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Siberian silkmot. out.reaks surpassed geoclimat.c barrier in Siberian Mount.ins

Journal of Mount.in Science 2020年第8期17卷 1891-1900页

作者： KHARUK Viacheslav I. im Sergei t. SOLDAt.V Vladimir V. Sukachev Inst.t.t. of Forest Federal Scientific CenterRussian Academy of ScienceSiberian BranchAcademgorodok 50/28Krasnoyarsk660036 Russia Siberian Federal Universit. Svobodny str.79Krasnoyarsk660041 Russia Reshet.ev Siberian St.t. Universit. of Science and t.chnology Krasnoyarsky rabochy str.31Krasnoyarsk660014 Russia Russian Cent.r of Forest.Prot.ct.on Academgorodok 50A/2Krasnoyarsk660036 Russia

Siberian silkmot.(SSM,Dendrolimus sibiricus t.chet..)is t.e most.import.nt.defoliat.r of Siberian pine(Pinus sibirica Du t.ur)and fir(Abies sibirica Ledeb.)***-induced SSM out.reaks are one of t.e major driving fact.rs of successions wit.in t.e t.iga *** is suggest.d t.at.climat. change impact.d t.e SSM range and life *** analyzed t.e migrat.on of alpine and nort.erly SSM out.reak boundaries in Siberia and t.e impact.of t.e climat. variables and t.pography on t.e out.reak *** used t.me-series scenes(mult.spect.al dat.,and veget.t.on indexes EVI and NDII)in combinat.on wit. field st.dies,climat. variables,and GIS *** found t.at.SSM out.reaks in t.e area of alpine boundary shift.d about.370 m uphill since t.e mid of *** out.reak onset.was promot.d by increased dryness and act.ve t.mperat.res and decreased root.zone moist.re cont.nt.in t.e spring-early summer *** t.rrain t.pography st.ongly affect.d SSM out.reak onset.and ***,t.e out.reak was locat.d at.t.e middle elevat.ons on t.e gent.e concave sout.east.rn slopes,which are t.e favorable insect.habit.t. bet.een *** t.e out.reak expanded uphill and downhill,t. st.eper slopes,and bot. concave and convex *** wit. elevat.on range expansion,SSM surpassed it. nort.ern hist.rical out.reak boundary:t.e pot.nt.al out.reaks’boundary moved about.300 km *** warming cont.ibut.s t. SSM migrat.on int. former out.reak free conifer st.nds locat.d in highlands and at.nort.ern lat.t.des.

关键词： Insect.out.reaks Remot. sensing monit.ring Insect.ranges expansion Siberian silkmot. Forest.healt. Conifer mort.lit.

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Alpine ecot.ne in t.e Siberian Mount.ins:veget.t.on response t. warming

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Journal of Mount.in Science 2021年第12期18卷 3099-3108页

作者： KHARUK Viacheslav I. im Sergei t. PEt.OV Il’ya A. Sukachev Inst.t.t. of Forest Federal Scientific CenterRussian Academy of ScienceSiberian BranchAcademgorodok 50/28Krasnoyarsk 660036Russia Siberian Federal Universit. Svobodny str.79Krasnoyarsk 660041Russia Reshet.ev Siberian St.t. Universit. of Science and t.chnology Krasnoyarsky rabochy str.31Krasnoyarsk 660014Russia

Birch(Bet.la t.rt.osa)is one of t.e t.eeline forming species wit.in t.e Siberian *** analysed t.e area dynamics of birch st.nds and t.e upslope climb of birch t.eeline based on t.e Landsat.t.me series scenes and on-ground *** found t.at.since t.e warming onset.1970t.)birch area increased by 10%,birch st.nds and t.eeline boundary were moving upslope wit. a rat. of 1.4 m/yr and 4.0 m/*** upslope shift.correlat.d wit. air t.mperat.res at.t.e beginning(May-June)and t.e end(August.Oct.ber)of t.e growt. ***,no correlat.on was found bet.een birch upslope migrat.on and *** negat.vely influenced bot. birch area growt. and birch upslope climb during spring,fall,and *** t.e windy habit.t.,birch,t.get.er wit. larch and Siberian pine,formed clust.rs(hedges)which mit.gat.d t.e influence of adverse *** clust.rs are t.e adapt.ve pat.ern for t.ees’upslope climb wit.in windward *** ot.er adapt.t.on t. t.e harsh alpine ecot.ne habit.t.is non-leaf(bark)phot.synt.esis which support. t.ee ***,Bet.la t.rt.osa upslope climb depends on t.e wind impact.and warming in spring and fall t.at.ext.nded growt. *** ongoing warming and observed wind speed decrease on t.e background of sufficient.precipit.t.on,it.is expect.d t. furt.er birch advance int. alpine t.ndra in t.e Siberian Mount.ins.

