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Effect of exogenous fibrolytic enzymes on performance and blood profile in early and mid-lactation Holstein cows

Animal Nutrition 2015年第3期1卷 229-238页

作者： anja Peters Ulrich meyer Sven Danicke Institute of Animal Nutrition Friedrich-Loeffler-Institute(FLI)Federal Research Institute for Animal Health

The supplementation of exogenous fibrolytic enzymes(EFE) to dairy cows diets could be a strategy to improve fiber degradation in the rumen which is especially important for the early lactating cows characterized by a high milk energy output and an insufficient energy intake. The objective of this study was to examine the effects of a fibrolytic enzyme product(Roxazyme G2 Liquid, 3.8 and 3.9 mL/kg total mixed ration [TMR] DM) supplemented to a TMR on production performance and blood parameters of dairy cows during early(trial 1) and mid-lactation(trail 2). In addition, rumination activity was measured in trial 2. The nutrient digestibility of the experimental TMR was obtained by using wethers. In the digestibility trial, EFE was supplemented at a rate of 4.4 m L/kg Roxazyme G2 Liquid TMR-DM. The TMR contained 60% forage and 40% concentrate(DM basis). Twenty eight 50 ± 16 days in milk(DIM)and twenty six 136 ± 26 DIM Holstein cows were used in two 8-wk completely randomized trails,stratified by parity and milk yield level. One milliliter of the enzyme product contained primarily cellulase and xylanase activities(8,000 units endo-1,4-? glucanase, 18,000 units endo-1,3(4)-? glucanase and 26,000 units 1,4-? xylanase). No differences in digestibility of DM, OM, CP, NDF and ADF were observed(P >0.05) between the control and the EFE supplemented TMR. Addition of EFE to the TMR fed to early(trial 1) and mid-lactation cows(trial 2) did not affect daily dry matter intake(DMI), milk yield,4% fat-corrected milk, energy-corrected milk(ECM), concentration of milk fat, protein, fat-protein-quotients, somatic cell score, energy balance, and gross feed efficiency of early and mid-lactation cows(P >0.05). Mid-lactation cows(trial 2) fed with TMR enzyme showed a tendency of a slightly higher ECM yield(P = 0.09). The tested blood parameters were not affected by treatment in trials 1 and 2(P >0.05).Exogenous fibrolytic enzymes supplementation did not alter daily time spent ruminating in t

关键词： Fibrolytic enzymes Dairy cows Milk yield Feed efficiency

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Demonstrating grating-based phase-contrast imaging of laser-driven shock waves

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Matter and Radiation at Extremes 2024年第4期9卷 90-97页

作者： Leonard Wegert Stephan Schreiner Constantin Rauch Bruno Albertazzi Paulina Bleuel Eric Frojdh Michel Koenig Veronika Ludwig Artem SMartynenko Pascal meyer Aldo Mozzanica Michael Müller Paul Neumayer Markus Schneider Angelos Triantafyllidis Bernhard Zielbauer Gisela Anton Thilo Michel Stefan Funk Plasma Physics Department GSI Helmholtzzentrum fur SchwerionenforschungPlanckstraBe 164291 DarmstadtGermany Erlangen Centre for Astroparticle Physics(ECAP) Friedrich-Alexander-Universität Erlangen-NurnbergNikolaus-Fiebiger-StraBe 291058 ErlangenGermany LULI-CNRS CEASorbonne UniversitésEcole PolytechniqueInstitut Polytechnique de ParisF-91120 Palaiseau CedexFrance Paul Scherrer Institut(Psi) Forschungsstrasse 1115232VilligenSwitzerland Karlsruhe Institute of Technology Institute of Microstructure TechnologyHermann-von-Helmholtz-Platz 176344 Eggenstein-LeopoldshafenGermany

Single-shot X-ray phase-contrast imaging is used to take high-resolution images of laser-driven strong shock *** a two-grating Talbot interferometer,we successfully acquire standard absorption,differential phase-contrast,and dark-field images of the shocked *** agreement is demonstrated between experimental data and the results of two-dimensional radiation hydrodynamics simulations of the laser-plasma *** main sources of image noise are identified through a thorough assessment of the interferometer’s *** acquired images demonstrate that grating-based phase-contrast imaging is a powerful diagnostic tool for high-energy-density *** addition,we make a novel attempt at using the dark-field image as a signal modality of Talbot interferometry to identify the microstructure of a foam target.

