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Journal of Oceanology and Limnology 2018年第4期36卷 1342-1348页

作者： YANG Yang LIU Qinghua MA Daoyuan song zongcheng LI Jun Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Weihai Shenghang Aquatic Product Science and Technology Co. Ltd. Weihai 264200 China

Some germ cell marker genes, such as vasa, nanos, and dead end( dnd), have been identified in fish. Recently, lymphocyte antigen 75(Ly75/CD205) has been identified as a mitotic germ cell-specific cell-surface marker in several fish species. In this study, the Japanese flounder Paralichthys olivaceus ly75 homolog( ly75) was cloned and its expression pattern in gonads was analyzed. The full-length c DNA of ly75 was 7 346 bp, with an open reading frame(ORF) of 5 229 bp. The ORF encoded a protein containing 1 742 amino acids with a predicted molecular mass of 196.89 k Da. In adult tissues, ly75 transcripts were detected in all analyzed tissues but abundantly in the testis. In in-situ hybridization analyses, ly75 mRNA was predominantly localized in oocytes in the ovary and spermatogonia in the testis, but ly75 mRNA was not detected in oogonia, spermatocytes, spermatids, or spermatozoa. These results indicated that ly75 could be a potential germ cell-specific marker in P. olivaceus, as in other fishes.

关键词： Ly75/CD205 Japanese flounder Paralichthys olivaceus gonads germ cell-specific marker

Successful transplantation of cryopreserved spermatogonia in Sebastes schlegelii:A simple and suitable alternative approach for conservation of viviparous fish

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Water Biology and Security 2023年第3期2卷 75-86页

作者： Li Zhou Xueying Wang Shuran Du Shihong Xu Yanfeng Wang zongcheng song Qinghua Liu Jun Li The Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao266071China College of Life Science Ningde Normal UniversityEngineering Research Center of Mindong Aquatic Product Deep-ProcessingFujian Province UniversityNingde352100China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao266071China Weihai Shenghang Aquatic Product Science and Technology Co.Ltd. Weihai264200China

Black rockfish(Sebastes schlegelii)is one of the most important marine economic viviparous ***,germplasm degradation and genetic diversity reduction have occurred due to overfishing and long-term artificial *** cell transplantation combined with cryopreservation may be an alternative way to protect genetic ***,in viviparous fish that undertake fertilization and embryo development in vivo,transplantation is more difficult than in oviparous fish,including selection of transplantation stage,isolation of germ stem cells,and preparation of sterile *** seriously restricts the development of viviparous ***,in this study,we aimed to explore a transplantation method suitable for these *** cells were isolated from cryopreserved whole testes of 300–400g male Sebastes schlegelii in May,labeled by PKH26,and intra-peritoneally transplanted into allogeneic larvae at 5–10 days ***,the development of donor-derived cells in recipients were continuously detected by fluorescence labeling,histology,microsatellite markers,and fecundity *** results showed that donors were rich in spermatogonia(75%)and recipients maintained a high survival rate after transplantation,with a rate of>20%at sexual ***,donor-derived cells successfully migrated(100%),colonized,and incorporated into the developing recipient gonad(93.33%).Finally,transplanted recipients could normally develop and differentiate into male and female individuals,with donor-derived gametes found in 65.38%of mature *** the present study,we first establish a simple and suitable transplantation method for Sebastes schlegelii using immature males and specific larvae,which will serve as a promising tool in the protection of germplasm resources for this transplantation-restricted marine viviparous species.

关键词： Allogeneic transplantation Spermatogonia Sebastes schlegelii Germ cells Germplasm

Characterization and differential expression of three GnRH forms during reproductive development in cultured turbot Schophthalmus maximus

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Journal of Oceanology and Limnology 2018年第4期36卷 1360-1373页

作者： ZHAO Chunyan XU Shihong FENG Chengcheng LIU Yifan YANG Yang WANG Yanfeng XIAO Yongshuang song zongcheng LIU Qinghua LI Jun Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China University of Chinese Academy of Sciences Beijing 100049 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266237 China Weihai Shenghang Aquatic Product Science and Technology Co. Ltd. Weihai 264200 China

