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Using nanotechnology to prevent fines migration while production

Petroleum 2021年第2期7卷 168-177页

作者： Mohamed Mansour Mohamed Eleraki ahmed noah El-Abbas Moustafa Faculty of Science Zagazig UniversityEgypt Egyptian Petroleum Research Institute(EPRI) Egypt The British University Egypt

Formation damage due to fines migration is a major reason for well productivity decline for oil and gas *** fines are small enough to pass through pore throats causing pore plugging and permeability *** factors affect fines migration such as flow rate,salinity,pH value,reservoir temperature and oil polarity,as well as changes in chemical environment induced by Enhanced Oil Recovery(EOR)*** paper focuses on the effect of flow rates on fines detachment from the grain surfaces,which causing permeability *** the fluid inside the reservoir moves towards the wellbore,the fluid velocity increases,when the fluid reaches the critical flow rate these fines can be picked up into the *** fines captured by thinner pore throats causing pore plugging and permeability *** concentrations of nanoparticles were used to fix these fines on their sources and prevent their mobilization at high flow *** unique technique used in this study is changing the potential surfaces between fines and grain surfaces to prevent fines movement above the critical flow ***_(2)and MgO NPs used in this study can be adsorbed on the pore surfaces and reduce the repulsion forces between fines and pore ***_(2) and MgO nanoparticles at different concentrations(0.25,0.50 and 0.75 g/L)were used on treating the Abu-Rawash sandstone reservoir using Formation Damage System Cell *** experimental studies showed that using MgO NPs would prevent fines detachment from the pore surfaces and decrease the reduction of permeability at high flow rates more than SiO_(2) *** optimum concentration of MgO NPs was at 0.5 g/L as the permeability remediation at this concentration reaches to 64.83%.

关键词： Formation damage Fines migration Nanoparticles Zeta potential Permeability reduction Fluid flow rate

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Validation of a serum microRNA panel as biomarkers for early diagnosis of hepatocellular carcinoma post-hepatitis C infection in Egyptian patients

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World Journal of Gastroenterology 2017年第21期23卷 3864-3875页

作者： Moustafa Nouh Elemeery ahmed noah Badr Marwa Anwar Mohamed Doaa ahmed Ghareeb Medical Biotechnology Laboratory Genetic Engineering and Biotechnology Research DivisionNational Research Centre Toxicology and Food Contaminant Laboratory Food Science and Nutrition Research DivisionNational Research Centre Department of Chemical Pathology Medical Research InstituteAlexandria University Molecular Biology Laboratory Faculty of ScienceAlexandria University

AIM To investigate the prospective importance of serum micro(mi)RNAs(mi R-125 b, mi R-138 b, mi R-1269, mi R-214-5p, mi R-494, mi R375 and mi R-145) as early biomarkers for the diagnosis of hepatitis C virus(HCV)-related hepatocellular carcinoma(HCC).METHODS Two-hundred and fifty HCV4 a patients, 224 HCV4 aHCC patients, and 84 healthy controls were enrolled in the study. Expression levels of mi R214-5p, mi R-125 b, mi R-1269 and mi R-375 were quantified using quantitative real-time *** Expression of the selected mi RNAs in serum wassignificantly lower in HCC patients than in the healthy controls, except for mi R-1269 and mi R-494. There was a significant difference between HCC and HCV patients, in particular for HCC and late stage fibrosis, rather than HCV patients and early fibrosis. It is obvious that mi R-1269 was significantly upregulated in HCC cases compared to hepatic fibrosis cases. Each mi RNA can show HCC progression. Multivariate logistic regression analysis indicated that the tested panel of mi RNAs(mi R214-5p, mi R-125 b, mi R-1269 and mi R-375) represent accurate and specific indictors of HCC *** This study presents a panel of mi RNAs with strong power as putative diagnostic and prognostic biomarkers for HCV-induced HCC. Moreover, mi R-214-5p and mi R-1269 could be considered as early biomarkers for tracking the progress of liver fibrosis to HCC.

