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Effect of Probe Lifting Height in Jumping Mode AFM for Living Cell Imaging

Nanomanufacturing and Metrology 2023年第3期6卷 51-59页

作者： Can Cheng Xingyue wang Jianjun Dong zuobin wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun130022China Centre for Opto/Bio-Nano Measurement and Manufacturing Zhongshan Institute of Changchun University of Science and TechnologyZhongshan528437China Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun130022China JR3CN&IRAC University of BedfordshireLutonLU13JUUK

Atomic force microscopy(AFM)is one of the effective methods for imaging the morphological and physical properties of living cells in a near-physiological environment.However,several problems caused by the adhesion of living cells and extension of the cell membranes seriously affect the image quality during living cell imaging,hindering the study of living cells.In this work,jumping mode AFM imaging was used to image living cells at varied probe lifting heights to meet image quality requirements,and image quality related to the probe lifting height is discussed in detail.The jumping mode was divided into three parts based on the varying heights of the lifted probe,namely near-contact mode,half-jumping mode,and full-jumping mode,and the causes of their imaging drawbacks were analyzed.At an appropriate lifting height,the probe can be completely free from the influence of cell adhesion and self-excited oscillation,thus avoiding the occurrence of“trail”phenomena and invalid points in the imaging of living cells and improving the image quality.Additionally,this work provides a new approach to calculating the lateral force through the adhesion of trace and retrace scanning at a low height,which is important for studying the extension characteristics of the cell membrane.

关键词： Atomic force microscopy Jumping mode Lifting height Living cell imaging

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HG-Induced sEVs Mediate Biomechanics of HK-2 Cells

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Nanomanufacturing and Metrology 2023年第4期6卷 81-91页

作者： Fan Yang Jiajia wang Tuoyu Ju Shuwei wang Kaige Qu Zhengxun Song Yujuan Chen zuobin wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun 130022China Centre for Opto/Bio-Nano Measurement and Manufacturing Zhongshan Institute of Changchun University of Science and TechnologyZhongshan 528437China Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun 130022China Afliated Hospital Jilin Medical UniversityJilin 132013China JR3CN&IRAC University of BedfordshireLuton LU13JUUK

Small extracellular vesicles (sEVs) participate in the pathological progression of high glucose (HG)-induced kidney injury, which is closely related to diabetic nephropathy. How sEVs specifically mediate the cell biomechanics underlying HG injury is unclear. Herein, we utilized a versatile atomic force microscope to determine the contributions of sEVs in HG-induced cellular injury. The sEVs extracted from the culture medium of human proximal tubule kidney (HK-2) cells treated by HG for 72 h (HG-induced sEVs) were verified and analyzed by multiple techniques, and the results indicated the effective production and the effect of dehydration on the shape of HG-induced sEVs. Further investigation on the morphologies of HK-2 cells treated by HG-induced sEVs showed that the surface roughness of the HK-2 cells increased, and their pseudopodia transitioned from lamellipodia to filopodia, with almost doubled mean pseudopodia length. Quantitative analysis of the mechanical responses of the cells revealed that the mean Young’s modulus increased by 26.2%, and the mean adhesion decreased by 36.8%. The indirect mediation of cellular biomechanics guided by HG-induced sEVs was evaluated by comparing it with previously studied direct HG injury. The HG-induced sEVs caused a greater reduction in cell adhesion and an increase in Young’s modulus compared with direct HG stimulation. This work suggested the ability of HG-induced sEVs to elicit specific biomechanical responses during HG injury, advancing the understanding of the injury mechanism caused by HG. The comparison of the cellular biomechanics between direct and indirect HG stimulations through HG-induced sEVs can be beneficial for the diagnosis and treatment of kidney injury.

