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Early Detection of White Spot Syndrome Virus(WSSV)in Isolated Hemocytes of Litopenaeus vannamei

CellBio 2017年第1期6卷 1-12页

作者： Ariadne Hernandez-Perez Rossanna Rodriguez-Canul Edgar Torres-Irineo Fernando mendoza-Cano Daniel Eduardo Coronado-Molina Jesus alejandro Zamora-Briseno Jorge Hernandez-Lopez Laboratorio de Inmunología y Biología Molecular Centro de Investigación y de Estudios Avanzados del IPN(CINVESTAV-IPN)Unidad MéridaMéridaMéxico Laboratorio de Pesquerías Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-Unidad MéridaMéridaMéxico UMDI-Sisal Facultad de CienciasUniversidad Nacional Autónoma de MéxicoSierra PapacalMéxico Centro de Investigaciones Biológicas del Noroeste HermosilloMéxico

To date, White Spot Syndrome (WSS) produced by the White Spot Syndrome Virus (WSSV) causes one of the most severe diseases infecting penaeid shrimps worldwide. Although a vast amount of studies has elucidated pathogenesis in live infection models, there is still little information about the interaction of WSSV infections using in vitro models in the whiteleg shrimp Litopenaeus vannamei (L. vannamei) hemocytes. In this study, a WSSV infection kinetics was performed using total hemocytes isolated from healthy L. vannamei organisms and maintained in in vitro conditions using isotonic solution for shrimp (ISS). The infected experimental cells received ≈ 30,000 viral copies of WSSV. The viability of the hemocytes (control and infected group) was measured during the kinetics with trypan blue exclusion method and cells were maintained up to 6 hpi (post-infection) with non-significant differences of viability between both groups. WSSV replication was assessed using RT- PCR at the RNA expression level of the early viral gene Ie1 and transcripts were detected as early as 30 min pi. Hemocytes from WSSV group showed disrupted integrity, degranulation and irregular shape. This study provides evidence of the capability of WSSV to infect and replicates in L. vannamei hemocytes using in vitro assays in short times as 30 min.

关键词： WSSV Litopenaeus vannamei Ie1 gene Hemocytes In Vitro Infection gLS Analysis

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Effect of the sintering technique on the ferroelectric and d33 piezoelectric coefficients of Bi0.5(Na0.84K0.16)0.5TiO3 ceramic

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Journal of Advanced Ceramics 2019年第2期8卷 278-288页

作者： g.HERNANDEZ-CUEVAS J.R.LEYVA mendoza P.E.gARCíA-CASILLAS C.A.RODRígUEZ gONZáLEZ J.F.HERNANDEZ-PAZ g.HERRERA-PéREZ L.FUENTES-COBAS S.DíAZ DE LA TORRE O.RAYMOND-HERRERA H.CAMACHO-MONTES Instituto de Ingeniería y Tecnología Universidad Autónoma de Ciudad JuárezAv.Del Charro 450 Norte Cd.JuárezChihuahua32310México Centro de Investigación en Materiales Avanzados Miguel de Cervantes 120Chihuahua 31109ChihuahuaMéxico Centro de Investigación e Innovación Tecnológica Instituto Politécnico NacionalCerrada de Cecati s/nAzcapotzalcoSanta Catarina02250Ciudad de MéxicoMéxico Centro de Nanociencias y Nanotecnología Universidad Nacional Autónoma de MéxicoKm.107 Carretera Tijuana-EnsenadaAP.14Ensenada 22860Baja CaliforniaMéxico

In the search of lead-free piezoelectric materials,ceramic processing techniques offer potential tools to increase the piezoelectric and ferroelectric properties in addition to new chemical *** of pure BNKT16(Bi0.5(Na0.84K0.16)0.5TiO3)phase were synthesized by sol–gel method with a low crystallization temperature(750℃).Ceramic samples were sintered by pressureless sintering(PLS),sinter-forging(SF),and spark plasma sintering(SPS)***,morphological,and chemical characterizations were performed by XRD,Raman,EDS,and *** samples by PLS and SF exhibit rod-like grains associated to bismuth *** highest remanent polarization(11.05μC/cm2),coercive field(26.2 kV/mm),and piezoelectric coefficient(165 pC/N)were obtained for SF *** piezoresponse force microscopy(PFM)analysis shows that the crystallites at the nanoscale exhibit piezoelectric phenomenon and the highest piezoelectric response is reported for PLS *** presence of the rhombohedral phase,the increase in grain and crystallite size,and the oriented rod-like inclusions favoring the crystallographic texture are facts that enhance the piezoelectric coefficient for BNKT16 piezoceramics.

