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Multifunctional zinc ion doped sol-gel derived mesoporous bioactive glass nanoparticles for biomedical applications

Bioactive Materials 2019年第1期4卷 312-321页

作者： zuzana neščáková Kai Zheng Liliana Liverani Qaisar Nawaz Dagmar Galusková Hana Kaňková Martin Michálek Dušan Galusek Aldo R.Boccaccini Dept.of Biomaterials FunGlass-Centre for Functional and Surface Functionalized GlassAlexander Dubček University of TrenčínSlovakia Central Laboratories FunGlass-Centre for Functional and Surface Functionalized GlassAlexander Dubček University of TrenčínSlovakia Institute of Biomaterials Department of Materials Science and EngineeringUniversity of ErlangenNurembergGermany

Mesoporous bioactive glasses have been widely investigated for applications in bone tissue regeneration and,more recently,in soft tissue repair and wound *** this study we produced mesoporous bioactive glass nanoparticles(MBGNs)based on the SiO2-CaO *** the intention of adding subsidiary biological function,MBGNs were doped with Zn2+***-MBGNs with 8 mol%ZnO content were synthesized via microemulsion assisted sol-gel *** synthesized particles were homogeneous in shape and *** exhibited spherical shape,good dispersity,and a size of 130±10 *** addition of zinc precursors did not affect the morphology of particles,while their specific surface area increased in comparison to *** presence of Zn2+ions inhibited the formation of hydroxycarbonate apatite(HCAp)on the particles after immersion in simulated body fluid(SBF).No formation of HCAp crystals on the surface of Zn-MBGNs could be observed after 14 days of ***,powders containing relatively high amount of zinc released Zn2+ions in low concentration(0.6-1.2 mg L^−1)but in a sustained *** releasing feature enables Zn-MBGNs to avoid potentially toxic levels of Zn2+ions,indeed Zn-MBGNs were seen to improve the differentiation of osteoblast-like cells(MG-63).Additionally,Zn-MBGNs showed higher ability to adsorb proteins in comparison to MBGNs,which could indicate a favourable later attachment of *** to their advantageous morphological and physiochemical properties,Zn-MBGNs show great potential as bioactive fillers or drug delivery systems in a variety of applications including bone regeneration and wound healing.

关键词： Bioactive particles Sol-gel synthesis Zinc Bioactivity Ion release Bioactive glass

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Species traits and shoot-root biomass allocation in 20 dry-grassland species

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Journal of Plant Ecology 2018年第2期11卷 273-285页

作者： Iveta Husáková Jacob Weiner zuzana Münzbergová Department of Botany Faculty of ScienceCharles UniversityBenátská2CZ-12801 PragueCzech Republic Institute of Botany Academy of Sciences of the Czech RepublicZámek 1CZ-25243 PrůhoniceCzech Republic Department of Plant and Environmental Sciences University of CopenhagenThorvaldsensvej 40DK-1871 FrederiksbergDenmark

Aims A plant has a limited amount of resources at any time and it allo-cates them to different *** spite of the large number of previous studies on allocation patterns within single species,knowledge of general patterns in species allocation is still very *** is because each study was done in different condi-tions using different methodology,making generalization *** investigate intraspecific above-versus below-ground biomass allocation among individuals across a spectrum of dry-grassland plant species at two different developmental stages and ask whether allocation is age-and species specific,and whether differences among species can be explained by their life-history traits and *** We collected data on above-and below-ground biomass of seedlings and adult plants of 20 species from a common garden *** analysed data on shoot-root biomass allocation allometrically and studied the relationship between the allometric exponents(slopes on log-log scale),species life-history traits and phylogenetic *** Findings We found isometric as well as allometric patterns of biomass alloca-tion in the studied *** and adult individuals of more than half of the species differed in their above-versus below-ground biomass allometric *** and adult individuals of the remaining species differed in their allometric coefficients(inter-cepts).Annual species generally allocated proportionally more to above-than below-ground biomass as seedlings than as adults,whereas perennial species showed the opposite *** life-history traits,such as plant life span,age of first flowering,month in which the species begin flowering and specific leaf area were much more important in explaining differences in shoot-root allometry among species than were phylogenetic *** suggests that allocation patterns vary greatly among closely related species but can be predicted based on species life-hi

