检索结果-南通市图书馆

Study and ICH validation of a reverse-phase liquid chromatographic method for the quantification of the intact monoclonal antibody cetuximab

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Pharmaceutical Analysis 2016年第2期6卷 117-124页

作者： antonio Martinez-Ortega Agustin Herrera antonio Salmeron-garcia Jose Cabeza Luis Cuadros-Rodriguez Natalia Navas Department of Analytical Chemistry University of Granada Faculty of Science Campus Fuentenueva s/n E-18071 Granada Spain UGC intercentro Interniveles Farmacia Granada San Cecilio Hospital Biomedical Research Institute ibs. GRANADA. Hospitales Universitarios de Granada University of Granada E-18012 Granada Spain Department of Analytical Chemistry Science Faculty Biomedical Research Institute ibis. GRANADA University of Granada Campus Fuentenuevo s/n E-18071 Granada Spain

Cetuximab （CTX） is a potent chimeric mouse/human monoclonal antibody （mAb） approved worldwide for treatment of metastatic colorectal cancer. Among the various biological and physical analyses per- formed for full study on this biopharmaceutic, the determination of the concentration preparations throughout manufacturing and subsequent handling in hospital is particularly relevant. In the present work, the study and validation of a method for quantifying intact CTX by reverse-phase high-perfor- mance liquid chromatography with diode array detection （（RP）HPLC/DAD） is presented. With that end, we checked the performance of a chromatographic method for quantifying CTX and conducted a study to validate the method as stability-indicating in accordance with the International Conference on Harmo- nization guidelines （ICH） for biotechnological drugs; therefore, we evaluated linearity, accuracy, preci- sion, detection and quantification limits, robustness and system suitability. The specificity of the method and the robustness of the mAb formulation against external stress factors were estimated by compre- hensive chromatographic analysis by subjecting CTX to several informative stress conditions. As de- monstrated, the method is rapid, accurate, and reproducible for CTX quantification. It was also suc- cessfully used to quantify CTX in a long-term stability study performed under hospital conditions.

关键词： Reverse-phase high performance liquidchromatography Diode detector Stress study Biopharmaceuticals Cetuximab

Is device-measured vigorous physical activity associated with health-related outcomes in children and adolescents? A systematic review and meta-analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2021年第3期10卷 296-307页

作者： antonio garcia-Hermoso Yasmin Ezzatvar Robinson Ramirez-Velez Jordi Olloquequi Mikel Izquierdo Navarrabiomed Navarra Hospital Complex(CHN)Public University of Navarra(UPNA)IdiSNAPamplona 31008Spain University of Santiago of Chile(USACH) Sciences of Physical ActivitySports and Health SchoolSantiago 71783-5Chile Exercise Intervention for Health Research Group(EXINH-RG) Department of PhysiotherapyUniversitat de ValenciaValencia 46010Spain Institute of Biomedical Sciences Faculthy of Health SciencesUniversidad Autonoma de ChileTalca 3460000Chile

Objective:This study sought to analyze the prospective association between vigorous-intensity physical activity(VPA)and health-related outcomes in children and ***:Studies reporting associations between device-measured VPA and health-related factors in children and adolescents aged 318 years were identified through database searches(MEDLINE,EMBASE,and SPORTDiscus).Correlation coefficients were pooled if outcomes were reported by at least 3 studies,using DerSimonian-Laird random effects ***:Data from 23 studies including 13,674 participants were pooled using random effects *** associations were found between VPA at baseline and overall adiposity(r=0.09,95%confidence interval(95%CI):0.15 to0.03;p=0.002;I^(2)=89.8%),cardiometabolic risk score(r=0.13,95%CI:0.24 to0.02,p=0.020;I^(2)=69.6%),cardiorespiratory fitness(r=0.25,95%CI:0.150.35;p<0.001;I^(2)=57.2%),and total body bone mineral density(r=0.16,95%CI:0.06 to 0.25;p=0.001;I^(2)=0%).Conclusion:VPA seems to be negatively related to adiposity and cardiometabolic risk score and positively related to cardiorespiratory fitness and total body bone mineral density among children and adolescents at ***,our findings support the need to strengthen physical activity recommendations regarding VPA due to its health benefits in children and adolescents.