关键词： interaction shock phase

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无弹性物质参与的密封是未来的趋势

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流程工业 2024年第4期 14-17页

作者： Harry Jost anja Quattelbaum Matthias Dörr NEUMO GmbH+Co.KG

即将实施的PFAS(全氟和多氟烷基化合物)禁令以及更严格的TA Luft(空气清洁技术标准)排放限值,是当前困扰着化工和生命科学行业从业者的两大热门话题。这使得管道连接如何能够实现安全密封并符合法规要求成为焦点问题。本文描述了制药、... 详细信息

即将实施的PFAS(全氟和多氟烷基化合物)禁令以及更严格的TA Luft(空气清洁技术标准)排放限值,是当前困扰着化工和生命科学行业从业者的两大热门话题。这使得管道连接如何能够实现安全密封并符合法规要求成为焦点问题。本文描述了制药、生物科技、食品和化工及氢能领域在这样的政策环境下所面临的问题,并给出了解决方案。此外,本文还特别介绍了不含弹性密封材料的管道连接件,并强调了无弹性物质参与的密封是未来的趋势。

关键词：连接件密封交叉污染

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Single-cell transcriptome atlases of soybean root and mature nodule reveal new regulatory programs that control the nodulation process

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Plant Communications 2024年第8期5卷 69-91页

作者： Sergio Alan Cervantes-Perez Prince Zogli Sahand Amini Sandra Thibivilliers Sutton Tennant Md Sabbir Hossain Hengping Xu Ian meyer Akash Nooka Pengchong Ma Qiuming Yao Michael J.Naldrett Andrew Farmer Olivier Martin Samik Bhattacharya Jasper Klaver Marc Libault Department of Agronomy and Horticulture University of Nebraska-LincolnLincolnNE 68503USA Division of Plant Science and Technology College of AgricultureFoodand Natural ResourcesUniversity of Missouri-ColumbiaColumbiaMO 65211USA Interdisciplinary Plant Group of Missouri-Columbia ColumbiaMO 65211USA School of Computing University of Nebraska-LincolnLincolnNE 68503USA Proteomics and Metabolomics Facility Center for BiotechnologyUniversity of Nebraska-LincolnLincolnNE 68588USA National Center for Genome Resources Santa FeNM 87505USA INRAE UniversiteParis-SaclayInstitut des Sciences des Plantes de Paris SaclayIPS2Batiment 630 Plateau du MoulonRue Noetzlin91192 Gif sur Yvette CedexFrance Resolve Biosciences Monheim am RheinGermany Present address:The School of Plant Sciences University of ArizonaTucsonAZ 85721USA Present address:GALY Co. 100 Hood Park Dr.BostonMA 02126USA

The soybean root system is *** addition to being composed of various cell types,the soybean root system includes the primary root,the lateral roots,and the nodule,an organ in which mutualistic symbiosis with N-fixing rhizobia occurs.A mature soybean root nodule is characterized by a central infection zone where atmospheric nitrogen is fixed and assimilated by the symbiont,resulting from the close cooperation between the plant cell and the *** date,the transcriptome of individual cells isolated from developing soybean nodules has been established,but the transcriptomic signatures of cells from the mature soybean nodule have not yet been *** single-nucleus RNA-seq and Molecular Cartography technologies,we precisely characterized the transcriptomic signature of soybean root and mature nodule cell types and revealed the co-existence of different sub-populations of ***-infected cells in the mature soybean nodule,including those actively involved in nitrogen fixation and those engaged in *** of the single-cell-resolution nodule transcriptome atlas and the associated gene co-expression network confirmed the role of known nodulation-related genes and identified new genes that control the nodulation *** instance,we functionally characterized the role of GmFWL3,a plasma membrane microdomain-associated protein that controls rhizobial *** study reveals the unique cellular complexity of the mature soybean nodule and helps redefine the concept of cell types when considering the infection zone of the soybean nodule.