关键词： above-ground biomass below-ground biomass allometry isometry seedling adult plant life-history traits phylogenetic relatedness root-shoot ratio

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Automated tree detection and crown delineation using airborne laser scanner data in heterogeneous East-Central Europe forest with different species mix

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Journal of Forestry Research 2017年第5期28卷 1049-1059页

作者： Róbert Smreek Ivan Sakov zuzana Michnová Ján Tuek Department of Forest Management and Geodesy Technical University in Zvolen Department of Forest Policy Economics and Forest ManagementNational Forest Centre-Forest Research Institute Zvolen

Identifying tree locations is a basic step in the derivation of other tree parameters using remote sensing techniques, particularly when using airborne laser scanning. There are several techniques for identifying tree positions. In this paper, we present a raster-based method for determining tree position and delineating crown coverage. We collected data from nine research plots that supported different mixes of species. We applied a raster-based method to raster layers with six different spatial resolutions and used terrestrial measurement data as reference data. Tree identification at a spatial resolution of 1.5 m was demonstrated to be the most accurate, with an average identification ratio (IR) of 95% and average detection ratio of 68% being observed. At a higher spatial resolution of 0.5 m, IR was overestimated by more than 600%. At a lower spatial resolution of 3 m, IR was underestimated at less than 44% of terrestrial measurements. The inventory process was timed to enable evaluation of the time efficiency of automatic methods.

关键词： Airborne laser scanning East-Central Europe Individual tree Mixed forest Tree crown delineation

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How can stream bank erosion be predicted on small water courses?Veri?cation of BANCS model on the Kubrica watershed

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International Journal of Sediment Research 2021年第3期36卷 419-429页

作者： zuzana Allmanová Máaria Vl?kováa Martin Jankovsky Michal Allman Jáan Mergani? Department of Forest Harvesting Logistics and Ameliorations Faculty of Forestry Technical University in Zvolen Department of Forestry Technology and Constructions Faculty of Forestry and Wood Sciences Czech University of Life Sciences Prague

The current paper deals with the evaluation of the BANCS erosion prediction model and its two componentsethe Bank Erosion Hazard Index（BEHI） and near-Bank Stress（NBS） indices. To construct the erosion prediction curves, 18 experimental sections were established on the Kubrica Stream, district of Trencín, Slovakia. Each section was assessed through the NBS index and BEHI index and real annual bank erosion was measured using erosion toe pins. Subsequently, the relations between the BEHI and real annual bank erosion was assessed through regression and correlation analyses. The relation proved to be moderately strong, with the correlation coefficient（R） reaching 0.47. Further, the relation between the NBS index and real annual bank erosion was evaluated, which was also moderately strong, with R= *** on the measured data, two erosion prediction curves were constructed, the first for moderate BEHI, with R= 0.69 and coefficient of determination（R2） of 0.47 and the second for high BEHI with R=0.74 and R2= 0.55. The prediction curves were based on data from one year of measurements and can, therefore, be used only for discharges that occurred within that year and in the region where the model was developed. In the current case, according to runoff Curve Numbers（CN）, the real culmination discharge was Q ? 1.88 m3/s, which is roughly equivalent to 1.5-year recurrence interval flow（Q1.5）.

关键词： Bank erosion Bank assessment for non-point source consequences of sediment (BANCS) Bank erosion hazard index near-bank stress index Erosion prediction curves

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new species in Aspergillus section Fumigati from reclamation sites in Wyoming (U.S.A.) and revision of A. viridinutans complex

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Fungal Diversity 2014年第1期64卷 253-274页

作者： Alena Nováková Vit Hubka zuzana Dudová Tetsuhiro Matsuzawa Alena Kubátová Takashi Yaguchi Miroslav Kolařík Biology Centre AS CR v.v.i.Institute of Soil BiologyNa Sádkách 737005ČeskéBudějoviceCzech Republic Laboratory of Physiology and Genetics of Fungi Institute of Microbiology AS CRv.v.iVídeňská108314220 Prague 4Czech Republic Department of Botany Faculty of ScienceCharles University in PragueBenátská212801 Prague 2Czech Republic Medical Mycology Research Center Chiba University1-8-1InohanaChuo-kuChiba 260-8673Japan

