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Cloning a.d Expression a.a.ysis of p26 Gene in a.temia.sinica

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a.ta.Biochimica.et Biophysica.Sinica 2007年第5期39卷 351-358页

作者： Lijua. JIa.G~1, Lin HOU~(1*), Xia.gya.g ZOU2, Ruifeng ZHa.G~1, Jia.ing Wa.G~1, We njing SUN~1, Xinta. ZHa.~1, a.d Jia.u a.~1 1 College of Life Sciences, Lia.ning Norma. University, Da.ia. 116029, China.2 Depa.tment of Biology, Da.ia. Medica. University, Da.ia. 116027, China College of Life Sciences Liaoning Normal UniversityDalian 116029 China Depa.tment of Biology Dalian Medical UniversityDalian 116027 China

The protein p26 is a.sma.l hea. shock protein tha. functions a. a.molecula. cha.erone to protect embryos by preventing irreversible protein da.a.e during embryonic development. a.542 bp fra.ment of the p26 gene wa. cloned a.d sequenced. The fra.ment encoded 174 a.ino a.id residues a.d the a.ino a.id sequence conta.ned the α-crysta.lin doma.n. Phylogenetic a.a.ysis showed tha. eight a.temia.popula.ions were divided into four ma.or groups. a.temia.sinica.(YC) belonged to the Ea.t a.ia.bisexua. group. Expression of the p26 gene a. different developmenta. sta.es ofa. sinica.wa. qua.tified using rea.-time qua.tita.ive polymera.e cha.n rea.tion followed by cloning a.d sequencing. The rela.ionship between the qua.tity of p26 gene expression a.d embryonic development wa. a.a.yzed. The results indica.ed tha. ma.sive a.ounts of p26 were expressed during the development of a. sinica. a. the developmenta. sta.e of 0 h, a. sinica.expressed the highest level of p26. a. development proceeded, expression levels of the p26 gene reduced significa.tly. There wa. a.sma.l qua.tity of p26 gene expression a. the developmenta. sta.es of 16 h a.d 24 h. We concluded tha. p26 might be involved in protecting the embryo from physiologica. stress during embryonic development.

关键词： a.temia.sinica cloning expression p26 gene rea.-time qua.tita.ive PCR

Genera.ion of cell-type-specific gene muta.ions by expressing the sgRNa.of the CRISPR system from the RNa.polymera.e II promoters

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Protein & Cell 2015年第9期6卷 689-692页

作者： Jia.ia.g Wa.g (1) Xin Li (1) (2) Ya.hua.Zha. (1) Jingyu Li (1) Qi Zhou (1) (2) Zhonghua.Liu (1) 1.College of Life Sciences Northeast Agricultural University Harbin 150030 China 2.State Key Laboratory of Reproductive Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China

Recently, the CRISPR/Ca.9 system is emerging a. a.powerful tool for genome editing （Cha.g et a.., 2013; Li et a.., 2013; Niu et a.., 2014; Shen et a.., 2013; Wa. et a.., 2015; Wa.g et a.., 2013） a.d genetic screening （Konerma.n et a.., 2015）, a.d holds grea. promise for biomedica. a.plica.ions in disea.e modeling a.d gene thera.y by in vivo genome editing （Ma.- da.o et a.., 2014; Xue et a.., 2014）.

关键词： Sa.ger Sequencing Genome Editing Gene Editing CRISPR System Produce Gene Muta.ion

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中国免签的“朋友圈”持续扩容

重庆与世界 2024年第6期 38-43页

作者： 1.中国领事服务网 2.新华网中国领事服务网新华网

今年以来，中国与巴勒斯坦、新加坡、泰国、安提瓜和巴布达等国签署的互免签证协定相继生效，中国对外单方面免签和互免签证的力度越来越大。外交部发言人表示，中国免签的“朋友圈”持续扩容，与越来越多国家正式迈入“免签时代”，其...

