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In vitro antioxidant,antilipoxygenase and antimicrobial activities of extracts from seven climbing plants belonging to the Bignoniaceae

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Journal of Integrative Medicine 2018年第4期16卷 255-262页

作者： Carola analla torres Cristina Marisel Perez Zamora Maria Beatriz Nunez ana Maria Gonzalez laboratory of Microbiology and Pharmaceutical technology Department of Basic and Applied SciencesNational University of Chaco AustralPresidencia Roque Saenz Pena 3700ChacoArgentina National Council for scientific and technical Research(CONICEt) Godoy Cruz 2290Buenos Aires C1425FQBArgentina Institute of Botany of the Northeast(IBONE-CONICEt) Sargento Juan Bautista Cabral 2131CorrientesArgentina

Objectives： this study aimed to evaluate the in vitro antioxidant capacity, to determine the anti-inflammatory effect due to lipoxygenase inhibition and to test the antimicrobial activity of ethanolic extracts from leaves of seven climbing species belonging to the Bignoniaceae family. these species are Adenocalyrnrna rnarginatum （Cham.） DC., Amphilophium vauthieri DC., Cuspidaria convoluta （VEIl） A. H. Gentry, Dolichandra dentata （K. schum.） l. G. lohmann, Fridericia caudigera （s. Moore） l. G. lohmann, Fridericia chica （Bonpl.） l. G. lohmann and tanaecium selloi （spreng.） l. G. lohmann. Methods： the antioxidant activity was evaluated using three methods, 2,2＇-azino-bis（3-ethylbenzothiazo line-6-sulfonic acid）（ABts）, 2,2-diphenyl-l-picrylhydrazyl （DPPH）, and ferric reducing antioxidant power. lipoxygenase-inhibiting activity was assayed spectrophotometrically; the result was expressed as percent inhibition. the antimicrobial activity was assessed using the agar disk diffusion method. Minimal inhibitory concentration （MIC） and minimal bactericidal]fungicidal concentration were also determined for each extract against 12 pathogenic bacterial strains of staphylococcus aureus and seven fungal strains of the Candida genus. the identification of the major compounds present in the most promising extract was established by high-performance liquid chromatography-tandem mass spectrometry. Results： C convoluta, F. caudigera, and F. chica exhibited the best antioxidant activity by scavenging DPPH and ABts~ radicals and reducing Fe3＋ ion. these extracts showed a notable inhibition of lipnxygenase. F. caudigera was found to have the lower MIC value against s. aureus strains and six Candida species. the extracts of F. caudigera and C. convoluta were active even against methicillin-resistant s. aureus. C convoluta had higher total phenol content, better antioxidant activity and superior anti- inflammatory and antimicrobial activity. the main phenolic compounds found in this extract

关键词： Cuspidaria Fridericia Antioxidants lipoxygenase Antimicrobials Herbal drugs Free radical scavengers

Evaluation of ligninolytic activity in spent mushroom substrate from four cultivated mushrooms

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Journal of Bioresources and Bioproducts 2022年第4期7卷 288-294页

作者： ana Marión Pérez-Chávez María Melisa Alberti Edgardo Albertó laboratory of Mycology and Mushroom Cultivation.Instituto tecnológico de Chascomús(INtECH) Universidad Nacional de San Martín(UNSAM)-Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Intendente Marino AveCC 164.7130ChascomúsBuenos AiresArgentina

spent mushroom substrate(sMs)is an abundant residue generated by the mushroom industry,which is mostly *** this study,the sMs of four cultivated mushrooms were evaluated as potential sources of ligninolytic *** ostreatus,***,*** and Ganoderma lucidum were cultivated in two different substrates to determine growth,mushroom yields and ligninolytic activity of laccase(lac)and manganese peroxidase(MnP).Maximum activities in the sMs were 3.731 U/g lac and 0.191 U/g MnP,both for *** mushroom substrates were also analyzed,and higher activities were found in almost all samples.A correlation test between mushroom yields and ligninolytic activities of each culture was made,and we found that high enzymatic activity in the substrate did not result in high mushroom productivity or vice *** sMs was a good source of ligninolytic *** activity depended on the mushroom species and its affinity to the substrate,and it was not influenced by the mushroom *** of mushrooms in diverse lignocellulosic substrates is gaining interest,and ob-taining ligninolytic enzymes from residual sMs is an excellent alternative in the circular economy concept.

