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Soil Phosphorus Fractions Change in Winter in a Corn-Soybean Rotation with Tillage and Phosphorus Fertilization

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Pedosphere 2015年第1期25卷 1-11页

作者： Yi-Chao SHI Noura ZIADI Aimé J.MESSIGA Roger lalande HU Zheng-Yi Soils and Crops Research and Development Centre Agriculture and Agri-Food Canada (AAFC) College of Resources and Environment University of Chinese Academy of Sciences Environmental and Resource Studies Program Trent University

Determining how agricultural management practices affect soil phosphorus(P) over the winter may further our understanding of the soil P cycle under specific environmental conditions in eastern Canada. This study assessed changes over winter for soil P fractions and other selected chemical properties as affected by tillage and P fertilization. In 1992, a long-term corn(Zea mays L.) and soybean(Glycine max L.) rotational experiment was established in the province of Quebec, Canada. Soil samples(0–15 cm) were collected in fall 2001 and 2007 after a soybean harvest, and in the following spring 2002 and 2008 before corn seeding, in main plots under moldboard plow and no-till managements and selected subplots fertilized with 0, 17.5, or 35 kg P ha-1and 160 kg N ha-1. Soil samples were analyzed for P fractions and other chemical properties to assess changes over winter for 2001–2002 and 2007–2008. Changes over winter of all soil P fractions were significant for the two periods, indicating the occurrence of soil P transformation and movement over winter. The Mehlich-3-extractable Fe, Al, Ca, and Mg decreased during the two studied periods. Tillage had no significant effect on all soil P fractions. The resin-extractable P in 2001–2002 and Na HCO3-extractable inorganic P and Na OH-extractable organic P during the two winters were significantly increased under P fertilization. This study demonstrated that P in cultivated soils changed during winter as a result of changes in labile P fractions possibly due to the solubilization of residual fertilizer P combined with environmental factors.

关键词： agricultural management practices environmental factors inorganic P labile P organic P

Comparison of the Bacterial Microbiota in a Bale of Collected Cardboard Determined by 454 Pyrosequencing and Clone Library

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Advances in Microbiology 2014年第12期4卷 754-760页

作者： Valérie lalande Simon Barnabé Jean-Charles Coté Département de Biologie Médicale Université du Québec à Trois-RivièresTrois-RivièresCanada Agriculture and Agri-Food Canada Research and Development CentreSaint-Jean-sur-RichelieuCanada Département de Chimie Biochimie et PhysiqueUniversité du Québec à Trois-RivièresTrois-RivièresCanada

Biofouling, the accumulation of microorganisms, is a major problem in paper mills processing paper and cardboard. This leads to the production of lower quality recycled products. Several studies have focused on the microbial content in the paper mill and the final products. Our aim was to determine the microbial biota in a bale of collected cardboard prior to entering the paper mill. Total genomic DNA was isolated and analyzed using two different methods for comparison purposes: 454 pyrosequencing and clone library. A total of 3268 V6-V8 454 pyrosequencing reads and 322 cloned V6-V8 16S rRNA nucleotide sequences were obtained. Both methods showed the presence of three major bacterial genera: Bacillus, Solibacillus and Paenibacillus, all members of the spore-forming phylum Firmicutes. Pyrosequencing, however, revealed a richer and more diverse bacterial community than clone library. It showed the presence of additional minor Firmicute genera and of a small number of Proteobacteria. The sorting at the recycling plant, the storing, and the processing at the paper mill, the end uses, will all contribute to the bacterial microbiota present in a bale of collected cardboard as revealed here.

关键词： 454 Pyrosequencing Bacterial Microbiota Cardboard Clone Library Paper Mill

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小儿造口护理

小儿造口护理

长期耕作和施磷对玉米生长、土壤理化性质和AM真菌特性的影响

中华护理学会第9届全国造口、伤口、失禁护理学术交流会议、中华护理学会全国外科护理学术交流会议、中华护理学会全国神经内、外科护理学术交流会议

作者： Lauise Forest-lalande RN.M.Ed.ET

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长期耕作和施磷对玉米生长、土壤理化性质和AM真菌特性的影响

感染HIV儿童和青少年在高活性抗逆转录病毒治疗下的生活质量

第七次全国土壤生物与生物化学学术研讨会暨第二次全国土壤健康学术研讨会

作者：盛敏 Roger lalande Noura Ziadi Chantal Hamel 唐明王春燕西北农林科技大学林学院 Soils and Crops Research and Development Centre Agriculture and Agri-Food Canada2560 Hochelaga Blvd.Quebec CityQCCanadaG1V 2J3 Semiarid Prairie Agricultural Research Centre Agriculture and Agri-Food CanadaBox 1030Airport Rd.Swift CurrentSKS9H 3X2

丛枝菌根(Arbuscular Mycorrhiza,AM)真菌是重要的土壤生物成员之一,其在促进作物生长、提高作物抗逆性等方面具有重要作用,但这些作用的发挥也同时受农业措施、土壤因子和环境条件等诸多因素的影响和制约。现代农业中化学肥料的大量使...