关键词： lead-free piezoelectrics sol–gel pressureless sintering (PLS) sinter-forging (SF) spark plasma sintering(SPS) piezoresponse

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Porous Carbon grown by Chemical Vapor Deposition on Copper Substrates

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Journal of Materials Science and Chemical Engineering 2015年第8期3卷 16-20页

作者： D. mendoza Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México México D. F. México

Amorphous porous carbon was synthesized by chemical vapor deposition on copper substrates. The average size of the pores is around 1.2 microns with some small pores decorating the big ones. Lamellar samples of this carbonaceous material can be separated from the copper support and may be useful as electrode due to its low electrical resistivity of the order of 0.4 Ωcm.

关键词： Porous Carbon graphene-Like Materials Conductive Electrodes Chemical Vapor Deposition

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Multi-objective parametrization of interatomic potentials for large deformation pathways and fracture of two-dimensional materials

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npj Computational Materials 2021年第1期7卷 1042-1052页

作者： Xu Zhang Hoang Nguyen Jeffrey T.Paci Subramanian K.R.S.Sankaranarayanan Jose L.mendoza-Cortes Horacio D.Espinosa Theoretical and Applied Mechanics Program Northwestern UniversityEvanstonILUSA Department of Chemistry University of VictoriaVictoriaBCCanada Center for Nanoscale Materials Argonne National LaboratoryLemontILUSA Department of Chemical Engineering&Materials Science Michigan State UniversityEast LansingMIUSA Department of Mechanical Engineering Northwestern UniversityEvanstonILUSA

This investigation presents a generally applicable framework for parameterizing interatomic potentials to accurately capture large deformation *** incorporates a multi-objective genetic algorithm,training and screening property sets,and correlation and principal component *** framework enables iterative definition of properties in the training and screening sets,guided by correlation relationships between properties,aiming to achieve optimal parametrizations for properties of ***,the performance of increasingly complex potentials,Buckingham,Stillinger-Weber,Tersoff,and modified reactive empirical bond-order potentials are *** MoSe_(2)as a case study,we demonstrate good reproducibility of training/screening properties and superior *** MoSe_(2),the best performance is achieved using the Tersoff potential,which is ascribed to its apparent higher flexibility embedded in its functional *** results should facilitate the selection and parametrization of interatomic potentials for exploring mechanical and phononic properties of a large library of two-dimensional and bulk materials.

关键词： materials apparent enable

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Ni_xWO_(2.72) nanorods as an efficient electrocatalyst for oxygen evolution reaction

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green Energy & Environment 2017年第2期2卷 119-123页

作者： Zheng Xi Adriana mendoza-garcia Huiyuan Zhu MiaoFang Chi Dong Su Daniel P.Erdosy Junrui Li Shouheng Sun Department of Chemistry Brown University Chemical Sciences Division Oak Ridge National Laboratory Center for Nanophase Materials Sciences Division Oak Ridge National Laboratory Center for Functional Nanomaterials Brookhaven National Laboratory

Ni_xWO_(2.72) nanorods(NRs) are synthesized by a one-pot reaction of Ni(acac)_2 and WCl_4. In the rod structure, Ni(Ⅱ) intercalates in the defective perovskite-type WO_(2.72) and is stabilized. The Ni_xWO_(2.72) NRs show the x-dependent electrocatalysis for the oxygen evolution reaction(OER) in 0.1 M KOH with Ni_(0.78)WO_(2.72) being the most efficient, even outperforming the commercial Ir-catalyst. The synthesis is not limited to Ni_xWO_(2.72) but can be extended to M_xWO_(2.72)(M = Co, Fe) as well,providing a new class of oxide-based catalysts for efficient OER and other energy conversion reactions.

