Heterologous expression of the Haynaldia villosa pattern-recognition receptor CERK1-V in wheat increases resistance to three fungal diseases

作     者：Anqi Fan Luyang Wei Xu Zhang Jia Liua Li Sun Jin Xiao Yajia Wang Haiyan Wang Jian Hua Ravi P.Singh Zongkuan Wang Xiue Wang Anqi Fan;Luyang Wei;Xu Zhang;Jia Liu;Li Sun;Jin Xiao;Yajia Wang;Haiyan Wang;Jian Hua;Ravi P.Singh;Zongkuan Wang;Xiue Wang

作者机构：State Key Lab of Crop Genetics and Germplasm EnhancementCytogenetics InstituteNanjing Agricultural University/JCIC-MCPNanjing 210095JiangsuChina Department of Plant BiologyCornell UniversityIthacaNY 14853USA International Maize and Wheat Improvement Center(CIMMYT)El BatanTexcoco 56237Estado de MexicoMexico 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第6期

页      面：1733-1745页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Natural Science Foundation of China(31661143005,31801350 and 32011530167) National Key Research and Development Program of China(2016YFD0102001-004) China Postdoctoral Science Foundation(2018M642266) 

主　　题：Wheat Fungal disease LysM PRR Haynaldia villosa CERK1-V 

摘      要：Wheat production is under continuous threat by various fungal *** of multipledisease resistance genes may lead to effective disease control via the development of cultivars with broad-spectrum *** Lysin-motif(LysM)-type pattern-recognition receptors,which elicit innate immunity by recognizing fungal pathogen associated molecular patterns such as chitin,are potential candidates for such *** this study,we cloned a LysM receptor-like kinase gene,CERK1-V,from the diploid wheat relative Haynaldia ***1-V expression was induced by chitin and Blumeria graminis ***,the causal agent of wheat powdery *** overexpression of CERK1-V in wheat inhibited the development of three fungal pathogens,thereby increased resistance to powdery mildew,yellow rust,and Fusarium head ***1-V physically interacted with the wheat Lys M protein Ta CEBi ***1-V/Ta CEBi Ps interaction promoted chitin recognition and activated chitin signal transduction in *** plants with excessively high CERK1-V expression showed high resistance but abnormal plant growth,whereas plants with moderate expression level showed adequate resistance level with no marked impairment of plant *** transgenic lines,RNA-seq showed that gene expression involved in plant innate immunity was *** of genes involved in photosynthesis,ER stress and multiple phytohormone pathways was also *** expression of CERK1-V in wheat can confer disease resistance without compromising growth or defense fitness.