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Case Study for Undetermined Mosquito Species by Polymerase Chain Reaction in Western Burkina Faso

American Journal of Molecular Biology 2024年第2期14卷 43-53页

作者： Koudraogo Bienvenue Yaméogo Sassan Simplice Kambou Domonbabélé François de Sales Hien Logotio Rachel angela Traore Franck Adama Yao Rakiswendé Serge Yerbanga Centre National de la Recherche Scientifique et Technologique CNRST Institut de Recherche en Sciences de la Sante (IRSS) Laboratoire de recherche sur les maladies infectieuses et parasitaires Bobo-Dioulasso Burkina Faso Institut des Sciences et Techniques (INSTech Bobo) Avenue de Sya Bobo-Dioulasso Burkina Faso

Introduction: Malaria eradication campaigns all over the world are largely based on parasite and vector control. Vector identification, whether morphological or molecular, is an essential component of vector control. This study analyzed the possible causes of indeterminate polymerase chain reaction (PCR) results for mosquito species in Western part of Burkina Faso. Methodology: From July 2021 to November 2021, mosquitoes were collected during the period of high malaria transmission in the village of Séguéré, Houet province, Burkina Faso, and morphologically identified. After DNA extraction, samples were amplified by sine 200× PCR to identify species of the Anopheles gambiae complex. Indeterminate samples were then selected for further analysis. The parameters studied were: DNA dilution, the effect of protocol adjusting, and the type of protocol used. Results: A total of 130 “indeterminate” DNAs diluted 1:10 were analyzed. After dilution, the mean amount was 14.73 ± 3.59 ng/μL and absorbance 1.71 ± 0.1. PCR chain reaction yielded 94.62% (123/130) anopheline species in SINE PCR, 5.38% (7/130) “negative”. A significant difference between SINE PCR before dilution and after dilution was observed (P < 0.001). Identification tests carried out using other protocols gave no positive results. From these results, we note that the adaptation of the protocol significantly reduced the polymerase amplification results of the species. Conclusion: It is therefore necessary to respect the amplification protocols. However, the persistence of “indeterminate” results suggests that further studies should be carried out to shed more light on the subject.

关键词： Malaria Vector DNA PCR

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Dysfunction of the adhesion G protein-coupled receptor latrophilin 1(ADGRL1/LPHN1)increases the risk of obesity

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2298-2311页

作者： Heba ZabriTorsten Schöneberg AndréNguyen Dietzsch Hadi Al-Hasani Joachim Altschmied Katharina Bottermann Jana Brendler Judith Haendeler Susanne Horn Isabell Kaczmarek Antje Körner Kerstin Krause Kathrin Landgraf Diana Le Duc Laura Lehmann Stefan Lehr Stephanie Pick Albert Ricken Rene Schnorr angela Schulz Martina Strnadová Akhil Velluva Heba Zabri Torsten Schöneberg Doreen Thor Simone Prömel Rudolf Schönheimer Institute of Biochemistry Medical FacultyLeipzig UniversityLeipzigGermany Institute for Clinical Biochemistry and Pathobiochemistry Medical FacultyGerman Diabetes CenterLeibniz Center for Diabetes Research at Heinrich Heine University DüsseldorfDüsseldorfGermany German Center for Diabetes Research(DZD e.V.) Munich-NeuherbergGermany Cardiovascular Degeneration Clinical Chemistry and Laboratory DiagnosticsMedical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Cardiovascular Research Institute(CARID) Medical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Institute of Pharmacology Medical FacultyUniversity Hospital and Heinrich Heine University DüsseldorfDüsseldorfGermany Institute of Anatomy Medical FacultyLeipzig UniversityLeipzigGermany Center for Pediatric Research Hospital for Children and AdolescentsMedical FacultyLeipzig UniversityLeipzigGermany Helmholtz Institute for Metabolic Obesity and Vascular Research(HI-MAG)of the Helmholtz Zentrum München at the University of Leipzig and University Hospital LeipzigLeipzigGermany Department of Endocrinology NephrologyRheumatologyLeipzig University Medical CenterLeipzigGermany Institute of Human Genetics Leipzig University Medical CenterLeipzigGermany Institute of Cell Biology Department of BiologyHeinrich Heine University DüsseldorfDüsseldorfGermany School of Medicine University of Global Health EquityKigaliRwanda

