A single-cell morpho-transcriptomic map of brassinosteroid action in the Arabidopsis root

作     者：Moritz Graeff Surbhi Rana Jos R.Wendrich Julien Dorier Thomas Eekhout Ana Cecilia Aliaga Fandino Nicolas Guex George W.Bassel Bert De Rybel Christian S.Hardtke Moritz Graeff;Surbhi Rana;Jos R.Wendrich;Julien Dorier;Thomas Eekhout;Ana Cecilia Aliaga Fandino;Nicolas Guex;George W.Bassel;Bert De Rybel;Christian S.Hardtke

作者机构：Department of Plant Molecular BiologyUniversity of LausanneBiophore Building1015 LausanneSwitzerland Ghent UniversityDepartment of Plant Biotechnology and BioinformaticsTechnologiepark 719000 GhentBelgium VIB Center for Plant Systems BiologyTechnologiepark 719000 GhentBelgium Bioinformatics Competence CenterUniversity of LausanneGenopode Building1015 LausanneSwitzerland School of Life SciencesThe University of WarwickCoventryCV47ALUK Lead contact 

出 版 物：《Molecular Plant》 (分子植物（英文版）)

年 卷 期：2021年第14卷第12期

页      面：1985-1999页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：funded by core funding from the University of Lausanne,the Swiss National Science Foundation(grant 310030B_185379,awarded to C.S.H.) The Research Foundation-Flanders(FWO,post-doc fellowship 1215820N,awarded to T.E.) the European Research Council(ERC Start ing Grant TORPEDO,714055,awarded to B.D.R.) the BBSRC(grant BB/S002804/1 to G.W.B.) the Deutsche Forschungsgemeinschaft(DFG,post-doctoral fellowship GR 5009/1-1,awarded to M.G.) 

主　　题：Arabidopsis root meristem brassinosteroid single-cell mRNA sequencing segmentation morphology BRI1 digital single-cell analysis 

摘      要：The effects of brassinosteroid signaling on shoot and root development have been characterized in great detail but a simple consistent positive or negative impact on a basic cellular parameter was not *** this study,we combined digital 3D single-cell shape analysis and single-cell mRNA sequencing to charac-terize root meristems and mature root segments of brassinosteroid-blind mutants and wild *** resul-tant datasets demonstrate that brassinosteroid signaling affects neither cell volume nor cell proliferation ***,brassinosteroid signaling is essential for the precise orientation of cell division planes and the extent and timing of anisotropic cell ***,we found that the cell-aligning effects of brassinosteroid signaling can propagate to normalize the anatomy of both adjacent and distant brassinosteroid-blind cells through non-cell-autonomous functions,which are sufficient to restore growth ***,single-cell transcriptome data discern directly brassinosteroid-responsive genes from genes that can react non-cell-autonomously and highlight arabinogalactans as sentinels of brassinosteroid-dependent anisotropic cell expansion.