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新型节能BT下载协议的设计与验证

数学的实践与认识 2013年第8期43卷 134-143页

作者：赵洪傅璐璐丽水职业技术学院机电信息分院浙江丽水323000

随着因特网普及,因上网而消耗的能源迅速上升,逐渐成为世界能源消耗的主要部分之一.这些能源绝大部分消耗在终端计算机上,包括用户的个人电脑和大型数据中心的服务器等.将重点考虑用户在P2P下载(如BT下载)中,因个人电脑必须处在长期工... 详细信息

随着因特网普及,因上网而消耗的能源迅速上升,逐渐成为世界能源消耗的主要部分之一.这些能源绝大部分消耗在终端计算机上,包括用户的个人电脑和大型数据中心的服务器等.将重点考虑用户在P2P下载(如BT下载)中,因个人电脑必须处在长期工作状态而导致的能耗.我们设计了一种新型的BT下载协议,引入一种新的BT协议的网络结构,从而在不牺牲用户BT下载的速度的情况下,允许用户的个人电脑在一定时间内待机或关机,以此大量降低BT下载协议的能耗.对该协议用实验进行了验证,实验证明新型BT下载协议最高可以减少95%的能耗,同时还能缩短文件的平均下载时间.

关键词：下载程序 BT协议 P2P

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VOF法在斜拉索风雨激振数值模拟中的应用

振动工程学报 2015年第1期28卷 130-138页

作者：王剑毕继红逯鹏关健郭红梅天津大学建筑工程学院天津300072 天津城建大学土木工程学院天津300384 滨海土木工程结构与安全教育部重点实验室天津300072

将气液两相流理论与体积分数法(VOF法)相结合,提出一种模拟发生风雨激振现象时斜拉索表面水线形成和运动的新方法;应用这一方法采用计算流体软件CFX进行数值模拟得到拉索表面水线运动规律和拉索气动力;将气动升力施加到拉索上得到拉索... 详细信息

将气液两相流理论与体积分数法(VOF法)相结合,提出一种模拟发生风雨激振现象时斜拉索表面水线形成和运动的新方法;应用这一方法采用计算流体软件CFX进行数值模拟得到拉索表面水线运动规律和拉索气动力;将气动升力施加到拉索上得到拉索的振动响应;通过与风洞试验结果及混合子结构方法的计算结果进行比对,验证了这一方法的准确性与合理性;通过分析水线运动、拉索气动力变化与拉索振动间的内在联系,揭示风雨激振现象的产生机理:上水线的环向周期性振荡导致拉索升力的周期性变化,引起拉索发生大幅度振动,上水线与拉索间的共振是产生风雨激振现象的主要原因。

关键词：风雨激振斜拉索水线水膜 VOF法

Liver-resident NK cells suppress autoimmune cholangitis and limit the proliferation of CD4^(+) T cells

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Cellular & Molecular Immunology 2020年第2期17卷 178-189页

作者： Zhi-Bin Zhao Fang-Ting lu hong-Di Ma Yin-Hu Wang Wei Yang Jie Long Qi Miao Weici Zhang Zhigang Tian William M.Ridgway Jie Cao M.Eric Gershwin Zhe-Xiong Lian Department of General Surgery Guangzhou Digestive Disease CenterGuangzhou First People’s HospitalSchool of MedicineSouth China University of TechnologyGuangzhouGuangdong 510180China Chronic Disease Laboratory Institutes for Life Sciences and School of MedicineSouth China University of TechnologyGuangzhou 510006China Institute of Immunology and School of Life Sciences University of Science and Technology of ChinaHefeiAnhui 230027China Center for Reproductive Medicine Anhui Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhuiChina Department of Gastroenterology and Hepatology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Division of Rheumatology Allergy and Clinical ImmunologyUniversity of California at Davis School of MedicineDavisCAUSA Division of Immunology Allergy and RheumatologyUniversity of CincinnatiCincinnatiOH 45267USA

