)Shenzhen 518055China Key Laboratory of Materials Physics Anhui Key Laboratory of Nanomaterials and NanotechnologyCAS Center for Excellence in NanoscienceInstitute of Solid State PhysicsChinese Academy of SciencesHefei 230031China Department of Chemistry City University of Hong KongKowloonHong Kong 999077China Hong Kong Branch of National Precious Metals Material Engineering Research Center(NPMM) City University of Hong KongKowloonHong Kong 999077China Key Laboratory of special Functional and smart Polymer Materials of Ministry of Industry and Information Technology School of Chemistry and Chemical EngineeringNorthwestern Polytechnical UniversityXi’an 710072China Department of Physics and Department of Materials science and Engineering City University of Hong KongKowloonHong Kong 999077China National University of singapore(NUs)Centre for Nanofibers and Nanotechnology Department of Mechanical EngineeringNational University of SingaporeSingapore 117542Singapore

Improving the catalytic activity of non-noble metal single atom catalysts(sACs)has attracted considerable attention in materials *** optimizing the local electronic structure of single atom can greatly improve their catalytic activity,it often involves in-plane modulation and requires high ***,we report a novel strategy to manipulate the local electronic structure of sACs via the modulation of axial Co-s bond anchored onto graphitic carbon nitride(C_(3)N_(4))at room temperature(RT).Each Co atom is bonded to four N atoms and one s atom(Co-(N,s)/C_(3)N_(4)).Owing to the greater electronegativity of s in the Co-s bond,the local electronic structure of the Co atoms is available to be controlled at a relatively moderate ***,when employed for the photocatalytic hydrogen evolution reaction,the adsorption energy of intermediate hydrogen(H*)on the Co atoms is remarkably *** the presence of the Co-(N,s)/C_(3)N_(4)sACs,the hydrogen evolution rates reach up to 10 mmol/(g·h),which is nearly 10 and 2.5 times greater than the rates in the presence of previously reported transition metal/C_(3)N_(4)and noble platinum nanoparticles(PtNPs)/C_(3)N_(4)catalysts,*** to the tailorable axial Co-s bond in the sAC,the local electronic structure of the Co atoms can be further optimized for other photocatalytic *** axial coordination engineering strategy is universal in catalyst designing and can be used for a variety of photocatalytic applications.

关键词： transition metal single-atom local electronic structure photocatalytic hydrogen evolution graphitic carbon nitride axial coordination environment

Basic research and industrial development status of suancai in Northeast China: History， microflora diversity， processing technology， nutrition and safety

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Food science and Human Wellness 2024年

作者： Qianqian Wang Jingbo Yang Yuqi Wang Meiru Xie Xiaoxi Qi Ramnarain ramakrishna Libo Liu Wenkui Guo Guofang Zhang Chun Li Key Laboratory of Dairy science， Ministry of Education， College of Food science， Northeast Agricultural University Department of Plant sciences， North Dakota state University Heilongjiang Institute of Green Food science

suancai has a lengthy history and a wide range of categories， which has some influence on the pickled diet culture around the world. suancai production is transitioning to a large-scale， standardized production due to the growth of the market. It has a unique flavor and is rich in nutrients， and its abundance of free amino acids， vitamins and phenolics has many positive effects on the human body. This review gives the types and history of suancai， as well as its impact on the world's pickled culture. The changes in nutritional composition and flavor of suancai during fermentation are summarized. It presented the production technology and influencing factors of the northeast suancai， examined the quality and safety issues in suancai， and put forth some ideas and opinions on the standardization development of the suancai industry. It also summarized the geographic distribution and flora diversity of pickles around the world. In order to provide some knowledge and guidance for the promotion of modern industrial production in the suancai industry.

