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Enhanced autophagy protects hepatic cells from radiation injury

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中华放射医学与防护杂志 2018年第5期38卷 335-343页

作者： liu Lang liu Dengqun Wang Yu Wang Ziwen Chen Zelin Chert Jie Han Xiao liu zujuan Zhang Aihua Shi Chunmeng Key Laboratory of Environmental Pollution Monitoring and Disease Control Ministry of Education Department of Toxicology Guizhou Medical University Guiyang 550025 China State Key Laboratory of Trauma Burns and Combined Injury Third Military Medical University Chongqing 400038 China

ObjectiveTo study the influence of radiation on autophagy and its protective effect on radiation injury of hepatic cells.MethodsAutophagy in mouse liver tissues was examined by GFP-LC3 staining and Western blot. Radiation-induced hepatic injury was evaluated by ALT and AST in mouse serum, protein expressions, and H & E and TUNEL staining of liver tissue. L02 cells were used for in vitro study. Chloroquine and rapamycin were used to manipulate the level of autophagy. ResultsTotal body irradiation (TBI) of 8 Gy caused an increase of autophagy in mouse liver tissue and AST level in serum( t＝－7.47, P<0.05) at 12 h after irradiation. Irradiation significantly increased the apoptotic level in liver tissue as well. Inhibition of autophagy by chloroquine caused a further increases of AST [IR: (345.42±35.25)U/L vs. IR＋CQ: (433.42±40.07)U/L, t＝－2.86, P<0.05] and ALT [IR: (35.67±8.08)U/L vs. IR＋CQ: (98.5±26.67)U/L, t＝－3.09, P<0.05] in the serum, and it also promoted apoptosis in live tissue. However, rapamycin as an autophagy promoter showed protective effect for radiation-induced hepatic injury [AST: IR: (345.42±35.25)U/L vs. IR＋Rap: (278.42±20.09)U/L, t＝－2.86, P<0.05]. Similar changes of autophagy and apoptosis in L02 cells were also observed in the cells treated with chloroquine and rapamycin. Inhibition of autophagy by CQ caused an increase of ROS in vitro and in vivo and further increased ALT and AST levels in serum, reduced L02 cell viability. Activation of autophagy by Rap effectively reversed those changes. ConclusionsAutophagy protects hepatic cells from radiation injury by decreasing ROS induction, which provides a potential target for the development of new clinical regimens against radiation induced liver injury.

关键词：肝细胞辐射损伤保护作用临床分析

PC4 serves as a negative regulator of skin wound healing in mice

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Burns & Trauma 2020年第1期8卷 310-318页

作者： Fengying Liao Long Chen Peng Luo Zhongyong Jiang Zelin Chen Ziwen Wang Chi Zhang Yu Wang JintaoHe Qing Wang Yawei Wang Lang liu Yu Huang Huilan Wang Qingzhi Jiang Min Luo YiboGan Yunsheng liu Yang Wang Jie Wu Wentao Xie Zhuo Cheng Yali Dai Jialun Li zujuan liu Fan Yang Chunmeng Shi Institute of Rocket Force Medicine State Key Laboratory of TraumaBurns and Combined InjuryThird Military Medical University(Army Medical University)Chongqing 400038China Institute of Clinical Medicine Southwest Medical University646000 LuzhouChina Department of Toxicology Key Laboratory of Environmental Pollution Monitoring and Disease ControlMinistry of EducationGuizhou Medical University550025 GuiyangChina

