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Surfactant protein A (SP-A) binds to phosphatidylserine and competes with annexin V binding on late apoptotic cells

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Protein & Cell 2010年第2期1卷 188-197页

作者： anne Jäkel Kenneth B.M.Reid Howard Clark Department of Pharmacology University of OxfordMansfield RoadOxford OX13QTUK MRC Immunochemistry Unit Department of BiochemistryUniversity of OxfordSouth Parks RoadOxford OX13QUUK University of Southampton MP 803 Level FSouth BlockSouthampton General HospitalSouthampton SO166YDUK

The role of surfactant protein A(SP-A)in the recognition and clearance of apoptotic cells is well established,but to date,it is still not clear which surface molecules of apoptotic cells are involved in the *** we present evidence that phosphatidylserine(PS)is a relevant binding molecule for human *** binding is Ca^(2+)-dependent and is not inhibited by mannose,suggesting that the sugar-binding site of the carbohydrate recognition domain(CRD)of SP-A is not *** cytometry studies on apoptotic Jurkat cells revealed apparent inhibition of annexin V binding by increasing concentrations of SP-A in late apoptotic but not early apoptotic cells,and this was consistent for Jurkat cells and *** these data,confocal microscopy results show a co-localisation of annexin V and SP-A in late apoptotic but not early apoptotic ***,we cannot conclude that this inhibition is exclusively due to the binding of SP-A to PS on the cell surface,as annexin V is not wholly specific for PS and SP-A also interacts with other phospholipids that might become exposed on the apoptotic cell surface.

关键词： surfactant proteins phosphatidylserine apoptotic cells

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Diversification of the RAB Guanosine Triphosphatase Family in Dicots and Monocots

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Journal of Integrative Plant Biology 2007年第8期49卷 1129-1141页

作者： Jiaming Zhang Daniel R. Hill anne W. Sylvester State Key Biotechnology Laboratory for Tropical Crops Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 571101 China Department of Molecular Biology 3994 1000 East University Ave. University of Wyoming Laramie Wyoming 82071 USA

RAB guanosine triphosphatases （GTPases） are key regulators of vesicle trafficking and are essential to the growth and development of all eukaryotic cells. During evolution, the RAB family has expanded in different patterns to facilitate distinct cellular, developmental and physiological adaptations. Yeast has only 11 family members, whereas mammalian RABs have expanded to 18 RAB subfamilies. Plant RABs have diversified primarily by duplicating members within a single subfamily. Plant RABs are divided into eight subfamilies, corresponding to mammalian RAB1, RAB2, RAB5, RAB6, RAB7, RAB8, RAB11 and RAB18. Functional diversification of these is exemplified by the RAB1 ls, orthologs of which are partitioned into unique cell compartments in plants where they function to transport vesicles during localized tip growth. Similarly, the RAB2 family in grasses is likely involved in vesicle secretion associated with wall expansion, as determined by analysis of over-expression mutants. We propose that dicots and monocots have also diverged in their RAB profiles to accommodate unique cellular functions between the two groups. Here we present a bioinformatics analysis comparing the RAB sub-families of rice, maize and Arabidopsis. These results will guide future functional studies to test for the role of diversification of subfamilies unique to monocots compared to dicots.

关键词： dicot GTP binding protein monocot phylogenetic analysis RAB guanosine triphosphatase

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The Chlamydomonas Chloroplast HLP Protein Is Required for Nucleoid Organization and Genome Maintenance

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Molecular Plant 2009年第6期2卷 1223-1232页

作者： Daniel Karcher Dietrich Koster anne Schadach Anja Klevesath Ralph Bock Max-Planck-lnstitut fur Molekulare Pflanzenphysiologie Am Muhlenberg 1 D-14476 Potsdam-Golm Germany

The chloroplasts genome （plastome） occurs at high copy numbers per cell. Several chloroplast genome copies are densely packed into nucleoprotein particles called nucleoids. How genome packaging occurs and which proteins organize chloroplast nucleoids are largely unknown. Here, we have analyzed the Chlamydornonas reinhardtii homolog of the bacterial architectural DNA-binding protein HU, the histone-like protein HLP. We show that the Chlarnydornonas HLP protein is targeted to chloroplasts and associates with nucleoids. Knockdown of HLP gene expression by RNA interference （RNAi） alters the structure of chloroplast nucleoids and appears to reduce the level of compaction of chloroplast DNA. Unexpectedly, also chloroplast genome copy numbers are significantly decreased in the RNAi strains, suggesting that, in addition to its architectural role in nucleoid formation, the HIP protein is also involved in chloroplast genome maintenance.

