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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 37-45页

作者： Yinyuan WU Da XING Lei LIU Tongsheng chen and wei r.chen moe key laboratory of laser life science &institute of life science,South china {3. university,guangzhou 51063.,china moe key laboratory of {3. life science and institute of {3. lifescience South China Normal UniversityGuangzhou 510631China

Ultraviolet (UV) irradiation is a DNA-damaging agent that triggers apoptosis through both themembrane death receptor and mitochondrial apoptotic signaling ***,a pro-apoptotic Bcl-2family member,is important in most cell types to apoptosis in response to DNA *** this study,arecombinant plasmid,YFP-Bid-CFP,comprised of yellow and cyan fluorescent protein and a full length Bid,was used as a fluorescence resonance energy transfer analysis (FrET) *** the FrET techniquebased on YFP-Bid-CFP,we found that Bid activation was initiated at 9±1 h after UV irradiation,and theaverage duration of the activation was 75±10 *** activation coincided with a collapse of the mitochondrialmembrane potential with an average duration of 50±10 min. When cells were pretreated with Z-IETD-fmk(caspase-8 specific inhibitor) the process of Bid activation was completely inhibited,but the apoptosis wasonly partially affected.Z-DEVD-fmk (caspase-3.inhibitor) and Z-FA-fmk (non asp specific inhibitor) didnot block Bid ***,the endogenous Bid activation with or without Z-IETD-fmk in responseto UV irradiation was confirmed by Western *** summary, using the FrET technique,we observedthe dynamics of Bid activation during UV-induced apoptosis and found that it was a caspase-8 dependentevent.

关键词： ultraviolet irradiation Bid activation apoptosis caspase-8 fluorescence resonance energy transfer analysis

Single Cell Analysis of Bid Activation in Living Cells during UV-induced Apoptosis

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Single Cell Analysis of Bid Activation in Living Cells durin...

Quantitative measurement of FrET efficiency free from spectral cross-talks in living cells

The 3.d Asian and Oceanian Conference on Photobiology

作者： Yinyuan Wu Da Xing Lei Liu and Tongsheng chen moe key {3. of laser life science & institute of laser life science South china normal {13. guangzhou 51063. china

＜正＞Bid is a proapoptotic member of the BH3.only subfamily of the Bcl-2 proteins, and is important for most cell types to apoptose in response to DNA damage. In this study, using a combination of single cell analysis and Fluorescence Spectroscopy techniques, we investigated the dynamics of Bid activation during UV-induced apoptosis. A recombinant plasmid YFP-Bid-CFP comprised of cyan and yellow fluorescent protein and a full length Bid, was used as FrET probe. Using FrET technique based on YFP-Bid-CFP, we found that Bid activation was initiated at 8-10 h after UV irradiation, and reached maximum activation in 1 h. Furthermore, when cells were pretreated with caspase-8 specific inhibitor （Z-IETD-fmk）. the process of Bid activation was completely inhibited. In summary, using FrET technique, we observed the dynamics of Bid activation during UV-induced apoptosis and it was a caspase-8 dependent event. FrET technique was an effective method to study protein activation in living cells during apoptosis.

关键词： UV irradiation Bid activation apoptosis caspase-8 single cell analysis

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Quantitative measurement of FRET efficiency free from spectr...

SINGLE CELL IMAGING OF BAX TrANSLOCATION DUrING APOPTOSIS INDUCED BY PHOTOFrIN-PDT

中国光学学会2011年学术大会

作者： Hua-Li Li,Tong-Sheng chen moe key {3. of laser life science & institute of laser life science,College of Biophotonics,South china normal {13.,guangzhou 51063.,china

Fluorescence resonance energy transfer（FrET） technology in combination with microscopic fluorescence imaging has been widely used for the investigation of interand intramolecular interaction in living *** recent years,many quantitative algorithms of FrET efficiency have been ***,they mainly focus on the elimination of excitation spectral crosstalk and the emission spectral crosstalk of *** report developed a method to quantitatively detect the FrET efficiency of a donor-acceptor pair in living *** can directly obtain the FrET efficiency of the donor-acceptor pair without any verified and control ***,this method is free from all the spectral cross-talks including the excitation and emission spectral bleedthrough of donor and *** used this method to detect the FrET efficiency of ECFP-EYFP in single living cells,and our data is consistent with that obtained by using fluorescence lifetime（FLIM） and Pb-FrET measurement.

