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Genome-wide identification and spatiotemporal expression profiling of zinc finger SWIM domain-containing protein family genes

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Zoological Research 2023年第3期44卷 663-674页

作者： Imtiaz Ul Hassan Hafiz Mamoon Rehman ziran liu Liangji Zhou Muhammad Kaleem Samma Chengdong Wang Zixin Rong Xufeng Qi Dongqing Cai Hui Zhao Key Laboratory for Regenerative Medicine Ministry of EducationSchool of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Centre for Agricultural Biochemistry and Biotechnology(CABB) University of Agriculture FaisalabadFaisalabad 38000Pakistan Qingdao Municipal Center for Disease Control and Prevention QingdaoShandong 266034China Key Laboratory of Regenerative Medicine of Ministry of Education Department of Developmental&Regenerative BiologyJinan UniversityGuangzhou 511436China Department of Biology and Chemistry City University of Hong KongHong Kong SARChina Department of Gene Technology School of Engineering Sciences in ChemistryBiotechnology and Health(CBH)KTH Royal Institute of Technology10691 StockholmSweden Kunming Institute of Zoology-The Chinese University of Hong Kong(KIZ-CUHK)Joint Laboratory of Bioresources and Molecular Research of Common Diseases Hong Kong SARChina Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics The Chinese University of Hong KongHong Kong SARChina

The biological function of the novel zinc-finger SWIM domain-containing protein family(ZSWIM)during embryonic development remains ***,we conducted a genome-wide analysis to explore the evolutionary processes of the ZSWIM gene family members in mice,Xenopus tropicalis,zebrafish,and *** identified nine putative ZSWIM genes in the human and mouse genome,eight in the Xenopus genome,and five in the zebrafish *** on multiple sequence alignment,three members,ZSWIM5,ZSWIM6,and ZSWIM8,demonstrated the highest homology across all four *** available RNA sequencing(RNAseq)data,ZSWIM genes were found to be widely expressed across different tissues,with distinct tissuespecific *** identify the functions of the ZSWIM protein family during embryogenesis,we examined temporal and spatial expression patterns of zswim family genes in Xenopus *** real-time polymerase chain reaction(qRT-PCR)revealed that each member had a distinct expression ***-mount in situ hybridization showed that both zswim1 and zswim3 were maternally expressed genes;zswim5 and zswim6were expressed throughout embryogenesis and displayed dynamic expression in the brain,eyes,somite,and bronchial arch at the late tailbud stages;zswim7 was detected in the eye area;zswim8 showed a dynamic expression pattern during the tailbud stages,with expression detected in the brain,eyes,and somite;zswim9 was faintly expressed throughout embryonic *** study provides a foundation for future research to delineate the functions of ZSWIM gene members.

关键词： ZSWIM gene family Xenopus Gene expression Phylogenetic analysis Bioinformatics

Long-lasting blue persistent luminescence from β-Ga2O3 with B3+ addition

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Long-lasting blue persistent luminescence from β-Ga2O3 with...

LncPheDB:a genome-wide lncRNAs regulated phenotypes database in plants

第十九届全国高技术陶瓷学术年会

作者：钟瑞霞 Jianquan Qi ziran liu School of Resources and Materials Northeastern University at Qinhuangdao Hebei province key laboratory of dielectric and electrolyte Northeastern University at Qinhuangdao Institute of Condensed Matter Physics Hebei Normal University of Science and Technology

Blue persistent luminescent β-Ga2O3: B3+ was synthesized by high solid-state reaction. The phosphor exhibits more than 0.5 h afterglow in the wavelength range of 380-600 nm after ceasing the ultraviolet light（260 nm） irradiation. The emission and excitation spectra, persistent luminescence decay curve, and thermoluminescence curve were measured. The emission and excitation spectra indicate that the B3+ doping improves on photoluminescent properties of β-Ga2O3. The blue persistent luminescence of β-Ga2O3: 80% B3+ could be observed for 5 min by naked eye. The thermoluminescence（TL） curve shows that B3+ increased the traps and improved the depth of the traps.

