A head-to-toe makeover for classical sequencing-by-synthesis helps users to squeeze more out of each base

作     者：Jianbin Wang Angela Wu 

作者机构：School of Life Sciences Tsinghua University Division of Life Science Hong Kong University of Science and Technology Department of Chemical and Biological Engineering Hong Kong University of Science and Technology 

出 版 物：《National Science Review》 (国家科学评论(英文版))

年 卷 期：2019年第6卷第1期

页      面：3-4页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

主　　题：A head to toe makeover for classical sequencing by synthesis helps users to squeeze more out of each base 

摘      要：Today, many next-generation sequencing (NGS) methods, technologies and platforms have been developed that allow users to produce Tera base pairs of data in less than one day or ultralong reads covering multiple genes. By far the most popular NGS approach to date is cyclic sequencing-by-synthesis (SBS),whereby each DNA template’s complementary strand is synthesized by DNA polymerase and, as the new strand grows base by base, the identity of each