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Chinese Journal of Natural Medicines 2024年第5期22卷 466-480页

作者： LIANG Haizhen CHEN Xiaojuan LI Qi ZHANG Mengmeng LU Pengxin ZHANG Jie SONG Juan ZHANG Tao GUO Baolin ma baiping Department of Pharmaceutical Sciences Beijing Institute of Radiation MedicineBeijing 100850China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100193China AnKang Chia Tai Pharmaceutical Co. Ltd.Shaanxi 725000China

Sixteen new dammarane-type triterpenoid saponins(1-16)featuring diverse structural variations in the side chain at C-17,along with twenty-one known analogues(17-37),have been isolated from the rhizomes of Gynostemma longipes ***,a plant renowned for its medicinal and edible *** structural elucidation of these compounds was accomplished through comprehensive analyses of 1D and 2D NMR and HRMS spectroscopic data,supplemented by comparison with previously reported *** assays on the isolates for their protective effects against hypoxia-induced damage in pheochromocytoma cells(PC12 cells)revealed that nine saponins exhibited significant anti-hypoxic *** investigation into the anti-hypoxia mechanisms of the representative saponins demonstrated that compounds 22 and 36 markedly reduced the levels of hypoxia-induced ***,these compounds were found to decrease the release of lactate dehydrogenase(LDH)and malondialdehyde(MDA),while increasing the activity of superoxide dismutase(SOD),thereby indicating that the saponins could mitigate hypoxia-induced injuries by ameliorating apoptosis and oxidative *** findings offer substantial evidence for the future utilization and development of ***,identifying dammarane-type triterpenoid saponins as its active anti-hypoxic constituents.

关键词： Gynostemma longipes Dammarane-type saponins Anti-hypoxia effect Apoptosis

Insights into dammarane-type triterpenoid saponin biosynthesis from the telomere-totelomere genome of Gynostemma pentaphyllum

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Plant Communications 2024年第8期5卷 100-103页

作者： Lingling Yun Chuyi Zhang Tongtong Liang Yu Tian Guoxu ma Vincent Courdavault Sijie Sun baiping ma Ziqin Li Rucan Li Feng Cao Xiaofeng Shen Jianhe Wei Ying Li Baolin Guo Chao Sun Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100193China Biomole cules et Biotechnologies Ve ge tales EA2106Universite de Tours37200 ToursFrance Beijing Institute of Radiation Medicine Beijing 100850China Present address:National Genomics Data Center Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China

Dear Editor,Gynostemma pentaphyllum(Thunb.)makino,a perennial climbing vine in the Cucurbitaceae family,has been widely used in traditional medicine for over 600 years(Blumert and Liu,1999).It serves as a valuable natural source of over 200 dammaranetype saponins with notable bioactive properties,including anti-cancer,cardioprotective,hepatoprotective,neuroprotective,and anti-diabetic activities(Li et al.,2016;Nguyen et al.,2021).

关键词： protective Gynostemma saponins

酸枣仁种皮和种仁化学成分的定性及半定量分析

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色谱 2024年第3期42卷 234-244页

作者：石宇浩南易郑伟姚兰梁海珍陈晓娟宋娟张洁贾德贤王谦马百平天津中医药大学天津301617 军事科学院军事医学研究院辐射医学研究所北京100850 北京中医药大学北京100029

酸枣仁为鼠李科植物酸枣Ziziphus jujuba ***(Bunge)Hu ex ***的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据。基于超高效液相色谱-四极杆飞行时... 详细信息

