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Heterologous expression of the Haynaldia villosa pattern-recognition receptor CERK1-V in wheat increases resistance to three fungal diseases

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The Crop Journal 2022年第6期10卷 1733-1745页

作者： Anqi Fan Luyang Wei Xu Zhang Jia Liua Li Sun Jin Xiao Yajia wang Haiyan wang Jian Hua Ravi P.Singh zongkuan wang Xiue wang State Key Lab of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjing 210095JiangsuChina Department of Plant Biology Cornell UniversityIthacaNY 14853USA International Maize and Wheat Improvement Center(CIMMYT) El BatanTexcoco 56237Estado de MexicoMexico

Wheat production is under continuous threat by various fungal *** of multipledisease resistance genes may lead to effective disease control via the development of cultivars with broad-spectrum *** Lysin-motif(LysM)-type pattern-recognition receptors,which elicit innate immunity by recognizing fungal pathogen associated molecular patterns such as chitin,are potential candidates for such *** this study,we cloned a LysM receptor-like kinase gene,CERK1-V,from the diploid wheat relative Haynaldia ***1-V expression was induced by chitin and Blumeria graminis ***,the causal agent of wheat powdery *** overexpression of CERK1-V in wheat inhibited the development of three fungal pathogens,thereby increased resistance to powdery mildew,yellow rust,and Fusarium head ***1-V physically interacted with the wheat Lys M protein Ta CEBi ***1-V/Ta CEBi Ps interaction promoted chitin recognition and activated chitin signal transduction in *** plants with excessively high CERK1-V expression showed high resistance but abnormal plant growth,whereas plants with moderate expression level showed adequate resistance level with no marked impairment of plant *** transgenic lines,RNA-seq showed that gene expression involved in plant innate immunity was *** of genes involved in photosynthesis,ER stress and multiple phytohormone pathways was also *** expression of CERK1-V in wheat can confer disease resistance without compromising growth or defense fitness.

关键词： Wheat Fungal disease LysM PRR Haynaldia villosa CERK1-V

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FISH-based “pan” and “core” karyotypes reveal genetic diversification of Roegneria ciliaris

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Journal of Genetics and Genomics 2023年第2期50卷 135-138页

作者： Menghao Cheng Xingyue Li Huimin Cui Haojie Sun Tianshuo Deng Xinying Song Rongrong Song Tong wang zongkuan wang Haiyan wang Xiue wang State Key Laboratory of Crop Genetics and Germplasm Enhancement/Cytogenetics Institute/Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP) Nanjing Agricultural UniversityNanjingJiangsu 210095China

The genus Roegneria was firstly established with Roegneria caucasica *** as the model *** the largest genera in the tribe Triticeae(Poaceae),Roegneria includes 130 species mainly distributed in the north temperate *** are 70 Roegneria species and 22 variants,which are widely distributed in different regions of China(Lei,2018).The diversified morphology of Roegneria species has caused controversy in their *** has been argued whether Roegneria species should be included in the genus Elymus or considered as an independent genus(Lei et al.,2018).The investigation of the cytological and genomic feature would help to clarify the taxonomic placement of Roegneria.

关键词： species Roe temperate

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A chromosome-scale genome assembly of Dasypyrum villosum provides insights into its application as a broad-spectrum disease resistance resource for wheat improvement

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Molecular Plant 2023年第2期16卷 432-451页

作者： Xu Zhang Haiyan wang Haojie Sun Yingbo Li Yilong Feng Chengzhi Jiao Mengli Li Xinying Song Tong wang zongkuan wang Chunxia Yuan Li Sun Ruju Lu Wenli Zhang Jin Xiao Xiue wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingJiangsu 210095China Biotech Research Institute Shanghai Academy of Agricultural SciencesShanghai 201106China