Background:Human positive cofactor 4(PC4)was initially characterized as a multifunctional transcriptional cofactor,but its role in skin wound healing is still *** purpose of this study was to explore the role of PC4 in skin wound healing through PC4 knock-in mouse ***:A PC4 knock-in mouse model(PC4^(+/+))with a dorsal full-thickness wound was used to investigate the biological functions of PC4 in skin wound *** PCR,Western blot analysis and immunohistochemistry were performed to evaluate the expression of PC4;Sirius red staining and immunofluorescence were performed to explore the change of collagen deposition and *** and apoptosis were detected using Ki67 staining and TUNEL *** dermal fibroblasts were isolated from mouse skin to perform cell scratch experiments,cck-8 assay and colony formation ***:The PC4^(+/+)mice were fertile and did not display overt abnormalities but showed an obvious delay in cutaneous healing of dorsal *** staining showed insufficient re-epithelialization,decreased angiogenesis and collagen deposition,increased apoptosis and decreased cell proliferation in PC4^(+/+)*** data also showed decreased migration rate and proliferation ability in cultured primary fibroblasts from PC4^(+/+)mice in ***:This study suggests that PC4 might serve as a negative regulator of skin wound healing in mice.

关键词： Wound healing Skin PC4 Proliferation Migration Positive cofactor 4

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妊娠期腹腔镜手术17例临床分析

中国内镜杂志 2009年第2期15卷 163-165页

作者：王英红张祖娟刘娜石河子大学医学院第一附属医院妇科新疆石河子832008 石河子大学医学院新疆石河子832008

目的探讨妊娠期腹腔镜手术的可行性、安全性以及治疗效果。方法回顾性分析2005年2月～2007年10月该院收治的妊娠15～26周、盆腔肿块直径>6cm患者17例,采取腹腔镜下手术的临床资料。结果完成卵巢囊肿剔除手术16例,其中单侧卵巢囊肿13例,... 详细信息

目的探讨妊娠期腹腔镜手术的可行性、安全性以及治疗效果。方法回顾性分析2005年2月～2007年10月该院收治的妊娠15～26周、盆腔肿块直径>6cm患者17例,采取腹腔镜下手术的临床资料。结果完成卵巢囊肿剔除手术16例,其中单侧卵巢囊肿13例,双侧3例;子宫浆膜下肌瘤剔除术1例。平均手术时间32.7min;术中平均出血36mL,术后平均住院时间5.8d;未发生手术并发症。17例均已妊娠至足月分娩,在早产率、新生儿出生体重和阿氏评分未发现异常。结论妊娠期腹腔镜手术对母体或胎儿均是安全、可行的。

关键词：妊娠腹腔镜手术卵巢肿瘤

强烈淬火对20CrMnTi钢力学性能的影响

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热加工工艺 2011年第4期40卷 176-179页

作者：傅宇东何祖娟张清华刘勇哈尔滨工程大学材料科学与化学工程学院黑龙江哈尔滨150001 哈尔滨工业大学黑龙江哈尔滨150001

对20CrMnTi钢进行强烈淬火试验,研究了强烈淬火对其力学性能的影响。结果表明,分别经CaCl2水溶液、液氮及二者分级淬火数秒均获得残余压应力,且经CaCl2水溶液×1 s+液氮×4 s分级淬火获得抗拉强度、冲击韧度、表面硬度及表面残余压应力... 详细信息

对20CrMnTi钢进行强烈淬火试验,研究了强烈淬火对其力学性能的影响。结果表明,分别经CaCl2水溶液、液氮及二者分级淬火数秒均获得残余压应力,且经CaCl2水溶液×1 s+液氮×4 s分级淬火获得抗拉强度、冲击韧度、表面硬度及表面残余压应力的最大值,此工艺为较优工艺。抗拉强度与表面残余压应力线性相关。

关键词： 20CrMnTi钢强烈淬火力学性能表面残余压应力

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锆合金吸氢及脱氢过程中的组织演变

核动力工程 2017年第2期38卷 88-92页

作者：兰光友唐彬蒲永兴何祖娟刘思维袁正川胡锐中国核动力研究设计院成都610213

采用XRD分析锆合金吸氢和脱氢过程中的相组成,并通过金相检查观察其微观组织形貌。结果表明:锆合金吸氢和脱氢过程中的相组成与过程无关;微观组织形貌仅在α+δ+γ三相共存区存在明显差异,吸氢过程中氢化锆的网状特征更加明显,但在其余... 详细信息