Turbots( Schophthalmus maximus), one of the most important economic marine flatfish species, fail to undergo final spawning and spermiation naturally under artificial farming conditions. In vertebrates, reproduction is regulated by the brain-pituitary-gonadal axis(BPG-axis), and gonadotropin releasing hormone(GnRH) is one of its key components. Therefore, to better understand the physiology of reproduction in the turbot, three of the genes encoding GnRH subtypes— sbGnRH, c GnRH-II and sGnRH —were cloned and sequenced by isolating the cDNA sequences. The localizations and patterns of expression of their mRNAs were also evaluated during seasonal gonadal development. All three mRNAs were expressed abundantly in the brain; sbGnRH and sGnRH mRNAs were also detected in the gonads and pituitary gland, and sbGnRH expression was much higher than that of sGnRH, indicating the critical role of sbGnRH in regulating the BPG-axis. Moreover, the brain expression patterns of sbGnRH and sGnRH mRNAs showed an increased trend during gonadal development, peaking in mature stages. This indicated the direct regulation of gonadal development by the GnRH system. In addition, c GnRH-II mRNA expression showed no significant variations, suggesting that c GnRH-II is not critically involved in the control of reproduction. Further, the mRNA abundances of the three GnRH forms in the breeding season were significantly higher than those in immature and post-breeding stages in all analyzed brain areas. Therefore, we propose that sbGnRH is the most important hormone for the regulation of reproduction in turbot via the BPG-axis. These results will help in better understanding the reproductive endocrine mechanisms of turbots and lay the groundwork for additional studies aimed at comparing the reproductive physiology of wild individuals with those raised under artificial conditions.

关键词： turbot GnRH brain gonad reproductive development

温度和盐度的变化对美国红鱼受精卵孵化的影响

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海洋湖沼通报 2000年第1期 30-35页

作者：原永党宋宗诚阮树会曲永琪威海市环翠区水产研究所威海264200 威海羊亭水产总公司威海264200

报道了美国红鱼受精卵孵化过程中，温度和盐度这二个变量对孵化率及孵化速度的影响，试验结果证明，水温２５—２８℃、盐度２８—３０‰为最适范围，其孵化率最高，孵化速度最快。

关键词：美国红鱼孵化率温度盐度受精孵化红鱼

星康吉鳗发育过程中个体形态学和卵母细胞特征变化及组织营养成分分析

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上海海洋大学学报 2021年第3期30卷 442-453页

作者：吴盟鲁强刘如聪江兴龙宋宗诚李慷刘利平上海海洋大学水产种质资源发掘与利用教育部重点实验室上海201306 鳗鲡现代产业技术教育部工程研究中心福建厦门361021 威海圣航水产科技有限公司山东威海264299 上海海洋大学上海水产养殖工程技术研究中心上海201306

对星康吉鳗(Conger myriaster)卵巢发育过程中的个体形态学和卵母细胞特征变化,以及卵巢、肝脏、肌肉中营养成分的变化情况进行研究。结果表明:星康吉鳗卵巢发育分为5个时期,即卵母细胞单层滤泡期、卵母细胞出现脂肪泡期、卵母细胞卵黄... 详细信息

对星康吉鳗(Conger myriaster)卵巢发育过程中的个体形态学和卵母细胞特征变化,以及卵巢、肝脏、肌肉中营养成分的变化情况进行研究。结果表明:星康吉鳗卵巢发育分为5个时期,即卵母细胞单层滤泡期、卵母细胞出现脂肪泡期、卵母细胞卵黄充满期(早期、中期、末期)、卵母细胞核极化期和卵母细胞成熟期。卵母细胞直径、细胞核直径、脂肪泡直径和卵黄球直径随着卵母细胞的发育逐渐增长。卵母细胞中脂肪泡的初始发生位点在细胞核周围,并且发生时间上脂肪泡先于卵黄球,随着卵母细胞发育,脂肪泡呈环状分布并逐渐向细胞膜方向延伸;卵母细胞中卵黄球的初始发生位点在细胞膜周边的细胞质层,随着卵母细胞发育,卵黄球呈环状分布并向细胞核方向延伸。发育过程中星康吉鳗性体指数、肝体指数、眼径指数、肛长指数之间均呈正相关性;消化道指数与性体指数、肝体指数、眼径指数、肛长指数分别呈负相关性。营养成分变化表明,星康吉鳗主要储存脂肪的位置在肌肉,脂肪的转运在星康吉鳗的卵巢发育中起到了重要作用。研究结果可为星康吉鳗全人工繁殖提供参考。

关键词：星康吉鳗性腺发育卵母细胞个体形态学营养成分

人工诱导星康吉鳗卵巢发育主要组织脂质代谢分析

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水产学报 2024年第8期48卷 91-101页

作者：王桂香李慷刘如聪姜之信巴旭冰宋宗诚刘利平上海海洋大学水产种质资源发掘与利用教育部重点实验室上海海洋大学上海水产养殖工程技术研究中心上海海洋大学水产科学国家级实验教学示范中心威海圣航水产科技有限公司

为分析人工诱导星康吉鳗卵巢发育组织脂质代谢，实验使用超高效液相色谱四极杆轨道阱质谱(UHPLC/Q-Orbitrap MS)对人工诱导星康吉鳗卵巢发育不同时期肝脏、卵巢、肌肉和血浆进行了脂质组学分析，并测定了肝脏代谢酶活。对不同发育阶段... 详细信息