The Aspergillus viridinutans complex includes morphologically similar,soil-inhabiting *** its species boundaries have not been fully defined,many isolates from the complex have been isolated as opportunistic human and animal *** the present study,these species were dominant in spoil sites subjected to various types of reclamation management after coal *** species were characterised using two different PCR-fingerprinting methods,sequence data from the β-tubulin(benA)and calmodulin(caM)genes,macro-and micromorphology(optical and scanning electron microscopy),maximum growth temperatures and mating *** addition,RNA polymerase II gene(RPB2),actin(act1)and ITS sequences were deposited for the ex-type isolates of newly described *** mating experiment results,phylogenetic analyses and ascospore morphology suggested the presence of five species in the *** *** aureolus(*** aureola)was the only homothallic *** species,***,***(***)and *** ***.,were heterothallic and their morphologically distinguishable teleomorph was induced by systematic mating *** viridinutans *** to be a very rare species and was represented only by the ex-type isolate in which the MAT1-1 locus was *** viridinutans and *** were typified in accordance with the rules of the new botanical *** species outside the *** complex isolated from the reclamation sites were *** and *** as well as two new sister species,*** *** *** *** were closely related each to other and to *** new species are phylogenetically distant from all anamorphic species and resemble ***,*** and *** in micromorphology and are distinguishable from each other by the slower growth of *** on all tested ***,no isolate from the

关键词： Aspergillus fumigatus Heterothallic species MAT locus neosartorya udagawae PCR fingerprinting Soil fungi

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多壁纳米碳管对磷酸铁锂正极材料热稳定性及表面形貌的影响(英文)

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新型炭材料 2013年第1期28卷 1-7页

作者： Mária Filkusová Andrea Fedorková Renáta Oriňáková Andrej Oriňák zuzana Nováková Lenka Skantárová Department of Physical and Theoretical Chemistry Faculty of Science Comenius University Mlynská|Dolina SK-842 15 Bratislava 4 Slovak Republic Department of Physical Chemistry Faculty of Science P.J. afárik University Moyzesova 11 SK-04154 Koice Slovak Republic Department of Electrical and Electronic Technology Faculty of Electrical Engineering and Communication Brno University of Technology Czech Republic Department of Analytical Chemistry Faculty of Science Comenius University Mlynská|Dolina SK-842 15 Bratislava 4 Slovak Republic

合成了一种新型的LiFePO4/多壁纳米碳管(MWCNTs)复合正极材料。通过MWCNTs和PPy-PEG粘结剂有机地在LiFePO4颗粒间构成三维网络导电结构,并借助SEM、TGA和高分辨裂解气相色谱,研究了LiFePO4-MWCNT正极材料的表面形貌和热稳定性。结果表明... 详细信息

合成了一种新型的LiFePO4/多壁纳米碳管(MWCNTs)复合正极材料。通过MWCNTs和PPy-PEG粘结剂有机地在LiFePO4颗粒间构成三维网络导电结构,并借助SEM、TGA和高分辨裂解气相色谱,研究了LiFePO4-MWCNT正极材料的表面形貌和热稳定性。结果表明,500℃下该材料的热分解程度非常小,说明MWCNTs和PPy-PEG的复合是提高LiFePO4正极材料的热稳定性和改善表面形貌的有效途径。

关键词：多壁纳米碳管多吡咯磷酸铁锂锂离子电池

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Making the Genotypic Variation Visible:Hyperspectral Phenotyping in Scots Pine Seedlings

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Plant Phenomics 2023年第4期5卷 790-804页