Liver-resident NK cells are distinct from conventional NK cells and play an important role in the maintenance of liver *** liver-resident NK cells participate in autoimmune cholangitis remains ***,we extensively investigated the impact of NK cells in the pathogenesis of autoimmune cholangitis utilizing the well-established dnTGFβRII cholangitis model,NK cell-deficient(Nfil3−/−)mice,adoptive transfer and in vivo antibody-mediated NK cell *** data demonstrated that disease progression was associated with a significantly reduced frequency of hepatic NK *** of NK cells resulted in exacerbated autoimmune cholangitis in dnTGFβRII *** further confirmed that the DX5−CD11c^(hi) liver-resident NK cell subset colocalized with CD4^(+) T cells and inhibited CD4^(+) T cell *** expression microarray analysis demonstrated that liver-resident NK cells had a distinct gene expression pattern consisting of the increased expression of genes involved in negative regulatory functions in the context of the inflammatory microenvironment.

关键词： Liver-resident NK Cholangitis CD4^(+)T cell Suppression

ZIKA virus isolated from mosquitoes:a field and laboratory investigation in China,2016

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Science China(Life Sciences) 2017年第12期60卷 1364-1371页

作者： Shihong Fu Song Song hong Liu Yuanyuan Li Xiaolong Li Xiaoyan Gao Ziqian Xu Guoping Liu Dingming Wang Zhenzao Tian Jingzhu Zhou Ying He Wenwen Lei Huanyu Wang Bin Wang Xiaoqing lu Guodong Liang State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Hangzhou 310058China Qingdao University Qingdao 266071China Shandong Provincial Research Center for Bioinformatic Engineering and Technique School of Life SciencesShandong University of TechnologyZibo 255049China National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector BiologyMinistry of HealthWHO Collaborating Centre for Tropical DieasesShanghai 200025China Center for Disease Control and Prevention of Shenyang Command Shenyang 110034China Guizhou Province Center for Disease Control and Prevention Guiyang 550004China

A field investigation of arboviruses was conducted in Dejiang, Guizhou Province in the summer of 2016. A total of 8,795 mosquitoes, belonging to four species of three genera, and 1,300 midges were collected. The mosquito samples were identified on site according to their morphology, and the pooled samples were ground and centrifuged in the laboratory. The supernatant was incubated with mosquito tissue culture cells(C6/36) and mammalian cells(BHK-21) for virus isolation. The results indicated that 40%(3,540/8,795) were Anopheles sinensis, 30%(2,700/8,795) were Culex pipiens quinquefasciatus, and 29%(2,530/8,795)were Armigeres subbalbeatus. Furthermore, a total of eight virus isolates were obtained, and genome sequencing revealed two Zika viruses(ZIKVs) isolated from Culex pipiens quinquefasciatus and Armigeres subbalbeatus, respectively; three Japanese encephalitis viruses(JEVs) isolated from Culex pipiens quinquefasciatus; two Banna viruses(BAVs) isolated from Culex pipiens quinquefasciatus and Anopheles sinensis, respectively; and one densovirus(DNV) isolated from Culex pipiens *** ZIKVs isolated from the Culex pipiens quinquefasciatus and Armigeres subbalbeatus mosquitoes represent the first ZIKV isolates in China's Mainland. This discovery presents new challenges for the prevention and control of ZIKV in China, and prompts international cooperation on this global issue.

关键词： arbovirus surveillance mosquito-borne arbovirus Japanese encephalitis virus Zika virus