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aBIOTECH 2022年第3期3卷 169-177页

作者： Danjing Lou Fei Li Jinyue Ge Weiya Fan ziran liu Yanyan Wang Jingfen Huang Meng Xing Wenlong Guo Shizhuang Wang Weihua Qiao Zhenyun Han Qian Qian Qingwen Yang Xiaoming Zheng National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing100081China College of Life Science Shenyang Normal UniversityShenyang110034China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanya572000China State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou310006China Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen518120China International Rice Research Institute DAPO box 7777Metro ManilaThe Philippines

LncPheDB(https://***/)is a systematic resource of genome-wide long non-coding RNAs(lncRNAs)-phenotypes associations for multiple *** was established to display the genome-wide lncRNA annotations,target genes prediction,variant-trait associations,gene-phenotype correlations,lncRNA-phenotype correlations,and the similar non-coding regions of the queried sequence in multiple *** sorted out a total of 203,391 lncRNA sequences,2000 phenotypes,and 120,271 variants of nine species(Zea mays L.,Gossypium barbadense L.,Triticum aestivum L.,Lycopersicon esculentum Mille,Oryza sativa L.,Hordeum vulgare L.,Sorghum bicolor L.,Glycine max L.,and Cucumis sativus L.).By exploring the relationship between lncRNAs and the genomic position of variants in genome-wide association analysis,a total of 68,862 lncRNAs were found to be related to the diversity of agronomic *** importantly,to facilitate the study of the functions of lncRNAs,we analyzed the possible target genes of lncRNAs,constructed a blast tool for performing similar fragmentation studies in all species,linked the pages of phenotypic studies related to lncRNAs that possess similar fragments and constructed their regulatory *** addition,LncPheDB also provides a user-friendly interface,a genome visualization platform,and multi-level and multi-modal convenient data search *** believe that LncPheDB plays a crucial role in mining lncRNA-related plant data.

关键词： LncRNA GWAS Phenotype SNP Plants

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温度对泥鳅胚胎卵裂间隔时间影响研究

河南师范大学学报（自然科学版） 2018年第4期46卷 74-80页

作者：刘自然潘淦华南师范大学生命科学学院广州510631 广州市金洋水产养殖有限公司广州511447

泥鳅是重要经济养殖鱼类,但是关于温度对泥鳅的受精卵卵裂研究还没有详细报道.本研究通过观察泥鳅的胚胎发育在不同水温(17±0.5)℃、(20±0.5)℃、(23±0.5)℃、(26±0.5)℃、(29±0.5)℃、(32±0.5)℃对卵裂间隔时间(T_0)的影响.结果... 详细信息

泥鳅是重要经济养殖鱼类,但是关于温度对泥鳅的受精卵卵裂研究还没有详细报道.本研究通过观察泥鳅的胚胎发育在不同水温(17±0.5)℃、(20±0.5)℃、(23±0.5)℃、(26±0.5)℃、(29±0.5)℃、(32±0.5)℃对卵裂间隔时间(T_0)的影响.结果表明:泥鳅胚胎发育可划分受精卵、卵裂期、原肠胚期、神经胚形成期、器官形成期、出膜期6个阶段.在一定水温范围内,第一次卵裂时间随水温升高而缩短,每升高1℃,约提前3.5min,通过回归分析第一次卵裂时间(y)和水温(x)的关系可以归纳为:y/min=-78.5ln(x/℃)+298.96(R^2=0.99).水温越高,卵裂间隔时间也越短,间隔时间与温度的回归方程为:y/min=44.188-0.002x/℃3+0.142 8x/℃2-3.553 6x/℃(R^2=0.987 1).