关键词： Tungsten oxide 3d transition metal doping Nanorods Oxygen evolution reaction Electrocatalysis

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Empirical evaluation of confidence and prediction intervals for spatial models of forest structure in Jalisco,Mexico

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Journal of Forestry Research 2011年第2期22卷 159-166页

作者： Robin M.Reich C.Aguirre-Bravo Vanessa A.Bravo Martin mendoza Briseo Department of Forest and Rangeland Stewardship Colorado State UniversityFort CollinsCO 80523-1472 USA USDA Forest Service ArlingtonVA 22209-2131 USA Clegio de Postgraduados Campus MontecillosTexcocoMexico

In recent years there has been an increasing interest in developing spatial statistical models for data sets that are seemingly spatially *** lack of spatial structure makes it difficult,if not impossible to use optimal predictors such as ordinary kriging for modeling the spatial variability in the *** many instances,the data still contain a wealth of information that could be used to gain flexibility and precision in *** this paper we propose using a combination of regression analysis to describe the large-scale spatial variability in a set of survey data and a tree-based stratification design to enhance the estimation process of the small-scale spatial *** this approach,sample units（i.e.,pixel of a satellite image） are classified with respect to predictions of error attributes into homogeneous classes,and the classes are then used as strata in the stratified *** variables used as a basis of stratification included terrain data and satellite imagery.A decision rule was used to identify a tree size that minimized the error in estimating the variance of the mean response and prediction uncertainties at new spatial *** approach was applied to a set of n=937 forested plots from a state-wide inventory conducted in 2006 in the Mexican State of *** final models accounted for 62% to 82% of the variability observed in canopy closure（%）,basal area（m2·ha-1）,cubic volumes（m3·ha-1） and biomass（t·ha-1） on the sample *** spatial models provided unbiased estimates and when averaged over all sample units in the population,estimates of forest structure were very close to those obtained using classical estimates based on the sampling strategy used in the state-wide *** spatial models also provided unbiased estimates of model variances leading to confidence and prediction coverage rates close to the 0.95 nominal rate.

关键词： tree-based stratified design generalized least squares standardized mean squared error Landsat-7 ETM+

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Electrical Conductivity of Collapsed Multilayer graphene Tubes

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World Journal of Nano Science and Engineering 2012年第2期2卷 53-57页

作者： D. mendoza Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México Coyoacán México

Synthesis of multilayer graphene on copper wires by a chemical vapor deposition method is reported. After copper etching, the multilayer tube collapses forming stripes of graphitic films, their electrical conductance as a function of temperature indicate a semiconductor-like behavior. Using the multilayer graphene stripes, a cross junction is built and owing to its electrical behavior we propose that a tunneling process exists in the device.

关键词： Multilayer graphene Electrical Conductivity Klein Tunneling Chemical Vapor Deposition

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Collagen matrix scaffolds:Future perspectives for the management of chronic liver diseases

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World Journal of Clinical Cases 2023年第6期11卷 1224-1235页

作者： Moises Martinez-Castillo Itzel Altamirano-mendoza Rafal Zielinski Waldemar Priebe Cristina Piña-Barba gabriela gutierrez-Reyes Liver Pancreas and Motility LaboratoryUnit of Experimental MedicineSchool of MedicineUniversidad Nacional Autonoma de MexicoMexico City 06726Mexico CityMexico Department of Experimental Therapeutics The University of Texas MD Anderson Cancer CenterHoustonTX 77054United States Materials Research Institute Universidad Nacional Autónoma de MéxicoMexico City 06726Mexico CityMexico

Approximately 1.5 billion chronic liver disease(CLD)cases have been estimated worldwide,encompassing a wide range of liver damage ***,liver disease causes approximately 1.75 million deaths per *** is typically characterized by the silent and progressive deterioration of liver parenchyma due to an incessant inflammatory process,cell death,over deposition of extracellular matrix proteins,and dysregulated ***,these processes impair the correct function of this vital *** and liver cancer are the main complications of CLD,which accounts for 3.5%of all deaths *** transplantation is the optimal therapeutic option for advanced liver *** liver is one of the most common organs transplanted;however,only 10%of liver transplants are *** this context,regenerative medicine has made significant progress in the design of biomaterials,such as collagen matrix scaffolds,to address the limitations of organ transplantation(e.g.,low donation rates and biocompatibility).Thus,it remains crucial to continue with experimental and clinical studies to validate the use of collagen matrix scaffolds in liver disease.

关键词： Liver Chronic liver disease Collagen matrix scaffold Transplant Management

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Antenatal use of bosentan and/or sildenafil attenuates pulmonary features in rats with congenital diaphragmatic hernia

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World Journal of Pediatrics 2014年第4期10卷 354-359页