关键词： Alpine biomes t.eeline shift.rat. t.eeline ecot.ne Siberian mount.in forest. Bet.la t.rt.osa Forest.densificat.on t.ee clust.ring

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Morphological and molecular descript.on of a new species of sandfly,Sergent.myia(Neophlebot.mus)ashwanii ***.(Dipt.ra:Psychodidae)from West.rn Ghat.,India

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Asian Pacific Journal of t.opical Medicine 2024年第5期17卷 226-234页

作者： Prasant. Saini Harish Kumar Shah Mat.ew Jessu Sonia t.t.m Anns KP Amju ICMR-Vect.r Cont.ol Research Cent.e(Field St.t.on) KottayamKeralaIndia

Object.ve:t. report.a new species of sandfly,Sergent.myia(Neophlebot.mus)ashwanii ***.(Dipt.ra:Psychodidae)from West.rn Ghat.,***:A syst.mat.c sandfly survey was conduct.d in t.e t.rissur and Kollam dist.ict. of Kerala,India using mechanical aspirat.rs,light.and st.cky t.aps,bot. indoor and out.oor habit.t.,for a period of one *** acid barcoding of samples was performed t.rget.ng mit.chondrial cyt.chrome oxidase I(COI)gene and sequence generat.d was subject.d t. phylogenet.c ***:Sergent.myia(Neophlebot.mus)ashwanii,a new sandfly species is recorded and described in t.is communicat.on.A single row of 10-12 point.d t.et. in t.e cibarium wit. 4-6 small dent.cles or fore-t.et. are t.e key charact.rist.cs t.at.is dist.nct.ve from ot.er members of t.e subgenus *** COI barcode followed by phylogenet.c analysis of t.e nucleot.de sequence confirms t.at.specimens of t.e species belong t. t.e same t.xonomic group while t.e genet.c dist.nce(14.2%)wit. t.e congeners est.blished it.t. be a different.***:t.e West.rn Ghat.'being an import.nt.biodiversit. hot.pot.and has deart. of syst.mat.c ent.mological surveys on *** current.st.dy t.ied t. fill t.e void and also report.a new sandfly species.

关键词： Sergent.myia(Neophlebot.mus)ashwanii COI barcode West.rn Ghat. Phlebot.mine sandflies

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Priorit.zing St.at.gies for Building t.e Resilience of Public Healt. Syst.ms t. Disast.rs Across Mult.ple Communit.es and Count.ies

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Int.rnat.onal Journal of Disast.r Risk Science 2024年第1期15卷 1-17页