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structural, mechanical and corrosion properties of CNT-304 stainless steel nanocomposites

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Progress in Natural science:Materials International 2019年第5期29卷 595-602页

作者： A.V.Radhamani Hon Chung Lau s.ramakrishna Department of Civil and Environmental Engineering National University of Singapore Department of Mechanical Engineering Centre for Nanofibers and Nanotechnology National University of Singapore

In this work, a systematic investigation was performed on the structural, mechanical and corrosion properties of CNT incorporated 304 stainless steel. Various concentrations of CNT from 0.5 to 4 wt% were incorporated into the 304 stainless steel matrix to investigate the feasibility of fabrication and enhancement of strength and other material properties. The fabrication of CNT-steel composite was achieved through a spark plasma sintering process at a sintering temperature of 800℃. Raman and morphological studies confirmed that the CNT structure was retained in the sintered pellets. Optimum performance was found at 0.5 wt% CNT giving a Vickers hardness of 351 Hv and compressive yield strength of 404 MPa which were 5.5 and 2.0 times, respectively, those of pristine steel. Corrosion studies with 3.5 wt% Na Cl solution revealed a slight increase in the corrosion rate for CNT dispersed samples.

关键词： Carbon nanotube 304-stainless steel spark plasma sintering Hardness Compression Corrosion

A simplified approach to assess seismic stability of tailings dams

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Journal of Rock Mechanics and Geotechnical Engineering 2018年第6期10卷 1082-1090页

作者： sanjay Nimbalkar V.s.ramakrishna Annapareddy Anindya Pain school of Civil and Environmental Engineering Faculty of Engineering and Information Technology University of Technology Sydney Geotechnical Engineering Group CSIR-Central Building Research Institute (CBRI)

In the zones of high seismic activity, tailings dam should be assessed for the stability against earthquake forces. In the present paper, a simplified method is proposed to compute the factor of safety of tailings dams. The strain-dependent dynamic properties are used to assess the stability of tailings dams under seismic conditions. The effect of foundation soil properties on the seismic stability of tailings dams is studied using the proposed method. For the given input parameters, the factor of safety for lowfrequency input motions is nearly 26% lower than that for high-frequency input excitations. The impedance ratio and the depth of foundation have significant effect on the seismic factor of safety of tailings dams. The results from the proposed method are well compared with the existing pseudo-static method of analysis. Tailings dams are vulnerable to damage for low-frequency input motions.

关键词： Tailings dam Horizontal slice method(HsM) Limit equilibrium strain-dependent property Pseudo-static method

synthesis of novel isoxazolyl bis-thiazolo[3,2-a]pyrimidines

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Chinese Chemical Letters 2012年第8期23卷 899-902页

作者： E.Rajanarendar s.ramakrishna K.Rama Murthy Department of Chemistry Kakatiya UniversityWarangalAP 506 009India

A new synthetic strategy for the synthesis of novel 3-（3-（3-methyl-4-nitroisoxazol-5-yl）-2-phenyl-1-（5,7-diaryl-7H-thia- zolo[3,2-a]pyrimidin-3-yl）propyl）-5,7-diaryl-7H-thiazolo[3,2-a]pyrimidines （7a-i） analogues is described. Reaction of 3-（2- （3-methyl-4-nitroisoxazole-5-yl）-l-phenylethyl）pentane-2,4-dione （3） with two moles of thiourea in presence of iodine and CuO afforded 4-（1-（2-aminothiazol-4-yl）-3-（3-methyl-4-nitroisoxazol-5-yl）-2-aryl propylthiazol-2-amine （5）. Compound 5 on reaction with two moles of chalcone （6） furnished novel 3-（3-（3-methyl-4-nitroisoxazol-5-yl）-2-phenyl-1-（5,7-diaryl-7H-thiazolo[3,2- a]pyrimidin-3-yl）propyl）-5,7-diaryl-7H-thiazolo[3,2-a ]pyrimidines （Ta-i）.