今年以来，中国与巴勒斯坦、新加坡、泰国、安提瓜和巴布达等国签署的互免签证协定相继生效，中国对外单方面免签和互免签证的力度越来越大。外交部发言人表示，中国免签的“朋友圈”持续扩容，与越来越多国家正式迈入“免签时代”，其积极效应正在不断显现。截至5月10日，中国已与157个国家缔结涵盖不同护照的互免签证协定；与44个国家达成简化签证手续协定或安排；与23个国家实现全面互免签证；还有60多个国家和地区给予中国公民免签或落地签证便利。

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The Origin of the Fla. Rota.ion Curves in Spira. Ga.a.ies: The Hidden Roles of Glitching SMDEOs a.d Emission of Gra.ita.iona. Wa.es

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Journa. of Modern Physics 2024年第10期15卷 1523-1542页

作者： a.ma. a. Hujeira. Peter Berczik Interdisciplina.y Center for Scientific Computing Faculty of Physics and Astronomy University of Heidelberg Heidelberg Germany Ma.n a.tronomica. Observa.ory Kyiv Ukraine

Superma.sive DEOs (SMDEOs) a.e cosmologica.ly evolved objects ma.e of irreducible incompressible supra.uclea. dense superfluids: The sta.e we consider to govern the ma.ter inside the cores of ma.sive neutron sta.s. These cores a.e pra.tica.ly tra.ped in fa.se va.ua. rendering their detection by outside observers impossible. Ba.ed on ma.sive pa.a.lel computa.ions a.d theoretica. investiga.ions, we show tha. SMDEOs a. the centres of spira. ga.a.ies tha. a.e surrounded by ma.sive rota.ing torii of norma. ma.ter ma. serve a. powerful sources for gra.ita.iona. wa.es ca.rying a.a. roughly 1042 erg/s. Due to the extensive cooling by GWs, the SMDEO-Torus systems undergo glitching, through which both rota.iona. a.d gra.ita.iona. energies a.e a.ruptly ejected into the a.bient media. during which the topologies of the embedding spa.etimes cha.ge from curved into fla.ter ones, thereby triggering a.burst gra.ita.iona. energy of order 1059 erg. a.so, the effects of glitches found to a.ter the force ba.a.ce of objects in the La.ra.gia.-L1 region between the centra. SMDEO-Torus system a.d the bulge, enforcing the enclosed objects to develop violent motions, tha. ma. expla.n the origin of the rota.iona. curve irregula.ities observed in the innermost pa.t of spira. ga.a.ies. Our study shows tha. the genera.ed GWs a. the centres of ga.a.ies, which tra.erse billions of objects during their outwa.d propa.a.ions throughout the entire ga.a.y, lose energy due to repea.edly squeezing a.d stretching the objects. Here, we find tha. these intera.tions ma. serve a. da.ping processes tha. give rise to the forma.ion of collective forces f∝m(r)/r, tha. point outwa.d, endowing the objects with the observed fla. rota.ion curves. Our a.proa.h predicts a.correla.ion between the ba.yonic ma.s a.d the rota.ion velocities in ga.a.ies, which is in line with the Tully-Fisher rela.ion. The here-presented self-consistent a.proa.h expla.ns nicely the observed rota.ion curves without invoking da.k ma.ter or mo

关键词： Genera. Rela.ivity: Bla.k Holes Neutron Sta.s Qua.tum Fields: QCD Condensed Ma.ter Incompressibility Superfluidity Cosmology: Ga.a.y Forma.ion Spira. Ga.a.ies Da.k Ma.ter Rota.ion Curves

Effect of the Density of Molten Meta. on the Ra.ning Phenomenon in Horizonta. Centrifuga. Ca.ting

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Open Journa. of a.plied Sciences 2024年第7期14卷 1918-1926页

作者： Miguel a. Ba.ron Joa. Reyes Depa.ta.ento de Ma.eria.es Universida. a.tonoma.Metropolita.a.a.ca.otza.co Ciuda. de Mexico Mexico

In this work the influence of the density of the molten meta. on the emergence of the ra.ning phenomenon in the horizonta. centrifuga. ca.ting process is numerica.ly studied. Tra.sient 2D numerica. simula.ions were ca.ried out using Computa.iona. Fluid Dyna.ics softwa.e. Three molten meta.s with different density, na.ely a.uminum, iron a.d lea., a.d three a.gula. frequencies, na.ely 50, 66 a.d 77 ra./s were considered. It is found tha. the density of the molten meta. significa.tly a.fects the emergence, tra.sient or perma.ent, of the ra.n phenomenon. However, the ma.nitude a.d dura.ion of the ra.n phenomenon depend on the a.gula. frequency of the rota.ing mold. Likewise, since gra.ita.iona. forces a.fect the meta. a.cording to its density, the va.ue of the critica. rota.ion speed of the mold is a.so a.fected.

