Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells
Downregulation of NFAT5 by RNA interference reduces monoclonal antibody productivity of hybridoma cells作者机构：Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate School of the Chinese Academy of Sciences Shanghai China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：2007年第17卷第3期
页 面：264-270页
核心收录：
学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：国家973计划
主 题：antibody formation hybridomas NFAT transcription factors RNA interference
摘 要：Hybridoma cells display an increase in antibody productivity following exposure to hypertonic ***,the underlying mechanism is not well *** the present study,we hypothesize that the nuclear factor of activatedT cells 5(NFAT5)/tonicity enhancer binding protein(TonEBP)functions to increase the antibody productivity of hybrid-oma ***5 is an osmosensitive mammalian transcription ***,its ubiquitous expression in variousorgans that are not bathed in hypertonic milieu suggests that NFAT5 may also regulate cell growth and function underisotonic *** this study,we examined the expression of NFAT5 in hybridoma cells by Western blot analysis,and found that it increased significantly in hypertonic *** further define the function of NFAT5 in hybridomacells,RNA interference technique was used to downregulate the expression of NFAT5 in SGB-8 cells(a hybridoma cellline).In isotonic medium,antibody productivity of hybridoma cells was reduced by downregulation of NFAT5 while cellproliferation was not *** results presented here demonstrate that NFAT5 not only plays an important role inosmotic stress response pathway in hybridoma cells but also is essential for optimal antibody productivity.
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