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基于荧光方法的循环肿瘤细胞检测研究进展

发光学报 2024年第6期45卷 1015-1032页

作者：杨瑞李子越申艺郭丽华周兵帅李家玮刘海鹏董彪吉林大学电子科学与工程学院集成光电子学国家重点实验室吉林长春130012 吉林大学第一医院整形美容与修复重建外科吉林长春130012

循环肿瘤细胞(Circulating tumor cells,CTCs)是指从恶性肿瘤的原发或转移部位脱落的细胞,通过血液循环到达全身。体内CTCs的存在可以反映肿瘤的发生与发展,对肿瘤的诊断和预后至关重要。然而,实现高纯度捕获和捕获后CTCs灭活阻断仍然... 详细信息

循环肿瘤细胞(Circulating tumor cells,CTCs)是指从恶性肿瘤的原发或转移部位脱落的细胞,通过血液循环到达全身。体内CTCs的存在可以反映肿瘤的发生与发展,对肿瘤的诊断和预后至关重要。然而,实现高纯度捕获和捕获后CTCs灭活阻断仍然面临许多挑战。目前开发的用于实现选择性分离CTCs的方案中,荧光方法由于具有高灵敏度、高分辨率、操作简便等特点,在无创检测和快速检测方面具有重要的应用前景。与以往的CTCs研究综述相比,本文详细介绍了CTCs从体外捕获到体内捕获再到下游分析的全过程,并对CTCs的完整诊疗过程进行了系统和详细的总结,为当前的研究提供了新思路,这对于实现早期循环肿瘤细胞的诊断与治疗具有较重要意义。

关键词：循环肿瘤细胞荧光捕获下游分析灭活

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复合绝缘子酥朽断裂事故的分布规律研究

高压电器 2024年第2期60卷 133-142页

作者：卢明高超李子岳吴焱松梅红伟王黎明河南省电力线路舞动防治技术重点实验室国网河南省电力公司电力科学研究院郑州450052 清华大学深圳国际研究生院广东深圳518055

论文在调研了2010—2020年国网范围内发生的复合绝缘子酥朽断裂事故的基础上,总结了复合绝缘子酥朽断裂事故在中国的地域分布特征,研究了自然环境因素以及运行条件与酥朽断裂事故的关联关系。复合绝缘子酥朽断裂事故集中分布在中国东部... 详细信息

论文在调研了2010—2020年国网范围内发生的复合绝缘子酥朽断裂事故的基础上,总结了复合绝缘子酥朽断裂事故在中国的地域分布特征,研究了自然环境因素以及运行条件与酥朽断裂事故的关联关系。复合绝缘子酥朽断裂事故集中分布在中国东部沿海及南方地区。根据统计,90%左右的事故绝缘子都位于年降水量在500 mm以上的地区,事故发生前处于相对湿度60%以上的环境中。酥断事故在任何环境温度下均有发生的可能,但仍集中分布在20℃以上的环境温度下。除了环境温度、湿度,酥朽断裂事故的分布与地形、海拔、风力等其他自然因素并无明显的相关性。与酥朽断裂相关性较大的运行条件是电压等级和串型。通过电场计算,高压侧局部场强过大是造成酥朽断裂事故集中分布在500 kV电压等级线路绝缘子的重要原因,但不是V串500 kV复合绝缘子酥朽断裂事故多发的原因。

关键词：复合绝缘子酥朽断裂自然环境运行状况电场计算

Task-Oriented Topology System Synthesis of Reconfigurable Legged Mobile Lander Integrating Active and Passive Metamorphoses

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Chinese Journal of Mechanical Engineering 2023年第3期36卷 89-109页

作者： Youcheng Han ziyue li Gaohan Zhu Weizhong Guo Jianzhong Yang Wei liu State Key Lab of Mechanical System and Vibration School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Beijing Institute of Spacecraft System Engineering Beijing 100094China