为分析人工诱导星康吉鳗卵巢发育组织脂质代谢，实验使用超高效液相色谱四极杆轨道阱质谱(UHPLC/Q-Orbitrap MS)对人工诱导星康吉鳗卵巢发育不同时期肝脏、卵巢、肌肉和血浆进行了脂质组学分析，并测定了肝脏代谢酶活。对不同发育阶段星康吉鳗组织脂质成分分析结果显示，在肝脏、肌肉、卵巢和血浆中各鉴定出68、27、63和212种脂质标志物，主要包括磷脂酰丝氨酸(PS)、磷脂酰乙醇胺(PE)、磷脂酰胆碱(PC)、甘油三酯(TG)和鞘磷脂(SM)等。4种组织中TG、甘油二酯(DG)和神经酰胺(Cer)等脂类物质在发育过程中呈现出不同的变化趋势。肝脏代谢中，脂肪酸合成酶、肝脂酶和脂蛋白脂酶浓度呈现先升高后降低的过程，主要参与了TG、磷脂和脂肪酸的代谢。研究表明，星康吉鳗卵巢发育过程中主要组织的TG、磷脂发生了不同程度的变化，脂质代谢相关酶活性变化也与脂质代谢特征相对应。

关键词：星康吉鳗脂质转化脂质组学脂质代谢

三倍体牙鲆(Paralichthys olivaceus)群体遗传多样性的微卫星分析

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渔业科学进展 2015年第1期36卷 33-40页

作者：刘慧吴志昊李桢朱香萍尤锋宋宗诚李军胡金伟青岛农业大学青岛266109 中国科学院实验海洋生物学重点实验室中国科学院海洋研究所青岛266071 威海圣航水产科技有限公司威海264200 海洋生物工程技术研究发展中心中国科学院海洋研究所青岛266071

采用微卫星遗传标记技术对三倍体牙鲆群体及二倍体对照群体进行比较分析。取2012年冷休克诱导和培育至11月龄的三倍体及同期二倍体对照牙鲆个体各32尾,通过高盐法提取肌肉组织总DNA;利用筛选得到的21对微卫星引物进行PCR扩增,并经14%聚... 详细信息

采用微卫星遗传标记技术对三倍体牙鲆群体及二倍体对照群体进行比较分析。取2012年冷休克诱导和培育至11月龄的三倍体及同期二倍体对照牙鲆个体各32尾,通过高盐法提取肌肉组织总DNA;利用筛选得到的21对微卫星引物进行PCR扩增,并经14%聚丙烯酰胺凝胶电泳分型后,进行人工判读和分析。结果显示,21个微卫星位点均为多态,二倍体和三倍体群体的平均等位基因数(A)、基因型总数、平均有效等位基因数(ae)、平均观测杂合度(HO)和平均期望杂合度(HE)分别为5.6和5.0、190和142、3.7和2.8、0.613和0.868、0.681和0.629。二倍体和三倍体群体的多态信息含量(PIC)分别为0.642和0.589,个体识别率(DP)为0.660和0.609,非父排除率(PPE)为0.482和0.399,其累积个体识别率和非父排除率均达到0.999,表明所选座位均属中高识别力的遗传标记,可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。二倍体和三倍体群体间的遗传距离(D)为0.312,遗传相似系数(I)为0.732,基因分化系数(GST)为0.971。以上结果说明,三倍体诱导对牙鲆的遗传结构造成了一定影响,导致三倍体遗传多样性水平相对二倍体有所下降,二倍体和三倍体牙鲆的基因型也存在一定差异。

关键词：牙鲆三倍体人工诱导微卫星遗传多样性

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大叶藻形态特征与显微结构

海洋湖沼通报 2010年第3期 73-78页

作者：原永党宋宗诚郭长禄刘振林谷杰泉刘海燕施坤涛威海市环翠区海洋与渔业研究所山东威海264200 哈尔滨工业大学(威海)海洋学院山东威海264209

大叶藻(Zostera marina)是陆生植物起源,在海洋中进行沉水生活的高等单子叶植物。本文主要研究大叶藻的形态特征和组织结构与适水性生活的相关性,通过制作、观察大叶藻石蜡切片,介绍大叶藻的根、茎、叶及种子的显微结构特点。结果发现... 详细信息

大叶藻(Zostera marina)是陆生植物起源,在海洋中进行沉水生活的高等单子叶植物。本文主要研究大叶藻的形态特征和组织结构与适水性生活的相关性,通过制作、观察大叶藻石蜡切片,介绍大叶藻的根、茎、叶及种子的显微结构特点。结果发现大叶藻的形态特征和显微结构与沉水生活具有高度的适应性。

关键词：大叶藻形态结构石蜡切片

Effects of synthesis methods on the performance of Pt + Rh/Ce_(0.6)Zr_(0.4)O_2 three-way catalysts