关键词： Accelerometry Adiposity Fitness Physical activity

Ophiolite hosted chromitite formed by supra-subduction zone peridotite–plume interaction

在线全文

学校读者我要写书评

暂无评论

Geoscience Frontiers 2020年第6期11卷 2083-2102页

作者： Júlia Farré-de-Pablo Joaquín A.Proenza JoséMaría González-Jiménez Thomas Aiglsperger antonio garcia-Casco Javier Escuder-Viruete Vanessa Colás Francisco Longo Departament de Mineralogia Petrologia i Geologia AplicadaUniversitat de BarcelonaC/Martíi Franquess/n08028BarcelonaSpain Departamento de Mineraloga y Petrologa Universidad de GranadaFacultad de CienciasFuentenueva s/n18002GranadaSpain Department of Civil Engineering and Natural Resources LuleåUniversity of TechnologySE 97187LuleåSweden Instituto Andaluz de Ciencias de la Tierra(CSIC-UGR) Avda.de las Palmeras 4E-18100ArmillaGranadaSpain Instituto Geologico y Minero de Espana C/La Calera 1Tres Cantos28760MadridSpain Instituto de Geología Universidad Nacional Autonoma de MéxicoCiudad Universitaria04510Ciudad de MéxicoMexico Facultad de Ingeniería Universidad Catòlica de CibaoLa VegaDominican Republic

Chromitite bodies hosted in peridotites typical of suboceanic mantle(***)are found in the northern and central part of the Loma Caribe peridotite,in the Cordillera Central of the Dominican *** chromitites are massive pods of small size(less than a few meters across)and veins that intrude both dunite and ***,they are high-Cr chromitites[Cr#=Cr/(Cr+Al)atomic ratio=0.71-0.83]singularly enriched in TiO2(up to 1.25 wt.%),Fe2 O3(2.77-9.16 wt.%)as well as some trace elements(Ga,V,Co,Mn,and Zn)and PGE(up to 4548 ppb in whole-rock).This geochemical signature is unknown for chromitites hosted in oceanic upper mantle but akin to those chromites crystallized from mantle plume derived ***,the melt estimated to be in equilibrium with such chromite from the Loma Caribe chromitites is similar to basalts derived from different source regions of a heterogeneous Caribbean mantle *** mantle plume is responsible for the formation of the Caribbean Large Igneous Province(CLIP).Dolerite dykes with back-arc basin basalt(BABB)and enriched mid-ocean ridge basalt(E-MORB)affinities commonly intrude the Loma Caribe peridotite,and are interpreted as evidence of the impact that the Caribbean plume had in the off-axis magmatism of the back-arc basin,developed after the Caribbean island-arc extension in the Late *** propose a model in which chromitites were formed in the shallow portion of the back-arc mantle as a result of the metasomatic reaction between the supra-subduction zone(SSZ)peridotites and upwelling plume-related melts.

关键词： Chromitite Ophiolite Plume Mantle Back-arc basin Dominican Republic

Innovation, Competitiveness and Prosperity： A Factor Analysis of Innovation Indicators in Spain

在线全文

学校读者我要写书评

暂无评论

Management Studies 2018年第5期6卷 375-383页

作者： antonio garcia-Sanchez David Siles Maria de Mar Vazquez-Mendez Technical University of Cartagena Cartagena Spain

Competitiveness is a wide concept applied to many fields, especially in economics. The study of tourism competitiveness has been focused on different factors that can enhance the prosperity of a destination. One of these factors is innovation. Innovation makes destination＇s enterprises more advanced and efficient, therefore more productive. Innovation is an enhancer of competitiveness and a generator of prosperity because innovation in every aspect （technology, knowledge, organization, and processes） will provide a better quality of life for the inhabitants of the destination. The incidence of innovation on these concepts is validated by a structural equation model. We measure innovation by a range of indicators and through a factor analysis; we get the most relevant indicators of innovation for the model developed in Spain.