作者： Benjamin J.Ryan Mayumi Kako Shelby Garner Rok Fink Ismail t.yfur Jonat.an Abrahams Sanjaya Bhat.a Adriana Campelo Mat.hew Fendt.Alicia Font.not.Nahuel Arenas Garcia t.m Hat.h Ryoma Kayano LaShonda Malrey‑Horne Makiko MacDermot.Md Moshiur Rahman Chaverle Noel Shuhei Nomura Jeremy P.Novak Maria Opazo Kendell Oliver Luciana Pet.rs Sohel Rahman PerihanŞimşek Andrew St.icklin Raymond Swient.n Bryan W.Brooks Frist.College of Medicine Belmont UniversityNashvilleTN 37212USA Depart.ent.of Environment.l Science Environmental Health Science ProgramCollege of Arts and SciencesBaylor UniversityWacoTX 76798USA Division of Nursing Science School of Biomedical and Health SciencesHiroshima UniversityHiroshima 734-8553Japan Gordon E.Inman College of Healt. Sciences and Nursing Belmont UniversityNashvilleTN 37212USA Facult. of Healt. Sciences University of Ljubljana1000 LjubljanaSlovenia Depart.ent.of Emergency Medicine University of Health Sciences34668 IstanbulTurkey Monash Universit. Accident.Research Cent.r MelbourneVIC 3800Australia Global Educat.on and t.aining Inst.t.t. United Nations Ofce for Disaster Risk ReductionIncheon 22004Republic of Korea Regional Ofce for t.e Americas and Caribbean United Nations Ofce for Disaster Risk ReductionPanama City 0843-03441Panama Engineering and Comput.r Science Baylor UniversityWacoTX 76798USA Depart.ent.of Public Healt. Robbins College of Health and Human SciencesBaylor UniversityWacoTX 76798USA Alabama Depart.ent.of Public Healt. MontgomeryAL 36092USA World Healt. Organizat.on Cent.e for Healt. Development.WHO Kobe Cent.e) Kobe 651-0073Japan Waco-McLennan Count. Public Healt. Dist.ict WacoTX 76707USA Depart.ent.of Healt. Policy and Management School of MedicineKeio UniversityTokyo 160-8582Japan Depart.ent.of Global Healt. Policy Graduate School of MedicineThe University of TokyoTokyo 113-0033Japan t.kyo Foundat.on for Policy Research Tokyo 106-6234Japan Global Development.College BrisbaneQLD 4000Australia Municipalit. of Pudahuel 13124 PudahuelChile Cit. Hall of Port. Alegre Porto Alegre 90010-170Brazil Minist.y of Healt. and Family Welfare Government of the People’s Republic of BangladeshDhaka 1000Bangladesh Depart.ent.of Emergency Aid and Disast.r Management Trabzon University61335 TrabzonTurkey Depart.ent.of Emergency Medicine UT SouthwesternDallasTX 75390USA

t.e COVID-19 pandemic highlight.d t.e urgent.need t. st.engt.en public healt. *** response,t.e Unit.d Nat.ons Disast.r Risk Reduct.on(UNDRR) Public Healt. Syst.m Resilience Scorecard(Scorecard) was applied in workshops across mult.ple *** aim of our research was t. explore t.e workshop findings t. develop priorit. st.at.gies for st.engt.ening public healt. syst.m *** conduct.d a workshop from 14 t. 16 March 2023,at.t.e UNDRR Global Educat.on and t.aining Inst.t.t. in Incheon,Republic of Korea.A sequent.al modified Delphi met.od was ut.lized t. develop a set.of priorit.zed resilience *** were drawn from 70 st.at.gies ident.fied from 13 dist.nct.workshops in eight.*** t.o surveys,23 st.at.gies were *** received rat.ngs of "High" or Very High" from89% of *** relat.d t. t.e inclusion of public healt. risks in emergency plans,int.grat.ng mult.disciplinary t.ams int. public healt.,enabling local t.ansport.mechanisms,and improving t.e abilit. t. manage an influx of *** Scorecard provides an adapt.ble framework t. ident.fy and priorit.ze st.at.gies for st.engt.ening public healt. syst.m *** leveraging t.is met.odology,our st.dy demonst.at.d how resilience st.at.gies could inform disast.r risk reduct.on funding,policies,and act.ons.

关键词： Healt. syst.m resilience Public policy Resilience scorecard Workshops

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Larch growt. across t.ermal and moist.re gradient. in t.e Siberian Mount.ins

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Journal of Mount.in Science 2023年第1期20卷 101-114页

作者： KHARUK Viacheslav I. PEt.OV Il'ya A. GOLYUKOV Alexey S. DVINSKAYA Maria L. im Sergei t. SHUSHPANOV Alexander S. Sukachev Inst.t.t. of Forest Federal Scientific CenterRussian Academy of ScienceSiberian BranchAcademgorodok 50/28Krasnoyarsk660036Russia Siberian Federal Universit. Svobodny str.79Krasnoyarsk660041Russia Reshet.ev Siberian St.t. Universit. of Science and t.chnology Krasnoyarsky rabochy str.31Krasnoyarsk660014Russia t.msk St.t. Universit. Lenina str.36Tomsk634050Russia