关键词： AFM Cellular biomechanics HG-induced sEVs HG HK-2 cells

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Biomimetic Superlyophobic Metallic Surfaces: Focusing on Their Fabrication and Applications

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Journal of Bionic Engineering 2020年第1期17卷 1-33页

作者： Zhongxu Lian Jinkai Xu zuobin wang Huadong Yu Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing Changchun University of Science and TechnologyChangchun 130022China International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun 130022China

Metals are indispensable engineered materials for day-to-day life.Researches focused on metallic surfaces with superlyophobicity(superhydrophobicity,superoleophobicity,underwater superoleophobicity and slippery characteristic)have attracted much attention recently.Nature is a magician that gives each organic life a unique advantage.Researchers have created a large number of biomimetic superlyophobic metallic surfaces through various approaches.These biomimetic superlyophobic metallic surfaces exhibit advantages in many applications,such as self-cleaning,corrosion resistance,anti-icing,and drag reduction.In this review,the specific fabrication and applications of biomimetic superlyophobic metallic surfaces were reported.The remaining challenges and future outlook of biomimetic superlyophobic metallic surfaces were preliminarily analyzed.It is hoped that the review will be essential for broadening the scope of potential applications of metals and providing a powerful reference for future research on metal-based advanced functional materials.

关键词： biomimetic metallic surfaces superhydrophobicity superoleophobicity underwater superoleophobicity slippery characteristic

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Nanomaterial-based flexible sensors for metaverse and virtual reality applications

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International Journal of Extreme Manufacturing 2023年第3期5卷 407-439页

作者： Jianfei wang Jiao Suo Zhengxun Song Wen Jung Li zuobin wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyJilin130022People’s Republic of China Department of Mechanical Engineering City University of Hong KongKowloonHong Kong Special Administrative Region of ChinaPeople’s Republic of China

Nanomaterial-based flexible sensors(NMFSs)can be tightly attached to the human skin or integrated with clothing to monitor human physiological information,provide medical data,or explore metaverse spaces.Nanomaterials have been widely incorporated into flexible sensors due to their facile processing,material compatibility,and unique properties.This review highlights the recent advancements in NMFSs involving various nanomaterial frameworks such as nanoparticles,nanowires,and nanofilms.Different triggering interaction interfaces between NMFSs and metaverse/virtual reality(VR)applications,e.***.skin-mechanics-triggered,temperature-triggered,magnetically triggered,and neural-triggered interfaces,are discussed.In the context of interfacing physical and virtual worlds,machine learning(ML)has emerged as a promising tool for processing sensor data for controlling avatars in metaverse/VR worlds,and many ML algorithms have been proposed for virtual interaction technologies.This paper discusses the advantages,disadvantages,and prospects of NMFSs in metaverse/VR applications.

关键词： flexible sensors metaverse virtual reality human-computer interaction machine learning

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Bioelectrochemical acidolysis of magnesia to induce struvite crystallization for recovering phosphorus from aqueous solution

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Journal of Environmental Sciences 2019年第11期31卷 119-128页

作者： zuobin wang Jiao Zhang Xiao Guan Lu She Pengyu Xiang Siqing Xia Zhiqiang Zhang Key Laboratory of Yangtze River Water Environment Ministry of Education State Key Laboratory of Pollution Control and Resource ReuseCollege of Environmental Science and Engineering Tongji University Shanghai 200092 China Shanghai Institute of Pollution Control and Ecological Security Shanghai 200092 China School of Civil and Transportation Engineering Shanghai Urban Construction Vocational College Shanghai 200432 China Zhejiang Weiming Environment Protection Co. Ltd. Wenzhou 325000 China

A novel struvite crystallization method induced by bioelectrochemical acidolysis of magnesia (MgO) was investigated to recover phosphorus (P) from aqueous solution using a dual-chamber microbial electrolysis cell (DMEC).Magnesium ion (Mg2+) in the anolyte was firstly confirmed to automatically migrate from the anode chamber to the cathode chamber,and then react with ammonium (NH4+) and phosphate (PO34-) in the catholyte to form struvite.Recovery efficiency of 17.8%-60.2% was obtained with the various N/P ratios in the catholyte.When MgO (low solubility under alkali conditions) was added into the anolyte,the bioelectrochemical acidolysis of MgO naturally took place and the released Mg2+ induced struvite crystallization in the cathode chamber for P recovery likewise.Besides,there was a strong linear positive correlation between the recovery efficiency and the MgO dosage (R2 =0.935),applied voltage (R2 =0.969) and N/P ratio (R2 =0.905).Increasing the applied voltage was found to enhance the P recovery via promoting the MgO acidolysis and the released Mg2+ migration,while increasing the N/P ratio in the catholyte enhanced the P recovery via promoting the struvite crystallization.Moreover,the electrochemical performance of the system was promoted due to more stable anolyte pH and lower pH gradient between the two chambers.Current density was promoted by 10%,while the COD removal efficiency was improved from 78.2% to 91.8% in the anode chamber.