关键词： Isoxazolyl bis-thiazoles Isoxazolyl bis-thiazolo[3,2-a]pyrimidines New synthetic strategy

Dynamic Responses of Atmospheric Carbon Dioxide Concentration to Global Temperature Changes between 1850 and 2010

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Advances in Atmospheric sciences 2016年第2期33卷 247-258页

作者： Weile WANG ramakrishna NEMANI Department of science and Environmental Policy California State University at Monterey Bay Earth science Division NASA Ames Research Center NAsA Advanced supercomputing Division NASA Ames Research Center

Changes in Earth＇s temperature have significant impacts on the global carbon cycle that vary at different time scales, yet to quantify such impacts with a simple scheme is traditionally deemed difficult. Here, we show that, by incorporating a tem- perature sensitivity parameter （1.64 ppm yr-1 ℃-1） into a simple linear carbon-cycle model, we can accurately characterize the dynamic responses of atmospheric carbon dioxide （CO2） concentration to anthropogenic carbon emissions and global temperature changes between 1850 and 2010 （r2 〉 0.96 and the root-mean-square error 〈 1 ppm for the period from 1960 onward）. Analytical analysis also indicates that the multiplication of the parameter with the response time of the atmospheric carbon reservoir （-12 year） approximates the long-term temperature sensitivity of global atmospheric CO2 concentration （-15 ppm ℃ 1）, generally consistent with previous estimates based on reconstructed CO2 and climate records over the Little Ice Age. Our results suggest that recent increases in global surface temperatures, which accelerate the release of carbon from the surface reservoirs into the atmosphere, have partially offset surface carbon uptakes enhanced by the elevated atmo- spheric CO2 concentration and slowed the net rate of atmospheric CO2 sequestration by global land and oceans by -30% since the 1960s. The linear modeling framework outlined in this paper thus provides a useful tool to diagnose the observed atmospheric CO2 dynamics and monitor their future changes.

关键词： atmospheric CO2 dynamics climate-carbon interactions climate change carbon cycle

Recent Advances in Metal–Organic Frameworks Based on Electrospinning for Energy storage

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Advanced Fiber Materials 2023年第5期5卷 1592-1617页

作者： shunyu Yao seeram ramakrishna Gang Chen MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001People’s Republic of China Center for Nanotechnology and sustainability Department of Mechanical EngineeringNational University of SingaporeSingapore 119260Singapore

Metal–organic frameworks are linked by different central organic ligands and metal-ion coordination bonds to form periodic pore structures and rich pore *** of their structural advantages,metal–organic frameworks are considered to be one of the most promising candidates for new energy storage *** better utilize their advantages,metal–organic frameworks can be combined with electrospinning technology to effectively adjust the porosity and mechanical strength of composite *** paper summarizes the combination of the latest metal–organic frameworks and classical spinning technology,starting from the structural design of electrode materials,and applying them to supercapacitors,lithium-ion batteries,lithium–sulfur batteries,sodium-ion batteries,and potassium-ion ***,the problems and challenges related to the preparation of metal–organic framework nanofibers are summarized,and future development trends are predicted.

关键词： Metal-organic frameworks(MOFs) supercapacitors Electrospinning Lithium-ion batteries sodium-ion batteries

A facile and convenient synthesis of novel imidazo[1,2-b] isoxazoles and their Mannich bases as potential biodynamic agents

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Chinese Chemical Letters 2015年第12期26卷 1511-1513页

作者： E.Rajanarendar K.Thirupathaiah s.ramakrishna D.Nagaraju Department of Chemistry Kakatiya University

A series of novel imidazo[1,2-b]isoxazoles 3 and their Mannich bases 4–6 were synthesized via convenient reactions. The reaction of 3-aminoisoxazole 1 with substituted phenacyl bromides 2 in dry ethanol afforded the corresponding 6-methyl-3-aryl imidazo[1,2-b]isoxazoles 3 in good *** 3 on treatment with 37% formaline and secondary amines furnished the corresponding novel Mannich bases viz., 6-methyl-3-aryl-2-（morpholine/pyrrolidin-1-yl/piperidin-1-yl）-methyl-imidazo[1,2-b]isoxazoles 4–6.

关键词： convenient facile bases substituted amines antibacterial acetic chloroform ethanol pyrrolidine