关键词： competitiveness innovation prosperity tourism factors indicators

Malignant Cerebral Edema Secondary to Gliadel Wafers in the Early Postsurgical Period

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Anesthesiology 2015年第4期5卷 72-74页

作者： Ana B. Fernández antonio garcia Eglis Lazo Department of Anesthesiology Intensive Care and Pain Treatment Nuestra Sra de Cande-laria University Hospital Santa Cruz de Tenerife Spain Department of Neurosurgery Nuestra Sra de Candelaria University Hospital Santa Cruz de Tenerife Spain

High grade gliomas are the commonest intrinsic brain tumours and account for more average years of life lost than all the common cancers. It has become the commonest cause of cancer death in men under the age of 45 and women under the age of 35. Although surgical resection can greatly reduce tumour bulk, complete excision is virtually impossible due to the infiltrative nature of these tumours. In an attempt to treat the infiltrating tumour cells, there has been much interest in using local therapies inserted at the time of surgery. The authors report a case of fatal cerebral edema unresponsive to aggressive medical and surgical assessment that finally evolved to premature death in the early postsurgical period, after the craniotomy and implantation of Gliadel wafers. They note that high doses of dexamethasone were insufficient to prevent cerebral edema and death. A search for corticosteroid use and dosing for patients treated with Gliadel wafers in the published literature revealed no recommendations on the doses of steroids to be administered. In our opinion this is a very important issue and maybe the key point for the treatment of this disease, and may need to be addressed with treatment guidelines in the near future in order to ensure better results on patient’s survival. Prior to this case review there had been two similar report but a later presentation. So we think that this is the first case report of acute fulminant cerebral edema secondary to gliadel wafers in the early period.

关键词： Malignant Glioma Carmustine Wafer Cerebral Edema Cytotoxic Effect

Games-Based Learning Framework

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computer Technology and Application 2011年第11期2卷 906-911页

作者： antonio garcia-Cabot Eva garcia Roberto Barchino Luis de-Marcos Jose-Maria Gutierrez Jose-antonio Gutierrez Jose-Javier Martinez Salvador Oton Jose-Ramon Hilera Computer Science Department University of Alcala Alcala de Henares Madrid 28871 Spain

This work presents the authors＇ experience in the field of mobile technologies, from which several initiatives have emerged. As result of this, a games-based framework for learning has been developed in these last years. This framework is composed by a competition called Mobigame, which has as main aim to stimulate the participation of the students. By participating in this competition participants learn to develop for mobile devices. A game to practice Japanese is also presented in this article, which was presented in the above mentioned competition. This game has been developed for mobile phones or PDAs （Personal Digital Assistants） based on the JME （Java Mobile Edition） technology. Finally, another initiative is also presented： A free download platform of digital contents for mobile devices based on info-educational games.

关键词： Game-based learning mobile framework.

Transportation Safety Requirements in the Use of Pushchairs on Buses

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Traffic and Transportation Engineering 2017年第2期5卷 63-76页

作者： Juan Dols Enrique Alcalá Jaime Masiā Luis Martinez Angel Martin antonio garcia Institute for Design and Manufacturing (IDF) Universitat Politècnica de Valencia Spain Camino de Vera s/n. 46022 Valencia Spain University Institute for Automobile Research (INSIA) Universidad Politécnica de Madrid Spain Campus sur de la UPM Ctra. de Valencia km Z 28031 Madrid Spain

The safe use of buses by passengers travelling with pushchairs for young children is a mobility problem that has not yet been satisfactorily resolved. The lack of national and international standards in this area in the past led to the development of a research project developed jointly by the Universitat Politecnica Valencia and the Universidad Politecnica Madrid （Spain）. This paper shows a comparative study to evaluate the dynamic behaviour in case of low-g accidents reproducing frontal impacts resembling real traffic events （deceleration 2g, AV 20 km/h） of a new pushchair restraint system against the use of back-restraint by pushchairs as establishes R 107- 06 series of amendments. In the dynamic trials, three pushchair restraint prototypes and a typical wheelchair back-restraint system combined with two worst-case up-to-date pushchair models in misuse and correct use configurations were tested. The results demonstrated the need for preventing children injuries as a consequence of low-g accidents. The new design meets and improves the latest revision of regulation R 107 regarding the use of restraint systems for the transport of wheelchair and pushchair passengers and represents an advance for pushchairs travelling on buses.

关键词： Transportation safety pushchairs children injury restraint system public transport.