Dasypyrum villosum is one of the most valuable gene resources in wheat improvement,especially for disease *** mining of favorable genes from *** is frustrated by the lack of a whole genome *** this study,we generated a doubled-haploid line,91C43^(DH),using microspore culture and obtained a 4.05-GB high-quality,chromosome-scale genome assembly for *** assembly contains39727 high-confidence genes,and 85.31% of the sequences are *** reciprocal translocation events were detected,and 7VS-4VL is a unique translocation in *** prolamin seed storage protein-coding genes were found to be duplicated;in particular,the genes encoding low-molecular-weight glutenin at the Glu-V3 locus were significantly *** sequencing(RNA-seq)analysis indicated that,after Blumeria graminearum *** tritici(Bgt)inoculation,there were more upregulated genes involved in the pattern-triggered immunity and effector-triggered immunity defense pathways in *** than in Triticum *** hypersensitive sequencing(MH-seq)identified two Bgt-inducible MH sites(MHSs),one in the promoter and one in the 3'terminal region of the powdery mildew resistance(Pm)gene *** site had two subpeaks and they were termed MHS1(MHS1.1/1.2)and MHS2(MHS2.1/2.2).Bgt-inducible MHS2.2 was uniquely present in ***,and MHS1.1 was more inducible in *** than in wheat,suggesting that MHSs may be critical for regulation of NLR1-V expression and plant *** summary,this study provides a valuable genome resource for functional genomics studies and *** introgression *** identified regulatory mechanisms may also be exploited to develop new strategies for enhancing Pm resistance by optimizing gene expression in wheat.

关键词： D.villosum genome crop wild relative genome evolution disease resistance RNA-seq MNase hy-persensitivityassay

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A natural variation of an SVP MADS-box transcription factor in Triticum petropavlovskyi leads to its ectopic expression and contributes to elongated glume

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Molecular Plant 2021年第9期14卷 1408-1411页

作者： Jin Xiao Yan Chen Yifan Lu Zhipeng Liu Dongmei Si Tao Xu Li Sun zongkuan wang Chunxia Yuan Haojie Sun Xu Zhang Mingxing Wen Luyang Wei Wenli Zhang Haiyan wang Xiue wang State Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjingJiangsu 210095China

Dear Editor,Triticum petropavlovskyi,commonly known as Xinjiang rice wheat,is a unique hexaploidy wheat grown in the Xinjiang *** is marked by its elongated glume(Eg)trait,which is controlled by gene P1pet located on chromosome 7A(wang et al.,2002;Watanabe and Imamura,2002).The origin of *** has been *** is generally accepted that P1pet was introduced from tetraploid polish wheat(***)via a hybridization with hexaploid common wheat(***)(Akond et al.,2008;Chen et al.,2016).

关键词： Triticum Xinjiang wheat

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Two LysM receptors from Haynaldia villosa, CEBiP1-V and CERK1-V, play positive role in resistance to wheat powdery mildew

Two LysM receptors from Haynaldia villosa, CEBiP1-V and CERK...

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2018中国遗传学会第十次全国会员代表大会暨学术讨论会

作者： Jia Liu zongkuan wang Anqi Fan Jin Xiao Haiyan wang Xiue wang State Key Lab of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University

Plant sense potential microbial pathogen using pattern recognition receptors（PRRs） to recognize pathogen-associated molecular patterns（PAMPs）. Plant LysM receptors play major roles in the perception of chitin oligosaccharides and activate downstream defense signalings. However, the function of LysM receptors in Triticeae species remains unclear. Haynaldia villosa, a diploid relative species of wheat, is highly resistant to powdery mildew（Pm）, which is caused by Bgt（Blumeria graminis f. sp. tritici）. Here, we identified 15 LysM genes from H. villosa. They were classified into five groups by their different structural characteristics. Two LysM genes CEBiP1-V（Chitin-Elicitor Binding Protein1 in V genome） and CERK1-V（Chitin Elicitor Receptor Kinase 1 in V genome） were selected for further cloning and characterization based on their up-regulated expression in response to Bgt inoculation and chitin treatment. CEBiP1-V and CERK1-V both were localized on the plasma membrane. CEBiP1-V, but CERK1-V can bind chitin in vitro. Single-cell transient overexpression of CEBiP1-V or CERK1-V led to decreased haustorium index in Pm susceptible wheat variety Yangmai158. Stable transformation of CEBiP1-V or CERK1-V into Pm susceptible wheat variety wheat variety Fielder significantly enhanced the Pm resistance at both seedling and adult stages. The CERK1-V overexpression lines exhibited constitutive MAPK3/6 activation, and their growth is significantly retarded. Taken together, our results indicate that CEBiP1-V and CERK1-V are important PRRs that function in the recognition of chitin signals to activate MAPK cascade and positively regulate wheat Pm resistance.

关键词： Haynaldia villosa wheat powdery mildew LysM receptor CERK1-V and CEBiP1-V

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HvFP3, a HMA domain protein interacted with the E3 ubiquitin ligase HvCMPG, is involved in wheat powdery mildew defense

HvFP3, a HMA domain protein interacted with the E3 ubiquitin...

