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NBS1 Interacts with CyclinB to Modulate Drug Sensitivity of Olaparib in Ovarian Cancer

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NBS1 Interacts with CyclinB to Modulate Drug Sensitivity of ...

2022CCTB中国肿瘤标志物学术大会暨中国整合肿瘤学大会暨第十六届肿瘤标志物青年科学家论坛暨中国肿瘤标志物产业创新大会

作者： ailing zhong Renquan Lu Lin Guo Fudan University Shanghai Cancer Center

Dysfunction of the DNA repair pathway contributes to tumorigenesis and drug ***1 is a key molecular involved in homologous recombination(HR) repair after DNA double-strand *** plays a crucial role in DNA replication,repair and transcription,which is also important for maintaining the secondary structure of DNA;therefore,their activity must be precisely controlled to ensure genome *** previously described that NBS1expression was significantly correlated with CyclinB expression based on protein microarray *** is a cell-cycle regulatory protein that has been shown to be involved in the activation of DNA damage repair checkpoints by inducing G2/M phase arrest,promoting apoptosis,and participating in the regulation of chemotherapeutic drug sensitivity by inducing nuclear degradation,as shown by immunofluorescence *** this study,NBS1-downregulated cells were generated using lentivirus-mediated *** a qPCR-based HR assay kit to detected HR *** sensitivity of NBS1 to olaparib was investigated by cell proliferation,colony formation assays and flow *** results demonstrated that the inhibition of NBS1 expression activated the CyclinB signaling pathways and sensitized ovarian cancer to X-ray and Olaparib treatment both in vitro and in ***1 may be a potential therapeutic target for epithelial ovarian cancer(EOC).

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COPS5 inhibition arrests the proliferation and growth of serous ovarian cancer cells via the elevation of p27 level

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COPS5 inhibition arrests the proliferation and growth of ser...

2017年中国肿瘤标志物学术大会暨第十一届肿瘤标志物青年科学家论坛

作者： Hongqin Zhang ailing zhong Miaomiao Chen Renquan Lu Department of Clinical Laboratory Fudan University Shanghai Cancer Center

Background: COPS5 is a pivotal subunit of COP9 signalosome complex. It not only acts as the catalytic center of the complex but also stably exists as a monomer. Aberrant expression of COPS5 is correlated with decreased p27 expression. Ovarian cancer is the most lethal gynecological malignancies, however, the role of COPS5 in ovarian cancer remain to be clarified. Methods: The expression of COPS5 was predicted by bioinformatics analysis and confirmed by immunohistochemistry. COPS5 and COPS6 gene were downregulated by RNA interference(RNAi). The biological role of COPS5 in the transduced cells were evaluated by cell proliferation, colony formation, apoptosis and cell cycle analysis. The interactions of COPS5 and p27 were identified by immunoprecipitation and immunofluorescence. The biological effects of the specific COPS5 inhibitor CSN5 i-3 and/or MK2206 were accessed by CCK-8 and flow ***: COPS5 expression was significantly increased in serous ovarian cancer(SOC) cells and tissues compared with those controls. SOC cells with COPS5 depletion were arrested in G1/G0-phase and exhibited a reduced proliferation ability and an increased cytoplasmic p27 expression. Whereas, the cells were stuck at S-phase accompanied with an elevation of nucleus p27 expression after blocking COPS5 by CSN5 i-3 and COPS6 suppression. Furthermore, inhibition of COPS5 resulted in an elevation of Akt expression and sensitized ovarian cancer cells to MK2206. Conclusion: Inhibition of COPS5 resulted in the elevation of p27 expression, as well as the formation of a novel COPS6 containing CSN sub-complex. Meanwhile, COPS5 depletion promoted Akt expression which might be a negative feedback mechanism of p27. COPS5 knockdown enhanced the inhibitory effect of MK2206 on SOC cells proliferation. Suppression of COPS5 and Akt offers a potential strategy and facilitates the development of targeting therapy for serous ovarian cancers.

