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Reference ranges and age-related changes of peripheral blood lymphocyte subsets in Chinese healthy adults

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Science China(Life sciences) 2009年第7期52卷 643-650页

作者： JIAO Yang1,QIU ZhiFeng1,XIE Jing1,LI DongJing2 & LI TaiSheng1 1 Department of Infectious Disease,Peking Union medical college Hospital,Peking Union medical college & Chinese Academy of medical sciences,Beijing,China 2 Department of Special Care Unit,Peking Union medical college Hospital,Peking Union medical college & Chinese Academy of medical sciences,Beijing,China Department of Infectious Disease Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China Department of Special Care Unit Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing China

This study was performed to build region-specific reference ranges of peripheral blood lymphocyte subsets for Chinese healthy adults from the young to the elderly and analyze the trends of changes in lymphocyte subsets for evaluating the impact of age on the values.151 healthy adults aged 19-86 were recruited based on the SENIEUR *** sets of reference ranges were finally built applicable for the healthy young(19-44 years),middle-aged(45-64 years) and elder adults(≥65).Comparisons in parameters among the three cohorts showed that a statistically significant increase in CD16CD56+ NK cell was observed between the middle-aged and elder cohorts,whereas for the majority of the parameters,a significant decline was observed between the young and the middle-aged *** results showed that inverse correlations were observed between the age and CD19+ B,CD3+ T,CD3+CD4+ T,CD4+CD45RA+CD62L+ nave T cell and CD4+CD28+/CD4+,while the positive one was identified between the age and the NK *** significant changes of the most of immune parameters provided evidence for ***,T cell activation markers of CD8+CD38+ and CD8+HLA-DR+ showed reverse trends of association with age,which provides a clue for further researches on the mechanisms underlying the paradoxical clinical presentation of the elder patients.

关键词： lymphocyte subsets age reference range immunosenescence

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Chromatin domain boundaries: insulators and beyond

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Cell Research 2005年第4期15卷 292-300页

作者： Chih Chuan LIANG National Laboratory of medical Molecular Biology Institute of basic medical sciences Chinese Academy of medicalsciences and Peking Union medical college 5 Dong Dan San Tiao Beijing 100005 China

The eukaryotic genome is organized into functionally and structurally distinct domains, representing regulatory unitsfor gene expression and chromosome behavior. DNA sequences that mark the border between adjacent domains are theinsulators or boundary elements, which are required in maintenance of the function of different domains. Some insula-tors need others enable to play insulation activity. Chromatin domains are defined by distinct sets of post-translationallymodified histones. Recent studies show that these histone modifications are also involved in establishment of sharpchromatin boundaries in order to prevent the spreading of distinct domains. Additionally, in some loci, the high-orderchromatin structures for long-range looping interactions also have boundary activities, suggesting a correlation betweeninsulators and chromatin loop domains. In this review, we will discuss recent progress in the field of chromatin domainboundaries.

关键词： insulator chromatin domain boundary histone code chromatin loop domain.

Inhibition of the α-mannosidase Man2c1 gene expression enhances adhesion of Jurkat cells

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Cell Research 2006年第7期16卷 622-631页

作者： Li Qu~(1,*) Ji Yu Ju~(1,*) Shuang Ling Chen~1 Yan Shi~1 Zhi Guang Xiang~1 Yi Qun Zhou~1 Yun Tian~1 Yin Liu~1 Li Ping Zhu~1 ~1Department of Immunology,Institute of basic medical sciences,Chinese Academy of medical sciences and Peking Union medical college,Beijing 100005,China Department of Immunology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

Protein N-glycosylation plays very important roles in immunity and α-mannosidase is one of the key enzymes in *** paper reports that inhibition of α-mannosidase Man2cl gene expression enhances adhesion of JurkatT *** comparison to the controls with normal expression of the enzyme,Jurkat cells with the inhibition of Man2clgene expression（AS cell）formed larger aggregates in culture,indicating an enhancement of adhesion between the *** differential display analysis discovered up-regulation of several adhesion molecule genes in the AS *** the pivotal role played by CD54-LFA-1 interaction in immune cell interaction,this study focused on the contributionof enhanced expression of CD54 and LFA-1 to the enhanced adhesion of AS Jurkat *** facts,including increasedbinding of AS cells to ICAM-1-Fc,Mg2+activation of the binding of AS cells to ICAM-1-Fc and enhanced aggregationof AS cells,together with the inhibiting effect of a blocking CD 11a mAb on the binding to ICAM-1-Fc and aggregationof the cells demonstrate an important contribution of enhanced CD54-LFA-1 interaction to increased adhesion betweenAS *** enhanced CD54-LFA-1 interaction also resulted in increased adhesion between AS Jurkat T cells and RajiB *** addition,AS cells showed cytoskeletal *** data imply a biological significance of MAN2C1in T-cell functioning.