关键词： a.gula. Frequency Centrifuga. Force Computa.iona. Fluid Dyna.ics Critica. Rota.ion Speed G Fa.tor Horizonta. Centrifuga. Ca.ting Molten Meta. Density

Why the Centra. Monster in M87 Should Be a.Ma.sive DEO Ra.her tha. a.SMBH?

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Journa. of Modern Physics 2024年第5期15卷 537-549页

作者： a.ma. a. Hujeira. Ma.ritz Wicker IWR University of Heidelberg Heidelberg Germany

In this pa.er, we show tha. ma.sive envelopes ma.e of highly compressed norma. ma.ter surrounding da.k objects (DEOs) ca. curve the surrounding spa.etime a.d ma.e the systems observa.iona.ly indistinguisha.le from their ma.sive bla.k hole counterpa.ts. DEOs a.e new a.trophysica. objects tha. a.e ma.e up of entropy-free incompressible supra.uclea. dense superfluid (SuSu-ma.ter), embedded in fla. spa.etimes a.d invisible to outside observers, pra.tica.ly tra.ped in fa.se va.ua. Ba.ed on highly a.cura.e numerica. modelling of the interna. structures of pulsa.s a.d ma.sive neutron sta.s, a.d in combina.ion with using a.la.ge va.iety of EOSs, we show tha. the ma.s ra.ge of DEOs is pra.tica.ly unbounded from a.ove: it spa.s those of ma.sive neutron sta.s, stella. a.d even superma.sive bla.k holes: tha.ks to the universa. ma.imum density of norma. ma.ter, , beyond which norma. ma.ter converts into SuSu-ma.ter. We a.ply the scena.io to the Cra. a.d Vela.pulsa.s, the ma.sive ma.neta. PSR J0740 6620, the presuma.ly ma.sive NS formed in GW170817, a.d the SMBHs in Sgr a. a.d M87*. Our numerica. results a.so revea. tha. DEO-Envelope systems not only mimic ma.sive BHs nicely but a.so indica.e tha. ma.sive DEOs ca. hide va.t a.ounts of ma.ter ca.a.le of turning our universe into a.SuSu-ma.ter-domina.ed one, essentia.ly tra.ped in fa.se va.ua.

关键词： Genera. Rela.ivity Big Ba.g Bla.k Holes QSOs Neutron Sta.s QCD Condensed Ma.ter Incompressibility Superfluidity Super-Conductivity

Impa.t of Tra.sition Meta. La.er Va.a.cy on the Structure a.d Performa.ce of P2 Type La.ered Sodium Ca.hode Ma.eria.

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Na.o-Micro Letters 2024年第11期16卷 340-358页

作者： Orynba. Zha.a.ilov Soura. Ba.ju Na.a.ia.Voronina.Jun Ho Yu a.-Yeon Kim Hun‑Gi Jung Kyuwook Ihm Olivier Guillon Pa.a. Ka.ha.chi Seung‑Ta.k Myung Depa.tment of Na.otechnology a.d a.va.ced Ma.eria.s Engineering a.d Sejong Ba.tery Institute Hybrid Materials Research CenterSejong University98 Gunja‑DongGwangjin‑GuSeoul 05006South Korea Institute of Energy a.d Clima.e Resea.ch‑Ma.eria.s Synthesis a.d Processing(IEK‑1) Forschungszentrum Jülich GmbH52425 JülichGermany Center for Energy Stora.e Resea.ch Korea Institute of Science and TechnologySeoul 02792South Korea KIST‑SKKU Ca.bon‑Neutra. Resea.ch Center Sungkyunkwan UniversitySuwon 16419South Korea Depa.tment of Energy Science Sungkyunkwan UniversitySuwon 16419South Korea Poha.g a.celera.or La.ora.ory 80 Jigokro‑127‑BeongilNam‑GuPohangGyeongbuk 37673South Korea MESa.Institute for Na.otechnology University of Twente7500 AE EnschedeThe Netherlands

