SINGLE CELL IMAGING OF BAX TRANSLOCATION DURING APOPTOSIS INDUCED BY PHOTOFRIN-PDT

作     者：FEIFAN ZHOU DA XING WEI R.CHEN 

作者机构：MOE Key Laboratory of Laser Life Science&Institute of Laser Life Science South China Normal UniversityGuangzhou 510631China Department of Engineering and PhysicsCollege of Mathematics and Science University of Central OklahomaEdmondOklahoma 73034USA 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2009年第2卷第2期

页      面：209-214页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(30627003,30870676,30870658) the Natural Science Foundation of Guangdong Province(7117865) as well as by a grant from the US National Institute of Health(P20 RR016478 from the INBRE Program of the National Center for Research Resources) 

主　　题：Photofrin-photodynamic therapy mitochondria-mediated apoptosis Bax activation translocation. 

摘      要：Apoptosis is an important cellular event that plays a key role in the therapy of many *** mechanism of the initiation and regulation of photodynamic therapy(PDT)–induced apoptosis is *** previous study found that Photofrin was localized primarily in mitochondria,the primary targets of *** key role of Bax in the mitochondria-mediated apoptosis has been demonstrated in many *** order to determine the role of Bax in the mitochondrion-mediated apoptosis induced by Photofrin-PDT,we used the GFP-Bax plasmid to monitor the dynamics of Bax activation after PDT *** laser scanning confocal microscopy,we found that Bax did not translocate from the cytosol to mitochondria when the mitochondrial membrane potential(∆Ψm)disappeared,measured by ***,for Photofrin-PDT,the commitment to cell death is independent of Bax activation.