Muscle strength cut-offs for the detection of metabolic syndrome in a nonrepresentative sample of collegiate students from Colombia

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2020年第3期9卷 283-290页

作者： antonio garcia-Hermoso Alejandra Tordecilla-Sanders Jorge Enrique Correa-Bautista Mark D.Peterson Mikel Izquierdo Aura Cristina Quino-ávila Carolina Sandoval-Cuellar Katherine González-Ruíz Robinson Ramíez-Vélez Physical Activity Sport and Health Sciences LaboratoryUniversity of Santiago de ChileSantiago de Chile 7500618Chile Center of Studies in Physical Activity Measurements School of Medicine and Health SciencesUniversidad del RosarioBogot a 111221Colombia Department of Physical Medicine and Rehabilitation University of MichiganAnn ArborMichigan 48109USA Department of Health Sciences Public University of NavarreCIBERFES(CB16/10/00315)Navarre 31009Spain CORPS Group Faculty of Health SciencesUniversity of BoyacáBoyac aTunja 153610Colombia Physical Exercise and Sports Research Group Manuela Beltrán UniversityBogot a 111221Colombia

Background: Evidence shows an association between grip strength and health;however, grip strength cut-offs for the detection of metabolic syndrome(MetS) in Latin American populations are scarce. The purpose of this study was to determine cut-offs of normalized grip strength(NGS)for the detection of MetS in a large nonrepresentative sample of a collegiate student population from ***: A total of 1795 volunteers(61.4% female;age = 20.68 ± 3.10 years, mean ± SD), ranging between 18 and 30 years of age participated in the study. Strength was estimated using a handheld dynamometer and normalized to body mass(handgrip strength(kg)/body mass(kg)). Anthropometrics, serum lipids indices, blood pressure, and fasting plasma glucose were measured. Body composition was measured by bioelectrical impedance analysis. MetS was defined as including ≥3 of the 5 metabolic abnormalities according to the International Diabetes Federation definition. A metabolic risk score was computed from the following components: waist circumference, triglycerides, high-density lipoprotein cholesterol, glucose, and systolic and diastolic blood ***: Receiver operating curve analysis showed significant discriminatory accuracy of NGS in identifying the thresholds and risk *** strength was associated with increased prevalence of MetS. In males, weak, intermediate, and strong NGS values at these points were0.615, respectively. In females, these cut-off points were 0.437, ***: Our sex-specific cut-offs of NGS could be incorporated into a clinical setting for identifying college students at cardiometabolic disease risk.

关键词： Cardiometabolic risk Muscular fitness Receiver operating characteristic curve Thresholds

A Calcium Paradox in the Context of Neurotransmission

在线全文

学校读者我要写书评

暂无评论

Journal of Pharmacy and Pharmacology 2015年第6期3卷 253-261页

作者： Leandro B. Bergantin Aron Jurkiewicz antonio G. garcia Afonso Caricati-Neto Department of Pharmacology Eseola Paulista de Medieina Universidade Federal de Sao Paulo Sao Paulo 04039-032 Brazil Department of Pharmacology Instituto Teofilo Hernando de I+ D del Medicamento Universidad Autonoma de Madrid Madrid 28029 Spain

The hypothesis of the calcium paradox has its origin in experiments done in neurogenically stimulated rat and mouse vas deferentia. Some old studies reported that reduction of Ca2＋ entry by mild concentrations of verapamil, diltiazem or nifedipine elicited the surprising augmentation of vas deferens contractions. Recent reports have also found that nifedipine caused a paradoxical augmentation of the exocytotic release of catecholamine elicited by paired depolarising pulses in voltage-clamped bovine chromaffin cells. Because these drugs are blocking the L-subtype of VACCs （voltage-activated calcium channels）, augmented contraction and exocytosis was an unexpected outcome. Recent experiments in neurogenically-stimulated rat vas deferens have found a more drastic potentiation of contractions with the association of verapamil and cAMP-enhancer compounds. Thus, the interaction between the signalling pathways mediated by Ca2＋ and cAMP could explain those unexpected findings and the so-called calcium paradox.

关键词： Calcium paradox cAMP smooth muscle sympathetic neurotransmission chromaffin cell.