To explore hostile extraterrestrial landforms and construct an engineering prototype,this paper presents the task-oriented topology system synthesis of reconfigurable legged mobile lander(ReLML)with three operation modes from adjusting,landing,to *** with our preceding works,the adjusting mode with three rotations(3R)provides a totally novel exploration approach to geometrically matching and securely arriving at complex terrains dangerous to visit currently;the landing mode is redefined by two rotations one translation(2R1T),identical with the tried-and-tested Apollo and Chang'E landers to enhance survivability via reasonable touchdown buffering motion;roving mode also utilizes 2R1T motion for good motion and force *** reconfigurable mechanism theory is first brought into synthesizing legged mobile lander integrating active and passive metamorphoses,composed of two types of metamorphic joints and metamorphic execution and transmission *** reveal metamorphic principles with multiple finite motions,the finite screw theory is developed to present the procedure from unified mathematical representation,modes and source phase derivations,metamorphic joint and limb design,to final structure *** identify the prototype topology,the 3D optimal selection matrix method is proposed considering three operation modes,five evaluation criteria,and two topological ***,simulation verifies the whole task implementation process to ensure the reasonability of design.

关键词： Legged mobile lander Topology synthesis Active and passive metamorphoses Finite screw Metamorphic joint Reconfigurable mechanism

1990-2019年中国痛风疾病负担趋势及未来十年预测研究

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中国全科医学 2024年第2期27卷 245-252页

作者：方珈文冯天元周志衡李子悦颜丹虹林凯程南方医科大学卫生管理学院广东省广州市510515 广东省深圳市坪山区人民医院 518118 南方医科大学南方医院广东省广州市510515

背景痛风是常见的炎症性关节炎之一,对人们的健康造成很大的危害,但目前针对我国痛风疾病负担趋势分析与预测的相关研究有限。目的分析1990—2019年中国痛风疾病负担的变化趋势,并预测未来10年痛风的标化伤残调整寿命年(DALYs)率、标化... 详细信息

背景痛风是常见的炎症性关节炎之一,对人们的健康造成很大的危害,但目前针对我国痛风疾病负担趋势分析与预测的相关研究有限。目的分析1990—2019年中国痛风疾病负担的变化趋势,并预测未来10年痛风的标化伤残调整寿命年(DALYs)率、标化发病率和标化患病率。方法提取全球疾病负担研究(GBD)2019有关中国痛风DALYs、发病情况和患病情况等疾病负担指标,采用Joinpoint回归模型计算标化DALYs率、标化发病率和标化患病率的年度变化百分比(APC)和平均年度变化百分比(AAPC),采用自回归移动平均模型(ARIMA模型)对2020—2029年的标化DALYs率、标化发病率和标化患病率进行预测。结果1990—2019年,中国痛风DALYs从187436人年增至510485人年,增长了172.35%,发病人数从1181969例增至3041329例,增长了157.31%,患病人数从5864143例增至16161325例,增长了175.60%(对应的标化率分别增长了28.45%、25.92%和28.63%)。Joinpoint回归结果显示,1990—2019年,标化DALYs率、标化发病率、标化患病率整体呈上升趋势(AAPC分别为0.9%、0.8%、0.9%,P<0.05)。痛风疾病负担具有明显的性别和年龄差异,男性疾病负担高于女性,标化DALYs率、标化发病率和标化患病率男女性别比范围分别为3.23~3.51、3.14~3.40和3.17~3.42。女性各指标年龄高峰总体比男性延后,女性在45岁之后疾病负担开始加重,男女性疾病负担均在90岁之后再次增加。ARIMA模型结果显示,预计到2029年,因痛风造成的标化DALYs率和标化发病率分别下降0.33%和0.45%,标化患病率上升0.71%。结论1990—2019年中国痛风疾病负担增长趋势明显,存在明显的性别和年龄差异,且出现年轻化趋势。预计到2029年,因痛风造成的标化患病率略有上升。