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江苏省遗传学会2013年学术研讨会

作者： LI Yingbo wang Wei FEI Fei CAO Aizhong wang Haiyan XING Liping wang zongkuan CHENG Jiangyue ZHU Yanfei XIE Qi wang Xiue National Key Laboratory of Crop Genetics and Germplasm Enhancement Cytogenetics InstituteNanjing Agricultural University Institute of Genetics and Developmental Biology Chinese Academy of Sciences

Powdery mildew is one of the most serious diseases threatened wheat production for last few decades. Haynaldia villosa L., species from wheat family, shows high powdery mildew resistance and confers the resistance gene Pm21. In our previous study, by using the Barley GeneChip microarray and cDNA library screening, a HvCMPG gene encoding E3 ligase has been cloned and was found to play an important role in the defense response to the powdery mildew disease. For further elucidating the molecular mechanism, a yeast two-hybrid library of H. villosa was constructed and used to screen the interact proteins with HvCMPG. A metal transporter gene was found to be interacted with the HvCMPG, and the full length was cloned from H. villosa and was designated as HvFP3. HvFP3 can interact with and be ubiquitinated by HvCMPG in vitro. Analysis by quantitative RT-PCR showed that HvFP3 was rapidly up-regulated in the leaf of *** after Blumeria graminis ***（Bgt） inoculation. The subcellular localization of the HvFP3 was in the nucleus and plasma membrane of the epidermal cell of onion. In the transient overexpression assay, it was found that the transient over-expression of HvFP3 led to significantly decreased in the haustoria index（HI） compared with the vector control in the epidermal cell of wheat variety Yangmai158, which is moderatly susceptible to powdery mildew disease. The HvFP3 transgenic plants over-expressed in Yangmai158 and the positive transgenic plants showed improved powdery mildew resistance than the receptor. These results indicated that the HvFP3 participated in the powdery mildew defense responses.

关键词： wheat powdery mildew yeast two hybrid metal transporter

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簇毛麦3V染色体短臂特异分子标记的开发与应用

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麦类作物学报 2023年第12期43卷 1504-1513页

作者：陈晓彤张旭慕宁陈丽娟徐涛肖进袁春霞王宗宽孙丽王秀娥王海燕南京农业大学作物遗传与种质创新利用全国重点实验室/细胞遗传所/现代作物生产省部共建协同创新中心江苏南京210095

普通小麦野生近缘物种簇毛麦(Haynaldia villosa L.2 n=14,VV)3V染色体短臂(3VS)携带抗小麦条锈病基因。开发高密度的3VS特异标记是准确鉴定和追踪3VS、推进抗病基因转移和利用的重要基础。为了开发更多的均匀分布于3VS染色体臂不同区... 详细信息

普通小麦野生近缘物种簇毛麦(Haynaldia villosa L.2 n=14,VV)3V染色体短臂(3VS)携带抗小麦条锈病基因。开发高密度的3VS特异标记是准确鉴定和追踪3VS、推进抗病基因转移和利用的重要基础。为了开发更多的均匀分布于3VS染色体臂不同区段的分子标记,本研究利用簇毛麦和小麦品种中国春参考基因组序列与基因注释信息,通过比较3VS与3AS、3BS、3DS同源基因序列内含子序列差异,选择同一内含子长度差异大于10%的区域开发了104个跨越内含子(intron targeting,IT)的分子标记,在簇毛麦、簇毛麦-硬粒小麦双二倍体、硬粒小麦中引1286、中国春、普通小麦-簇毛麦二体异附加系DA1V-DA7V、小麦-簇毛麦T3VS·3DL易位系中进行扩增,筛选出3VS特异、扩增条带清晰且稳定性好的IT分子标记43个。为了验证上述标记的有效性,选择位于3VS不同区段的12个标记对[DS3V(3D)×中国春ph1b]的F_(3)群体中的148个ph1b纯合的单株进行分析,共筛选得到6种类型涉及3VS的结构变异体。进一步利用GISH/FISH技术对上述材料进行鉴定,结果与分子标记结果相吻合,证明利用外源染色体特异分子标记可有效筛选涉及外源染色体结构变异体。

关键词：簇毛麦 IT分子标记结构变异体同源基因

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预燃室湍流射流点火甲醇发动机稀薄燃烧和排放特性试验研究

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内燃机工程 2024年第2期45卷 1-7页

作者：王志刚刘宗宽车胜楠周磊卫海桥广西玉柴机器股份有限公司玉林537005 天津大学先进内燃动力全国重点实验室天津300072

基于一台四冲程单缸发动机开展预燃室湍流射流点火(turbulent jet ignition,TJI)甲醇发动机燃烧特性、性能表现和排放特性的试验研究。结果表明,TJI燃烧模式燃烧速率较快,放热率(heat release rate,HRR)峰值明显较高,且具有更短的滞燃... 详细信息