酸枣仁为鼠李科植物酸枣Ziziphus jujuba ***(Bunge)Hu ex ***的干燥成熟种子,其分为种皮、种仁两个部位,比较研究酸枣仁不同部位化学成分组成及相对含量可为合理开发利用中药酸枣仁资源提供理论依据。基于超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)技术,从种皮、种仁中共鉴定出57个化学成分。结合主成分分析(PCA)和正交偏最小二乘法判别分析(OPLS-DA)对两者进行差异成分研究,以变量投影重要度(VIP)值>5为标准,筛选了差异成分17个,其中白桦脂酸、桦木酮酸、麦珠子酸和酸枣仁皂苷Ⅰ主要存在于种皮部位,斯皮诺素、酸枣仁皂苷A和6-阿魏酰斯皮诺素等13个化合物主要存在于种仁部位。通过超高效液相色谱-电雾式检测器(UPLC-CAD)结合反梯度补偿技术,建立半定量液相色谱指纹图谱,考察了6个不同结构类型的代表成分的响应一致性,其不同浓度下平均响应因子间的RSD值为7.04%,各化合物响应一致性良好,可用于酸枣仁的半定量表征分析;结果表明:种皮部位主要成分为白桦脂酸和油酸,其中白桦脂酸的含量约是种仁的7倍;种仁部位主要成分为斯皮诺素、酸枣仁皂苷A、亚油酸、白桦脂酸和油酸,其中斯皮诺素、酸枣仁皂苷A的含量分别是种皮的18倍和24倍。综上,本研究阐明了酸枣仁种皮、种仁的化学成分差异,明确了酸枣仁两个部位中各自的主要成分及其相对含量,为酸枣仁不同部位合理开发和利用奠定了基础。

关键词：超高效液相色谱电雾式检测器质谱差异成分酸枣仁种皮种仁半定量分析

Lycium barbarum polysaccharide ameliorates radiation-induced brain injury by regulating gut microbiota

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Journal of Traditional Chinese Medical Sciences 2023年第1期10卷 42-51页

作者： Jinglu Hu Chunqing Wang Qi Li Wencheng Jiao Xiaojuan Chen baiping ma Lina Du Pharmaceutical College of Henan University Henan UniversityKaifeng 475004China Department of Pharmaceutical Sciences Beijing Institute of Radiation MedicineBeijing 100850China School of Pharmacy Shandong University of Traditional Chinese MedicineJinan 250355China Institute of Life Sciences and Green Development Hebei UniversityBaoding 071002China

Objective: To determine the extent to which Lycium barbarum polysaccharide(LBP) improves60Co γ-ray radiation-induced brain injury(RIBI) by regulating the gut ***: The RIBI model of mice was established with the appropriate dose of60Co γ-ray to identify the changes in the body weight, behaviors, gut microbiota, and inflammatory reactions of mice. Mice were randomly divided into healthy, RIBI model, and LBP groups. The related inflammatory cytokines were determined using an enzyme linked immunosorbent assay kit. Then, 16S rRNA sequencings of feces were carried out to evaluate the differences in intestinal ***: Compared with the spontaneous activity and exploratory spirit of the healthy group, those traits in the RIBI model mice in the open field significantly decreased, the freezing time in the elevated plus maze(EPM) significantly increased, and the number of times the mice discriminated the novel object was significantly lower. Hematoxylin-eosin slides showed that the main histopathological changes of RIBI occurred in the hippocampus. In addition, the diversity and relative abundances ratio of the gut bacterial phylum, order, family, and genus in the model group varied widely. Changes in Bacteroidetes,Firmicutes, and Proteobacteria were the most obvious after head radiation exposure. In comparison, LBP could accelerate the recovery of weight loss in RIBI mice. The frequency that mice entered the center of the open field, facing the open arm in the EPM, and the number of times they discriminated the novel object were significantly increased with LBP administration. LBP could also reduce the levels of inflammatory factor caused by RIBI. LBP increased the diversity and abundance of gut microbiota in RIBI model mice. In addition, LBP increased the relative abundance of Bacteroidetes but decreased the levels of Firmicutes and Proteobacteria for irradiated ***: LBP can improve depression and tension by regulating the composition of gut

关键词： Lycium barbarum polysaccharide Gut microbiota Diversity Radiation Brain injury Behavior Cognition Inflammatory

Preventive effect of nasal Timosaponin BII-loaded temperature-/ionsensitive in situ hydrogels on Alzheimer’s disease

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Journal of Traditional Chinese Medical Sciences 2021年第1期8卷 59-64页

作者： Ge Ou Wenyuan Chen Meiyan Yang Qian Li Yuanyuan Zhang Delian Wan Lina Du baiping ma Department of Pharmaceutical Sciences Beijing Institute of Radiation MedicineBeijing 100850China Pharmacy Department Chinese PLA General HospitalBeijing 100853China School of Pharmaceutical Sciences Shandong University of Traditional Chinese MedicineJinan 250355China Department of Pharmaceutics Beijing Institute of Pharmacology and ToxicologyBeijing 100850China