关键词：痛风发病率患病率疾病负担趋势分析 ARIMA模型伤残调整寿命年

基于BSA-seq技术对乌拉尔图小麦条锈病抗病基因的初步定位

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河南农业大学学报 2024年第1期58卷 44-51页

作者：倪楠楠杨小妮赵建辉李子玥乔柳惠李广伟王道文王换河南农业大学农学院/省部共建小麦玉米作物学国家重点实验室河南郑州450046

【目的】发掘和克隆小麦条锈病抗病基因,为小麦条锈病抗病育种提供遗传基因资源。【方法】以乌拉尔图小麦为材料,通过接种鉴定筛选具有条锈病抗性的材料,利用正向遗传学和混合分组测序分析法(BSA-seq)在乌拉尔图小麦材料中鉴定新的条锈... 详细信息

【目的】发掘和克隆小麦条锈病抗病基因,为小麦条锈病抗病育种提供遗传基因资源。【方法】以乌拉尔图小麦为材料,通过接种鉴定筛选具有条锈病抗性的材料,利用正向遗传学和混合分组测序分析法(BSA-seq)在乌拉尔图小麦材料中鉴定新的条锈病抗病基因位点。【结果】通过接种鉴定筛选到6个具有小麦条锈病抗性的乌拉尔图小麦材料,通过构建杂交群体结合BSA-seq技术在其中1个抗病材料TuW1中鉴定出1个新的条锈病抗病基因位点,定位于7AS染色体的0~35.2 Mb区间。【结论】通过正向遗传学结合BSA-seq技术在乌拉尔图小麦中鉴定到1个新的条锈病抗病基因位点,为小麦条锈病抗病育种提供了基因资源。

关键词：乌拉尔图小麦条锈病抗病基因混合分组测序分析法遗传定位

Launching, perceiving, and diminishing of airborne signals

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Molecular Plant 2023年第12期16卷 1882-1884页

作者： Mikayla Carty Ruize Zhang ziyue li Daowen Wang Zheng Qing Fu Department of Biological Sciences University of South CarolinaColumbiaSC29208USA State Key Laboratory of Wheat and Maize Crop Science College of Agronomyand Center for Crop Genome EngineeringHenan Agricultural UniversityZhengzhou450002China

Aphids are highly destructive pests that greatly hurt the agricultural *** over 40%of plant viruses,they are the most destructive pests that the agricultural industry faces(Gong et al.,2023).Aphids feed on plants,and in doing so,they consume sap from one plant and then move to another and inject their saliva into the new plant(Hooks and Fereres,2006).This cycle causes diseases and viral pathogens to become rampant between plants as the aphids carry viral pathogens from one plant to another and so on.

关键词： plant carry

siRNA-functionalized lanthanide nanoparticle enables efficient endosomal escape and cancer treatment

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Nano Research 2022年第10期15卷 9160-9168页

作者： Chanchan Yu Kun li lin Xu Bo li Chunhui li Shuai Guo ziyue li Yuquan Zhang Abid Hussain Hong Tan Mengyu Zhang Yongxiang Zhao Yuanyu Huang Xing-Jie liang School of life Science Advanced Research Institute of Multidisciplinary ScienceSchool of Medical Technology(Institute of Engineering Medicine)Key Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety CAS Center for Excellence in NanoscienceNational Center for Nanoscience and Technology of ChinaBeijing 100190China National Center for International Research of Biological Targeting Diagnosis and Therapy Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy ResearchGuangxi Medical UniversityNanning 530021China

Attaching DNA/RNA to nanomaterials is the basis for nucleic acid-based assembly and drug ***,we report that small interfering RNA(siRNA)effectively coordinates with ligand-free lanthanide nanoparticles(NaGdF4 NPs),and forms siRNA/NaGdF4 spherical nucleic acids(SNA).The coordination is primarily attributed to the interaction between Gd and phosphate backbone of the ***,an efficient encapsulation and rapid endosomal escape of siRNA from the endosome/lysosome were achieved,due to its flexible ability to bound to phospholipid head of endosomal membrane,thereby disrupting the membrane *** to the dual properties of NaGdF4 NPs,siRNA loading,and endosomal escape,siRNA targeting programmed cell death-ligand 1(siPD-L1)/NaGdF4 SNA triggers significant gene silencing in vitro and in vivo,and effectively represses the tumor growth in both CT26 tumor model and 4T1 orthotopic murine model.