关键词： Phosphorus Struvite Microbial electrolysis cell Magnesia Acidolysis Migration

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仿生鲨鱼皮复合微纳减风阻结构的仿真与制备

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实验流体力学 2024年第2期38卷 107-114页

作者：徐征刘日王天昊迟振东王作斌李理长春理工大学国家纳米操纵与制造国际联合研究中心长春130022

仿生学与减阻技术的结合,为减阻开辟了重要的研究方向,在航空航天领域有着潜在的发展与应用前景。为提高降低风阻效果,本文对复合微纳减风阻结构进行了研究,基于仿生学原理,采用CFD仿真及激光微纳制造技术,建立了减阻结构组合模型,并在... 详细信息

仿生学与减阻技术的结合,为减阻开辟了重要的研究方向,在航空航天领域有着潜在的发展与应用前景。为提高降低风阻效果,本文对复合微纳减风阻结构进行了研究,基于仿生学原理,采用CFD仿真及激光微纳制造技术,建立了减阻结构组合模型,并在飞行器的大气传感器半球头体模型表面制造仿生鲨鱼皮复合微纳结构,即在仿生鲨鱼皮鳞片结构的基础上,通过激光干涉扫描二级微沟槽,以进一步提升减阻效果。采用CFD仿真与风洞实验相结合的方式,对减阻机理进行理论分析,完成了复合结构的微纳制造,减阻率最高可达10.3%。

关键词：鲨鱼皮减阻风洞实验 CFD流场仿真微纳结构鳞片仿生

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Response of MG63 Osteoblast Cells to Surface Modification of Ti-6Al-4V Implant Alloy by Laser Interference Lithography

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Journal of Bionic Engineering 2017年第3期14卷 448-458页

作者： Qi Liu Wenjun Li Liang Cao Jiajia wang Yingmin Qu Xinyue wang Rongxian Qiu Xu Di zuobin wang Bojian Liang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and Technology Changchun 130022 China Department of Orthopaedic Surgery China-Japan Union Hospital of Jilin University Changchun 130033 China

The response of human osteoblast-like osteosarcoma cells （MG63） to surface modification of Ti-6Al-4V implant alloy was investigated by Laser Interference Lithography （LIL）. In this work, laser interference lithography was employed to fabricate the microstructures of grooves, dots and dimples onto the surfaces of Ti-6Al-4V samples. Two and three beam LIL systems were developed to carry out the experiments. The laser treatment resulted in the increases of the roughness and the contact angle of water on the implant alloy surfaces. The proliferation of osteoblasts was analyzed by MTT （3-（4,5-dirnethyl- 2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide） assay for the time periods of 4 hours, 2 days, 3 days, and 6 days. The MTT test results demonstrated that the laser treatment surfaces had a positive impact on the proliferation of os- teoblast cells after 24 hours. The alloy surface morphology and the morphological changes of MG63 cells cultured on the laser textured Ti-6Al-4V surface were observed by Scanning Electron Microscope （SEM）. The SEM results indicated that the os- teoblast cells were aligned on grooved surfaces and they were prolonged with the structures. Enzymatic detachment results showed that the 20 μm grooved structures provided the better cell adhesion to the textured Ti-6Al-4V surfaces.