Objective:To study the preventive effect of Timosaponin BII(T-BII)-loaded temperature/ion-sensitive nasal in situ hydrogels(ISGs)on Alzheimer's disease(AD),its preparation technology,characteristics and in vivo effects were ***:The morphological and rheological properties were *** preventive effects of T-BII ISG on scopolamine-induced AD in mice were determined with the index of muscarinicreceptor 1(M1)expression and pathological ***:Results revealed that T-BII ISG significantly increased the content of M1 choline receptors in the hippocampus of mice and ameliorated the damage incurred to the hippocampal cornu ammonis 1(CA1)***:T-BII ISGs is a reasonable and convenient method of exerting an obvious preventive effect on mice with AD induced by ***,thereby,lays forth a new treatment option for preventing AD.

关键词： Timosaponin BII Alzheimer’s disease In situ hydrogels Intranasal administration

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运输类飞机的机身抗烧穿性适航要求解析

航空工程进展 2017年第3期8卷 308-314页

作者：马百平李翰邹田春解江中国民航大学天津市民用航空器适航与维修重点实验室天津300300

运输类飞机可生存坠撞后的火灾是客舱安全范畴内的重要问题之一。提高机身抗烧穿性,即推迟火焰进入客舱的时间,可为乘员应急撤离提供时间保证。介绍了运输类飞机坠撞后的池火火焰特征,包括最高温度、热流密度等特征的范围及其影响因素,... 详细信息

运输类飞机可生存坠撞后的火灾是客舱安全范畴内的重要问题之一。提高机身抗烧穿性,即推迟火焰进入客舱的时间,可为乘员应急撤离提供时间保证。介绍了运输类飞机坠撞后的池火火焰特征,包括最高温度、热流密度等特征的范围及其影响因素,重点论述了机身结构烧穿过程,剖析了适航规章中的抗烧穿符合性判据,梳理了抗烧穿性试验方法的发展过程,最后对我国未来运输类飞机机身抗烧穿研究的发展进行了展望。

关键词：抗烧穿运输类飞机客舱安全适航池火

指纹图谱技术考察桔梗在天王补心丸中的配伍意义

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中国中药杂志 2009年第2期34卷 156-160页

作者：刘萍邹鹏李外孙艳张洁余河水赵阳马百平解放军总医院中药房北京100853 军事医学科学院放射与辐射医学研究所北京100850

目的:建立天王补心丸活性部位的高效液相(HPLC)指纹图谱,从化学成分角度阐述桔梗舟楫作用的物质基础和科学内涵。方法:采用Hanbon Lichrospher C_(18)色谱柱(4.6mm×250 mm,5μm),流动相甲醇和0.012 5 mol·L^(-1)乙酸铵-醋酸水溶液线... 详细信息

目的:建立天王补心丸活性部位的高效液相(HPLC)指纹图谱,从化学成分角度阐述桔梗舟楫作用的物质基础和科学内涵。方法:采用Hanbon Lichrospher C_(18)色谱柱(4.6mm×250 mm,5μm),流动相甲醇和0.012 5 mol·L^(-1)乙酸铵-醋酸水溶液线性梯度洗脱,流速为1.0 mL·min^(-1),检测器为二极管阵列检测器(PDA)与蒸发光散射检测器(ELSD)串联使用,建立指纹图谱;将全方组与缺桔梗组、桔梗组与单独桔梗组及单独酸枣仁组进行指纹图谱比较;同时比较各组样品提取的样品量。结果:在提取方法相同且药材量相当的条件下,天王补心丸全方组提取得到的样品量明显高于缺桔梗组;桔梗+酸枣仁组提取得到的样品量明显高于桔梗组、酸枣仁组单煎得到的样品量之和;得到各组分离度较好的HPLC-PDA(max Plot)和HPLC-ELSD 2种指纹图谱,且2者具有良好的相关性和互补性。结论:桔梗可以增加天王补心丸及主要药味酸枣仁水煎提取得到的样品量,可以增加其中某些成分的溶出及含量。