关键词：泥鳅胚胎发育水温卵裂间隔时间

Establishment of enzyme-linked immunosorbent assay for beef and lamb contents in cooked meat

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未来食品学报(英文) 2024年第1期4卷 91-96页

作者： Yujing Li Jingjing liu Sufang Fan Li Zhao Jing Zhang Erjing Zhang ziran Li Yan Zhang Chunsheng Li Hebei Jiaotong Vocational and Technical College Shijiazhuang 050035China Biology Institute of Hebei Academy of Sciences Shijiazhuang 050081China Hebei Food Safety Key Labratory Key Laboratory of Special Food Supenvision Technolog for State Market RegpulationHebei Engineering Research Center for Special Food Sagfery and HealthHebei Food Inspection cand Research InstinuteShijiazhuang 050227China College ofLife Sciences Hebei Normal UniversinyShijiazhuang 050024China Julu County Applied Technology Research Instinute Jultu 055250China

In this study,an enzyme 1linked immunosorbent assay(ELISA)was established to detect beef and 1amb components,and its performance was ***-antibody sandwich ELISA was adopted and determined a coating concentration of capture antibody 3G5 of 1:4000,a working concentration of enzyme-labeled antibody 2E7-horseradish peroxidase(HRP)of 1:1000,a sample incubation time of 60 min and a detection antibody reaction time of 60 *** specificity,sensitivity,repeatability and stability of this assay were *** limit of detection for beef and 1amb skeleta1 muscle troponin I was 45 mg/kg,the inter-assay and intra-assay recovery rates ranged from 80.4%to 115.7%,the coefficients of variation were below 13.6%,and the cIoss reaction rates of the tissue components of chicken,duck and fish were below 13.4%.The sandwich ELISA method established in this study is stable and has high *** test results were consistent with the polymerase chain reaction(PCR)method at 50 and 100 g/kg-Therefore,this ELISA method can be used to quantitatively detect beef and 1amb components in meat products.

关键词： Double antibody sandwich enzyme-1inked immunosorbent assay(ELISA) Beef components Lamb components

基于正交半监督局部Fisher判别分析的故障诊断

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机械工程学报 2014年第18期50卷 7-13页

作者：苏祖强汤宝平刘自然秦毅重庆大学机械传动国家重点实验室重庆400030 河南工业大学机电工程学院郑州450007

针对有标记故障样本不足和故障特征集维数过高的问题,提出基于正交半监督局部Fisher判别分析(Orthogonal semi-supervised local Fisher discriminant analysis,OSELF)的故障诊断方法。所提出的OSELF能够充分地利用蕴含于无标记故障样... 详细信息

针对有标记故障样本不足和故障特征集维数过高的问题,提出基于正交半监督局部Fisher判别分析(Orthogonal semi-supervised local Fisher discriminant analysis,OSELF)的故障诊断方法。所提出的OSELF能够充分地利用蕴含于无标记故障样本中的故障信息,避免了因有标记故障样本不足引起的过学习问题,同时采用正交迭代方式求解最优正交映射矩阵,克服现有方法无法得到正交映射矩阵的不足。正交映射矩阵的基矢量统计不相关,可有效地提高所得低维特征矢量的可辨识性。通过正交映射矩阵对故障样本集和新增样本进行维数约简,并将维数约简的结果输入粗糙优化k最近邻分类器(Coarse to fine k nearest neighbor classifier,CFKNNC)进行学习训练和故障识别。所提方法集成了OSELF在维数约简和CFKNNC在模式识别的优势,有效地提高了故障诊断的精度。通过齿轮箱故障模拟试验验证了该方法的有效性。

关键词：故障诊断维数约简正交半监督局部Fisher判别分析粗糙优化k最近邻分类器

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机械振动无线传感器网络跨层同步采集方法

仪器仪表学报 2014年第5期35卷 1143-1148页

作者：黄庆卿汤宝平邓蕾刘自然重庆大学机械传动国家重点实验室重庆400030 河南工业大学机电工程学院郑州450007

针对目前无线传感器网络性能偏低不能满足机械振动信号高精度同步采集需求的问题,提出了一种基于跨层设计的同步采集方法(SACD)。研究基于双核心设计的采集节点,提高任务调度实时性,降低采样间隔的抖动;实现帧起始定界符(SFD)的硬件跨... 详细信息

