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The first protein ever synthesized in vitro—a personal reminiscence of the total synthesis of crystalline insulin

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Science china(Life sciences) 2010年第1期53卷 16-18页

作者： ZHANG YouShang institute of biochemistry and cell biology,shanghai institutes for biological sciences,Chinese Academy of sciences shanghai 200031,china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

The total synthesis of crystalline bovine insulin started in 1958 was fully accomplished in 1965. This formidable task was a collaboration of the institute of biochemistry in shanghai,

关键词： The first protein ever synthesized in vitro a personal reminiscence of the total synthesis of crystalline insulin

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Cloning and Analyzing of Xenopus Mespo Promoter in Retinoic Acid Regulated Mespo Expression

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Acta Biochimica et Biophysica Sinica 2006年第11期38卷 759-764页

作者： Jin-Hu WANG Xiao-Yan DING* institute of biochemistry and cell biology,shanghai institutes for biological sciences,Chinese Academy of sciences,Graduate School of the Chinese Academy of sciences,shanghai 200031,china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China

During vertebrate embryogenesis,presomitic mesoderm cells enter a segmental program togenerate somite,a process termed ***,a member of the bHLH transcription factor family,plays important roles in this ***,how Mespo expression is regulated remains *** this question,we isolated a genomic DNA sequence containing 4317 bp of Mespo 5’ flanking regionin *** assays show that this upstream sequence has transcription *** assayshows that this genomic contig is sufficient to recapitulate the dynamic stage-and tissue-specific expressionpattern of endogenous Mespo from the gastrula to the tailbud *** further mapped a 326 bp DNAsequence responding to retinoic acid *** results shed light on how Mespo expression is regulated,and suggest that retinoic acid signaling pathways play roles in somitogenesis through regulating Mespo.

关键词： bHLH paraxial presomitic segmental tailbud Xenopus laevis

Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells

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cell Research 2007年第3期17卷 264-270页

作者： Jihang Ju~(1,2) Ke Zou~(1,2) Hong Xie~1 ~1institute of biochemistry and cell biology,shanghai institutes for biological sciences,Chinese Academy of sciences,shanghai 200031,china ~2Graduate School of the Chinese Academy of sciences,shanghai 200031,china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of sciences Shanghai China

Hybridoma cells display an increase in antibody productivity following exposure to hypertonic ***,the underlying mechanism is not well *** the present study,we hypothesize that the nuclear factor of activatedT cells 5(NFAT5)/tonicity enhancer binding protein(TonEBP)functions to increase the antibody productivity of hybrid-oma ***5 is an osmosensitive mammalian transcription ***,its ubiquitous expression in variousorgans that are not bathed in hypertonic milieu suggests that NFAT5 may also regulate cell growth and function underisotonic *** this study,we examined the expression of NFAT5 in hybridoma cells by Western blot analysis,and found that it increased significantly in hypertonic *** further define the function of NFAT5 in hybridomacells,RNA interference technique was used to downregulate the expression of NFAT5 in SGB-8 cells(a hybridoma cellline).In isotonic medium,antibody productivity of hybridoma cells was reduced by downregulation of NFAT5 while cellproliferation was not *** results presented here demonstrate that NFAT5 not only plays an important role inosmotic stress response pathway in hybridoma cells but also is essential for optimal antibody productivity.

关键词： antibody formation hybridomas NFAT transcription factors RNA interference

EMT or apoptosis: a decision for TGF-β

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cell Research 2007年第4期17卷 289-290页

作者： Jianguo Song ~1Laboratory of Molecular cell biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,Chinese Academy of sciences,shanghai 200031,china Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Apoptosis and epithelial-to-mesenchymal transition(EMT)are very fundamental physiological *** are both independent and interrelated events in nor-mal development and in maintaining body *** recent years,EMT has emerged as a focus of studiesin cancer research,and increasing data indicate that EMTfunctions as a central step in the invasion and metastasisof some tumor *** the cytoskeleton rearrange-ment and subsequent morphological changes,EMT is oftencharacterized by the dissolution or attenuation of epithelialadhesion and other polarized structures,for example,tightjunctions,and by accordingly the acquisition of migratoryand invasive properties of the cells undergoing *** these changes,EMT can be associated with transcrip-tional or expressional alterations of some epithelial andmesenchymal *** of apoptosis and EMT are linked withvarious pathological processes,such as fibrosis of liver

关键词： a decision for TGF EMT or apoptosis

Identification of γ1 subunit of GABAA receptor in rat testis

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cell Research 2002年第1期12卷 33-37页

作者： JING HUA HU, YUAN CHANG YAN Lab of Molecular cell biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, Chinese Academy of sciences, shanghai 200031, china Lab of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

The isoform type ofγsubunits of GABAA receptor is a molecular determinant of its pharmacological characteristics. At present, the existence of GABAA receptor in mammalian sperm is still a controversy. By using degenerate primers designed according to highly conserved region in all threeγ(γ1, γ2 andγ3) subunits cloned in rat brain, we performed reverse transcription polymerase chain reaction (RT-PCR) to examine the expression pattern of -γsubunits of GABAA receptor in rat testis. Only one 370 bp fragment was obtained from RT-PCR in rat testis and sequencing results showed that it representedγ1 subunit, but notγ2 orγ3 subunit. Using the cloned fragment as probe, a 3.8 kb transcript which in size as same asγ1 ubunit in rat brain was detected in rat testis mRNA by performing Northern blot assay. Furthermore, results of in situ hybridization assay confirmed thatγ1 subunit was expressed in round spermatids and spermatozoa, maybe also in secondary spermatocyte. These evidences proved that 71 subunit of GABAA receptor is exclusively expressed in rat testis and this feature may be the structural basis of the specific function of GABAA receptors in sperm acrosome reaction.