斑马鱼hoxa1a基因调控颅面骨骼发育的功能研究

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中国生物工程杂志 2021年第9期41卷 20-26页

作者：武秀知王宏杰祖尧上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 上海海洋大学科技部海洋生物科学国际联合研究中心上海201306 上海海洋大学国家水生动物病原库上海201306

hox基因编码一类高度保守的转录因子家族,人类HOXA1的突变会导致阿萨巴斯卡发育不良综合征(Athabascan brainstem dysgenesis syndrome,ABDS),使人出现因颅骨异常导致的面部畸形和面部麻痹等症状。利用模式生物斑马鱼研究其同源基因hox... 详细信息

hox基因编码一类高度保守的转录因子家族,人类HOXA1的突变会导致阿萨巴斯卡发育不良综合征(Athabascan brainstem dysgenesis syndrome,ABDS),使人出现因颅骨异常导致的面部畸形和面部麻痹等症状。利用模式生物斑马鱼研究其同源基因hoxa1a的功能机制。首先利用CRISPR/Cas9技术对斑马鱼hoxa1a进行基因编辑,获得了hoxa1a基因突变,T7E1酶切结果显示F0酶切效率平均为70%。F1进一步筛选到两种突变类型,分别是插入了8 bp和删除了7 bp的杂合突变体。杂合子自交得到hoxa1a F2纯合突变体,并且测序验证hoxa1a基因突变成功。5 dpf时,hoxa1a纯合突变体出现颅面发育异常。阿尔新蓝软骨染色和茜素红硬骨染色结果表明,hoxa1a突变体中颅骨发育异常、筛骨板断裂,鳃弓发育出现缺损。成功在斑马鱼中构建ABDS疾病模型,表明hoxa1a突变会造成斑马鱼颅面骨骼发育异常,为其功能机制研究奠定了基础,为人类ABDS疾病的致病机制研究提供了新的思路。

关键词： hoxa1a基因 ABDS 颅骨发育 CRISPR/Cas9

Survey on Natural Radiosensitizing Agents Since 2000

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Journal of Chinese Pharmaceutical Sciences 2003年第3期12卷 160-163页

作者：卢佳邵宏北京大学基础医学院放射医学基础教研室北京100083 北京大学药学院药事管理与临床药学系北京100083

Actually, radiation alone is not enough to kill tumor cells efficientlybecause of the radioresistance of tumor cells. It is well known that tumors from the samehistolog-ical origin and of the same development stage are extremely heterogeneous in theirsensitivity to radiotherapy. One of the most resistant factors to radiation is that tumor cells arecommonly hypoxic. The study on radiosensitizing agents is one of the most interesting issues intumor radiotherapeutics. These radiosensitizing agents can be classified into three main categories:natural products, synthesized chemicals and gene therapeutic agents according to their origins andtherapeutic techniques. Many radiosensitizing agents have some side-effects when they are active;so, it is important and significant to do our best to find more radiosensitizing agents with higherefficiency and lower side-effects. On the other hand, the tumor cells are easy to become resistantto older radiosensitizing agents; hence, it is urgent to develop newer radiosensitizing agents forclinics. Natural products come from plants, animals and other living beings. When serving asradiosensitizing agents in tumor radiotherapy, they are more attractive and predominant than generaldrugs. The reasons include a great resource ( especially in China) , multifunctional regulation,higher effectiveness and safer clinical effects.

关键词： radiosensitizing agent natural products radiotherapy tumor cancer carcinoma

知识转化如何影响企业标准化能力——技术能力的中介效应

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中国管理科学 2021年第12期29卷 215-226页

作者：卢宏宇余晓中国计量大学经济与管理学院浙江杭州310018

高水平的标准化能力能够提高企业产品和服务质量,引领产业升级。尽管标准具有知识和技术的双重属性,但是企业的知识转化和技术能力是否影响标准化能力的提升还有待验证。研究基于知识创造理论与吸收能力理论,构建了知识转化、技术能力... 详细信息

高水平的标准化能力能够提高企业产品和服务质量,引领产业升级。尽管标准具有知识和技术的双重属性,但是企业的知识转化和技术能力是否影响标准化能力的提升还有待验证。研究基于知识创造理论与吸收能力理论,构建了知识转化、技术能力和企业标准化能力的理论模型,通过问卷调查收集了234家企业数据,并用偏最小二乘法结构方程模型进行数据分析。实证结果显示,内部知识转化有利于企业标准应用能力和标准管理能力的提升,外部知识转化有利于企业标准应用能力和标准产出能力的提升,技术能力在内部知识转化和标准产出能力之间起完全中介效应,技术能力在内部知识转化和标准应用能力、外部知识转化与标准应用能力、标准产出能力之间起部分中介效应。