Obesity is one of the diseases with severe health consequences and rapidly increasing worldwide *** the complex network of food intake and energy balance regulation is an essential prerequisite for pharmacological intervention with obesity.G protein-coupled receptors(GPCRs)are among the main modulators of metabolism and energy ***,for instance,regulate appetite and satiety in certain hypothalamic neurons,as well as glucose and lipid metabolism and hormone secretion from *** in some GPCRs,such as the melanocortin receptor type 4(MC4R),have been associated with early-onset ***,we identified the adhesion GPCR latrophilin 1(ADGRL1/LPHN1)as a member of the regulating network governing food intake and the maintenance of energy *** of the highly conserved receptor in mice results in increased food consumption and severe obesity,accompanied by dysregulation of glucose ***,we identified a partially inactivating mutation in human ADGRL1/LPHN1 in a patient suffering from ***,we propose that LPHN1 dysfunction is a risk factor for obesity development.

关键词： obesity metabolism intake

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全产业链视角下美国游戏产业发展趋势观察

中国数字出版

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中国数字出版 2024年第5期2卷 101-106页

作者：唐玙萱北京市第二中学

文章将数字时代的游戏作为一种全球性媒介，着眼于全产业链视角，深入探讨美国游戏产业在内容生产、技术应用与传播三大环节的最新趋势，以期为未来游戏产业发展提供有益参考。从内容生产环节来看，通过增强文本的多元叙事与互动参与升... 详细信息

文章将数字时代的游戏作为一种全球性媒介，着眼于全产业链视角，深入探讨美国游戏产业在内容生产、技术应用与传播三大环节的最新趋势，以期为未来游戏产业发展提供有益参考。从内容生产环节来看，通过增强文本的多元叙事与互动参与升维玩家认知实践；在技术应用层面来看，以制作技术和硬件技术的两手创新提升产品沉浸式体验；在营销发行环节来看，围绕平台建设一个核心助力游戏产品与服务的“破圈”传播。

关键词：美国游戏产业产业链技术创新平台

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Prehospital transdermal glyceryl trinitrate for ultra- acute ischaemic stroke: data from the RIGHT- 2 randomised sham- controlled ambulance trial

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Stroke & Vascular Neurology 2024年第1期9卷 38-49,I0035-I0046页

作者： Jason Philip Appleton Lisa J Woodhouse Craig S Anderson Sandeep Ankolekar Lesley Cala Mark Dixon Timothy J England Kailash Krishnan Grant Mair Keith W Muir John Potter Christopher I Price Marc Randall Thompson G Robinson Christine Roffe Else C Sandset Jeffrey L Saver angela Shone Aloysius Niroshan Siriwardena Joanna M Wardlaw Nikola Sprigg Philip M Bath Stroke University Hospitals Birmingham NHS Foundation TrustBirminghamUK Institute of Applied HealthResearch University of Birmingham College of Medical and Dental SciencesBirminghamUK Stroke Trials Unit Mental Health and Clinical NeurosciencesUniversity of NottinghamNottinghamUK Faculty of Medicine The George Institute for Global HealthSydneyNew South WalesAustralia The George Institute China at Peking University Health Science Center BeijingChina Neurology Royal Prince Alfred HospitalSydney Health PartnersSydneyNew South WalesAustralia Department of Neurology King's College Hospital NHS Foundation TrustLondonUK Faculty of Health and Medical Sciences University of Western AustraliaCrawleyWestern AustraliaAustralia East Midlands Ambulance Service NHS Trust NottinghamUK Stroke Queen's Medical CentreNottingham University Hospitals NHS TrustNottinghamUK Centre for Clinical Brain Sciences Dementia Research InstituteUniveristy of EdinburghEdinburghUK Institute of Neurology and Psychology University of GlasgowGlasgowUK Bob Champion Research and Education Building University of East AngliaNorwichUK Institute of Neuroscience Newcastle UniversityNewcastle upon TyneUK Department of Neurology Leeds Teaching Hospitals NHS TrustLeedsUK Department of Cardiovascular Sciences and NIHR Leicester Biomedical Research Centre University of LeicesterLeicesterUK Stroke Research in Stoke Institute for Science and Technology in MedicineKeele UniversityStoke-on TrentUK Department of Neurology Oslo University HospitalOsloNorway Research and Development Norwegian Air Ambulance FoundationOsloNorway Department of Neurology and Comprehensive Stroke Center David Geffen School of MedicineUCLALos AngelesCaliforniaUSA Research and Graduate Services University of NottinghamNottinghamUK Community and Health Research Unit University of LincolnLincolnUK