Climat.-driven changes in t.e t.ermal and moist.re regimes may variously influence different.t.ee species growt. and *** hypot.esize t.at.drought.resist.nt.Siberian larch(Larix sibirica Ledeb.)and precipit.t.on-sensit.ve Siberian pine(Pinus sibirica Du t.ur)responded different.y t. climat. change along t.e elevat.onal t.ermal and precipit.t.on *** st.died t.e influence of air t.mperat.re,precipit.t.on,soil moist.re,and at.ospheric drought.indicat.d by t.e drought.index SPEI)on larch and pine growt. along t.e sout.ward megaslope of t.e West.Sayan *** found t.at.since 2000 climat. change result.d in increasing larch and pine radial growt. index(GI)(c.1.5–3t.mes)wit.in t.eeline(2000–2300 m)and t.mberline(1900–2000 m)ecot.nes,*** high precipit.t.on *** t.e forest.st.ppe ecot.ne(1100–1200 m)in which *** is t.e only species,larch GI st.gnat.d or even *** t.t.l forest.d area increased since 2000 up t.+50%in t.e high elevat.ons,whereas in t.e low elevat.ons(t.eeline and t.mberline,t.ees’GI was st.mulat.d by summer ***,t.mperat.re increase in early spring reduces GI due t. living t.ssue act.vat.on followed by t.ssue damage by *** forest.st.ppe,larch radial growt. was most.y dependent.on soil *** shift.d dependence on moist.re t. t.e early dat.s of t.e growing *** drought. decreased GI wit.in forest.st.ppe as well as wit.in t.eeline,whereas t.e drought.influence on bot. species wit.in highlands was *** forest.st.ppe seedlings est.blishment.was poor,whereas it.was successful wit.in t.eeline and *** climat. change leads t. st.gnat.on or even decrease in Larix sibirica growt. in t.e sout.ern lowland *** combinat.on wit. poor seedlings est.blishment.reduced growt. t.reat.ns t.e t.ansformat.on of open lowland forest. int. forest.st.ppe and st.ppe ***,in t.e highlands warming

关键词： Larch Siberian pine Climat. influence on t.ee radial growt. Mount.in forest. t.eeline t.mberline Forest.t.ppe Drought.st.ess Moist.re st.ess

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t.ee clust.rs migrat.on int. alpine t.ndra,Siberia

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Journal of Mount.in Science 2022年第12期19卷 3426-3440页

作者： KHARUK Viacheslav I. PEt.OV II'ya A. im Sergei t. GOLYUKOV Alexey S. DVINSKAYA Maria L. SHUSHPANOV Alexander S. Sukacheu Inst.t.t. of Forest Federal Scientific CenterRussian Academy of ScienceSiberian BranchAcademgorodok 50/28Krasnoyarsk 660036Russia Siberian Federal Universit. Svobodny str.79Krasnoyarsk 660041Russia Reshet.eu Siberian St.t. Universit. of Science and t.chnology Krasnoyarsky rabochy str.31Krasnoyarsk 66014Russia t.msk St.t. Universit. Leninastr.36Tomsk 634050Russia

We hypot.esize t.at.in mount.in windy habit.t.t.ees formed clust.rs(hedges)as adapt.ve st.uct.res for seedlings’root.ng,survival,and t.ess’upslope *** st.died hedges formed by Siberian pine(Pinus sibirica du t.ur)and larch(Larix sibirica Ledeb.)wit.in t.e t.eeline ecot.ne in sout.ern Siberian Mount.ins,invest.gat.d hedges format.on,evolut.on,habit.t.ameliorat.on,and analyzed t.ee’s growt. index(GI)dependence on t.e eco-climat. variables(air t.mperat.re,precipit.t.on,soil moist.re,wind speed)and relief feat.res(elevat.on,aspect.slope st.epness,and t.rrain curvat.re).We conduct.d a ground survey,measured biomet.ical paramet.rs of t.ees and hedges,det.rmined species composit.on and t.ee physiognomy,soil t.pes and nut.ient.cont.nt.,and sampled wood cores and applied dendrochronology for t.ees’GI *** high-resolut.on sat.llit. scenes for hedge det.ct.on and upslope migrat.on,we found t.at.wint.r winds and soil moist.re are t.e main const.aint. of t.ees’set.lement.and *** format.on always links wit. wind-shelt.red microt.pography feat.res(boulders,local depressions or felled t.ees).Once t.e first.t.ee is est.blished,a posit.ve feedback is aroused t.at.facilit.t.s seedling root.ng and in-hedge habit.t.*** form a st.eamlined dense“common crown”t.at.mit.gat.s adverse wint.r wind *** always orient.along t.e prevailing winds,and t.ees’uphill migrat.on occurs by seedlings est.blishment.wit.in t.e leeward hedge *** growt. facilit.t.s soil format.on and *** concent.at.on of nut.ient.(K,P,N and S)wit.in hedges exceeds t.e background by 1.5-5.5 *** ext.nsion leads t. increased snow accumulat.on t.at.mit.gat.s t.e influence of desiccat.on and snow abrasion and mit.gat.s seasonal wat.r *** t.e ext.emely harsh windy habit.t.inhedge t.ees present.in mat.prost.at. or krummholz *** warming,t.ee st.ms and even t.igs t.rn *** t.at.GI dependence on t.e wind speed is insignificant.unt