关键词： Micro RNA Hepatocellular carcinoma Fibrosis progression

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MicroRNA signature in patients with hepatocellular carcinoma associated with type 2 diabetes

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World Journal of Gastroenterology 2019年第42期25卷 6322-6341页

作者： Moustafa Nouh Elemeery Marwa Anwar Mohamed Marwa ahmed Madkour Mohammed Mohammed Shamseya Noha Mahmoud Issa ahmed noah Badr Doaa ahmed Ghareeb Cheol-Ho Pan Département de Neurosciences CRCHUMUniversitéde MontréalMontréalQuebec H2X 3E4Canada Medical Biotechnology Laboratory Genetic Engineering and Biotechnology Research DivisionNational Research CentreCairo 12622Egypt Natural Product Informatics Research Center KIST Gangneung Institute of Natural ProductsGangneung 25451South Korea Division of Bio-Medical Science and Technology KIST SchoolKorea University of Science and TechnologySeoul 02792South Korea Department of Chemical Pathology Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Experimental and Clinical Internal Medicine Department Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Human Genetics Department Medical Research InstituteAlexandria UniversityAlexandria 21511Egypt Food Toxicology and Contaminates Department National Research CentreDokkiCairo 12622Egypt Bioscreening and preclinical trial lab Biochemistry DepartmentFaculty of ScienceAlexandria UniversityAlexandria 12522Egypt Pharmaceutical and fermentation industries development center the city of scientific research and technological applicationsAlexandria 26411Egypt

BACKGROUND Nonalcoholic steatohepatitis-related cirrhosis is one of the liver complications in type 2 diabetes mellitus(T2DM)and reported to be a risk factor for developing hepatocellular carcinoma(HCC).A reliable screening biomarker of liver cirrhosis(LC)and HCC among T2DM patients is important to reduce the morbidity and mortality of this ***(miRNA)is considered a key player in HCC and T2DM,and it might be a hidden culprit in diabetes-associated HCC,making it a promising reliable prognostic *** To investigate the signature of serum miRNAs as early biomarkers for the screening of HCC among diabetic *** Expression profiles of miRNAs in serum samples of diabetic LC and diabetic HCC patients were assessed using Illumina sequencing;then,RT-qPCR was used to validate significantly altered miRNAs between the two *** miRNAs were tested in serum samples of 200 T2DM patients,270 LC patients,200 HCC patients,and 225 healthy control ***,receiver operating characteristic(ROC)analysis,with area under the curve(AUC),was performed to assess the diagnostic performance of the screened miRNAs for discriminating HCC from LC and nonmalignant patients(LC+T2DM).RESULTS Expression of the sequenced miRNAs in serum was different in HCC vs LCpositive T2DM *** miRNAs(miR-34a,miR-221)were significantly upregulated and five miRNAs(miR-16,miR-23-3p,miR-122-5p,miR-198,miR-199a-3p)were significantly down-regulated in HCC compared to LC *** of ROC curve demonstrated that the combination of these seven miRNAs can be used as a reliable biomarker for detection of HCC in diabetic patients,as it could identify HCC with high diagnostic accuracy in diabetic LC patients(AUC=0.993)and in diabetic nonmalignant patients(AUC=0.961).CONCLUSION This study validates a panel of serum miRNAs that can be used as a reliable noninvasive screening biomarker of HCC among T2DM cirrhotic and noncirrhotic *** study recommends furt

关键词： MicroRNA Hepatocellular carcinoma Type 2 diabetes Nonalcoholic fatty liver disease

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New Pore Pressure Evaluation Techniques for LAGIA-8 Well, Sinai, Egypt as a Case Study

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International Journal of Geosciences 2016年第1期7卷 32-46页

作者： ahmed Zakaria noah Exploration Department Egyptian Petroleum Research Institute Cairo Egypt

Drilling into a geopressured zone will generally cause a change in a number of basic formation/ drilling relationships. This change is usually seen as a reversal of a gradual depth related trend in a lithologically uniform formation. Of all the geophysical methods, the reflection seismic method is essentially the only technique used to predict pore pressures. The seismic method detects changes of interval velocity with depth from velocity analysis of the seismic data. These changes are in turn related to lithology, pore fluid type, rock fracturing and pressure changes within a stratigraphic column. When the factors affecting the velocity are understood for a given area, a successful pressure prediction can be made. For clastic environments such as the Tertiary section of the Gulf of Mexico or the Niger delta, the interval velocity of the rocks increases with depth because of compaction. In these areas, deviations from normal compaction trends are related to abnormally high pore pressures. The adapted methods provide a much easier way to handle normal compaction trend lines. In addition to well log methods, pressure detection can be obtained via drilling parameters by applying Eaton’s DXC methods. Seismic velocities have long been used to estimate pore pressure, indeed both these quantities are influenced by variations in rock properties such as porosity, density, effective stress and so on, and high pore pressure zones are often associated with low seismic velocities. Pressure prediction from seismic data is based on fundamentals of rock physics and seismic attribute analysis. This paper hence tries to assess the use of seismic waves as a viable means to calculate pore pressure, especially in areas where no prior drilling history can be found. Then we applied these methods on LAGIA-8 well, Sinai, Egypt as a case study. Pore pressure prediction from Seismic is a very essential tool to predict pore pressure before drilling operation. This could prevent the well proble