关键词：天王补心丸桔梗舟楫作用高效液相色谱法指纹图谱

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人参皂苷Rh_2及其衍生物的研究进展

世界科学技术-中医药现代化 2012年第6期14卷 2205-2211页

作者：曹满张洁赵阳宋新波马百平军事医学科学院放射与辐射医学研究所北京100850 天津中医药大学研究生院天津300193 天津中一制药有限公司天津300193

人参皂苷Rh2是红参中特征性成分之一,也是一种生物活性较高的稀有皂苷。近年来,人参皂苷Rh2的制备研究由酸水解法、碱水解法、合成法逐渐转到生物转化法,微生物及酶转化法以其独特优势成为制备人参皂苷Rh2的理想方法;人参皂苷Rh2及其衍... 详细信息

人参皂苷Rh2是红参中特征性成分之一,也是一种生物活性较高的稀有皂苷。近年来,人参皂苷Rh2的制备研究由酸水解法、碱水解法、合成法逐渐转到生物转化法,微生物及酶转化法以其独特优势成为制备人参皂苷Rh2的理想方法;人参皂苷Rh2及其衍生物的生物活性、药代动力学等也均有较多研究。本文对人参皂苷Rh2及其衍生物的制备、药理活性等方面进行综述,为该化合物深入研究开发提供参考。

关键词：人参皂苷Rh2 制备药理活性药代动力学

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中药黄精炮制的历史沿革及现代研究

河南中医学院学报 2005年第3期20卷 28-29,31页

作者：张洁杨云马百平河南中医学院军事医学科学院放射医学研究所

对黄精炮制的历史沿革及现代研究进行分析,指出黄精炮制研究中存在的问题及其解决方法,为黄精炮制的系统研究奠定了基础。

关键词：黄精炮制历史沿革现代研究

定量蛋白质组学研究揭示知母可缓解2型糖尿病模型大鼠肝脏代谢异常

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生物工程学报 2022年第10期38卷 3888-3900页

作者：门丽影张涛武舒佳马百平董悦生常蕾张瑶徐平安徽医科大学基础医学院安徽合肥230032 军事科学院军事医学研究院生命组学研究所中国医学科学院蛋白质组学与药物研发新技术创新单元国家蛋白质科学中心(北京)北京蛋白质组研究中心蛋白质组学国家重点实验室北京102206 军事科学院军事医学研究院辐射医学研究所北京100850 大连理工大学生物工程学院辽宁大连116024

2型糖尿病(type 2 diabetes mellitus,T2DM)是一种在全球范围内广泛存在的代谢性疾病,不及时治疗可能会引发众多危及生命的并发症。肝脏代谢在糖尿病发生发展的过程中扮演着至关重要的角色。目前已有报道中药知母用于缓解胰岛素抵抗及... 详细信息

2型糖尿病(type 2 diabetes mellitus,T2DM)是一种在全球范围内广泛存在的代谢性疾病,不及时治疗可能会引发众多危及生命的并发症。肝脏代谢在糖尿病发生发展的过程中扮演着至关重要的角色。目前已有报道中药知母用于缓解胰岛素抵抗及糖尿病,但其能否缓解糖尿病中肝脏代谢的异常仍有待深入研究。因此,提取了高脂饮食和化学药物链脲佐菌素(streptozotocin,STZ)诱导的2型糖尿病大鼠模型、知母提取物处理的2型糖尿病大鼠模型、高脂饮食大鼠模型以及正常饮食大鼠对照组的肝脏蛋白,通过基于质谱的定量蛋白质组学串联质量标签(tandem mass tag,TMT)标记技术获得定量蛋白质组数据。利用生物信息学软件对各组数据进行层次聚类分析及主成分分析,并以P1.5作为阈值标准进行火山图分析,发现知母提取物治疗组相较未治疗组与正常对照组更接近,表明肝脏组织定量蛋白质组数据能够反映知母提取物对2型糖尿病大鼠模型的治疗效果。筛选获得了表达水平与知母提取物治疗密切相关的关键蛋白簇。利用在线网站分析蛋白簇的GO功能与KEGG通路,发现知母缓解2型糖尿病模型大鼠肝脏脂肪酸代谢异常可能与关键蛋白Ndufa6和Prkar2b的表达水平回调有关。

关键词： 2型糖尿病肝脏知母定量蛋白质组学脂肪酸代谢