采用XRD分析锆合金吸氢和脱氢过程中的相组成,并通过金相检查观察其微观组织形貌。结果表明:锆合金吸氢和脱氢过程中的相组成与过程无关;微观组织形貌仅在α+δ+γ三相共存区存在明显差异,吸氢过程中氢化锆的网状特征更加明显,但在其余相区,氢化锆的组织形貌仅取决于氢含量;微观形貌的差异主要是锆合金吸氢和脱氢过程中氢在基体中扩散机制不同而引起;吸氢过程中,氢在基体中扩散受晶界扩散控制,而脱氢过程中,氢在基体中扩散受晶内扩散控制。

关键词：锆合金吸氢脱氢组织演变扩散

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Zr-4合金包壳氢化样件的制备

热加工工艺 2016年第18期45卷 87-89,92页

作者：兰光友刘思维何祖娟蒲永兴中国核动力研究设计院四川成都610213

针对Zr-4合金包壳管状试样的直径小、管壁薄、长度长的特点,进行了气相定量渗氢工艺探索。通过控制通氢量获得了不同氢含量的氢化试样,研究了氢含量对氢化锆试样组织的影响。结果表明:制定的工艺参数满足Zr-4合金包壳氢化样件的制备要求... 详细信息

针对Zr-4合金包壳管状试样的直径小、管壁薄、长度长的特点,进行了气相定量渗氢工艺探索。通过控制通氢量获得了不同氢含量的氢化试样,研究了氢含量对氢化锆试样组织的影响。结果表明:制定的工艺参数满足Zr-4合金包壳氢化样件的制备要求,样件光亮、无裂纹且氢分布均匀。采用气相渗氢方法,通过精确控制通氢量可实现气相定量渗氢。

关键词： Zr-4 合金包壳气相定量渗氢氢含量

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两种绝经后压力性尿失禁大鼠模型的比较研究

中国药物与临床 2019年第23期19卷 4054-4056页

作者：单佳汪银锋刘晓霞周勇张祖娟李文娟谢臻蔚浙江省绍兴市妇幼保健院妇科 312000 浙江大学医学院附属妇产科医院妇科

目的比较2种常用绝经后压力性尿失禁动物模型的稳定性。方法48只雌性SD大鼠,随机取12只作为空白组(不做任何处理),剩余36只雌性SD大鼠随机分成3组,A组(对照组):双侧卵巢切除组;B组(阴道气囊扩张组):阴道气囊扩张+双侧卵巢切除;C组(双侧... 详细信息

目的比较2种常用绝经后压力性尿失禁动物模型的稳定性。方法48只雌性SD大鼠,随机取12只作为空白组(不做任何处理),剩余36只雌性SD大鼠随机分成3组,A组(对照组):双侧卵巢切除组;B组(阴道气囊扩张组):阴道气囊扩张+双侧卵巢切除;C组(双侧阴部神经离断组):双侧阴部神经离断+双侧卵巢切除。分别在第8周时测定空白组和对照组的卵泡刺激素(FSH)、黄体生成素(LH)和雌二醇(E2)水平,并在建模后第1周、第4周、第8周分别检测膀胱漏点压及喷嚏试验。结果第8周时对照组的FSH和LH值均高于空白组,E2值低于空白组。阴部神经离断组和阴道气囊扩张组在第1周和第4周时的膀胱漏点压均低于对照组,但在第8周时阴道气囊扩张组与对照组的差异无统计学意义,而阴部神经离断组与对照组的差异有统计学意义。喷嚏试验结果显示,建模后第1周,阴部神经离断组和阴道气囊扩张组喷嚏试验阳性率与对照组的差异均有统计学意义;第4周时,仅阴部神经离断组有统计学意义;第8周时,2组与对照组比较差异均无统计学意义。结论双侧阴部神经离断法和阴道气囊扩张法都是绝经后压力性尿失禁动物模型的可行方案,均适用于短期实验研究;而对于长期实验研究,阴部神经离断法较阴道气囊扩张法更稳定。

关键词：尿失禁,压力性绝经后期大鼠动物,模型