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Journal of Biomaterials and Nanobiotechnology 2013年第1期4卷 98-106页

作者： Alicia Valeria Zaranza Francyelle Costa Morais Monique Santos do Carmo Adriana de Mendonca Marques Cristina Andrade-Monteiro Thiago Feitosa Ferro Valerio Monteiro-neto Patricia de Maria silva Figueiredo Research Center in Parasitic Diseases and Endemic University of CEUMAUniCEUMASao LuisBrazil

A hundred Pseudomonas aeruginosa strains from several clinical specimens from five hospitals in Sao Luís-MA were evaluated for biofilm production, prevalence of the gene algD, adhesion to HEp-2 cells and antimicrobial susceptibility. The most affected clinical specimens and hospital sectors were also evaluated. Most isolates were obtained from the tracheal aspirate (21.0%) and the most affected hospital sector was the ICU (43.0%). The antibiotics with the highest sensitivity rate were amikacin, piperacillin/tazobactam, fluoroquinolones, gentamicin and meropenem and the ones with the highest resistance rate were aztreonam, ceftazidime and cefepime. All samples were sensitive to polymyxin B. In relation to the expression of the gene for ESBL, 50.0% (17/34) of the multiresistant strains showed the enzyme TEM. Most strains showed high hydrophobicity and 96% of the isolates produced biofilm on a polystyrene microplate, 52% were capsule producers, 19% showed mannose-sensitive fimbriae and 39% expressed the gene algD. We observed adhesion to HEp-2 cells and to the coverslip. These factors may be reported in the pathogenesis of this bacterium, what represents a potential risk for colonization of medical devices which favor the establishment of chronic nosocomial infections.

关键词： Pseudomonas aeruginosa Antimicrobial Biofilm Adhesion

Use of radiation in strains of Saccharomyces cerevisiae:A new technique for industrial applications

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Advances in Bioscience and Biotechnology 2013年第3期4卷 346-351页

作者： Rafael O.Andrette Joao D.T.Arruda-neto Thiago O.Basso Luiz Carlos Basso Erika Cavalcante-silva Maria C.Bittencourt-Oliveira Godofredo C.Genofre Physics Institute University of Sao PauloSao PauloBrazil CEPESq/UniItalo-Italy-Brazilian University Center Sao PauloBrazil Inter-Unities Graduation Program on Biotechnology University of Sao PauloSao PauloBrazil Department of Biological Sciences Luiz de Queiroz College of Agriculture/ESALQUniversity of Sao PauloPiracicabaBrazil Laboratory of Cyanobacteria ESALQUniversity of Sao PauloSao PauloBrazil Institute of Biosciences University of Sao PauloSao PauloBrazil

During the industrial fermentation process in the production of fuel ethanol, yeasts are subject to several stressing conditions. The survival and the permanence of strains introduced in the process correlate with the capability of these yeasts in resisting to physical and chemical stresses, as well as their recovering ability to compete with contaminating micro-organisms commonly present in this industrial process. We aim at the selection of Saccharomyces cere visiae strains having this capability and ability. In this sense, cultivations of strains with industrial interest were irradiated with gammas ray at a wide dose interval. Growing curves for the strains were analyzed by means of their relative growth, a new concept here introduced, which allows a better understanding of the growing and recovering processes following radiative stress. It was found that gamma radiation could be used as an alternative method to quantify growing capabilities of S. cerevisiae strains under stressing conditions. It was also shown that this radiological method could be utilized as an additional procedure to select best robust industrial strains. This radiological method simplifies traditional analysis of strain viability, by avoiding the great number of necessary and consecutive fermentation assays.