关键词： GABAA receptor rat testis γ1 subunit.

Directional migration of leukocytes: their pathological roles in inflammation and strategies for development of anti-inflammatory therapies

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cell Research 2001年第2期11卷 85-88页

作者： GENG JIAN Guo institute of biochemistry and cell biology, shanghai institutes for biological sciences, Chinese Academy of sciences, 320 Yue Yang Road, shanghai 200031, china institute of biochemistry and cell biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Directional migration of leukocytes is indispensable to innate immunity for host defense. However, recruitment of leukocytes to a site of tissue injury the constitutes a leading cause for inflammatory responses. Mechanistically, it involves a cascade of cellular events precisely regulated by temporal and spatial present tation of a repertoire of molecules in the migrating leukocytes and their surroundings (microenvironments). Here I will summarize the emerging evidence that has shed lights on the underlying molecular mechanism for directional migration of leukocytes, which has guided the therapeutical development for innovative anti-inflammatory medicines.

关键词： Directional cell migration leukocytes inflammation therapy.

High IFN-α expression is associated with the induction of experimental autoimmune uveitis (EAU) in Fischer 344 rat

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cell Research 2001年第4期11卷 293-300页

作者： HU YONG JUN, LEI ZANG, YA DI WU, bing sun Laboratory of Molecular Immunology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, Chinese Academy of sciences, shanghai 200031, china Laboratory of Molecular Immunology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

Th1-response plays a crucial role in determining pathogenesis of organ-specific autoimmune diseases. It is believed that both IL-12 and INF-a are initiators to regulate Th1-response. In our experimental autoimmune uveitis (EAU) model, both Lewis and Fischer 344 rats share the same MHC class II molecules, while Lewis rat is EAU susceptible and Fischer 344 rat is EAU resistant. However, under the same condition of immunization, if pertussis toxin (PTX) was injected intraperitoneally as an additional adjuvant, Fischer 344 rat can develop EAU. In this study we investigate which mechanisms are involved in the induction of EAU in CFA + R16 + PTX treated (CRP- treated) Fischer 344 rats. In vivo and in vitro data demonstrated that Th1-cytokine, IFN- mRNA expression was significantly increased in disease target tissue-eyes and in draining lymph node cells of CRP-treated Fischer 344 rat. When IL-12 and IFN-a mRNA expression were compared in the experimental groups, only IFN-a mRNA expression was associated with EAU development. To distinguish the sources of IFN-a producing cells, it was observed that IFN-a expression was mainly produced by macrophages. It was further confirmed that normal macrophage from Fischer 344 rat was able to produce significant IFN-a in the presence of PTX. The data strongly suggested that IFN-a might be involved in initiating Th1-cell differentiation and in turn contribute to the induction of EAU. High IFN-a expression induced by PTX may represent a novel pathway to initiate Th1 response in Fischer 344 rat.

关键词： Experimental autoimmune uveitis(EAU) fischer 344 rat IFN-α pertussis toxin(PTX).

Regulation of mammalian pre-mRNA splicing

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Science china(Life sciences) 2009年第3期52卷 253-260页

作者： HUI JingYi State Key Laboratory of Molecular biology,institute of biochemistry and cell biology,shanghai institutes for biological sciences,Chinese Academy of sciences,shanghai 200031,china State Key Laboratory of Molecular biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

In eukaryotes,most protein-coding genes contain introns which are removed by precursor messenger RNA(pre-mRNA) *** splicing is a process by which multiple messenger RNAs(mRNAs) are generated from a single pre-mRNA,resulting in functionally distinct *** genome-wide analyses of alternative splicing indicated that in higher eukaryotes alternative splicing is an important mechanism that generates proteomic complexity and regulates gene ***-regulation of splicing causes a wide range of human *** review describes the current understanding of pre-mRNA splicing and the mechanisms that regulate mammalian pre-mRNA *** also discusses emerging directions in the field of alternative splicing.

关键词： pre-mRNA splicing alternative splicing splicing regulation signal transduction microRNA

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Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication

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cell Research 2004年第3期14卷 227-233页

作者： Yan REN, Jia Rui WU Laboratory of Molecular cell biology, institute of biochemistry and cell biology, shanghai institutes for biological sciences, Chinese Academy of sciences, 320 Yueyang Road, shanghai 200031, china. Laboratory of Molecular cell biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China

DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chkl checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern II was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly dete

关键词： intra-S-phase checkpoint DNA replication DNA damage tripchlorolide.