作者： Jan Stejskal Jaroslav Čepl Eva neuwirthová Olusegun Olaitan Akinyemi Jiří Chuchlík Daniel Provazník Markku Keinänen Petya Campbell Jana Albrechtová Milan Lstiburek zuzana Lhotáková Department of Genetics and Physiology of Forest Trees Faculty of Forestry and Wood SciencesCzech University of Life Sciences PraguePragueCzech Republic Department of Environmental and Biological Sciences University of Eastern FinlandJoensuuFinland Department of Experimental Plant Biology Charles UniversityPragueCzech Republic Center for Photonic Sciences University of Eastern FinlandJoensuuFinland Department of Geography and Environmental Sciences University of Maryland Baltimore CountyBaltimoreMDUSA Biospheric Sciences Laboratory NASA Goddard Space Flight CenterGreenbeltMDUSA

Hyperspectral reflectance contains valuable information about leaf functional traits,which can indicate a plant's physiological ***,using hyperspectral reflectance for high-throughput phenotyping of foliar traits could be a powerful tool for tree breeders and nursery practitioners to distinguish and select seedlings with desired adaptation potential to local *** evaluated the use of 2 nondestructive methods(i.e.,leaf and proximal/canopy)measuring hyperspectral reflectance in the 350-to 2,500-nm range for phenotyping on 1,788 individual Scots pine seedlings belonging to lowland and upland ecotypes of 3 different local populations from the Czech ***-level measurements were collected using a spectroradiometer and a contact probe with an internal light source to measure the biconical reflectance factor of a sample of needles placed on a black background in the contact probe field of *** proximal canopy measurements were collected under natural solar light,using the same spectroradiometer with fiber optical cable to collect data on individual seedlings'hemispherical conical reflectance *** latter method was highly susceptible to changes in incoming *** spectral datasets showed statistically significant differences among Scots pine populations in the whole spectral ***,using random forest and support vector machine learning algorithms,the proximal data obtained from the top of the seedlings offered up to 83%accuracy in predicting 3 different Scots pine *** conclude that both approaches are viable for hyperspectral phenotyping to disentangle the phenotypic and the underlying genetic variation within Scots pine seedlings.

关键词： Scots forest reflectance

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Effect of soil properties and sample preparation on extractable and soluble Pb and Cd fractions in soils

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Agricultural Sciences 2010年第3期1卷 119-130页

作者： Jirina Száková Daniela Miholová Pavel Tlustos Ivana Sestáková zuzana Frková Czech University of Life Sciences Prague Faculty of AgrobiologyFood and Natural ResourcesPragueCzech Republic J.HeyrovskýInstitute of Physical Chemistry of ASCR PragueCzech Republic

The effect of soil extraction procedures and/or sample pretreatment (drying, freezing of the soil sample) on the extractability of cadmium and lead was tested in a model experiment, with an employment of optical emission and atomic ab- sorption spectrometry methods. In the first part, 6 extraction procedures were compared: 2 mol l-1 HNO3, 0.43 mol l-1 CH3COOH, 0.05 mol l-1 EDTA, Mehlich III extraction procedure (0.2 mol l-1 CH3COOH + 0.25 mol l-1 NH4NO3 + 0.013 mol.l-1 HNO3 + 0.015 mol.l-1 NH4F + 0.001 mol.l-1 EDTA), 0.01 mol.l-1 CaCl2, and deionised water. Addi-tionally, two methods of soil solution sampling were compared, and the centrifugation of satu-rated soil and the use of suction cups and dif-ferential pulse anodic stripping voltametry was applied to assess free and complexed metals portions. The results showed that different soil sample extraction methods and/or sample pre-treatments including soil solution sampling can lead to different absolute values of mobile cadmium and lead content in soils. However, the interpretation of the data can lead to similar conclusions as are apparent from the compari- son of the soil solution sampling methods where fairly good correlation was observed (for Cd r = 0.76, and for Pb r = 0.74). The ambiguous results were reported for voltammetric determinations of free and complex portions of Cd and Pb where a different behavior was observed for water extracts of soil and soil solution obtained using suction cups. Moreover, a changing extent of lead complexation was determined with prolonged storage of the samples. The results confirmed that soil and/or soil solution sampling under immediate soil conditions and limitations of pre-extraction operations are necessary.