关键词： Saccharomyces cerevisiae Industrial Yeast Gamma Radiation Evaluation Method Industrial Fermentation Ethanol

Clinical Outcomes of Positive Newborns for COVID-19 in Rio de Janeiro, Brazil

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Open Journal of Obstetrics and Gynecology 2021年第12期11卷 1851-1861页

作者： Karina Martins-Cardoso Luana Ferreira Martins de Toledo Juliana Aparecida Souza Paz Andressa Rangel de Oliveira Lima Thaís Chrispim de Souza Giangiarulo Jéssica da silva Oliveira IPB COVID Workgroup Carla Carolina Teixeira Jones Zitenfeld Cardia neto Giovanna Geórgia Pires Carrilho Vallim Shana Priscila Coutinho Barroso Laboratório de Biologia Molecular Instituto de Pesquisas Biomédicas Hospital Naval Marcílio Dias Rio de Janeiro Brasil Instituto de Bioquímica Médica Leopoldo de Meis Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil UTI neonatal Clínica de Pediatria Hospital Naval Marcílio Dias Rio de Janeiro Brasil Serviç o de Patologia Hospital Naval Marcílio Dias Rio de Janeiro Brasil

The disease caused by the SARS-CoV-2 virus has spread very rapidly throughout the world, causing various clinical manifestations and affecting several organs and systems. However, the possibility of vertical transmission of COVID-19 in symptomatic pregnant women has been questioned. The aim of this work was to report cases of possible vertical transmission in pregnant women affected by the SARS-CoV-2 virus, as well as its clinical repercussions on the newborn. In this paper, we conducted a cross-sectional retrospective study by analyzing medical records of pregnant women admitted at the military hospital, in Rio de Janeiro, Brazil, diagnosed with COVID-19 and who had positive newborns. To evaluate epidemiological data, clinical manifestations, laboratorial and histopathological changes were related to such vertical transmission. Only six tested newborns were positive for COVID-19. The mothers had no respiratory symptoms, and the gestational age was at term, except for one newborn born prematurely due to detection of pericadial effusion. Three neonates had respiratory symptoms, requiring ventilatory support. None of the mothers or newborns died. During the pandemic, possible vertical transmission cases of infected pregnant women in the third trimester of pregnancy were observed. According to the case reports published in the literature, they illustrate phenotypes of neonatal involvement with varied clinical manifestations and severity;however, further scientific studies are needed to prove the effective vertical transmission of SARS-CoV-2 in positive pregnant women.

关键词： SARS-CoV-2 Infection Neonatal Outcomes Vertical Transmission

Economic Feasibility Evaluation of the Use of Wind Microgenerator in Multifamily Building in Southern Brazil

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Journal of Civil Engineering and Architecture 2022年第10期16卷 513-518页

作者： Lauro AndréRibeiro Marcos Vinícius de Lima Wilson Levy Braga da silva neto Marília Marton Correia Thaísa Leal da silva Postgraduation Program of Architecture and Urbanism School of Engineering and Applied SciencesATITUS EducaçãoRua Senador Pinheiro304Passo Fundo/RS 99070-220Brazil Postgraduation Program in Smart and Sustainable Cities at Universidade Nove de Julho Rua Vergueiro235/249-LiberdadeSão Paulo/SP 01525-000Brazil

There is a growing worldwide interest in the potential of using existing buildings to generate their own energy as a way to alleviate problems related to the exhaustion of energy resources,environmental degradation and the constant increase in energy-related *** the distributed electricity generation solutions on the market today,wind energy can be a solution for many existing city buildings ***,the objective of this article is to investigate the economic viability of generating wind energy in a multifamily building located in Southern Brazil,(city of Passo Fundo),seeking to supply the condominium electric energy for the period of one year of consumption with a renewable *** this end,the energy demand of the condominium and the energy generation of the wind turbine were taken into account to calculate the economic viability of the *** results showed a positive NPV(net present value)demonstrating the economic feasibility of carrying out such an *** IRR(internal rate of return)was 15.22%and the return on investment(payback)occurs in 7.88 years.