关键词： Fluorescence resonance energy transfer(FrET) Donor-acceptor pair Crosstalk Photobleaching

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Journal of Innovative Optical Health sciences 2009年第2期2卷 209-214页

作者： FEIFAN ZHOU DA XING wei r.chen moe key laboratory of {3. life science&institute of {3. life science South china normal university Guangzhou 510631China Department of Engineering and Physics College of Mathematics and Science University of Central OklahomaEdmondOklahoma 73034USA

Apoptosis is an important cellular event that plays a key role in the therapy of many *** mechanism of the initiation and regulation of photodynamic therapy(PDT)–induced apoptosis is *** previous study found that Photofrin was localized primarily in mitochondria,the primary targets of *** key role of Bax in the mitochondria-mediated apoptosis has been demonstrated in many *** order to determine the role of Bax in the mitochondrion-mediated apoptosis induced by Photofrin-PDT,we used the GFP-Bax plasmid to monitor the dynamics of Bax activation after PDT *** laser scanning confocal microscopy,we found that Bax did not translocate from the cytosol to mitochondria when the mitochondrial membrane potential(∆Ψm)disappeared,measured by ***,for Photofrin-PDT,the commitment to cell death is independent of Bax activation.

关键词： Photofrin-photodynamic therapy mitochondria-mediated apoptosis Bax activation translocation.

Observation of transition from superfluorescence to polariton condensation in CsPbBr3.quantum dots film

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Light(science & Applications) 2024年第2期13卷 316-324页

作者： Danqun Mao Linqi chen Zheng Sun Min Zhang Zhe-Yu Shi Yongsheng Hu Long Zhang Jian Wu Hongxing Dong wei Xie Hongxing Xu State key laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200241China key laboratory of Materials for High-Power laser Shanghai Institute of Optics and Fine MechanicsChinese Academy of Sciences201800 ShanghaiChina Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuanShanxi 030006China Chongqing key laboratory of Precision Optics Chongqing Institute of East China Normal UniversityChongqing 401121China CAS Center for Excellence in Ultraintense laser science Shanghai 201800China School of Physics and Technology Center for Nanoscience and NanotechnologyWuhan UniversityWuhan 430072China

The superfluorescence effect has received extensive attention due to the many-body physics of quantum correlation in dipole gas and the optical applications of ultrafast bright radiation field based on the cooperative quantum ***,we demonstrate not only to observe the superfluorescence effect but also to control the cooperative state of the excitons ensemble by externally applying a regulatory dimension of coupling light fields.A new quasi-particle called cooperative exciton-polariton is revealed in a light-matter hybrid structure of a perovskite quantum dot thin film spin-coated on a Distributed Bragg *** the nonlinear threshold,polaritonic condensation occurs at a nonzero momentum state on the lower polariton branch owning to the vital role of the synchronized *** phase transition from superfluorescence to polariton condensation exhibits typical signatures of a decrease of the linewidth,an increase of the macroscopic coherence as well as an accelerated radiation decay *** findings are promising for opening new potential applications for super-brightness and unconventional coherent light sources and could enable the exploitation of cooperative effects for quantum optics.

关键词： quantum condensation polar

基于空域评价和分布统计的高功率激光装置近场状态分析方法

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中国激光 2024年第14期51卷 87-99页

作者：陈伟范薇杨琳中国科学院上海光学精密机械研究所高功率激光物理联合实验室上海201800 中国科学院大学材料科学与光电研究中心北京100049 上海交通大学IFSA协同创新中心上海200240

惯性约束聚变高功率激光驱动器优质的近场分布是提升装置输出性能的关键。研究了高功率激光装置基频近场状态的分析方法,利用统计光学理论,获得了近场通量分布的概率密度函数及其近似表达式,近场的统计特性反映出其整体分布特征及其变... 详细信息