基于一台四冲程单缸发动机开展预燃室湍流射流点火(turbulent jet ignition,TJI)甲醇发动机燃烧特性、性能表现和排放特性的试验研究。结果表明,TJI燃烧模式燃烧速率较快,放热率(heat release rate,HRR)峰值明显较高,且具有更短的滞燃期和燃烧持续期。随着过量空气系数变大,缸内压力和放热率峰值变小,TJI和火花塞点火(spark ignition,SI)燃烧模式滞燃期和燃烧持续期均变长。此外,TJI燃烧模式可有效提升甲醇发动机的稀薄燃烧稳定性,可将稀燃极限拓展至过量空气系数2.0。TJI燃烧模式下平均指示压力略低于SI模式;然而对于过量空气系数大于1.1的稀燃工况,TJI燃烧模式指示燃油消耗率更低,在过量空气系数1.3时低于570 g/(kW·h),说明其具有更好的燃油经济性。TJI燃烧模式下氮氧化物排放量明显低于SI燃烧模式,过量空气系数1.1时降低约37.2%,并且在过量空气系数大于1.3的极稀燃工况具有相对较低的甲醛CH_(2)O和碳氢化合物排放。

关键词：湍流射流点火稀薄燃烧甲醇发动机压缩比

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Mechanism of Methane Addition Affects the Ignition Process of n-heptane under Dual Fuel Engine-Like Conditions

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Journal of Thermal Science 2020年第6期29卷 1638-1654页

作者： LIU zongkuan ZHOU Lei ZHAO Wanhui QI Jiayue WEI Haiqiao State Key Laboratory of Engines Tianjin UniversityTianjin 300072China

For saving energy and protecting the environment,natural gas has been widely used in internal combustion engines,which makes the study on the ignition characteristics of natural gas/diesel mixtures *** this work,the effects of trace methane addition on the ignition delay of n-heptane/air mixtures are numerically studied using a detailed n-heptane mechanism under marine engine-like *** simulations are carried out based on the software CHEMKIN-PRO 18.0 with a closed homogeneous *** show that the prolonged ignition delay times(IDs)of n-heptane/air mixtures are observed over the whole initial temperature range after methane is added,and the increment of IDs in the negative temperature coefficient(NTC)region is significantly higher than that in high temperature *** sensitivity analysis indicates that both inhibition and promotion effects of important elementary reactions on n-heptane oxidation are weakened because of methane ***,the weakening influence on the promoting effect is more *** addition,the inhibition effect of some elementary reactions that are related to the methane oxidation is ***,the IDs of n-heptane/air mixture are *** analyses of reaction rate of production(ROP)show that the both the production and consumption rates of key radicals decrease significantly in NTC region after methane is added,but it is negligible in the high temperature *** study can extend the theoretical basis of ignition characteristics of methane/n-heptane blends under elevated temperatures and pressures.

关键词： ignition methane addition n-heptane low-temperature reaction pathways

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主成分分析和灰色模型组合的身管多点烧蚀磨损量预测

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火力与指挥控制 2024年第4期49卷 142-149页

作者：康总宽闫彬周子璇宋洪震陈学军西北机电工程研究所陕西咸阳712099 华中科技大学机械科学与工程学院武汉430074

身管是火炮类武器的关键零件,对其烧蚀磨损量进行预测,有助于保持火炮作战效能。针对火炮身管沿轴向各点烧蚀磨损量需分别建立数学模型进行预测问题,提出一种组合烧蚀磨损量预测方法。采用主成分分析(principal component analysis,PCA... 详细信息

身管是火炮类武器的关键零件,对其烧蚀磨损量进行预测,有助于保持火炮作战效能。针对火炮身管沿轴向各点烧蚀磨损量需分别建立数学模型进行预测问题,提出一种组合烧蚀磨损量预测方法。采用主成分分析(principal component analysis,PCA)方法对身管多点烧蚀磨损量进行数据空间降维,提取反映烧蚀磨损量变化的主成分,利用灰色模型对主成分进行多步预测,通过PCA逆运算获得身管内膛多点烧蚀磨损量预测值。结果表明,在历史数据较少的条件下,通过选择合适的预测步数可获得较为准确的预测值,为身管内膛多点烧蚀磨损量的预测提供了一种新的有效途径。

关键词：身管烧蚀磨损主成分分析灰色模型

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