关键词： Wind energy microgeneration renewable energy economic analysis

Use of Silicon in Mitigating Ammonium Toxicity in Maize Plants

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American Journal of Plant Sciences 2015年第11期6卷 1780-1784页

作者： Cid Naudi silva Campos Renato de Mello Prado Cassiano Garcia Roque Antonio Joao de Lima neto Luiz Junior Pereira Marques Aridênia Peixoto Chaves Claudia Amaral Cruz Department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Department of Soils and Fertilizers Universidade Estadual Paulista/FCAV Jaboticabal Brazil Universidade Federal de Mato Grosso do Sul Chapad&atildeo do Sul Brazil Universidade Federal de Viçosa Vi&ccedilosa Brazil Department of Applied Biology and Agriculture Universidade Estadual Paulista/FCAV Jaboticabal Brazil Department of Plant Science Universidade Federal Rural do Semi-árido Mossoró Brazil M.Sc. in Agronomy Natal Brazil

Silicon is a beneficial element that can mitigate abiotic stresses, such as ammonium toxicity. The objective herein was to evaluate the effects of silicon (Si) on mitigating toxicity caused by excess ammonium in maize plants grown in nutrient solution. An experiment was conducted with maize plants (cultivar DKB 390 VT Pro II) grown in a greenhouse in pots (8 L) in a hydroponic system. The treatments were arranged in a 2 × 2 factorial, consisting of two ammonium concentrations (30 and 60 mmol·L-1) in the absence and presence of Si (10 mmol·L-1), arranged in a completely randomized design with six repetitions. At 28 days after applying the treatments the dry mass of shoots and roots was evaluated, along with accumulation of silicon and nitrogen in the shoots. The use of silicon resulted in increases in the studied variables, regardless of ammonium concentrations. Silicon reduced the effect of toxicity caused by ammonium excess in maize plants, resulting in greater growth and dry matter accumulation.

关键词： Zea mays L. Beneficial Element Nitrogen Nutritional Disorder Abiotic Stress

Advances in cellular technology in the hematology field: What have we learned so far?

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World Journal of Stem Cells 2015年第1期7卷 106-115页

作者： Gustavo Torres de Souza Claudineia Pereira Maranduba Camila Maurmann de Souza danielle Luciana Aurora Soares do Amaral Francisco Carlos da Guia Rafaella de Souza Salomao Zanette Joao Vitor Paes Rettore Natana Chaves Rabelo Lucas Mendes Nascimento Icaro Franca Navarro Pinto Julia Boechat Farani Abrahao Elias Hallack neto Fernando de Sa silva Carlos Magno da Costa Maranduba Angelo Atalla Laboratory of Human Genetics and Cell Therapy Biology Department Biological Sciences Institute Federal University of Juiz de Fora Juiz de Fora 36036-900Brazil Haematology and Bone Marrow Transplantation Service Medical Clinic Department University Hospital Federal University of Juiz de Fora Juiz de Fora 36036-900 Brazil

Despite the advances in the hematology field, blood transfusion-related iatrogenesis is still a major issue to be considered during such procedures due to blood antigenic incompatibility. This places pluripotent stem cells as a possible ally in the production of more suitable blood products. The present review article aims to provide a comprehensive summary of the state-of-theart concerning the differentiation of both embryonic stem cells and induced pluripotent stem cells to hematopoietic cell lines. Here, we review the most recently published protocols to achieve the production of blood cells for future application in hemotherapy, cancer therapy and basic research.

关键词： Hematopoietic stem cells Progenitor Induced pluripotent stem cells Reprogramming Embryonic stem cells

Biomedical Perspectives About Women with Chronic Pelvic Pain:A Qualitative Analysis

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International Journal of Clinical Medicine 2012年第5期3卷 411-418页

作者： Paula P.Souza Adriana P.M.S.Romao Ana Marcia S.Nakano Julio C.Rosa-e-silva Francisco J.Candido-dos-Reis Antonio A.Nogueira Omero B.Poli-neto Department of Gynaecology and Obstetrics School of Medicine University HospitalUniversity of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil Department of Public Health School of NursingUniversity of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil School of Medicine University Hospital University of Sao Paulo at Ribeirao PretoRibeirao PretoBrazil