关键词： laccase Manganese peroxidase Biological efficiency Pleurotus spp Ganoderma lucidum Circular economy

A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root

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Molecular Plant 2021年第12期14卷 1985-1999页

作者： Moritz Graeff surbhi Rana Jos R.Wendrich Julien Dorier thomas Eekhout ana Cecilia Aliaga Fandino Nicolas Guex George W.Bassel Bert De Rybel Christian s.Hardtke Department of Plant Molecular Biology University of LausanneBiophore Building1015 LausanneSwitzerland Ghent University Department of Plant Biotechnology and BioinformaticsTechnologiepark 719000 GhentBelgium VIB Center for Plant systems Biology Technologiepark 719000 GhentBelgium Bioinformatics Competence Center University of LausanneGenopode Building1015 LausanneSwitzerland school of life sciences The University of WarwickCoventryCV47ALUK lead contact

the effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but a simple consistent positive or negative impact on a basic cellular parameter was not *** this study,we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to charac-terize root meristems and mature root segments of brassinosteroid-blind mutants and wild *** resul-tant datasets demonstrate that brassinosteroid signaling affects neither cell volume nor cell proliferation ***,brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell ***,we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions,which are sufficient to restore growth ***,single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.

关键词： Arabidopsis root meristem brassinosteroid single-cell mRNA sequencing segmentation morphology BRI1 digital single-cell analysis

single-cell RNA sequencing facilitates the elucidation of the complete biosynthesis of the antidepressant hyperforin in st. John's wort

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Molecular Plant 2024年第9期17卷 1439-1457页

作者： song Wu ana luisa Malaco Morotti Jun Yang Ertao Wang Evangelos C.tatsis National Key laboratory of Plant Molecular Genetics CASs Center for Excellence in Molecular Plant SciencesShanghai lnstitute of Plant Physiology and EcologyChineseAcademy of SciencesShanghai 200032China University of Chinese Academy of sciences Shanghai 200032China UEPAMs-CAs-JICs Centre of Excellencefor Plant anamlcroblalsclence Snangnal 200032Cnina

Hyperforin is the compound responsible for the effectiveness of ***'s wort(Hypericum perforatum)as an antidepressant,but its complete biosynthetic pathway remains *** discovery based on co-expression analysis of bulk RNA-sequencing data or genome mining failed to discover the missing steps in hyperforin *** this study,we sequenced the 1.54-Gb tetraploid *** genome assem-bled into 32 chromosomes with the scaffold N50 value of 42.44 *** single-cell RNA sequencing,we iden-tified a type of cell,“Hyper cells”,wherein hyperforin biosynthesis de novo takes place in both the leaves and *** pathway reconstitution in yeast and tobacco,we identified and characterized four transmembrane prenyltransferases(HpPt1-4)that are localized at the plastid envelope and complete the hyperforin biosynthetic *** hyperforin polycyclic scaffold is created by a reaction cascade involving an irregular isoprenoid coupling and a tandem *** findings reveal how and where hy-perforin is biosynthesized,enabling synthetic-biology reconstitution of the complete ***,this study not only deepens our comprehension of specialized metabolism at the cellularlevel but also provides strategic guidance for elucidation of the biosynthetic pathways of other specializied metabolites in plants.

关键词： hyperforin Hypericum perforatum de novo genome sequencing single-cell RNA sequencing biosyn-thetic pathway meroterpenoids

Differentiation of bee pollen samples according to the apiary of origin and harvesting period based on their amino acid content

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Food Bioscience 2022年第6期50卷 884-891页

作者： ana M.Ares laura toribio Jesús A.tapia Amelia V.González-Porto Mariano Higes Raquel Martín-Hernández JoséBernal I.U.CINQUIMA Analytical Chemistry Group (TESEA)Faculty of SciencesUniversity of ValladolidValladolidSpain Department of statistics and Operations Research Faculty of SciencesUniversity of ValladolidValladolidSpain Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de Castilla la Mancha (IRIAF) Centro de Investigación Apícola y Agroambiental (CIAPA) de MarchamaloCamino de San Martíns/n19180 MarchamaloGuadalajaraSpain Instituto de Recursos Humanos para la Ciencia y la tecnología (INCRECYt-FEDER) Fundación Parque Científico y Tecnológico de Castilla – La ManchaGuadalajaraSpain