关键词： Man2cl Jurkat T cell adhesion CD54-LFA-1 cytoskeleton

MUTUAL POTENTIATION OF THE ANA-LGSIC EFFECT OF MORPHINE MET-ENKE-PHALIN AND DYNORPHIN AND THEIR ANTAGONISM BY NALOXONE

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针刺研究 1989年第Z1期 150-151页

作者： Wang Junfeng Ren Minfeng Han Jisheng Department of physiology Beijing Nedical University Institute of Acupuncture Science, An Hui college of Traditional Chinese medical Institute of basic medical sciences, Chinese Academy of medical Science

The present study deals primarily with the antagonistic effect of Naloxone(NX) against mutual potentiation of morphine (Mor), mek-enkephalin (MEK)and dyno phin (Dyn) in their analgesic effects. (1) The rats were divided into 4 groups, ie. NS, NX 0. 02, 0. 1 and 0. 5mg/kggroups. A mixture of Dyn 2. 5noml and Mot 7. 5noml was injected in thebeginning via intrathecal cannulae into each rat. Percentage incrcase in TFLin all 4 groups was between 94±19% and 109±20% 10rain later. After thateach grcup was injected subcutaneously NS, NX 0.02, 0.1 and 0.5mg/kg respe-ctively and was observed continuously for 50min. The results suggest that the

关键词： NX NS MUTUAL POTENTIATION OF THE ANA-LGSIC EFFECT OF MORPHINE MET-ENKE-PHALIN AND DYNORPHIN AND THEIR ANTAGONISM BY NALOXONE ANA TFL

High-throughput screening for monoamine oxidase-A and monoamine oxidase-B inhibitors using one-step fluorescence assay

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Acta Pharmacologica Sinica 2006年第6期27卷 760-766页

作者： Hong-mei GUANG Guan-hua DU~2 Institute of Materia Medica,Peking Union medical college and Chinese Academy of medical sciences,Beijing 100050,China Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Beijing China

Aim:To develop high-throughput screening(HTS)assays for monoamine oxi-dase(MAO)-A and MAO-B ***:A fluorescence probe basedmethod measuring MAO-A and MAO-B activity was established and optimized,with its sensitivity,stability and specificity *** conditions in-cluding enzyme sources,substrate concentrations,incubation volume and reac-tion time in 384-well format were optimized to achieve sensitive and low consump-tive ***:In optimized conditions,dynamic parameters of MAO-A andMAO-B were *** Kmvalue of serotonin to MAO-A was 1.66μmol/L,while that of benzylamine to MAO-B was 0.80 μmol/*** IC50value of clorgylineto MAO-A was 2.99 nmol/L,and that of deprenyl to MAO-B was 7.04 nmol/L,matching those obtained from traditional spectrometric *** testedsamples,one compound exerted an inhibitory effect on MAO-A activity with IC50as 0.36 μmol/L,and three compounds had an inhibitory effect on MAO-B activitywith IC50as 0.13,0.19,and 0.13 μmol/*** Z′ factor was 0.71±0.03 and 0.75±0.03in MAO-A-inhibitor and MAO-B-inhibitor HTS system,***:The established assays can be well applied to MAO-A and MAO-B inhibitorscreening with high quality,precision and reproducibility.

关键词： fluorescence assay monoamine oxidase preclinical drug evaluation

MEASUREMENTS OF INTRAOCULAR PRESSURE THROUGH0UT THE PREGNANCY IN PAKISTANI WOMEN

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Chinese medical sciences Journal 1997年第1期12卷 53-56页

作者： Imran Ahmad Qureshi(Department of physiology, Rawalpindi medical college, Rawalpindi, Pakistan)(foreign student dorm, Shanghai medical University, 200032) Department of physiology Rawalpindi Medical College Rawalpindi Pakistan. Physiology Department Basic Medical Sciences Institute Jinnah Postgraduate Medical Centre Karachi Pakistan.

Background. Decrease in intraocular pressure (IOP) during pregnancy has been reported by previous studies,but amount of decrease varies from study to study. Moreover,previous studies have concentrated on the last trimester of pregnancy or one reading per trimester. IOP changes throughout the pregnancy have never been described. Therefore,the present study was planned to determine IOP throughout the pregnan cy,after placing control on IOP affecting *** and methods. Thirty-six pregnant and same numbers of non-pregnanat women of the same age group were studied. IOP measurements were taken at four week intervals throughout the pregnancy,with the Goldmann applanation ***. As compared to control subjects,up to 8th week,IOP remained the same. At 12th week,it becomes significantly lower. The IOP differences between fIrst and second (P<0. 05),and second and third (P<0. 001 ) trimesters of pregnancy were significant. In every subject, IOP decreased during ***,there were variations among individuals in the extent of IOP decrease Conclusbos. With advancing pregnancy, intraocular pressure decreases. Knowledge of the normal level of intraocular pressure in various stages of pregnancy may help glaucoma screeners.