关键词： coordination chemistry nuclei acid lanthanide nanomaterials small interfering RNA(siRNA)delivery endosome escape

Elaidic acid leads to mitochondrial dysfunction via mitochondria-associated membranes triggers disruption of mitochondrial calcium fluxes

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Food Science and Human Wellness 2024年第1期13卷 287-298页

作者： Hui liu Xuenan li ziyue Wang Lu li Yucai li Haiyang Yan Yuan Yuan College of Food Science and Engineering Jilin UniversityChangchun 130062China

Elaidic acid(EA)stimulation can lead to endoplasmic reticulum stress(ERS),accompanied by a large release of Ca^(2+),and ultimately the activation of NLRP3 inflammasome in Kupffer cells(KCs).Mitochondrial instability or dysfunction may be the key stimulating factors to activate NLRP3 inflammasome,and sustained Ca^(2+)transfer can result in mitochondrial *** focused on KCs to explore the damage to mitochondria by *** EA stimulation,cells produced an oxidative stress(OS)response with a significant increase in ROS *** experiments and the addition of inhibitors revealed that the increase in the level of intracellular Ca^(2+)led to Ca^(2+)accumulation in the mitochondrial matrix via mitochondria-associated membranes(MAMs).This was accompanied by a significant release of m ROS,loss of MMP and ATP,and a significant increase in mitochondrial permeability transition pore opening,ultimately leading to mitochondrial *** findings confirmed the mechanism that EA induced mitochondrial Ca^(2+)imbalance in KCs via MAM,ultimately leading to mitochondrial ***,EA induced OS and the decrease of MMP and ATP in rat liver,and significant lesions were found in liver *** of the inner mitochondrial cristae and mitochondrial vacuolization occurred,with a marked increase in lipid droplets.

关键词： Elaidic acid(EA) Mitochondria-associated membranes(MAMs) Calcium Endoplasmic reticulum Mitochondria dysfunction

Biosafety materials:Ushering in a new era of infectious disease diagnosis and treatment with the CRISPR/Cas system

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Biosafety and Health 2022年第2期4卷 70-78页

作者： Yuquan Zhang ziyue li Julien Milon Essola Kun Ge Xuyan Dai Huining He Haihua Xiao Yuhua Weng Yuanyu Huang School of life Science School of Medical TechnologyAdvanced Research Institute of Multidisciplinary ScienceKey Laboratory of Molecular Medicine and BiotherapyKey Laboratory of Medical Molecule Science and Pharmaceutics EngineeringBeijing Institute of TechnologyBeijing 100081China Key Laboratory of Analytical Science and Technology of Hebei Province College of Chemistry and Environmental ScienceKey Laboratory of Medicinal Chemistry and Molecular Diagnosis of the Ministry of EducationHebei UniversityBaoding 071000China Hunan Agricultural University Changsha 410128China Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics School of PharmacyTianjin Medical UniversityTianjin 300070China Beijing National Laboratory for Molecular Science State Key Laboratory of Polymer Physical and ChemistryInstitute of ChemistryChinese Academy of ScienceBeijing 100190China School of Materials and the Environment Beijing Institute of TechnologyZhuhai 519085China

Despite multiple virus outbreaks over the past decade,including the devastating coronavirus disease 2019(COVID-19)pandemic,the lack of accurate and timely diagnosis and treatment technologies has wreaked havoc on global *** clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated proteins(Cas)system has the potential to address these critical needs for tackling infectious diseases to detect viral nucleic acids and inhibit viral *** review summarizes how the CRISPR/Cas system is being utilized for the treatment and diagnosis of infectious diseases with the help of biosafety materials and highlights the design principle and in vivo and in vitro efficacy of advanced biosafety materials used to deal with virus attacks.

关键词： Biosafety materials CRISPR/Cas SARS-CoV-2 Diagnosis Infectious disease