关键词： Chloroplast nucleoid Chlamydomonas reinhardtii HLP DNA-binding protein genome maintenance

Minimally invasive and open gallbladder cancer resections: 30-vs 90-day mortality

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Hepatobiliary & Pancreatic Diseases International 2017年第4期16卷 405-411页

作者： Naeem Goussous Motahar Hosseini anne M Sill Steven C Cunningham Department of Surgery Saint Agnes HospitalBaltimoreMDUSA

BACKGROUND:Minimally invasive surgery is increasingly used for gallbladder cancer *** mortality at 30 days is low,but 90-day mortality is ***:Using National Cancer Database(1998-2012),all resection patients were ***-and 90-day mortality rates were ***:A total of 36 067 patients were identified,19 139(53%) of whom underwent *** age was 71 years and 70.7% were ***-day mortality following surgical resection was 2.3-fold higher than 30-mortality(17.1% vs 7.4%).There was a statistically significant increase in 30-and 90-day mortality with poorly differentiated tumors,presence of lymphovascular invasion,tumor stage,incomplete surgical resection and low-volume centers(P<0.001 for all).Even for the 1885 patients who underwent minimally invasive resection between 2010 and 2012,the 90-day mortality was 2.8-fold higher than the 30-day mortality(12.0% vs 4.3%).CONCLUSIONS:Ninety-day mortality following gallbladder cancer resection is significantly higher than 30-day *** mortality is associated with tumor grade,lymphovascular invasion,tumor stage,type and completeness of surgical resection as well as type and volume of facility.

关键词： gallbladder cancer laparoscopic minimally invasive survival radical cholecystectomy complications

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Nuisance species:beyond the ecological perspective

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Ecological Processes 2014年第1期3卷 236-247页

作者： Zina Skandrani Sebastien Lepetz anne-Caroline Prevot-Julliard Centre d’Ecologie et des Sciences de la Conservation CESCO UMR 7204 CNRS-MNHN-UPMCMuseum National d’Histoire NaturelleCP 5155 rue BuffonF-75005 ParisFrance Laboratoire d’Archéozoologie et Archéobotanique UMR 7209 CNRS-MNHNMuseum National d’Histoire NaturelleCP 5155 rue BuffonF-75005 ParisFrance

Introduction:‘Nuisance species’(or‘invasive’species)are often proposed to be the second most important concern in the context of the current biodiversity *** increasing evidence that exotic species do not always become invasive,this perception is still common in the scientific *** suggests that other issues are at stake in the concept of nuisance species rather than just the problems they raise,grounded mostly in ecological or economic ***:We retraced the evolution of pigeon representation in France through an extensive review of ancient texts related to pigeons and reviewed more than 240 source texts,dating from the seventh century to the *** completed these data with literature on human-animal relationships and animal conceptions in religious,philosophical,scientific,and political currents of thought that were representative of Western and,specifically,French ***:We used the heated debate over pigeons(Columba livia)as pests as a case study by analyzing the ecological,social,economic,and political relations regarding *** a historical perspective of the debate about and understanding of pigeons,we propose a new and complementary explanation for the modern thinking of pigeons as a nuisance species,based on what we termed their socio-nature *** particular,we used social representations theory to highlight the issues of human identity construction at stake in the construction of pigeons as a nuisance ***:We invite the reader to consider the impact of such human self-definition on environmental stances,as it could constrain further developments or improvements of conservation perspectives.

关键词： Conservation Human-animal relations Identity construction ‘Nuisance species’ Social representations Socio-natures

Identification of a novel population of human cord blood cells with hema-topoietic and chondrocytic potential

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Cell Research 2004年第4期14卷 268-282页

作者： Karen E JAY,anne ROULEAU,T Michael UNDERHILL,Mickie BHATIA Stem Cell Biology and Regenerative Medicine The John P. Robarts Research Institute 100 Perth Drive London Ontario N6A 5K8 Canada Department of Microbiology and Immunology The University of Western Ontario London Ontario Canada Stem Cell Biology and Regenerative Medicine The John P. Robarts Research Institute 100 Perth Drive London Ontario N6A 5K8 Canada Department of Physiology The University of Western Ontario London Ontario Canada Stem Cell Biology and Regenerative Medicine The John P. Robarts Research Institute 100 Perth Drive London Ontario N6A 5K8 Canada Department of Microbiology and Immunology The University of Western Ontario London Ontario Canada. Department of Physiology The University of Western Ontario London Ontario Canada

