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Detection and Characterization of Non-covalent Complex between Lappaconitine and β-Cyclodextrin by Electrospray Ionization Tandem Mass Spectrometry

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Chinese Chemical Letters 2006年第1期17卷 65-68页

作者： Qing Xuan XU Li LI Hao YUE Zhi Qiang liu Ming Quart GUO shu ying liu Laboratory of New Drug Research and Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022

The non-covalent complexes between lappaconitine （LA） and β-cyclodextrin （β-CD） have been detected and characterized by electrospray ionization combined with ion trap tandem mass spectrometry （ESI-MSn）. The experimental results showed that only 1：1 non-covalent complex can be formed in different starting molar ratios of LA to β-CD. Furthermore, the diagnostic fragmentation of the β-CD-LA complex, with a significant contribution of covalent fragmentation of LA leaving the N-acetyl anthranoyl （AN） moiety inserted to β-CD, provided the convincing evidence for the formation of non-covalent complex between LA and β-CD and the cite of LA molecule included to cavity of β-CD assigned to AN residue.

关键词： Lappaconitine β-cyclodextrin non-covalent complex ESI-MS CID.

A STUDY OF DOUBLY CHARGED ION MASS SPECTRA(2E SPECTRA) OF BIPHENYL DERIVATIVES

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Chinese Chemical Letters 1991年第5期2卷 397-398页

作者： Feng He QIU shu ying liu Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun130022

The 2E spectra and substituent effects and target gas pressure dependence of the 2E spectra of 7 biphenyl derivatives were discussed. The electron-donating groups favour the molecular ions, but the electron withdrawing groups favour the fragaent ions produced by losing the substituents from molecular ions. The variation of target gas pressure affects the TIC,sample ion current,and the ratio of sample ion current to TIC *** it has no effect on the fragmentation pattern.

关键词： ION A STUDY OF DOUBLY CHARGED ION MASS SPECTRA OF BIPHENYL DERIVATIVES SPECTRA

Investigation of the Structures of the Product Ions Produced via self-CI of Acetone

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Chinese Chemical Letters 1998年第7期9卷 675-678页

作者： Zhi Li LI shu ying liu(Changchun Institute of Applied Chendstry, Chinese Academy of Sciences,Changchun 130022) Changchun Inst. of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

The ion-molecule reactions in acetone were investigated which were induced under the chemical ionization. The structural information of the reaction products were obtained by using collision-induced dissociation (CID)... 详细信息

关键词： ion-molecule reaction acetone ion structure collision-induced dissociation

Expression of Endogenous Beta Retroviruses and Hyal-2 mRNA in Immune Organs of Fetuses and Lambs

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Virologica Sinica 2012年第2期27卷 83-92页

作者： Jing-wei Qi Xiao-li Wu shu-ying liu Gui-fang Cao College of Animal Science and Veterinary Medicine Inner Mongolia Agricultural UniversityHuhhot 010018China Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease Ministry of AgricultureHuhhot 010018China

Endogenous beta retroviruses (enJSRV) are highly homologous with Jaagsiekte sheep retrovirus (exJSRV),this exogenous retrovirus is the aetiological agent of ovine pulmonary adenocarcinoma (OPA). The aim of this study was to clarify the function of enJSRV and the immunological mechanisms of its corresponding antibody, that is undetectable in JSRV-infected ovine serum. The expression of enJSRV envelope protein and Hyal-2 mRNA in immune organs and lungs of ovine fetuses and lambs were analyzed by Real-Time reverse transcription PCR and In Situ Hybridization using specific probes. In Situ Hybridization results indicated that the enJSRV envelope protein and Hyal-2 mRNA were expressed in thymus, spleen, mesenteric lymph nodes and lungs at different times, while no positive signals were detected in the negative controls. On the other hand, results from Real-Time reverse transcription PCR analysis showed that in 130d fetuses and 3d newborn lambs the enJSRV mRNA levels were much higher in organs associated with the immune system than that in lungs, especially in the thymus and spleen, but levels of Hyal-2 mRNA expression was not significantly different in all collected tissue. These results provided evidence from an immunology point of view to understand why the circulating antibodies against exJSRV are undetectable in JSRV-infected ovine, and will help to unravel the pathogenesis of JSRV-infected ovine.