Background The effect of transdermal glyceryl trinitrate(GTN,a nitrovasodilator)on clinical outcome when administered before hospital admission in suspected stroke patients is ***,we assess the safety and efficacy of GTN in the prespecified subgroup of patients who had an ischaemic stroke within the Rapid Intervention with Glyceryl trinitrate in Hypertensive stroke Trial-2(RIGHT-2).Methods RIGHT-2 was an ambulance-based multicentre sham-controlled blinded-endpoint study with patients randomised within 4hours of *** primary outcome was a shift in scores on the modified Rankin scale(mRS)at day *** outcomes included death;a global analysis(Wei-Lachin test)containing Barthel Index,EuroQol-5D,mRS,telephone interview for cognitive status-modified and Zung depression scale;and neuroimaging-determined‘brain frailty’*** were reported as n(%),mean(SD),median[IQR],adjusted common OR(acOR),mean difference or Mann-Whitney difference(MWD)with 95%*** 597 of 1149(52%)patients had a final diagnosis of ischaemic stroke;age 75(12)years,premorbid mRS>2107(18%),Glasgow Coma Scale 14(2)and time from onset to randomisation 67[45,108]***‘brain frailty’was common:median score 2[2,3](range 0–3).At day 90,GTN did not influence the primary outcome(acOR for increased disability 1.15,95%CI 0.85 to 1.54),death or global analysis(MWD 0.00,95%CI-0.10 to 0.09).In subgroup analyses,there were non-significant interactions suggesting GTN may be associated with more death and dependency in participants randomised within 1hour of symptom onset and in those with more severe *** In patients who had an ischaemic stroke,ultra-acute administration of transdermal GTN in the ambulance did not improve clinical outcomes in a population with more clinical and radiological frailty than seen in previous in-hospital *** IS ALREADY KNOWN ON THIS TOPIC⇒Transdermal glyceryl trinitrate(GTN)was associat-ed with less death and dependency in those w

关键词： Glasgow nitrate random

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Role of lipid rafts in liver health and disease

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World Journal of Gastroenterology 2011年第20期17卷 2520-2535页

作者： angela Dolganiuc Department of Medicine University of Massachusetts Medical School

Liver diseases are an increasingly common cause of morbidity and mortality; new approaches for investigation of mechanisms of liver diseases and identification of therapeutic targets are emergent. Lipid rafts (LRs) are specialized domains of cellular membranes that are enriched in saturated lipids; they are small, mobile, and are key components of cellular architecture, protein partition to cellular membranes, and signaling events. LRs have been identified in the membranes of all liver cells, parenchymal and non-parenchymal; more importantly, LRs are active participants in multiple physiological and pathological conditions in individual types of liver cells. This article aims to review experimental-based evidence with regard to LRs in the liver, from the perspective of the liver as a whole organ composed of a multitude of cell types. We have gathered up-to-date information related to the role of LRs in individual types of liver cells, in liver health and diseases, and identified the possibilities of LR-dependent therapeutic targets in liver diseases.