关键词： Pore Pressure Resistivity and Sonic Methods Seismic Velocities Formation Damage

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Investigating the Effect of Various Nanomaterials on the Wettability of Sandstone Reservoir

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World Journal of Engineering and Technology 2015年第3期3卷 116-126页

作者： El-Abbas Moustafa ahmed noah Karam Beshay Lamees Sultan Mina Essam Omar Nouh Egyptian Petroleum Research Institute (EPRI) Cairo Egypt Petroleum and Energy Engineering Department Faculty of Science and Engineering The American University Cairo Egypt

Wettability is the ability of a fluid to stick to a solid surface in the presence of other immiscible fluids. Wettability alteration is crucial as it affects the amount of oil recovered from a given reservoir. The majority of enhanced oil recovery mechanisms purposefully alter the wettability of the reservoir rock from oil-wet to water-wet;to increase the amount of oil recovered from it. This study investigates the effect of various nanomaterials on the wettability, and particularly the brine phase contact angle, of a sand stone reservoir. The nanomaterials used are Magnesium/Aluminum Layered Double Hydroxide, Silica/Zirconia, and a combination of 80.0% Magnesium/Aluminum Layered Double Hydroxide (Mg/Al-LDH) and 20.0% Silica/Zirconia (Zi/Zr). The results suggest that a concentration of 4.0 g/L of Magnesium/Aluminum Layered Double Hydroxide (Mg/Al-LDH) decreases the brine phase contact angle, in the presence of oil, from 66° to 60° in 0.033 minute as opposed to Silica/Zirconia which increases the brine phase contact angle to 68° in the same time interval. The combination of both nanoparticles results in a decrease of 1.0° in the brine phase contact angle indicating that Silica/Zirconia (Zi/Zr) lowers the efficiency of Magnesium/Aluminum Layered Double Hydroxide’s adsorption to the sandstone surface.

关键词： Nanomaterials Wettability Alteration Oil Recovery Mechanism

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Evaluation and Analysis of Oil Shale in Quseir-Safaga and Abu-Tartur Western Desert, Egypt

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Journal of Surface Engineered Materials and Advanced Technology 2014年第2期4卷 53-65页

作者： El-Abbas Moustafa ahmed noah Adel Salem ahmed Nehad Maureen Amir Mohamed Nosr Radwa Awad Mahmoud Gomaa Marawan Moussa Petroleum and Energy Science Department Faculty of Engineering American University in Cairo Cairo Egypt Production Department Egyptian Petroleum Institute EPRI Nasr City Cairo Egypt Petroleum and Energy Science Department Faculty of Engineering American University in Cairo Cairo Egypt

Seeking ways to diminish Egypt’s dependency on foreign oil imports, we had to look for the obvious resources to exploit. Oil shale is one of Egypt’s resources that are left abandoned and not used. This project is aiming at introducing both experimental analysis for oil shale in Egypt and the best production technique for it. This study is about experimentally analyzing different samples of potential oil shale fields in Egypt. Samples that are analyzed in this project are from two locations in Egypt: Quseir-Safaga and Abu-Tartur Western Desert, and they are compared to one of the best oil shale fields in the world, which is from Green River Formation Colorado. The experiments were done using AUC lab retort to experimentally determine the critical temperature that will lead to the maximum production using sudden and gradual change in temperatures, which turned out to be sudden change at 500°C. Thereafter, the degree of API gravity was calculated for each run. Then the oil samples were determined for impurities and liquid chromatography results in the Egyptian Petroleum Research Institute. Part two of the study plan has been included to determine the ultimate ways of producing efficiently, ecologically and economically.