关键词： soybean root nodule single-cell RNA-seq nanodomains

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乐活健康

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空中英语教室（高级版．彭蒙惠英语） 2024年第1期 10-11,39,40页

作者： Patrick McCreless John meyer 不详

03一名医生从自己的中风体验中学到的三件事或许能拯救你的生命当朱利安·金医生正在示范一个小型外科手术时,他走路开始出现异常。他自己没有注意到,但手术室里有人注意到了“当时我脑出血了……和高血压有关,”金医生说,“我甚至不知... 详细信息

03一名医生从自己的中风体验中学到的三件事或许能拯救你的生命当朱利安·金医生正在示范一个小型外科手术时,他走路开始出现异常。他自己没有注意到,但手术室里有人注意到了“当时我脑出血了……和高血压有关,”金医生说,“我甚至不知道我有高血压。”

关键词：外科手术高血压手术室脑出血医生朱利安

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Application of Electron Beam Surface Technologies in the Automotive Industry

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材料热处理学报 2004年第5期25卷 573-578页

作者： Rolf Zenker anja Buchwalder TU Bergakademie Freiberg /Zenker Consult Mittweida GermanyTU Bergakademie Freiberg Germany

Progress in the beam deflection technique opens up new possibilities for the application of electron beam (EB) surface and welding technologies in the automotive industry. This development is based on three-dimensional high-speed beam deflection and fully automatic online process control. So, in the EB surface treatment three-dimensional energy transfer fields can be realised which take into account the contour of a component, the conditions of heat conduction and the load conditions. High flexibility, precision and reproducibility are typical characteristics. High productivity is achieved by the simultaneous interaction of the EB in several processing areas or by carrying out several processes simultaneously. EB surface treatment is becoming more and more attractive and important especially in the automotive industry, and also in comparison to laser technologies. This paper deals with different EB surface technologies, for example hardening, remelting, surface alloying, dispersing or cladding of different materials such as steel, cast iron and different alloys of Al, Mg and Ti. Examples of applications in the automotive industry, especially engine components, will be discussed.

关键词：电子注铸铁 3D射束偏移表面热处理

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Insights into manganese transport: A matter of phosphorylation

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Molecular Plant 2022年第3期15卷 385-387页

作者： anja Schneider Plant Science Faculty of BiologyLudwig-Maximilians-Universität MünchenMunichGermany

Manganese(Mn)is an essential element required for the growth of all known living *** plants acquire Mn from the rhizosphere,and its translocation into,and distribution within,the plant involves a multitude of transporters(Alejandro et al.,2020).In the aerial part of the green plant,the most prominent reaction that depends on Mn is the water-splitting reaction of photosystem II,which employs a tetra-Mn cluster located in the thylakoid lumen of ***,no less important reactions take place in mitochondria,where a Mn superoxide dismutase acts as a scavenger of reactive oxygen species,and in the Golgi apparatus,where Mn-dependent glycosyltransferases are involved in cell wall biosynthesis.

关键词： manganese apparatus splitting

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Progenies of NG2 glia: what do we learn from transgenic mouse models ?

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Neural Regeneration Research 2021年第1期16卷 43-48页

作者： Qilin Guo anja Scheller Wenhui Huang Molecular Physiology Center for Integrative Physiology and Molecular Medicine(CIPMM)University of SaarlandHomburgGermany

In the mammalian central nervous system,nerve-glia antigen 2(NG2)glia are considered the fourth glial population in addition to astrocytes,oligodendrocytes and *** fate of NG2 glia in vivo has been carefully studied in several transgenic mouse models using the Cre/loxP *** is a clear agreement that NG2 glia mainly serve as progenitors for oligodendrocytes and a subpopulation of astrocytes mainly in the ventral forebrain,whereas the existence of a neurogenic potential of NG2 glia is lack of adequate *** mini review summarizes the findings from recent studies regarding the fate of NG2 glia during *** will highlight the age-and-region-dependent heterogeneity of the NG2 glia differentiation *** will also discuss putative reasons for inconsistent findings in various transgenic mouse lines of previous studies.