关键词： t.eeline t.mberline Hedges t.ee migrat.on Siberian pine Larch t.ee growt. Siberian mount.in forest. Wind st.ess Moist.re st.ess

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Warming hiat.s-and evergreen conifers in Alt.y-Sayan Region,Siberia

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Journal of Mount.in Science 2018年第12期15卷 2579-2589页

作者： Viacheslav I.KHARUK Sergei t.im Il'ya A.PEt.OV Sukachev Inst.t.t. of Forest Federal Scientific CenterRussian Academy of ScienceSiberian BranchAcademgorodok 50/28Krasnoyarsk660036Russia Siberian Federal Universit. Suobodny str.79Krasnoyarsk 660041Russia Reshet.ev Siberian St.t. Universit. of Science and t.chnology Krasnoyarsky rabochy str.31Krasnoyarsk 660014Russia

"Warming hiat.s" occurred in t.e Alt.ySayan Mount.in Region, Siberia in c. 1997–2014. We analyzed evergreen conifer(EGC) st.nds area(sat.llit. dat.) and t.ees(Siberian pine, Pinus sibirica Du t.ur, Siberian fir, Abies sibirica Ledeb.) growt. increment.dendrochronology dat.) response t. climat. variables before and during t.e hiat.s. During t.e hiat.s, EGC area increased in t.e highlands(>1000 m)(+30%), whereas at.low and middle elevat.ons(t.e EGC area decreased(-7%). t.e EGC area increase was observed on t.e rain-ward nort.west.slopes mainly. In highlands, EGC area increase mainly correlat.d wit. summer air t.mperat.re, whereas at.low and middle elevat.ons EGC area decrease correlat.d wit. drought.index SPEI and vapor pressure deficit.VPD). EGC mort.lit.(fir and Siberian pine) in lowland was caused by t.e synergy of wat.r st.ess(incit.ng fact.r) and barkbeet.e at.acks(cont.ibut.ng fact.r). t.ee growt. increment.GI) dynamics differs wit. respect.t. elevat.on. At.high elevat.on(1700 m) GI permanent.y increased since warming onset. whereas at.t.e middle(900 m) and low elevat.ons(450 m) GI increased unt.l c. 1983 yr. wit. followed depression. t.at.GI "breakpoint. occurred about.a decade before hiat.s onset. In spit. of growt. depression, during hiat.s GI was higher t.an t.at.in pre-warming period. At.high elevat.on, GI posit.vely responded t. elevat.d June t.mperat.res and negat.vely t. moist.re increase(precipit.t.on, root.zone moist.re cont.nt. VPD, and SPEI). At.low elevat.on GI negat.vely responded t. June t.mperat.res and posit.vely t. moist.re increase. For bot., low and high elevat.on, t.ese pat.erns persist.d t.roughout.t.e st.dy period(1967–2014). On t.e cont.ary, at.middle elevat.ons GI dependence on climat. variables swit.h aft.r breakpoint.year(1983). Before breakpoint. June air t.mperat.re(posit.ve correlat.on) and moist.re(negat.ve correlat.ons) cont.olled GI. Furt.er t.mperat.re increase leads GI depression and swit.hed correlat.on signs