惯性约束聚变高功率激光驱动器优质的近场分布是提升装置输出性能的关键。研究了高功率激光装置基频近场状态的分析方法,利用统计光学理论,获得了近场通量分布的概率密度函数及其近似表达式,近场的统计特性反映出其整体分布特征及其变化原因,由于信号通量和噪声通量可以用于描述理想的光束分布及扰动,近场分布信噪比能更显著地反映光束受扰动影响的程度;提出并验证了局部对比度和局部调制度的应用可行性,即能够定位近场异常区域并分析其状态演化;通过近场光束的通量统计研究了高通量点的演化过程,提供怀疑发生损伤的区域,并对高通量点与信噪比之间的指数模型提出了对比度的控制要求。研究结果为装置在高功率激光辐照下的安全运行提供了理论基础和实验支撑,提出了新的近场状态评估方法,对装置维护和运行策略调整起到一定的指导作用。

关键词：高功率激光驱动器近场光束质量分布统计空域评价近场分布信噪比

Switchable K-O bonds unveiling anomalous ferroelastic transitions in a polar hybrid crystal

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science china Materials 2024年第10期67卷 3408-3414页

作者： Yao-Bin Li wei-Jian Xu Xiao-Xian chen De-Xuan Liu Zhi-Shuo Wang wei-Xiong Zhang moe key laboratory of Bioinorganic and Synthetic Chemistry School of ChemistryIGCMESun Yat-sen UniversityGuangzhou 510275China Department of Chemistry&CICECO-Aveiro institute of Materials University of AveiroAveiro 3810-193Portugal

Hybrid ferroelastic crystals have garnered considerable interest due to their promising potential as mechanical switches and *** anomalous ferroelastic phase transitions,in which ferroelasticity occurs in the hightemperature phase rather than the low-temperature phase,are of particular interest,but they are sporadically-documented and none of them is involved in breaking of the chemical ***,a hydroxyl-containing cation,i.e.,Me_(3.NOH^(+),is employed to construct a three-dimensional hybrid crystal(Me_(3.NOH)_(2)KBiCl_(6)(1).This crystal undergoes distinct twostep structural phase transitions with space-group changes of Pna21–P112_(1)–P63.c,belonging to an anomalous temperaturereversed mm2F2 ferroelastic transition and a normal 6mmF2 ferroelastic transition,*** anomalous ferroelastic transition is entirely driven by switchable K–O coordination bonds involving breaking and ***,the dynamic behavior of Me_(3.NOH^(+)cations along with the distortion of inorganic framework enables the manifestation of unusual“high-low-medium”second-harmonic generation-switching *** study presents the enormous benefits of switchable coordination bonds for inducing anomalous ferroelastic phase transitions,offering valuable insight for the exploration of new multifunctional ferroelastic materials.

关键词： bond-switching transition ferroelastic phase transition hybrid crystal

ANTI-TUMOr rESPONSES INDUCED BY laser IrrADIATION AND IMMUNOLOGICAL STIMULATION USING A MOUSE MAMMArY TUMOr MODEL

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Journal of Innovative Optical Health sciences 2013年第4期6卷 49-55页

作者： FEIFAN ZHOU XIAOSONG LI SHENG SONG JOSEPH T.ACQUAVIVA III rOMAN F.WOLF ErIC W.HOWArD wei r.chen moe key laboratory of {3. life science&institute of {3. life science South china normal university {13. P.R.China Department of Oncology The First A±liated Hospital of Chinese PLA General Hospital Beijing 100048 P.R.China Department of Engineering and Physics university of Central Oklahoma Edmond OklahomaUSA Department of Comparative Medicine College of Medicine University of Oklahoma Health Sciences Center 940 S.L.Young Blvd.Oklahoma City OklahomaUSA Department of Cell Biology university of Oklahoma Health sciences Center 940 S.L.Young Blvd. Oklahoma City OklahomaUSA

Anti-tunor immunological response induced by local intervention is ideal for treatment of metastatic *** immunot herapy was developed to synergize photot hermal interaction with immunological stimulation for cancer *** an infrared laser,indocyanine green(ICG,as a light abeorbing agent),and glycated chitosan(GC,as an immunostimulant),.laser imm unot herapy has resulted in tumor suppression and anti-tumor responses in pre-clinical as well as clinical *** further understand the mechanism of laser immunotherapy,the efects of laser and GC treatment without specifc enhancement of laser absorption were *** adoptive immunity transfer was perfomed using splenocytes as immune *** cells harvested from tumor-bearing mice treated by laser+GC provided 60%immunity in naive ***,cytotoxicity and TNF-ar secretion by splenocytes from treated mice also indicated that laser+GC induced immunity was *** high level of infiltrating T cells in tumors after laser+GC treatment furt her confirned a specific anti-tumor immune ***,laser+GC could prove to be a promisi ng selective local trea tment modality that induces a systemic anti-tumor response,with appropriate laser parameters and GC doses.