Bee pollen is currently one of the most widely consumed dietary supplements due to its high nutritional value and its potentially beneficial effects on health. Unfortunately, in recent years an increase in the fraudulent marketing of this product has been detected, mainly in terms of adulteration with pollen from other sources. this has made it necessary to seek new tools to ensure its authentication. therefore, this study investigates the use of free amino acids as markers of the geographical origin and harvesting period of bee pollen. to demonstrate their potential as biomarkers, 72 samples from four apiaries (Pistacho, tío Natalio, Monte and Fuentelahiguera), located in the same geographical area (Marchamalo, Guadalajara, spain), were analyzed by liquid chromatography-fluorescence detection, with the data obtained undergoing canonical discriminant analysis. Variable amounts and numbers of free amino acids were found in the samples analyzed;proline predominated in all of them, in a concentration range of 298–569989 mg/kg. the differences observed in amino acid composition could be attributed to the flowering plants from which the bee pollen samples originated. In addition, it was possible to statistically assign over 75% of the samples to the corresponding apiary of origin, the best results being obtained for the Fuentelahiguera and tío Natalio apiaries (100%);this classification was even superior in the case of the harvesting periods, as more than 90% of the samples were correctly assigned, and in one period (June) a 100% rate was obtained.

关键词： Bee pollen Biomarkers Canonical discriminant analysis Food authenticity Free amino acids HPlC-FlD

Revealing the kinetics of leishmania chagasi infection in the male genital system of hamsters

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Infectious Diseases of Poverty 2016年第1期5卷 261-265页

作者： Amanda P.N.Quintal Bruna C.Borges Paula C.Brígido Rebecca t.silva ana F.Notário Marlus A.santos Maria A.de souza Fernanda G.O.Nascimento Antônio V.Mundim Guilherme M.J.Costa André B.Vasconcelos Claudio V.da silva Instituto de Ciências Biomédicas Universidade Federal de UberlândiaUberlândiaMinas GeraisBrasil Hospital Veterinário Universidade Federal de UberlândiaUberlândiaMinas GeraisBrasil Departamento de Ciências Biomédicas Universidade Federal de Minas GeraisBelo HorizonteBrasil Universidade de Uberaba UberabaMinas GeraisBrasil Departamento de Imunologia–Instituto de Ciências Biomédicas Universidade Federal de UberlândiaCampus UmuaramaUberlândiaMGBrasil

Background:leishmaniasis causes alterations and lesions in the genital system,which leads to azoospermia and testicular atrophy in animals during the chronic phase of the *** aim of this study was to reveal the kinetics of leishmania chagasi infection in the genital system of male golden hamsters(Mesocricetus auratus).Methods:Animals were intraperitoneally inoculated with amastigotes from *** different time points animals were euthanized and genital organs processed for histo-pathological,qPCR,cytokines and testosterone detection ***:Our results showed a high parasite load in testis,followed by an increase of pro-inflammatory cytokines Il1-β,tNF-αand IFN-γ,and ***,Il-4 expression was upregulated and basal parasite persistence in testis was observed using the experimental ***:Extracellular amastigotes migrated to the epididymis posing as a potential major factor of parasite persistence and venereal transmission of *** infection in hamsters.

关键词： leishmania chagasi Extracellular amastigotes testis Epididymis Immune response testosterone

Application of Bioinspired superhydrophobic surfaces in two-phase Heat transfer Experiments

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Journal of Bionic Engineering 2017年第3期14卷 506-519页

作者： Emanuele teodori ana sofia Moita Miguel Moura Pedro Pontes Antonio Moreira Yuan Bai Xinlin li Yan liu IN+- Center for Innovation Technology and Policy Research Instituto Superior T~enico Universidade de Lisboa Av. Rovisco Pais 1049-001 Lisboa Portuga Key laboratory of Bionic Engineering (Ministry of Education) Jilin University Changchun 130022 China

this paper addresses the potential to use lotus leafbioinspired surfaces in applications involving heat transfer with phase change, namely pool boiling and spray impingement. Besides describing the role of bioinspired topographical features, using an innovative technique combining high-speed visualization and time-resolved infrared thermography, surface durability is also addressed. Water is used for pool boiling and for spray impingement systems （simplified as single droplet impact）, while HFE7000 is used in a pool boiling cooler for electronic components. Results show that surface durability is quickly compro- mised for water pool boiling applications, as the chemical treatment does not withstand high temperatures （t 〉 100 ℃） during long time intervals （3 h - 4 h）. For HFE7000 pool boiling （depicting lower saturation temperature - 34 ℃）, heat transfer en- hancement is governed by the topography. the regular hierarchical pattern of the bioinspired surfaces promotes the heat transfer coefficient to increase up to 22.2%, when compared to smooth surfaces, while allowing good control of the interaction mechanisms until a distance between micro-structures of 300 gm- 400 Ixm. Droplet impingement was studied for surface temperatures ranging between 60 ℃ - 100 ℃. the results do not support the use of superhydrophobic surfaces for cooling applications, but reveal great potential for other applications involving droplet impact on heated surfaces （e.g. metallurgy in- dustry）.