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Journal of Environmental Sciences 2014年第3期26卷 683-693页

作者： zongcheng Zhan Liyun song Xiaojun Liu Jiao Jiao Jinzhou Li Hong He Laboratory of Catalysis Chemistry and Nano-Science Department of Chemistry and Chemical Engineering College of Environmental and Energy Engineering Beijing University of Technology

The 0.7 wt% Pt ＋ 0.3 wt% Rh/Ce0.6Zr0.4O2 catalysts were fabricated via different methods, including ultrasonic-assisted membrane reduction （UAMR） co-precipitation, UAMR separation precipitation, co-impregnation, and sequential impregnation. The catalysts were physico-chemically characterized by N2 adsorption, XRD, TEM, and Hz-TPR techniques, and evaluated for three-way catalytic activities with simulated automobile exhaust. UAMR co-precipitation- and UAMR separation precipitation- prepared catalysts exhibited a high surface area and metal dispersion, wide λ window and excellent conversion for NOx reduction under lean conditions. Both fresh and aged catalysts from UAMR- precipitation showed the high surface areas of ca. 60-67 m^2/g and 18-22 m^2/g, respectively, high metal dispersion of 41%-55%, and small active particle diameters of 2.1-2.7 nm. When these catalysts were aged, the catalysts prepared by the UAMR method exhibited a wider working window （△λ = 0.284--0.287） than impregnated ones （△λ = 0.065-0.115） as well as excellent three-way catalytic performance, and showed lower/so （169℃） and T90 （195℃） for NO reduction than the aged catalysts from impregnation processes, which were at 265 and 309℃, respectively. This implied that the UAMR-separation precipitation has important potential for industrial applications to improve catalytic performance and thermal stability. The fresh and aged 0.7 wt% Pt ＋ 0.3 wt% Rh/Ce0.6Zr0.4O2 catalysts prepared by the UAMR-separation precipitation method exhibited better catalytic performance than the corresponding catalysts prepared by conventional impregnation routes.

关键词： UAMRthree way catalyst Pt and Rh nanoparticles precipitation thermal stability

不同生长时期大菱鲆(Scophthalmus maximus)形态性状与体质量的通径分析及曲线拟合研究

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广西科学院学报 2018年第3期34卷 181-190页

作者：刘莹于超勇于道德宋静静宋宗诚赵文溪刘洪军官曙光山东省海洋生物研究院山东省海水健康养殖工程技术研究中心山东省海水养殖病害防治重点实验室山东青岛266104 威海圣航水产科技有限公司山东威海264200

【目的】深入了解不同生长时期大菱鲆(Scophthalmus maximus)形态性状与体质量之间的关系,确定适于大菱鲆人工选育的主要测量指标。【方法】应用相关性分析、通径分析和多元回归分析方法对大菱鲆6月龄幼鱼及14月龄成鱼全长(TL)、体长(BL... 详细信息

【目的】深入了解不同生长时期大菱鲆(Scophthalmus maximus)形态性状与体质量之间的关系,确定适于大菱鲆人工选育的主要测量指标。【方法】应用相关性分析、通径分析和多元回归分析方法对大菱鲆6月龄幼鱼及14月龄成鱼全长(TL)、体长(BL)、头长(HL)、体高(BD)、尾柄宽(THH)、体厚(BW)和体质量(BM)7个性状进行分析,同时通过曲线拟合分析获得形态性状与体质量之间的最佳拟合模型。【结果】不同生长时期,大菱鲆各形态性状与体质量的相关性均达到极显著水平(P<0.01)。6月龄阶段,全长(TL)、体高(BD)、体厚(BW)、头长(HL)的直接通径系数达到显著水平(P<0.05);14月龄阶段,全长(TL)、体厚(BW)、尾柄宽(THH)的直接通径系数达极显著水平(P<0.01),并建立2个生长时期不同性状对体质量的回归方程。6月龄阶段,进入回归方程的各形态性状与体质量(BM)的最优曲线拟合模型均为线性函数模型;14月龄阶段,进入回归方程的各形态性状与体质量(BM)的最优曲线拟合模型均为幂函数模型。【结论】不同时期,影响大菱鲆体质量的主要形态性状不同,且各形态性状对大菱鲆体质量的作用效果也不尽相同,适用的最优拟合模型也不同。建议将全长(TL)(6~14月龄)作为幼鱼与成鱼阶段的主要育种目标性状,同时幼鱼阶段辅以体高(BD)、体厚(BW)和头长(HL)作为参考性状,成鱼阶段辅以体厚(BW)和尾柄宽(THH)为参考性状,从而有效提高大菱鲆的选育效率,为大菱鲆选育提供测量指标与理论支持。

关键词：大菱鲆形态性状体质量相关分析通径分析模型拟合