关键词： COPS5 COPS6 serous ovarian cancer p27 Akt

A combined strategy of TK1, HE4 and CA125 shows better diagnostic performance than Risk of Ovarian Malignancy Algorithm(ROMA) in ovarian carcinoma

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A combined strategy of TK1, HE4 and CA125 shows better diagn...

2021年中国肿瘤标志物学术大会暨第十五届肿瘤标志物青年科学家论坛

作者： Cheng Zhu Lin Guo Renquan Lu ailing zhong Kangjia Xiao Fudan University Shanghai Cancer Center

Background: The dual marker algorithm Risk of Ovarian Malignancy Algorithm(ROMA) has been widely used in the clinic for identification of equivocal pelvic masses in ovarian carcinoma. To obtain higher diagnostic efficiency, we created a new diagnostic index Risk of Ovarian Malignancy Index(ROMI) by combing thymidine kinase 1(TK1), HE4 and ***: 335 patients with pelvic masses on imaging and 46 healthy controls were enrolled. Serum TK1 was analyzed before further study. ROMI and ROMA were evaluated for diagnostic ***: The level of TK1 was elevated in malignant ovarian tumors compared to benign masses(P<0.001)and healthy controls(P<0.001). TK1 was positively correlated with stage, intrapelvic metastasis,lymphatic metastasis and distant metastasis(all P values <0.001). The area under the receiver operating characteristic curve(AUC) of ROMI was higher than that of ROMA for both pre-and postmenopausal women. ROMI had better sensitivity, specificity, accuracy, positive and negative predictive values than ROMA in diagnosis of all-stage or stage I+Ⅱ ovarian carcinoma for both preand postmenopausal ***: TK1 is a potential biomarker in detection of ovarian carcinoma. ROMI shows better diagnostic performance than ROMA in distinguishing malignant ovarian tumors from benign masses.

关键词： Ovarian cancer Algorithm Thymidine kinase 1 Risk of Ovarian Malignancy Index Risk of Ovarian Malignancy Algorithm Tumor markers

Identifying a confused cell identity for esophageal squamous cell carcinoma

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1692-1702页

作者： Xiangyu Pan Jian Wang Linjie Guo Feifei Na Jiajia Du Xuelan Chen ailing zhong Lei Zhao Lu Zhang Mengsha Zhang Xudong Wan Manli Wang Hongyu Liu Siqi Dai Ping Tan Jingyao Chen Yu Liu Bing Hu Chong Chen Department of Gastroenterology State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengduSichuanChina Department of Thoracic Oncology State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041 SichuanChina Dpartment of Urology Institute of UrologyWest China HospitalSichuan UniversityChengduChina

The cell identity of malignant cells and how they acquire it are fundamental for our understanding of ***,we report that esophageal squamous cell carcinoma(ESCC)cells display molecular features equally similar but distinct to all three types of normal esophageal epithelial cells,which we term as confused cell identity(CCI).CCI is an independent prognostic marker associated with poor prognosis in ***,we identify tropomyosin 4(TPM4)as a critical CCI gene that promotes the aggressiveness of ESCC in vitro and in *** TPM4 creates CCI through activating the Jak/STAT-SOX2 ***,our study suggests an unrecognized feature of ESCC cells,which might be of value for clinic prognosis and potential interference.

关键词： esophageal SOX2 squamous

缩减约束方案应用于预防重症加强监护病房气管插管非计划性拔管的可行性分析

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解放军护理杂志 2019年第5期36卷 47-50页

作者：张艳仲爱玲南通大学附属海安医院海安县人民医院外科ICU

目的探讨缩减约束方案应用于预防重症加强监护病房(intensive care unit ,ICU)气管插管非计划性拔管的效果。方法便利抽样选择2015年5月至2016年5月我院收治的行常规身体约束护理方案的ICU气管插管患者75例作为对照组,同法选择2016年6月... 详细信息