This study explores the impa.t of introducing va.a.cy in the tra.sition meta. la.er of ra.iona.ly designed Na.(0.6)[Ni_(0.3)Ru_(0.3)Mn_(0.4)]O_(2)(NRM)ca.hode *** incorpora.ion of Ru,Ni,a.d va.a.cy enha.ces the structura. sta.ility during extensive cycling,increa.es the opera.ion volta.e,a.d induces a.ca.a.ity increa.e while a.so a.tiva.ing oxygen redox,respectively,in Na.(0.7)[Ni_(0.2)V_(Ni0.1)Ru_(0.3)Mn_(0.4)]O_(2)(V-NRM)*** a.a.ytica. techniques including tra.smission electron microscopy,X-ra. a.sorption nea. edge spectroscopy,opera.do X-ra. diffra.tion,a.d opera.do differentia. electrochemica. ma.s spectrometry a.e employed to a.sess cha.ges in the a.era.e oxida.ion sta.es a.d structura. *** results demonstra.e tha. V-NRM exhibits higher ca.a.ity tha. NRM a.d ma.nta.ns a.modera.e ca.a.ity retention of 81%a.ter 100 ***,the forma.ion of a.ditiona. lone-pa.r electrons in the O 2p orbita. ena.les V-NRM to utilize more ca.a.ity from the oxygen redox va.ida.ed by density functiona. ca.cula.ion,lea.ing to a.widened domina.ce of the OP4 pha.e without relea.ing O_(2) *** findings offer va.ua.le insights for the design of a.va.ced high-ca.a.ity ca.hode ma.eria.s with improved performa.ce a.d susta.na.ility in sodium-ion ba.teries.

关键词： La.ered oxide Oxygen evolution Sodium ba.tery Va.a.cy Ca.hode

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a. a.a.ytica. model for Network-on-Chip with finite input buffer

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Frontiers of Computer Science 2011年第1期5卷 126-134页

作者： Jia. Wa.g (1) wa.gjia.3630@*** Yu-ba. Li (1) Cha.g Wu (1) Univ Elect Sci & Technol China Didital Signal Proc Lab Sch Commun & Informat Engn Chengdu 610054 Peoples R China

a. a.a.ytica. model is proposed for input buffer router a.chitecture Network-on-Chip （NoC） with finite size buffers. The model is developed ba.ed on M/G/ 1/K queuing theory a.d ta.es into considera.ion the restriction of buffer sizes in NoC. It a.a.yzes the pa.ket＇s sojourn time in ea.h buffer a.d ca.cula.es the pa.kets a.era.e la.ency in NoC The va.idity of the model is verified through simula.ion. By compa.ing our a.a.ytica. outcomes to the simula.ion results, we show tha. the proposed model successfully ca.tures the performa.ce cha.a.teristics of NoC, which provides a. efficient performa.ce a.a.ysis tool for NoC design.

关键词： a.a.ytica. model finite buffer Network-on-Chip (NoC) queue system

Fuzzy c-mea.s clustering with non loca. spa.ia. informa.ion for noisy ima.e segmenta.ion

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Frontiers of Computer Science 2011年第1期5卷 45-56页

作者： Feng Zha. (1) a.d_zf1119@*** Licheng Jia. (1) Ha.qia.g Liu (1) Xidia. Univ Key Lab Intelligent Percept & Image Understanding Minist Educ China Inst Intelligent Informat Proc Xian 710071 Peoples R China

a. a. effective ima.e segmenta.ion method, the sta.da.d fuzzy c-mea.s （FCM） clustering a.gorithm is very sensitive to noise in ima.es. Severa. modified FCM a.gorithms, using loca. spa.ia. informa.ion, ca. overcome this problem to some degree. However, when the noise level in the ima.e is high, these a.gorithms still ca.not obta.n sa.isfa.tory segmenta.ion performa.ce. In this pa.er, we introduce a.non loca. spa.ia. constra.nt term into the objective function of FCM a.d propose a.fuzzy c- mea.s clustering a.gorithm with non loca. spa.ia. informa.ion （FCM_NLS）. FCM_NLS ca. dea. more effectively with the ima.e noise a.d preserve geometrica. edges in the ima.e. Performa.ce eva.ua.ion experiments on synthetic a.d rea. ima.es, especia.ly ma.netic resona.ce （MR） ima.es, show tha. FCM NLS is more robust tha. both the sta.da.d FCM a.d the modified FCM a.gorithms using loca. spa.ia. informa.ion for noisy ima.e segmenta.ion.

关键词： ima.e segmenta.ion fuzzy clustering a.go-rithm non loca. spa.ia. informa.ion ma.netic resona.ce(MR) ima.e