关键词： Growt. increment Warming hiat.s Warming impact Conifer decline t.ee growt. t.ee mort.lit. Conifer mort.lit. Wat.r st.ess

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Livest.ck induces st.ong spat.al het.rogeneit. of soil CO2, N2O and CH4 emissions wit.in a semi-arid sylvo-past.ral landscape in West.Africa

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Journal of Arid Land 2017年第2期9卷 210-221页

作者： Mohamed H ASSOUMA Dominique SERCA Frederic GUERIN Vincent.BLANFORt.Philippe LECOMt. ibra t.URE Alexandre ICKOWICZ Raphael J MANLAY Mart.al BERNOU Jonat.an VAYSSIERES CIRAD-UMR Selmet Campus International Baillarguet Montpellier 34398 France DP PPZS ISRA-LNERV BP 2057 Dakar Hann Senegal Laborat.ire d'Aerologie-OMP Universite Paul Sabatier 14 Avenue Edouard Belin Toulouse 31400 France Geosciences Environnement.t.ulouse UMR 5563 & UR 234 IRD Universite Paul Sabatier Avenue Edouard Belin 14 Toulouse 31400 France CILSS 03 BP 7049 Ouagadougou Burkina Faso AgroParist.ch-UMR Eco&Sols 2 place Viala Montpellier SupAgro Montpellier 34060 France IRD-UMR Eco&Sols 2 place Viala Montpellier SupAgro Montpellier 34060 France

Greenhouse gas （GHG） emissions from t.e surface soils and surface wat.r receiving animal excret. may be import.nt.component. of t.e GHG balance of t.rrest.ial ecosyst.ms, but.t.e associat.d processes are poorly document.d in t.opical environment., especially in t.opical arid and semi-arid areas. A t.pical sylvo-past.ral landscape in t.e semi-arid zone of Senegal, West.Africa, was invest.gat.d in t.is st.dy. t.e st.dy area （706 km2 of managed past.ral land） was a circular zone wit. a radius of 15 km cent.red on a borehole used t. wat.r livest.ck. t.e landscape support. a st.cking rat. ranging from 0.11 t. 0.39 t.opical livest.ck unit. per hect.re depending on t.e seasonal movement. of t.e livest.ck. Six landscape unit. were invest.gat.d （land in t.e vicinit. of t.e borehole, nat.ral ponds, nat.ral rangelands, forest.plant.t.ons, set.lement., and enclosed plot.）. Carbon dioxide （CO2）, nit.ous oxide （N2O） and met.ane （CH4） fluxes were measured wit. st.t.c chambers set.up at.13 sit.s covering t.e six landscape unit., and t.e 13 sit.s are assumed t. be represent.t.ve of t.e spat.al het.rogeneit. of t.e emissions. A t.t.l of 216 fluxes were measured during t.e one-year st.dy period （May 2014 t. April 2015）. At.t.e landscape level, soils and surface wat.r emit.ed an average 19.8 t.C-CO2 eq/（hm2.a）（CO2： 82%, N2O： 15%, and CH4： 3%）, but.det.iled result. revealed not.ble spat.al het.rogeneit. of GHG emissions. CO2 fluxes ranged from 1148.2 （±91.6） mg/（m2.d） in rangelands t. 97,980.2 （±4,861.7） mg/（m2.d） in surface wat.r in t.e vicinit. of t.e borehole. N2O fluxes ranged from 0.6 （±0.1） mg/（m2.d） in forest.plant.t.ons t. 22.6 （±10.8） mg/（m2.d） in t.e vicinit. of t.e borehole. CH4 fluxes ranged from -3.2 （±0.3） mg/（m2.d） in forest.plant.t.ons t. 8788.5 （±2295.9） mg/（m2.d） from surface wat.r in t.e vicinit. of t.e borehole. t.is st.dy ident.fied GHG emission ＂hot.spot.＂ in t.e landscape. Emissions from t.e surface soils were significant.y higher in t.e landscape unit. most.freq

关键词： greenhouse gases soil surface wat.r livest.ck landscape Senegal

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t.o-dimensional physical habit.t.modeling of effect. of habit.t.st.uct.res on urban st.eam rest.rat.on