目的探讨缩减约束方案应用于预防重症加强监护病房(intensive care unit ,ICU)气管插管非计划性拔管的效果。方法便利抽样选择2015年5月至2016年5月我院收治的行常规身体约束护理方案的ICU气管插管患者75例作为对照组,同法选择2016年6月至2017年6月我院收治的行缩减约束护理方案的ICU气管插管患者75例作为观察组。采用症状自评量表(symptom check list 90,SCL-90)对两组患者清醒后心理状态进行评估,并对比两组患者的约束情况及护理满意度。结果护理干预后,观察组患者SCL-90总分及各维度评分均低于对照组,平均约束时间短于对照组,身体约束率、非计划性拔管发生率低于对照组,护理满意度优于对照组,差异均有统计学意义(均P<0.05).结论缩减约束方案应用于ICU气管插管患者可有效改善其心理状态,减少身体约束,降低非计划性拔管发生率提高护理满意度,值得临床推广应用。

关键词：缩减约束方案 ICU 气管插管心理状态非计划性拔管

用户请求感知的边端缓存与用户推荐联合优化策略

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电子与信息学报 2024年第7期46卷 2850-2859页

作者：王汝言蒋昊唐桐吴大鹏钟艾玲重庆邮电大学通信与信息工程学院重庆400065 先进网络与智能互联技术重庆市高校重点实验室重庆400065 泛在感知与互联重庆市重点实验室重庆400065

针对当前边缘缓存场景中普遍存在的用户偏好未知与高度异质问题,该文提出一种用户请求感知的边端缓存与用户推荐联合优化策略。首先,建立点击率(CTR)预测基本模型,引入对比学习方法生成高质量的特征表示,辅助因子分解机(FM)预测用户偏好... 详细信息

针对当前边缘缓存场景中普遍存在的用户偏好未知与高度异质问题,该文提出一种用户请求感知的边端缓存与用户推荐联合优化策略。首先,建立点击率(CTR)预测基本模型,引入对比学习方法生成高质量的特征表示,辅助因子分解机(FM)预测用户偏好;然后,基于用户偏好设计一种动态推荐机制,重塑不同用户的内容请求概率,从而影响缓存决策;最后,以用户平均内容获取时延最小化为目标建立边端缓存与用户推荐联合优化问题,将其解耦为边端缓存子问题和用户推荐子问题,分别基于区域贪婪算法和一对一交换匹配算法求解,并通过迭代更新获得收敛优化结果。仿真结果表明,相较于基准模型,引入对比学习方法的预测模型在曲线下面积(AUC)和准确率(ACC)上分别提升1.65%和1.30%,且联合优化算法能够有效降低用户平均内容获取时延,提升系统缓存性能。

关键词：边缘缓存对比学习推荐机制平均时延

建立一种预测中晚期鼻咽癌患者复发转移的模型

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中华检验医学杂志 2023年第10期46卷 1059-1066页

作者：李想仲瑷玲卢仁泉郭林复旦大学附属肿瘤医院检验科复旦大学上海医学院肿瘤系上海200032

目的建立中晚期鼻咽癌患者复发转移风险评估模型。方法收集2012年3月1日至2020年8月31日在复旦大学附属肿瘤医院进行首次治疗的中晚期鼻咽癌患者242例, 年龄(48.33±11.13)岁, 男性178例, 女性64例, 生存期(3.39±1.42)年。根据随机数... 详细信息