关键词： Oil Shale Retort Method Critical Temperature Oil Composition

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Production of Neural Progenitors from Bone Marrow Mesenchymal Stem Cells

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Stem Cell Discovery 2016年第1期6卷 1-12页

作者： Maeda Mohammad Nahi Yaseen Ahmad Al-Joubory Rafal Abdullah noah Mahmood Aesar A. ahmed ahmed Al-Shammari Experimental Therapy Department Iraqi Center of Cancer and Medical Genetic Research Al-Mustansiriyah University Baghdad Iraq Neuroscience Hospital Baghdad Iraq

In the brain, there are hundreds of types of specialized neurons and to generate one type of them we need to have neural progenitors for differentiation to specific neuron type. Mesenchymal stem cells (MSCs) are easily isolated, cultured, manipulated ex vivo, showing great potential for therapeutic applications. The adult MSCs have the potential to produce progeny that differentiate into a variety of cell types such as neurons. This fact suggests that MSCs derived neurons are an important cell type and a deep understanding of the molecular characteristics of it would significantly enhance the advancement of cell therapy for neurological disorders. Therefore, in this study, we isolated, identified, and studied neural progenitors by measuring expression levels through neurogenesis pathway of three neural differentiation markers nestin (NES), neurofilament (NF-L), and microtubule association protein (MAP-2) from mouse bone marrow MSCs (mouse bmMSCs) by using butylated hydroxyanisole (BHA) and diethyl sulfoxide (DMSO) as neural inducers agents. The results of immunocytochemistry and Real Time-PCR showed that in contrast to MSCs, neural differentiated cells showed neural progenitor pattern by showing stable increase in NES gene expression through differentiation process with increasing the protein expression through different exposures times, while NF-L gene and protein expression start to increased after 48 h but not replaced the NES expression completely even when its expression passed NES levels. The maturation marker Map-2 expression was low during the duration of differentiation period in protein and gene expression, which prove that these cells are still progenitors and can be redirected into specific type of neurons by further treatments.

关键词： BHA Nestin Neural Progenitors Cells MSCs MAP-2

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Investigational growth factors utilized in animal models of spinal fusion: Systematic review

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World Journal of Orthopedics 2019年第4期10卷 176-191页

作者： Ethan Cottrill A Karim ahmed noah Lessing Zachary Pennington Wataru Ishida Alexander Perdomo-Pantoja Sheng-fu Lo Elizabeth Howell Christina Holmes C Rory Goodwin Nicholas Theodore Daniel M Sciubba Timothy F Witham Department of Neurosurgery The Johns Hopkins Hospital Department of Neurosurgery Duke University Medical Center

BACKGROUND Over 400000 Americans annually undergo spinal fusion surgeries, yet up to 40%of these procedures result in pseudoarthrosis even with iliac crest autograft, the current "gold standard" treatment. Tissue engineering has the potential to solve this problem via the creation of bone grafts involving bone-promoting growth factors(e.g., bone morphogenetic protein 2). A broad assessment of experimental growth factors is important to inform future work and clinical potential in this area. To date, however, no study has systematically reviewed the investigational growth factors utilized in preclinical animal models of spinal *** To review all published studies assessing investigational growth factors for spinal fusion in animal models and identify promising agents for *** We conducted a systematic review of the literature using PubMed, Embase,Cochrane Library, and Web of Science databases with searches run on May 29 th,2018. The search query was designed to include all non-human, preclinical animal models of spinal fusion reported in the literature without a timespan limit. Extracted data for each model included surgical approach, level of fusion,animal species and breed, animal age and sex, and any other relevant characteristics. The dosages/sizes of all implant materials, spinal fusion rates,and follow-up time points were recorded. The data were analyzed and the results reported in tables and text. PRISMA guidelines were followed for this systematic *** Twenty-six articles were included in this study, comprising 14 experimental growth factors: AB204(n = 1); angiopoietin 1(n = 1); calcitonin(n = 3);erythropoietin(n = 1); basic fibroblast growth factor(n = 1); growth differentiation factor 5(n = 4), combined insulin-like growth factor 1 +transforming growth factor beta(n = 4); insulin(n = 1); NELL-1(n = 5); noggin(n= 1); P-15(n = 1); peptide B2 A(n = 2); and secreted phosphoprotein 24(n = 1).The fusion rates of the current gold sta

关键词： Spinal fusion Growth factor Pseudoarthrosis Systematic review

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