关键词：知识转化企业标准化能力技术能力

培养基灌装试验在静脉用药集中调配中心的应用及实践探索

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中国药学杂志 2024年第3期59卷 281-284页

作者：张凡陆泊宇白雪邹羽真张鑫悦赵彬梅丹中国医学科学院北京协和医学院北京协和医院北京100730 香港大学李嘉诚医学院药理及药剂学系中国香港999077

目的针对静脉用药集中调配的无菌操作,我国当前缺少行之有效的人员验证措施,亟需探索无菌操作评估方法。方法使用美国静脉质量保障(intravenous quality assurance, IVQA)培养基灌装试验套件对我院混合调配人员进行无菌操作验证,初步... 详细信息

目的针对静脉用药集中调配的无菌操作,我国当前缺少行之有效的人员验证措施,亟需探索无菌操作评估方法。方法使用美国静脉质量保障(intravenous quality assurance, IVQA)培养基灌装试验套件对我院混合调配人员进行无菌操作验证,初步探索培养基灌装试验在国内静脉用药集中调配中心应用的可行性。结果培养基灌装试验操作简便,无需苛刻培养条件与检测设备,在2种不同混合调配条件下试验结果不同,可验证人员操作。结论培养基灌装试验适用于无菌调配操作的人员质控。

关键词：培养基灌装试验无菌操作质量控制静脉用药集中调配中心

Subtyping Animal Influenza Virus with General Multiplex RT-PCR and Liquichip High Throughput (GMPLex)

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Virologica Sinica 2012年第2期27卷 120-131页

作者： Zhi-feng Qin Jie Sun Ti-kang lu Shao-ling Zeng Qun-yi Hua Qing-yan Ling Shu-kun Chen Jian-qiang Lv Cai-hong Zhang Bing Cheng Zhou-xi Ruan Ying-zuo Bi Joseph J Giambrone hong-zhuan Wu Shenzhen Entry-Exit Inspection and Quarantine Bureau Shenzhen 518010China College of Animal Science South China Agricultural UniversityCruangzhou 510642China Department of Poultry Sciences Auburn UniversityAlabama 36849USA Department of Biological Sciences Alabama State UniversityAlabama 36101USA

This study developed a multiplex RT-PCR integrated with luminex technology to rapidly subtype simultaneously multiple influenza viruses. Primers and probes were designed to amplify NS and M genes of influenza A viruses HA gene of ill, H3, H5, HT, H9 subtypes, and NA gene of the N1 and N2 subtypes. Universal super primers were introduced to establish a multiplex RT-PCR （GM RT-PCR）. It included three stages of RT-PCR amplification, and then the RT-PCR products were further tested by LiquiChip probe, combined to give an influenza virus （IV） rapid high throughput subtyping test, designated as GMPLex. The IV GMPLex rapid high throughput subtyping test presents the following features： high throughput, able to determine the subtypes of 9 target genes in H1, H3, H5, H7, H9, N1, and N2 subtypes of the influenza A virus at one time; rapid, completing the influenza subtyping within 6 hours; high specificity, ensured the specificity of the different subtypes by using two nested degenerate primers and one probe, no cross reaction occurring between the subtypes, no non-specific reactions with other pathogens and high sensitivity. When used separately to detect the product of single GM RT-PCR for single H5 or N1 gene, the GMPLex test showed a sensitivity of 10-5（= 280ELDs0） forboth tests and the luminex qualitative ratio results were 3.08 and 3.12, respectively. When used to detect the product of GM RT-PCR for H5N1 strain at the same time, both showed a sensitivity of 10-4（=2800 ELD50）. The GMPLex rapid high throughput subtyping test can satisfy the needs of influenza rapid testing.

关键词： Influenza Virus General multiplex RT-PCR Iuminex assay Subtyping HA and NA genes