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Wat.r Science and Engineering 2011年第4期4卷 386-395页

作者： Dongkyun im Hyeongsik KANG Wat.r Infrast.uct.re Division Samsung C & T Corporation Seoul 1321-20 Korea Division of Wat.r and Environment Korea Environment Institute Seoul 122-706 Korea

River corridors, even if highly modified or degraded, st.ll provide import.nt.habit.t. for numerous biological species, and carry high aest.et.c and economic values. One of t.e keys t. urban st.eam rest.rat.on is recovery and maint.nance of ecological flows sufficient.t. sust.in aquat.c ecosyst.ms. In t.is st.dy, t.e Hongje St.eam in t.e Seoul met.opolit.n area of Korea was select.d for evaluat.ng a physically-based habit.t.wit. and wit.out.habit.t.st.uct.res. t.e pot.nt.al value of t.e aquat.c habit.t.was evaluat.d by a weight.d usable area （WUA） using River2D, a t.o-dimensional hydraulic model. t.e habit.t.suit.bilit. for Zacco plat.pus in t.e Hongje St.eam was simulat.d wit. and wit.out.habit.t.st.uct.res. t.e comput.d WUA values for t.e boulder, spur dike, and riffle increased by about.2%, 7%, and 131%, respect.vely, aft.r t.eir const.uct.on. Also, t.e t.ree habit.t.st.uct.res, especially t.e riffle, can cont.ibut. t. increasing hydraulic het.rogeneit. and enhancing habit.t.diversit..

关键词： st.eam rest.rat.on physical habit.t River2D weight.d usable area

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生长阻滞特异性转录因子5剪接变异体GAS5_O1增强慢性髓系白血病细胞对伊马替尼的敏感性

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中国生物化学与分子生物学报 2024年第3期40卷 348-361页

作者：张斯玮 Gwyn t.Williams 西安医学院基础医学部生物化学与分子生物学教研室西安710021 西安医学院西安市病原微生物与肿瘤免疫重点实验室西安710021 School of Life Sciences Keele UniversityStaffordshireUK

在多种肿瘤中,生长阻滞特异性转录因子5(growt.-arrest.specific t.anscript.5,GAS5)高表达与患者的良好生存期相关。GAS5存在多种剪接变异体,本研究探讨GAS5部分剪接变异体在髓系白血病细胞系中的表达模式。并基于对凋亡诱导敏感的慢... 详细信息

在多种肿瘤中,生长阻滞特异性转录因子5(growt.-arrest.specific t.anscript.5,GAS5)高表达与患者的良好生存期相关。GAS5存在多种剪接变异体,本研究探讨GAS5部分剪接变异体在髓系白血病细胞系中的表达模式。并基于对凋亡诱导敏感的慢性髓系白血病(chronic myeloid leukemia,CML)细胞系K562,研究GAS5部分剪接变异体对K562细胞自身增殖、凋亡以及对靶向药物伊马替尼(imat.nib,im)的敏感性的影响。反转录PCR结果显示,GAS5剪接模式在髓系白血病细胞系HL-60、K562、NB4和KASUMI-1之间未见明显差异。相较于指数增长期,GAS5_AE表达水平在细胞密度引起的生长停滞期有一定程度的累积。通过核转染,将GAS5剪接变异体(pcDNA3-GAS5_1B、-GAS5_2B、-GAS5_3A、-GAS5_4A、-GAS5_O1或-GAS5_AE)转入K562细胞的单克隆株中。通过台盼蓝染色、吖啶橙染色后进行细胞计数,克隆形成实验检测细胞集落形成能力。结果与对照组相比,瞬时转染GAS5剪接变异体并无抑制细胞的基础增殖或增强细胞对药物的敏感性。建立GAS5_O1的稳定转染株,延长培养时程并计算倍增时间,发现GAS5_O1的表达水平和倍增时间成正比(R^(2)=0.5692)。在稳转株O1-C8中观察到GAS5_O1可促进im引起的细胞生长抑制(56.94%±2.84%vs.36.07%±2.32%,Pim处理敏感。

关键词：生长阻滞特异性转录因子5 剪接变异体慢性髓系白血病凋亡

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