关键词： Lead Cadmium Contaminated Soils Extractability Soil Solution Speciation

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Testing of a new Collagenase Blend for Pancreatic Islet Isolation Produced by Clostridium histolyticum

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Advances in Bioscience and Biotechnology 2018年第1期9卷 26-35页

作者： zuzana Berková Frantisek Saudek Ivan Leontovyc Martin Benesík Dana Stveráková Laboratory of Pancreatic Islets Institute for Clinical and Experimental MedicinePragueCzech Republic Department of Diabetes Institute for Clinical and Experimental MedicinePragueCzech Republic MB Pharma Ltd. PragueCzech Republic

Clostridium histolyticum is used for production of several proteolytic enzymes such as elastase, neutral proteases, clostripain and in particular collagenase. Besides industrial applications, collagenase has been indispensable for medical purposes including isolation of pancreatic islets for diabetes treatment. The aim of this study was to optimize the method for production and partial purification of a new collagenase blend and to test its suitability for successful pancreatic islets isolation in a rat model. Bacterial strain of C. histolyticum was sequenced for presence of the collagenase genes. Different fermentation conditions were tested and the process of collagenase extraction was modified and optimized. Samples of collagenases were taken for western blot detection, activity assessment, and ability for dissociation of pancreatic tissue. Findings indicate that concentrated trypton growth medium with pepton was the most suitable for Clostridium growth and collagenase production. Whole genome sequencing revealed two genes for collagenase and also gene for clostripain. Western blot specific detection helped to select useful production modifications. Following these modifications was also improved the yield, morphology and in vitro function of intact pancreatic islets which were finally comparable or better than those achieved using standard blends of collagenase. The results support the use of the new collagenase blend for islet isolation giving thus the opportunity to choose an alternative product. Our next steps would lead to further enzyme purification through scaling up of the production method for a wider use.

关键词： Clostridium histolyticum Fermentation Collagenase Pancreatic Islets Islet Isolation

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Assessing impact of land use and climate change on regulating ecosystem services in the Czech Republic

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Ecosystem Health and Sustainability 2016年第3期2卷 1-13页

作者： Eliška Krkoška Lorencová zuzana V.Harmáčková Lucie Landová Adam Pártl David Vačkář Global Change Research Institute The Czech Academy of SciencesBělidla 986/4a60300BrnoCzech Republic Faculty of Humanities Charles University in PragueU Kříže 815000Prague 5-JinoniceCzech Republic Environment Center Charles University in PragueJ.Martího 2/40716200Prague 6Czech Republic

Ecosystem services,defined as benefits provided by ecosystem functioning to society,are essential to human *** to global environmental change and related anthropogenic drivers,ecosystems are often degraded,which hinders the delivery of ecosystem *** study aims to quantify the impacts of land use and climate change on two regulating ecosystem services-carbon sequestration and water purification in terms of nitrogen retention in the Czech *** employing approaches of scenarios and modeling,we illustrate current and potential future status of these ecosystem *** results show that among the ecosystem change drivers,one of the dominant domestic land-use change of ALARM BAMBU(Business-As Might Be Usual)scenario is change of arable land and grassland to forest area that increases by 4.5%in 2080 compared to *** results of ecosystem service modeling based on BAMBU scenario for the years 2050 and 2080 indicate that the highest yearly carbon sequestration rate occurred in 2000-2050,reaching 640 GgC·yr^(−1),2000-2080 shows decline in this regulating service by 16%.Average nitro-gen leaching to water streams reached 0.75 kgN·ha^(−1)·yr^(−1)for BAMBU in 2050 scenario and 0.80 kgN·ha^(−1)·yr^(−1)for BAMBU in 2080 scenario as a result of decreasing nitrogen load,which suggested a decrease in nitrogen pollution compared to *** ecosystem services have not been extensively mainstreamed into research and policies in eastern European countries,we aim to contribute to improvement of knowledge on current status and potential future pathways of the provision of regulating ecosystem services in the Czech Republic.

关键词： Czech Republic drivers ecosystem services InVEST modeling scenarios Special Feature:Ecosystem Management in Transition in Central and Eastern Europe

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