With the exception of mature erythrocytes, cells within the human hematopoietic system are characterized by the cell surface expression of the pan-leukocyte receptor CD45. Here, we identify a novel subset among mononuclear cord blood cells depleted of lineage commitment markers (Lin) that are devoid of CD45 expression. Surprisingly, functional examination of Lin"CD45" cells also lacking cell surface CD34 revealed they were capable of multipotential hematopoietic progenitor capacity. Co-culture with mouse embryonic limb bud cells demonstrated that Lin-CD45-CD34- cells were capable of contributing to cartilage nodules and differentiating into human chondrocytes. BMP-4, a mesodermal factor known to promote chondrogenesis, significantly augmented Lin-CD45-CD34- differentiation into chondrocytes. Moreover, unlike CD34+ human hematopoietic stem cells, Lin-CD45-CD34- cells were unable to proliferate or survive in liquid cultures, whereas single Lin-CD45-CD34- cells were able to chimerize the inner cell mass (IC

关键词： hematopoietic chondrocyte CD45 embryonic.

The feasibility of coating by cooling crystallization on ibuprofen naked tablets

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Frontiers of Chemical Science and Engineering 2017年第2期11卷 211-219页

作者： Fatima Mameri Ouahiba Koutchoukali Mohamed Bouhelassa anne Hartwig Leila Nemdili Joachim Ulrich University Constantine 3 Engineering Faculty of Pharmaceutical Processes 25000 Constantine Algeria Martin Luther University Halle-Wittenberg Center of Engineering Science Thermal Process Engineering D-06099 Halle Germany

Sugar spray coating is a frequently used process in the pharmaceutical industry. However, this process presents the disadvantage to form an amorphous coating around the active ingredient. A crystalline coating formed on the surface of a tablet is highly desirable. Recently, a new process of coating by cooling crystallization has been developed and applied on bisacodyl pastilles obtained by melt crystallization. In this work, we investigated the feasibility of coating by cooling crystallization on ibuprofen ＂naked tablets＂ manufactured by compression. In the first part of this work, the solubility and the metastable zone width have been determined experimentally for the coating solution because they are essential factors for any crystallization process. In the second part, the coating process is investigated on the operating conditions that affect the surface morphology and the crystal growth rate. These experimental conditions include concentration of the coating solution, degree of sub-cooling, agitation speed, retention time, and surface properties of the naked ibuprofen tablets. The results show that naked tablet coating by cooling crystallization is feasible and can be applied in the pharmaceutical industry.

关键词： coating solution crystallization ibuprofen tablets sucrose

Unexpected Breg-NK crosstalk in STING agonist therapy

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Cellular & Molecular Immunology 2022年第12期19卷 1330-1332页

作者： Yunke Wang anne E.Geller Jun Yan Division of Immunotherapy The Hiram C.PolkJr.MD Department of SurgeryImmuno-Oncology ProgramBrown Cancer CenterUniversity of Louisville School of MedicineLouisvilleKYUSA Department of Microbiology and Immunology University of Louisville School of MedicineLouisvilleKYUSA

In a recent paper published in Nature,Li et *** that resistance to stimulator of interferon genes(STING)monotherapy in solid tumors is attributable to the unexpected surge of IL-35-producing regulatory B(Breg)cells that impair NK-cell cytotoxicity[1].They depict STING-induced Breg-NK-cell crosstalk and propose that combining the STING agonist 2'3'cyclic GMP-AMP(cGAMP)with anti-IL-35 blockade is a promising strategy to overcome tumor immunosuppression,especially in pancreatic cancers[1].

关键词： Breg interferon

Overview and detectability of the genetic modifications in ornamental plants

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Horticulture Research 2020年第1期7卷 2622-2633页

作者： anne-Laure Boutigny Nicolas Dohin David Pornin Mathieu Rolland Anses Plant Health LaboratoryBacteriology Virology GMO Unit7 rue Jean Dixméras49044 Angerscedex 01France

The market of ornamental plants is extremely competitive,and for many species genetic engineering can be used to introduce original traits of high commercial ***,very few genetically modified(GM)ornamental varieties have reached the market so ***,the authorization process required for such plants has a strong impact on the profitability of the development of such *** the numerous scientific studies using genetic modification on ornamental species of interest,a lot of transformed material has been produced,could be of commercial interest and could therefore be unintentionally released on the *** unintentional use of GM petunia in breeding programs has indeed recently been *** review lists scientific publications using GM ornamental plants and tries to identify whether these plants could be detected by molecular biology tools commonly used by control laboratories.

关键词： breeding ornamental lists