丛枝菌根(Arbuscular Mycorrhiza,AM)真菌是重要的土壤生物成员之一,其在促进作物生长、提高作物抗逆性等方面具有重要作用,但这些作用的发挥也同时受农业措施、土壤因子和环境条件等诸多因素的影响和制约。现代农业中化学肥料的大量使用和机械化程度的增加已引起土壤水蚀、酸化、渗透能力降低等诸多生态

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世界核心医学期刊文摘（儿科学分册） 2006年第8期2卷 56-57页

作者： Thni G.J. lalande M. Bachelard G. 郭战宏 Service de Pédiatrie III CHU A.de Villeneuve371avenue du Doyen-Giraud34059 MontpellierFrance

Objective.-To evaluate Quality of life(QoL)of HIV-infected children under highly active antiretroviral therapies,and its change over 18 *** s and methods.-QoL was evaluated by self-administred questionnaires(french v ersions of AUQUEI,OK-ado,and compilation of both)in 23 young living with HIV /AIDS(6-15 yrs),under antiretroviral multitherapies,and re-evaluated 18 mon ths later in 19 of ***.-At baseline,QoL in HIV-infected children-adolescents was relatively *** answers given to each items and the mean sc ore from infected children were similar to those obtained in uninfected healthy ***,infected adolescents distinguished definitly from healthy ad olescents,describing higher *** mean satisfaction score from the whole gro up decreased between M0 and M18(mainly in the youngests)-,and mainly concerne d 3 fields(self-esteem,health and school)while their somatic health remained stable or *** effect of the familial context was also observed for the se 3 ***.-The progressive decline of QoL in HIV-infected child ren,and the surprising high and steady level of satisfaction over-time provide d by the adolescents,underlined the frailty of this population and the need for a psychologic management associated to the medical *** a multidisc iplinary approach should take into account the preoccupations and difficulties o f each age-class,those linked to the diagnosis itself,and to the familial or scolar contexts,in order to preserve QoL of this pediatric population,as far a s possible,in a long term.

关键词：高活性抗逆转录病毒治疗 HIV/AIDS 健康青少年健康儿童生活质量感染抗逆转录酶病毒治疗 QoL 自我评价躯体健康

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一个为期4年的儿童沙门菌感染的回顾性研究

世界核心医学期刊文摘（儿科学分册） 2005年第6期1卷 19-19页

作者： lalande M. Guyon G. Morin C. 刘健

Objectives. -Epidemiological state of salmonella infections in Languedoc-Roussillon (France) and discussion of therapeutic indications. Material and method. -Retrospec- tive study over a four-year period of paediatric salmonella infections requiring hospitalisation in Montpellier University Hospital. Analysis of clinical, bacteriological, laboratory and therapeutic data. Results. -One hundred and sixty nine cases of paediatric salmonella infections have been investigated. The clinical triad consisted of diarrhea, abdominal pain and fever. Six severe cases have been observed and seemed to have been independent of patient’s background. Laboratory findings showed inconstantly hyperleucocytosis and increase in CRP levels. Stool analysis remained the reference laboratory test to detect salmonella. Two major strains of salmonella have been identified: Salmonella enterica subsp. enterica serovar Typhimurium and Enteriditis. Treatments have been essentially symptomatic but 20%of the children (n=34) required antibiotic therapy. A favourable outcome was observed in all the children. Discussion. -Incidence of salmonella infections is increasing in France, particularly in the Languedoc-Roussillon area. Diagnosis of mild forms is generally easy but certain patient background seems to expose to severe forms. Treatment of salmonella infections is mainly symptomatic, only severe or specific cases (young age, immunodepression...) requiring antibiotic treatment. The growing antibiotic use and the increasing of resistance are currently the main problems in the management of salmonella infections. Those infections remain a public health problem in Languedoc-Roussillon.

关键词：沙门菌感染对症处理药物耐药性鼠伤寒沙门菌实验室检测肠炎沙门菌大学医院检查和三联症临床分析

家族性主动脉瘤／夹层动脉瘤合并动脉导管未闭的遗传机制

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上海医学 2007年第S1期30卷 65-65页

作者：朱理敏 Roger VRANCKX Philippe KHAU VAN KIEN Alain lalande Nicolas BOISSET Flavie MATHIEU Mark WEGMAN Luke GLANCY Jean-Marie GASC Francois BRUNOTTE Patrick BRUNEVAL Jean-Eric WOLF Jean-Baptiste MICHEL Xavier JEUNEMAITRE 上海交通大学医学院附属瑞金医院高血压科 200025

目的在16p13-12．1位点20 cm的区域内明确致病基因,了解基因型和临床表型的关系。方法采用双向DNA直接测序法筛选候选基因;运用免疫组化和蛋白免疫印迹法等了解突变基因的表达情况。运用质粒重组及体外表达方法获得野生型和突变型蛋白,... 详细信息

目的在16p13-12．1位点20 cm的区域内明确致病基因,了解基因型和临床表型的关系。方法采用双向DNA直接测序法筛选候选基因;运用免疫组化和蛋白免疫印迹法等了解突变基因的表达情况。运用质粒重组及体外表达方法获得野生型和突变型蛋白,使用免疫共沉淀法了解这两者的结合程度。结果在两个家系中发现编码平滑肌肌球蛋白的MYH11基因突变,所有TAAD和PDA患者均为突变基因携带者。在法国家系中发现两个突变。前者为剪切点供体杂合子突变,导致编码71个氨基酸的第32外显子完全缺失:第2个突变为第37外显子上的1个点突变,导致精氨酸变为谷氨酸。在美国家系中发现1个杂合子突变,为第28外显子上编码24个氨基酸的72个碱基缺失。在法国患者的病变动脉标本上运用蛋白免疫印迹法,发现野生型和突变型肌球蛋白蛋白共存,但有异常免疫组化表现。体外实验显示重组野生型和突变型蛋白不能正常结合。结论MYH11基因突变导致家族性TAAD合并PDA,野生型和突变型平滑肌肌球蛋白不能正常结合,推论杂合子突变导致“Dominant-negative”作用。

关键词：夹层动脉瘤主动脉瘤蛋白免疫印迹法平滑肌肌球蛋白族性突变型野生型免疫共沉淀法基因突变体外表达