关键词： laser immunotherapy glycated chitosan anti-tumor immunity metastatic cancers

Prospect of ultrahigh-resolution fast neutron absorption spectroscopy based on a laser plasma electron accelerator

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High Power laser science and Engineering 2024年第1期12卷 108-115页

作者： Wen-Zhao Wang Jie Feng Xiao-Peng Zhang Yao-Jun Li wei-Jun Zhou Wen-Chao Yan Guo-Qiang Zhang Chang-Bo Fu Li-Ming chen key laboratory of laser Plasma({3.) School of Physics and AstronomyShanghai Jiao Tong UniversityShanghaiChina IFSA Collaborative Innovation Center Shanghai Jiao Tong UniversityShanghaiChina institute of High Energy Physics Chinese Academy of SciencesBeijingChina Shanghai Advanced research institute Chinese Academy of SciencesShanghaiChina key laboratory of Nuclear Physics and Ion-beam Application(moe) Institute of Modern PhysicsFudan UniversityShanghaiChina

Fast neutron absorption spectroscopy is widely used in the study of nuclear structure and element analysis. However,due to the traditional neutron source pulse duration being of the order of nanoseconds, it is difficult to obtain a high-resolution absorption spectrum. Thus, we present a method of ultrahigh energy-resolution absorption spectroscopy via a high repetition rate, picosecond duration pulsed neutron source driven by a terawatt laser. The technology of single neutron count is used, which results in easily distinguishing the width of approximately 20 keV at 2 MeV and an asymmetric shape of the neutron absorption peak. The absorption spectroscopy based on a laser neutron source has one order of magnitude higher energy-resolution power than the state-of-the-art traditional neutron sources, which could be of benefit for precisely measuring nuclear structure data.

关键词： fast neutron absorption spectroscopy laser plasma accelerator photo-nuclear neutrons single neutron count

Gas microchannel plate-pixel detector for X-ray polarimetry

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Nuclear science and Techniques 2024年第5期35卷 60-73页

作者： Huan-Bo Feng Hong-Bang Liu Dong Wang Zi-Li Li Shu-Lin Liu Qian Liu Hang-Zhou Li Bin-Long Wang Yan-Jun Xie Zong-Wang Fan Hui Wang ran chen Di-Fan Yi rui-Ting Ma Fei Xie Bo Peng Xiang-Ming Sun Jin Li En-wei Liang Present Address:Guangxi key laboratory for relativistic Astrophysics School of Physical Science and TechnologyGuangxi UniversityNanning 530004China PLAC Key Laboratory of Quark and Lepton Physics(MOE)Central China Normal UniversityWuhan 430079China School of science Huzhou UniversityHuzhou 313000China institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China School of Nuclear science and Technology University of Chinese Academy of SciencesBeijing 100049China School of Physical science University of Chinese Academy of SciencesBeijing 100049China The 13.h research institute of china Electronics Technology Group Corporation Shijiazhuang 050057China

POLAr-2 is a gamma-ray burst(GrB)polarimeter that is designed to study the polarization in GrB radiation emissions,aiming to improve our knowledge of related ***-2 is expected to utilize an on-board polarimeter that is sensitive to soft X-rays(2-10 keV),called low-energy polarization *** have developed a new soft X-ray polari-zation detector prototype based on gas microchannel plates(GMCPs)and pixel chips(Topmetal).The GMCPs have bulk resistance,which prevents charging-up effects and ensures gain stability during *** detector is composed of low outgassing materials and is gas-sealed using a laser welding technique,ensuring long-term stability.A modulation factor of 41.28%±0.64% is obtained for a 4.5 keV polarized X-ray beam.A residual modulation of 1.96%±0.58% at 5.9 keV is observed for the entire sensitive area.

关键词： X-ray polarimetry Gas microchannel plate-pixel detector Gamma-ray bursts