作者： María de Lourdes Lemus-Varela Amed Soliz Belinda Claudia gómez-Meda Ana Lourdes Zamora-Perez José Manuel Ornelas-Aguirre Valery Melnikov Blanca Miriam Torres-mendoza guillermo Moisés Zúñiga-gonzález Division of Neonatology High Specialty Medical UnitPediatric HospitalWestern National Medical CenterMexican Social Security InstituteGuadalajaraJaliscoMéxico Neonatal Intensive Care Unit Division of NeonatologyMiami Children's HospitalMiami FLDUSA Molecular Biology in Medicine Institute Molecular Biology and Genomic DepartmentHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Odontology Research Institute Integrated Odontology Clinic DepartmentHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Pathology Department High Specialty Medical UnitNorth-western National Medical CenterMexican Social Security InstituteCiudad ObregónSonoraMéxico School of Medicine University of ColimaColimaMéxico Division of Neurosciences Western Biomedical Research CenterMexican Social Security Institute and Department of ClinicsHealth Sciences University CenterUniversity of GuadalajaraGuadalajaraJaliscoMéxico Mutagenesis Laboratory Western Biomedical Research CenterMexican Social Security InstituteGuadalajaraJaliscoMéxico

Background:Lung hypoplasia,pulmonary persistent hypertension of the newborn and its morphological changes are the main features in congenital diaphragmatic hernia(CDH).This study was undertaken to investigate if antenatal use of sildenafil and/or bosentan attenuates vascular remodeling,promotes branching,and improves alveolarization in experimental nitrofeninduced ***:Nitrofen(100 mg)was gavage-fed to pregnant rats at post conception day(PCD)9 to induce *** rats were randomized to 5 groups:1)control;2)nitrofen;3)nitrofen+sildenafil 100 mg/kg per day at PCD 16-20;4)nitrofen+bosentan 30 mg/kg per day,at PCD 16-20,and 5)nitrofen+bosentan+sildenafil,same doses and administration *** cesarean delivery,the offsprings were sacrifi *** diaphragmatic defect and pulmonary hypoplasia were identified,and the lungs were *** wall thickness,bronchiolar density and alveolarization were ***:The offsprings with CDH were characterized by severe pulmonary hypoplasia(lung weight-to-body weight ratio:0.0263[95%confidence interval(CI)0.0242-0.0278)]in the nitrofen group versus 0.0385(95%CI 0.0355-0.0424)in the control group(P=0.0001).Pulmonary arterial wall thickness was decreased to 3.0(95%CI 2.8-3.7)μm in the nitrofen+sildenafil group versus 5.0(95%CI 4.1-4.9)μm in the nitrofen group(P=0.02).Terminal bronchioles increased to 13.7(95%CI 10.7-15.2)μm in the nitrofen+bosentan group in contrast to 8.7(95%CI 7.2-9.4)μm in the nitrofen group(P=0.002).More significant differences(P=0.0001)were seen in terminal bronchioles in the nitrofen+sildenafil+bosentan group than in the nitrofen group[14.0(95%CI 12.5-15.4)μm versus 8.5(95%CI 7.1-9.3)μm].Pulmonary arterial wall thickness was also decreased in the former ***:In this rat model,antenatal treatment with sildenafil attenuates vascular *** promotes the development of terminal bronchioles in nitrofen-induced CDH.

关键词： antenatal treatment bosentan congenital diaphragmatic hernia pulmonary hypoplasia sildenafil

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green Synthesis of Silver Nanoparticles Using Annona diversifolia Leaf Extract and Their Antimicrobial Application

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Journal of Renewable Materials 2020年第9期8卷 1129-1137页

作者： Rogelio Solorzano-Toala Daniel gonzalez-mendoza Benjamin Valdez-Salas Vianey Mendez-Trujillo Federico gutierrez-Miceli Ernesto Beltran-Partida Olivia Tzintzun-Camacho Tecnológico Nacional de México-Campus Tuxtla gutiérrez Tuxtla Gutiérrez03940México Instituto de Ciencias Agrícolas de la Universidad Autónoma de Baja California Mexicali21705México Instituto de Ingeniería de la Universidad Autónoma de Baja California Mexicali21100México Facultad de Medicina de la Universidad Autónoma de Baja California Mexicali21100México

The aim of this study was the synthesis of silver nanoparticle using Annona diversifolia *** silver nanoparticles obtained were analyzed by spectroscopic methods and dynamic light scattering *** inhibition of AgNPs was evaluated against Bacillus cereus,Klebsiella pneumoniae and Enterobacter *** results showed that AgNPs have high values at 3 keV and particle size between 45 to 58 nm with a homogenous *** AgNPs showed growth inhibition against Klebsiella pneumoniae and Enterobacter *** studies are needed to confirm the potential antimicrobial of different AgNP from *** in gram negative and gram positive bacteria.

关键词： green chemistry AgNPs plants antimicrobial biotechnology

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