关键词： Ti-6Al-4V implant alloy laser interference lithography surface modification surface properties cell-substrate interaction

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微纳结构表面防冰研究进展

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表面技术 2024年第11期53卷 100-116页

作者：周嘉鑫李理张彤范思远孙阔王璐张景然王作斌长春理工大学国家纳米操纵与制造国际联合研究中心长春130012

首先从冰的2种形成机理-均相成核和非均相成核进行了阐述,并介绍了液体在固体表面上的润湿现象。在此基础上,根据微纳结构防冰机理,分别综述了在固体表面结冰的前、中、后不同时期的最新防冰除冰策略。结冰前,借助液滴的脱落和自除预防... 详细信息

首先从冰的2种形成机理-均相成核和非均相成核进行了阐述,并介绍了液体在固体表面上的润湿现象。在此基础上,根据微纳结构防冰机理,分别综述了在固体表面结冰的前、中、后不同时期的最新防冰除冰策略。结冰前,借助液滴的脱落和自除预防固体表面结冰;结冰时,可通过延长冰的冻结时间,延缓冰在固体表面上的覆盖;结冰后,降低冰在固体表面的附着力,使之更易脱离固体表面,达到防冰除冰的需求。最后,对于如何提高微纳结构表面的耐久性进行了总结,对有关这些问题的最新研究成果进行了归纳和讨论,并对今后微纳结构表面防冰除冰的研究进行了展望。

关键词：超疏水微纳结构防冰表面被动防冰方法耐久性

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Magnetic Ganoderma Lucidum Spores(mGLS):A Novel Regulatable Targeted Drug Delivery System

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Journal of Bionic Engineering 2021年第4期18卷 915-926页

作者： Bin Han Zhankun Weng Yuhan Wu Xin Zhao Jingmei Li Qinhan Zhang Kaige Qu Bojian Liang Fenguo Zhou Guixia Liu zuobin wang Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturings Changchun University of Science and Technology Changchun 130022China International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and TechnologyChangchun 130022China School of Life Sciences Changchun University of Science and TechnologyChangchun 130022China Department of Orthopaedic Surgery China-Japan Union Hospital of Jilin UniversityChangchun 130033China School of Chemistry and Environmental Engineering Changchun University of Science and TechnologyChangchun 130022China

In the past decades,many materials have been studied as carriers for targeted drug delivery.However,there is a need for utilizable and selective carrier materials with few side effects.Here,the magnetic Ganoderma Lucidum Spores(mGLS)as a highly efficient targeted drug delivery carrier were explored.Then the regulatable targeted drug delivery system was verified by loading and releasing of the 5-Fluorouracil(5-FU).The results showed that the maximum of the loaded 5-FU reached 250.23 mg·g^(−1)in the mGLS.The cumulative release of the 5-FU for the drug delivery system could reach 80.11%and 67.14%in the PBS and HCl after 48 h,respectively.In addition,this system showed the good pharmacokinetic properties in vivo.After 12 h,the blood concentration in the 5-FU@mGLS group kept at 5.3µg·mL^(−1)and was four times higher than that in the 5-FU group.In summary,the GLS as a natural microscale core-shell structures appears the striking application in carrier material for oral drug delivery.

关键词： Magnetic Ganoderma Lucidum Spores(mGLS) sustained release 5-FU drug delivery

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Analysis of the Force between DNA Molecules and Substrate in AFMbased Manipulation Process

Analysis of the Force between DNA Molecules and Substrate in...

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第36届中国控制会议

作者： Tingting Huang Zhengxun Song Ying wang Feifei wang Fenfen Guo zuobin wang International Research Centre for Nano Handling and Manufacturing of China Changchun University of Science and Technology

ISBN: (纸本)9781538629185

The force acting between molecules and substrates is crucial for the manipulation process.Atomic force microscopy（AFM） is not only a versatile tool for imaging in biological research,but also a versatile one for the manipulation of biological molecules.Because of the high mechanical resolution of AFM,it is preferred for the study of the interaction of mechanics in biological systems.This work is focused on the using of the Single-molecule force spectroscopy based on AFM（AFM-SMFS） method in the process of DNA manipulation.It explored the manipulation methods based on AFM,and then introduced one of the modification methods of the DNA molecules and the AFM cantilever.Compared with the different statements of the AFM cantilever by AFM-SMFS,analyzed the adhesion force between the molecules and the substrates.

关键词： Nano-manipulation AFM DNA manipulation AFM-SMFS

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