关键词： Ovine enJSRV Hyal-2 In Situ Hybridization Real-Time PCR

Cloning and Sequence Analysis of Genome from the Inner Mongolia Strain of the Endogenous Betaretroviruses (enJSRV)

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Virologica Sinica 2008年第1期23卷 15-24页

作者： Yu WANG shu-ying liu Jian-yun LI Min HAN Zhen-ling WANG College of Animal Science and Animal Medicine Inner Mongolia Agricultural University Huhhot 010018China Beijing Vocational College of Agriculture Beijing 102442 China

In order to amplify the complete genome of enJSRV from the strain of Inner Mongolia (enJSRV-NM), we used enJSRV-specific and JSRV-specific DNA probes in dot blot hybridization. Seven pairs of primers were designed based on Genbank sequences. Seven fragments were obtained by PCR and were cloned into the PMD19-T vectors. The recombinant plasmids were sequenced and analyzed. The results showed that the genome was 7 942 bp in length and contained four overlapping open reading frames corresponding to the gag, pro, pol and env genes as well as an additional open reading frame (orf-x) that overlaps the 3' end of the pol gene. The nucleotide acid sequences of the enJSRV-NM loci were compared with the sequences of South Africa enJS56A1 strain (Accession No. AF153615) and USA JSRV21 strain (Accession No. AF105220). The nucleotide acid identities were 99.2% and 92.3% respectively. Two zinc fingers were found in the NC region in the predicted amino acid sequence. However, the YXXM motif, which is a reliable molecular marker for the infectious exogenous virus, was not found in the TM region. It was found that the enJSRV-NM region was 90%-98% identical at the amino acid level to its exogenous infectious counterparts in most of the retroviral genome. This is the first nucleotide sequence of enJSRV reported in P.R China. The resource work has provided a wide range of information useful not only for expression genomics and annotation of genomic DNA sequence, but also for further research on the clinical diagnosis of OPA.

关键词： Jaagsiekte sheep retrovirus (JSRV) Endogenous betaretroviruses (enJSRV) Ovine pulmonaryadenocarcinoma (OPA) Dot blot hybridization

High-Yield Synthesis and Extraction of Endohedral Metallofullerenes Dy@C_(2n)

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Chinese Chemical Letters 1997年第1期8卷 31-34页

作者： Da Yong SUN Zi Yang liu Xing Hua GUO Wen Guo XU shu ying liu(Changchun Institute of Applied Chemistry Chinese Academy of Sciences, Changchun 130022) Changchun Inst. of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

The successful encapsulation of dysprosium(Dy) into fullerene cages by activating the Dy2O3, containing graphite rods in situ 'back-burning' carbonarc evaporation with a high-yield of pyridine extraction technique is ... 详细信息

关键词： Dy Nature High-Yield Synthesis and Extraction of Endohedral Metallofullerenes Dy@C high

Fragmentation Reactions of Adduct Ions [M+CH_3CO]^+ of Isomeric Phenylenediamines

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Chinese Chemical Letters 1999年第10期10卷 833-834页

作者： Zhi Li LI shu ying liu Long Sheng LONG (1 6 Lab, Shandong Non-Metallic Material Research Institute, Jinan 2500312 Chanchun institute of Applied Chemistry, the Chinese Academy of Sciences, Jilin 1300223 Center of Analysis and Computation, China Pharmaceu 6 Lab. Shandong Non-Metallic Mat. Res. I. Jinan 250031 China Chanchun Inst. of Applied Chemistry Chinese Academy of Sciences Jilin 130022 China Center of Analysis and Computation China Pharmaceutical University Nanjing 210009 China

The dissociation routes of the adduct ions [M+CH3CO]+ formed by ion-moleculereaction of isomeric phenylenediamines with acetyl ion from acetone under chemical ionizationcondition were investigated by using collision-induced dissociation (CID) technique performed ation kinetic energies of 40eV. The adduct ions are intermediate ion-neutral complexes.

关键词： Collision-induced dissociation phenylenediamine ion-neutral complex.

Intensity Exaggeration of Endohedral Metallofullerenes in Laser Desorption-Ionization Time-of-Flight Mass Spectrometry

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Chinese Chemical Letters 1998年第8期9卷 761-764页

作者： Da Yong SUN Wen Guo XU and shu ying liu(The No. 9 Laboratory, Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022) No. 9 Laboratory Changchun Inst. of Applied Chemistry Chinese Academy of Sciences Changchun 13022 China

The laser-desorption-ionization time-of-flight mass spectrometry and desorption-electron-ionization mass spectrometry were employed for the characterization of metallofullerenes extract. it was found that the relative intensities of metallofullerenes in this positive-ion, negative-ion LD-TOF MS and DEI MS were much different. This phenomenon should have relationship with the peculiar ionization energies and electron affinities of metallofullerenes.

关键词： metallofullerenes LD-TOF MS

A　NEW　METHOD　FOR　THE　PREPARATION　OF　FULLEROL

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Chinese Chemical Letters 1996年第5期7卷 431-432页

作者： Da Yong SUN Zi Yang liu Xing Hua GUO Yi Min SHE Yu ZHOU shu ying liu (The Key Laboratory of Applied Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun. Jilin 130022) Key Lab. of Applied Spectroscopy Changchun Inst. of Applied Chemistry Chinese Academy of Sciences Changchun Jilin 130022

Abstract: Reaction of C60 with excessive potassium in toluene at refluxing temperature results in black precipitate K0C60 with high conversion. which can completely be hydrolyzed to prepare the fullerol. Investigation...

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