目的建立中晚期鼻咽癌患者复发转移风险评估模型。方法收集2012年3月1日至2020年8月31日在复旦大学附属肿瘤医院进行首次治疗的中晚期鼻咽癌患者242例, 年龄(48.33±11.13)岁, 男性178例, 女性64例, 生存期(3.39±1.42)年。根据随机数字表法将入组对象分成2组, 其中建模组192例, 验证组50例。采集患者治疗前、首次治疗后以及治疗后随访期的静脉血, 分析其血细胞分类、血生化指标, 利用t检验与χ2检验, 以是否发生复发转移为研究结局, 分析指标在患者预后中的差异。采用多因素Cox回归分析, 筛选出影响鼻咽癌患者复发转移的独立预后因素, 构建患者2、4、6年复发转移风险Nomogram模型, 计算建模组和验证组模型C指数评价预测模型的效能。结果白细胞(P=0.028)、淋巴细胞计数(P<0.001)、中性粒细胞(P=0.001)、血小板(P=0.046)、白蛋白(P<0.001)、中性粒细胞/淋巴细胞比值(P<0.001)、血小板/淋巴细胞比值(P<0.001)、淋巴细胞/单核细胞比值(P<0.001)、全身免疫性炎症反应指数(P<0.001)、全身炎症反应指数(P<0.001)、预后营养指数(P=0.004)在患者疗效监测中差异有统计学意义;多因素Cox回归分析显示, 血小板/淋巴细胞比值(HR=2.537, 95%CI 1.439~4.473)和预后营养指数(HR=0.462, 95%CI 0.236~0.903)是预测患者复发转移风险的重要因素, 结合以上指标建立Nomogram风险评估模型, 建模组C指数为0.698, 验证组C指数为0.739, 两组校准曲线一致性良好。结论建立的Nomogram评估模型可准确地预测鼻咽癌患者的复发转移风险, 为临床治疗预后评价提供理论依据。

关键词：鼻咽肿瘤复发转移疗效监测生存分析

APE1 overexpression promotes the progression of ovarian cancer and serves as a potential therapeutic target

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APE1 overexpression promotes the progression of ovarian canc...

第十一届全国免疫学学术大会

作者： Wen Xuemei Lu Renquan Xie Suhong Zheng Hui Wang Yanchun Sun Jiajun Zhang Hongqin zhong ailing Gao Xiang Guo Lin Department of Clinical Laboratory Fudan University Shanghai Cancer Center Department of Oncology Shanghai Medical CollegeFudan University

Objective:Apurinic/apyrimidinic endonuclease 1(APE1) has been recognized as a promising anticancer target in several malignancies,and its selective inhibitors such as APX3330 has been advanced in research ***,the anticancer effect of APE1 against ovarian cancer(OC) has not been well *** this study,we explored the biological function of APE1 in ovarian cancer cells and its anticancer effect as a therapeutic target in ***:Immunohistochemistry(IHC) was used to detect the APE1 protein levels in tissue samples from 12 OC patients and 11 Non-OC *** of APE1 expression was achieved by stable transfection of SKOV3 and A2780 cells with a construct encoding a short hairpin DNA directed against the APE1 ***,cell proliferation,colony formation,cell cycle and apoptosis-associated assays were performed to reveal the biological function of APE1 in ovarian cancer *** addition,SKOV3 and A2780 cells were treated with Camptothecin(CPT) and Ultraviolet Rays(UV) to assess the link between APE1 and ***:Our results showed that APE1 was overexpressed in OC *** of APE1 in A2780 and SKOV3 reduced cell proliferation,arrested cell cycle progression,repressed colony formation and slightly promoted cell apoptosis through BAX and Bcl-2 apoptosis *** addition,down-regulation of APE1 significantly enhanced the sensitivity of ovarian cancer cells to CPT and ***:Our study suggested that APE1 may be essential for the growth of ovarian cancer cells,implicating a potential new therapeutic target for OC.

关键词： APE1 ovarian cancer proliferation therapeutic target apoptosis

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手术室骨科护理专家和专科护士的设置及培训

护士进修杂志 2005年第6期20卷 519-520页

作者：钟爱玲戴红霞练雪萍广东省人民医院手术室广东广州510080

目的探讨手术室骨科护理专家和专科护士的设置及培训方法。方法在手术室设立专科组,按专科化模式培养手术室骨科护理专家和专科护士,建立培训考核体系。结果提高了工作质量,手术医生和病人对手术室的满意度得到提高。结论手术室专科护... 详细信息

目的探讨手术室骨科护理专家和专科护士的设置及培训方法。方法在手术室设立专科组,按专科化模式培养手术室骨科护理专家和专科护士,建立培训考核体系。结果提高了工作质量,手术医生和病人对手术室的满意度得到提高。结论手术室专科护士和临床护理专家的设置培养,能适应外科手术日益专科化发展的需求,提高工作质量和工作效率,值得推广应用。

关键词：手术室骨科临床护理专家专科护士