Because the aetiology of chronic pelvic pain is complex, studies of the condition involve extensive investigation but provide few conclusions. Numerous studies have addressed the experiences of women with chronic pelvic pain, as well as the interaction between those women and their health care providers. Our objective was to investigate how physicians at a specialised clinic perceive the medical care provided to such women. This was a qualitative study employing semi-structured interviews and content analysis. We interviewed seven physicians at the Chronic Pelvic Pain Outpatient Clinic of the University Hospital, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Brazil. Medical training and practice constituted the central theme of the study, which was subdivided into categories addressing the influence that the current medical training has on the type of medical care provided to women with chronic pelvic pain. Medical practice has been characterised by a reductionist approach to health and illness, as well as by the fragmentation of health care. These characteristics are, to a certain extent, the result of the biomedical model of education, which has been predominant, ignoring social, cultural, psychological and emotional aspects. There is a need to shift the medical paradigms toward a humanistic model of health care. We hope that we have provided a critical view of current medical training and practice, as well as of their effects in various health care settings, particularly in the provision of care to women with chronic pelvic pain.

关键词： Chronic Pelvic Pain Biomedical Model Qualitative Research

Comparison of Three Experimental Models for Rat Osteoarthritis Induction

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Journal of Biosciences and Medicines 2016年第12期4卷 62-69页

作者： Henrique Ribeiro Rodrigues-neto Edilson Ferreira Andrade-Junior Denilson Jose silva Feitosa-Junior André Lopes Valente Thiago Cesar Xavier Bianca Caroline do Nascimento Alho Renan Kleber Costa Teixeira Fabio Vidal Moriya Rui Sérgio Monteiro de Barros Department of Orthopaedics and Traumatology Porto Dias Hospital Bel&eacute m Brazil Experimental Surgery Laboratory Par&aacute State University Bel&eacute m Brazil

Background: Osteoarthritis is a slowly progressive and debilitating disease with high prevalence in adult population. Knee is one of the joints most affected by this disorder. There are several models for animals’ osteoarthritis induction, however it is not identified any paper that compares these techniques. The present study was aimed to define the most appropriate model for rats osteoarthritis induction. Material and Methods: 40 Wistar rats were distributed into 4 groups of 10 animals each: normality group (NG);meniscectomy group (MG);quinolone group (QG) and iodoacetate group (IG). Radiographic images of the rat’s knees were analyzed as well as the amount of chondrocytes in the epiphyseal and articular cartilage. Results: In the radiographic analysis, there was a low correlation between the raters. Regarding the amount of chondrocytes in the epiphyseal cartilage, it was noticed that the IG and QG groups had fewer chondrocytes than NG, in contrast to MG that reported similar results to normality (p > 0.05). There was no significant difference between IG and QG groups (p > 0.05). Regarding the amount of chondrocytes in articular cartilage, it was noticed that the IG group showed fewer chondrocytes than NG (p 0.05). There was no significant difference between QG and MG groups (p > 0.05). Conclusion: Intraarticular injection of iodoacetate in rats is the model with greatest effect on reduction of chondrocytes amount.

关键词： Osteoarthritis Knee Joint Experimental Arthritis Animal Disease Models Rats

A Rare Case of Diaphragmatic Extralobar Pulmonary Sequestration

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Open Journal of Medical Imaging 2017年第4期7卷 156-162页

作者： Francisco Barbosa De Araujo neto Raphael Gomes Teixeira Tiago Castello Branco Lyra Glaucio Rodrigo silva De Siqueira Marcos Duarte Guimaraes Stefanie Gallotti Borges Carneiro Vinícius Martins Valois Tiago Bezerra Albano Erick Falci Santos Department of Radiology at Hospital Heliopolis Sao PauloBrazil

Context: Bronchopulmonary sequestration is defined as a congenital malformation in which a portion of lung tissue does not communicate with the normal bronchial tree and the pulmonary arteries. Case Report: We present a case of a 35-year-old male complaining of left lower back pain and with a mass in the left thoracoabdominal transition. Imaging studies have suggested a pulmonary sequestration and its diagnosis was confirmed by histopathological study. Conclusion: Diaphragmatic extralobar pulmonary sequestration is a rare anomaly whose radiological diagnosis is difficult. This case is illustrative and demonstrates the importance of imaging methods to the correct diagnosis and effective treatment.

关键词： Respiratory System Abnormality Diaphragmatic Pulmonary Sequestration Extralobar