关键词： Hepatocytes Stellate cells Kupffer cells Endothelial cells Signaling Therapeutic Viral HepatitisC virus Metabolism

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A head-to-toe makeover for classical sequencing-by-synthesis helps users to squeeze more out of each base

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National Science Review 2019年第1期6卷 3-4页

作者： Jianbin Wang angela Wu School of Life Sciences Tsinghua University Division of Life Science Hong Kong University of Science and Technology Department of Chemical and Biological Engineering Hong Kong University of Science and Technology

Today, many next-generation sequencing (NGS) methods, technologies and platforms have been developed that allow users to produce Tera base pairs of data in less than one day or ultralong reads covering multiple genes. By far the most popular NGS approach to date is cyclic sequencing-by-synthesis (SBS),whereby each DNA template’s complementary strand is synthesized by DNA polymerase and, as the new strand grows base by base, the identity of each

关键词： A head-to-toe makeover for classical sequencing-by-synthesis helps users to squeeze more out of each base

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In vitro and in vivo models of acute alcohol exposure

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World Journal of Gastroenterology 2009年第10期15卷 1168-1177页

作者： angela Dolganiuc Gyongyi Szabo Department of Medicine University of Massachusetts Medical School 364 Plantation Street Worcester MA 01605-2324 United States

Alcohol abuse is a global problem due to the financial burden on society and the healthcare system. While the harmful health effects of chronic alcohol abuse are well established, more recent data suggest that acute alcohol consumption also affects human wellbeing. Thus, there is a need for research models in order to fully understand the effect of acute alcohol abuse on different body systems and organs. The present manuscript summarizes the interdisciplinary advantages and disadvantages of currently available human and non-human models of acute alcohol abuse, and identifi es their suitability for biomedical research.

关键词： Models Acute alcohol abuse Human Nonhuman Progress

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Small is beautiful–a glycolytic metabolite signals mTORC1 activation in cancer cell metabolism

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Signal Transduction and Targeted Therapy 2020年第1期5卷 299-300页

作者： angela M.Otto Munich Institute of BioEngineering Technical University of Munich85748 GarchingGermany

Even though the mTOR complex has been known for decades as a nutrient sensor,being thus in focus for drug targeting in metabolic disease and cancer metabolism,it has remained puzzling how it is able to get a sense of glucose availability in ***,a small metabolite of the glycolytic pathway,dihydroxyacetone phosphate,has been identified by Sabatini and his coworkers,who show how mTORC1-activity is regulated in glucose-starved and replenished cancer cells.

关键词： metabolism cancer mTORC1

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How Africa Is Promoting Agricultural Innovations and Technologies amidst the COVID-19 Pandemic

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Molecular Plant 2020年第10期13卷 1345-1346页

作者： angela Joseph Fernando Consultant-The Alliance of Bioversity International and CIAT Km 17 Recta Cali-PalmiraApartado Aéreo 6713763537 CaliColombia

The COVID-19 pandemic has left an indelible mark on Africa's Agribusiness *** of households whose livelihoods depend on farming are facing a huge food *** mitigate these effects,the African Agricultural Technology Foundation(AATF)gathered a panel of renowned agricultural experts through an interactive webinar themed"Promotion of Agricultural Technologies and Innovations for Agri-Business Resilience in Africa in the Wake of COVID-19",the first in a series of several interactive seminars to be held virtually until the end of December *** seminars will focus on several topics specific to agricultural transformation,which revolve around smallholder farmers in Sub-Saharan Africa(SSA)and aim to help them come out of the cataclysmic effect of COVID-19 and recover their business and livelihoods.

关键词： Agricultural Africa interactive

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EZH2, an epigenetic driver of prostate cancer

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Protein & Cell 2013年第5期4卷 331-341页

作者： Yeqing angela Yang Jindan Yu Division of Hematology/Oncology Department of MedicineNorthwestern University Feinberg School of MedicineChicagoIL 60611USA Robert H.Lurie Comprehensive Cancer Center Northwestern University Feinberg School of MedicineChicagoIL 60611USA

The histone methyltransferase EZH2 has been in the limelight of the fi eld of cancer epigenetics for a decade now since it was fi rst discovered to exhibit an elevated expression in metastatic prostate *** persists to attract much scientifi c attention due to its important role in the process of cancer development and its potential of being an effective therapeutic *** here we review the dysregulation of EZH2 in prostate cancer,its function,upstream regulators,downstream effectors,and current status of EZH2-targeting *** review there-fore provides a comprehensive overview of EZH2 in the context of prostate cancer.

关键词： EZH2 histone methyltransferase prostate cancer epigenetics

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