关键词： astrogliogenesis cell fate Cre/loxP system development differentiation embryonic brain neurogenesis NG2 glia oligodendrocyte lineage oligodendrocyte precursor cells

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Oligodendroglial GABAergic Signaling:More Than Inhibition!

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Neuroscience Bulletin 2021年第7期37卷 1039-1050页

作者： Xianshu Bai Frank Kirchhoff anja Scheller Molecular Physiology Center for Integrative Physiology and Molecular Medicine(CIPMM)University of Saarland66421 HomburgGermany

GABA is the main inhibitory neurotransmitter in the CNS acting at two distinct types of receptor:ligand-gated ionotropic GABAA receptors and G protein-coupled metabotropic GABAB receptors,thus mediating fast and slow inhibition of excitability at central *** signal transmission has been intensively studied in neurons in contrast to oligodendrocytes and their precursors(OPCs),although the latter express both types of GABA *** studies focusing on interneuron myelination and interneuron-OPC synapses have shed light on the importance of GABA signaling in the oligodendrocyte *** this review,we start with a short summary on GABA itself and neuronal GABAergic ***,we elaborate on the physiological role of GABA receptors within the oligodendrocyte lineage and conclude with a description of these receptors as putative targets in treatments of CNS diseases.

关键词： GABA GABAA receptor GABAB receptor OPC Oligodendrocyte lineage

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MLMT与MLST在新生隐球菌基因分型中的应用研究

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贵州医科大学学报 2024年第1期49卷 84-89页

作者：朱键曹佳非康颖倩三上襄 Wieland meyer 贵阳市第二人民医院&贵州医科大学附属金阳医院消化内科贵州贵阳550081 贵州医科大学附属医院消化内科贵州贵阳550004 贵州医科大学研究生院贵州贵阳550004 千葉大学医学真菌研究中心日本千葉县260-8673 悉尼大学Westmead医学研究所澳大利亚悉尼NSW2145

目的探讨多位点微卫星灶分型(MLMT)和多位点序列分型(MLST)在新生隐球菌(***)基因分型中的应用。方法提取22株来源于国内及日本已明确为新生隐球菌的菌株DNA,经MLMT及MLST相应位点的引物进行扩增、测序;计算各微卫星灶(CNG1、CNG2及CN... 详细信息

目的探讨多位点微卫星灶分型(MLMT)和多位点序列分型(MLST)在新生隐球菌(***)基因分型中的应用。方法提取22株来源于国内及日本已明确为新生隐球菌的菌株DNA,经MLMT及MLST相应位点的引物进行扩增、测序;计算各微卫星灶(CNG1、CNG2及CNG3)序列中简单重复序列的重复数,确定菌株MLMT的基因型;将本研究菌株的MLST中各位点序列和网上数据库相应序列进行对比,获取该菌株的MLST基因型;比较两种方法在分型中分辨能力。结果在22株菌中,有16株的基因型为MLMT17/MLST5(16/22,72.73%),MLMT将所有菌株分为7型,而MLST分为4型;根据MLST分型,MLMT17、16、34及39的菌株为MLST5;MLMT2、14、29相对应MLST66、32及31;两种方法的分型分辨能力比较,差异无统计学意义(P=0.488)。结论 MLMT-17/MLST 5是我国和日本的新生隐球菌常见基因型,包含CNG1、CNG2及CNG3位点的MLMT可作为MLST的替代或补充。

关键词：新生隐球菌多位点微卫星灶分型多位点序列分型

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