关键词： bioinspired surfaces surface micro-patterning two-phase heat transfer time resolved infrared thermography

Dynamical evolution of Ge quantum dots on si(111):From island formation to high temperature decay

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Aggregate 2022年第4期3卷 114-120页

作者： Navathej Preetha Genesh Fabrizio De Marchi stefan Heun stefano Fontana Rachid Belkhou Rahul Purandare Nunzio motta Anna sgarlata Massimo Fanfoni Jennifer Macleod Oliver Maclean Federico Rosei CentreÉnergie Matériaux et TélécommunicationsInstitut National de la Recherche ScientifiqueQuébecCanada NEst Istituto Nanoscienze-CNR and Scuola Normale SuperiorePisaItaly sincrotrone trieste sCpA BasovizzaItaly lURE UniversitéParis-SudOrsay CedexFrance school of Chemistry and Physics and Centre for Materials science Queensland University of TechnologyBrisbaneAustralia Dipartimento di Fisica Universitàdi Roma“Tor Vergata”RomeItaly Key laboratory of Functional Materials Physics and Chemistry of the Ministry of Education Jilin Normal UniversityChangchunP.R.China

Heteroepitaxial growth is a process of profound fundamental importance as well as an avenue to realize nanostructures such as Ge/si quantum dots(QDs),with appealing properties for applications in opto-and ***,controlling the Ge/si QD size,shape,and composition remains a major obstacle to their practical ***,Ge nanostructures on si(111)were investigated in situ and in real-time by low energy electron microscopy(lEEM),enabling the observation of the transition from wetting layer formation to 3D island growth and *** island size,shape,and distribution depend strongly on the growth *** the deposition temperature increases,the islands become larger and sparser,consistent with Brownian nucleation and capture *** 550◦C,two distinct Ge/si nanostructures are formed with bright and dark appearances that correspond to flat,atoll-like and tall,faceted islands,*** annealing,the faceted islands increase in size at the expense of the flat ones,indicating that the faceted islands are thermodynamically more *** contrast,triangular islands with uniform morphology are obtained from deposition at 600◦C,suggesting that the growth more closely follows the ideal *** annealing,the islands formed at 600◦C initially show no change in morphology and size and then rupture simultaneously,signaling a homogeneous chemical potential of the *** observations reveal the role of dynamics and energetics in the evolution of Ge/si QDs,which can serve as a step towards the precise control over the Ge nanostructure size,shape,composition,and distribution on si(111).

关键词： epitaxial growth Gesi heterostructures low energy electron microscopy

Bioguided isolation of antimicrobial polyphenols from Cuspidaria convoluta leaves and their synergistic effect with antibiotics

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Asian Pacific Journal of tropical Biomedicine 2019年第10期9卷 434-442页

作者： Carola A.torres Mario A.sturla ana M.Romero María A.Judis Department of Basic and Applied sciences Microbiology LaboratoryUniversidad Nacional del Chaco AustralComandante Fernández 755Presidencia Roque Sáenz PenaChacoArgentina Consejo Nacional de Investigaciones Científicas y técnicas Buenos AiresArgentina Department of Basic and Applied sciences Laboratory of Organic ChemistryUniversidad Nacional del Chaco AustralArgentina Department of Basic and Applied sciences Food Industry LaboratoryUniversidad Nacional del Chaco AustralArgentina

Objective:to identify and isolate phenolic compounds from Cuspidaria convoluta,and to evaluate their antibacterial activity and synergistic effect with ***:the crude extract was prepared by maceration with methanol(5%).the dry extract was suspended in water and fractionated *** most active extract was selected by its antibacterial activity and its total phenol content was determined by spectrophotometry and by HPlC-Ms/*** fractions of the most active extract were separated by column chromatography and evaluated by *** compounds were *** inhibitory concentration(MIC)of these compounds was determined by microdilution broth method,and synergism with antibiotics(ampicillin,gentamicin and oxacillin)was tested by checkerboard and time-kill ***:Coumaric acid,catechin/epicatechin,and luteolin were purified and identified from the *** was an increase in the antibacterial activity of antibiotics when they were combined with these *** combination of luteolin and ampicillin had the most potent antibacterial *** MICs of oxacillin for each of methicillin-resistant staphylococcus aureus strains were reduced between 4 and 8-fold when these strains were coincubated with sub-MIC(≤?MIC)levels of these compounds,demonstrating that the combination had synergistic effect for all ***:Cuspidaria convoluta contains important pharmacologically active substances that can be used to improve antibiotic efficacy.

关键词： luteolin Ampicillin MIC reduction Antimicrobial agents