关键词： ocular pressure pregnancy tonometry

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Protective effects of compound FLZ onβ-amyloid peptide-(25-35)-induced mouse hippocampal injury and learning and memory impairment

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Acta Pharmacologica Sinica 2006年第6期27卷 651-658页

作者： Fang FANG Geng-tao LIU~2 Department of Pharmacology,Institute of Materia Medica,Peking Union medical college and Chinese Academy of medical sciences,Beijing 100050.China Department of Pharmacology Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Beijing China

Aim:To study the protective effects of compound FLZ,a novel synthetic ana-logue of natural squamosamide,on learning and memory impairment and lesionsof the hippocampus caused by icv injection of β-amyloid25-35(Aβ25-35)in ***:Mice were icv injected with the Aβ25-35(15 nmol/mouse),and thentreated with oral administration of 75 mg/kg or 150 mg/kg of FLZ once daily for 16consecutive *** impairment of learning and memory in mice were testedusing step-down test and Morris water maze *** content of malondialdehyde(MDA)and the expressions of acetylcholinesterase(ACHE),Bax,and Bcl-2 in theCA1 region of the mouse hippocampus were measured by biochemical and immu-nohistochemical analysis,*** pathological damages of hippocam-pus were observed using a ***:FLZ(75 mg/kg,150 mg/kg)significantly attenuated Aβ25-35-induced impairment of learning and memory in thestep-down test and Morris water maze *** also reduced pathological dam-ages to the hippocampus induced by Aβ25-35Furthermore,FLZ prevented theincrease of AChE and Bax,and the decrease of Bcl-2 immunoreactive cells in theCA1 region of the hippocampus,and reduced the increase of MDA content in thehippocampus in mice injected with Aβ***:FLZ has protective ac-tion against the impairment of learning and memory and pathological damage tothe hippocampus induced by icv injection of Aβ25-35in mice.

关键词： FLZ Aβ25-35 intracerebroventricular injection hippocampus acetylcholinesterase Bax Bcl-2 malondialdehyde

Resveratrol attenuates brain damage in a rat model of focal cerebral ischemia via up-regulation of hippocampal Bcl-2

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Resveratrol attenuates brain damage in a rat model of focal ...

Function of chymase in the heart angiotensin Ⅱ formation in transgenic mice

第九届全国钙信号和细胞功能研讨会

作者： Zhen Li,Liecheng Wang Department of physiology.college of basic medical sciences,Anhui medical University,Hefei, Anhui 230032,China

＜正＞A number of studies have demonstrated that resveratrol（Res）,a natural polyphenol compound found in plants,shows potent neuroprotective,anti-inflammatory and antioxidant effects;however,its ability to prevent ischemia-induced brain damage remains *** we tested whether Res played a neuroprotective role in a rat brain ischemia model induced by middle cerebral arteryocclusion（MCAO）.Adult male rats were randomly assigned into four experimental groups:sham operation （sham）,ischemia treatment（MCAO）,Res-treated MCAO（Res+MCAO）and Res

关键词： Resveratrol Middle cerebral artery occlusion Neuroprotection

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Chinese Science Bulletin 2001年第11期46卷 922-925页

作者： LI Peng & CHEN LanyingCardiovascular Institute & Fu Wai Hospital, Chinese Academy of medical sciences & Peking Union medical college, Beijing 100037, China Cardiovascular Institute & Fu Wai Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing China

The myosin light chain 2 promoter-human heart chymase (MLC2-hChymase) transgenic mice founded by our laboratory were used as the model to study the function of chymase in the heart angiotension Ⅱ (Ang Ⅱ) formation and heart remodeling. Tissue-specific expression of human heart chymase gene and transcriptional expression of type Ⅰ and type Ⅲ collagens genes were analyzed by RT-PCR. Activities of chymase, ACE and the levels of Ang Ⅱ in heart and plasma were determined with radioimmunoas-say (RIA) kit. Activity of heart matrix metalloproteinase-9 (MMP-9) was detected using gelatin zymography. The cardiac hypertrophic phenotypes were also observed with the physiological and morphological methods. The results in the MLC2-hChymase transgenic mice indicated: ( i ) human heart chymase gene was expressed specially in the heart; (u) heart chymase activity increased markedly in the transgenic mice vs non-transgenic mice (control) (0.27±0.07 U/mg vs. 0.15±0.02 U/mg, P<0.05) with no significant difference in

关键词： chymase transgenic mice angiotensin Ⅱ heart remod- eling collagen.

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