针对目前无线传感器网络性能偏低不能满足机械振动信号高精度同步采集需求的问题,提出了一种基于跨层设计的同步采集方法(SACD)。研究基于双核心设计的采集节点,提高任务调度实时性,降低采样间隔的抖动;实现帧起始定界符(SFD)的硬件跨层通信,避免命令传输过程中协议栈层与层、节点模块与模块之间的随机延时误差,提高时钟同步和同步触发精度;研究晶振频率偏移动态补偿方法,降低因采样间隔不相同导致的同步采集累积误差。实验结果表明,节点间最大同步触发误差的均值为1.107 2μs,最大值为1.28μs,连续采集10 s时的最大同步累积误差为7.54μs,能有效地提高机械振动无线传感网络同步采集精度。

关键词：机械振动监测无线传感器网络同步采集跨层设计

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非线性超声检测镁合金早期疲劳的试验研究

机械工程学报 2013年第4期49卷 20-24页

作者：颜丙生刘自然张跃春汤宝平河南工业大学机电工程学院郑州450007 中国航空动力机械研究所株洲412002

为利用基于二次谐波的非线性超声方法检测镁合金材料的早期疲劳损伤,建立超声非线性系数β的测试试验系统,设计制作预设应力集中区的厚板镁合金疲劳试件,并通过有限元法仿真应力集中区的有效性。对三个不同疲劳加载模式的镁合金试件进... 详细信息

为利用基于二次谐波的非线性超声方法检测镁合金材料的早期疲劳损伤,建立超声非线性系数β的测试试验系统,设计制作预设应力集中区的厚板镁合金疲劳试件,并通过有限元法仿真应力集中区的有效性。对三个不同疲劳加载模式的镁合金试件进行非线性超声检测试验研究。结果表明,在疲劳寿命的早期,三个试件的超声非线性系数均随疲劳周数明显增加。同时,测量其中一个试件在非应力集中区的超声非线性系数,与应力集中区相比,超声非线性系数变化幅度只有应力集中区的27%,进一步表明超声非线性系数的变化来源于镁合金材料疲劳引起的微缺陷变化,也证实试验的可靠性。另外,波速和弹性常数的试验结果表明,这两个线性参数对于镁合金材料的早期疲劳损伤不如超声非线性系数敏感。

关键词：非线性超声疲劳损伤 AZ31镁合金无损检测

Genome-wide selection and introgression of Chinese rice varieties during breeding

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Journal of Genetics and Genomics 2022年第5期49卷 492-501页

作者： Jinyue Ge Junrui Wang Hongbo Pang Fei Li Danjing Lou Weiya Fan ziran liu Jiaqi Li Danting Li Baoxuan Nong Zongqiong Zhang Yanyan Wang Jingfen Huang Meng Xing Yamin Nie Xiaorong Xiao Fan Zhang Wensheng Wang Jianlong Xu Sung Ryul Kim Ajay Kohli Guoyou Ye Weihua Qiao Qingwen Yang Xiaoming Zheng National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Guangxi Key Laboratory for Polysaccharide Materials and Modifications School of Marine Sciences and BiotechnologyGuangxi University for NationalitiesNanningJiangsu 530006China College of Life Science Shenyang Normal UniversityShenyangJilin 110034China Liaoning Institute of Pomology YingkouLiaoning 115009China Rice Research Institute Guangxi Academy of Agricultural ScienceNanningGuangxi 530007China National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanyaHainan 572000China International Rice Research Institute DAPO box 7777MetroManilaPhilippines Crop Biotech Institute&Department of Genetic Engineering Kyung Hee UniversityYongin 446-701Republic of Korea

China is the largest rice-producing country,but the genomic landscape of rice diversity has not yet been *** this study,we re-sequence 1070 rice varieties collected from China(400)and other regions in Asia(670).Among the six major rice groups(aus,indica-I,indica-II,aromatic,temperate japonica,and tropical japonica),almost all Chinese varieties belong to the indica-II or temperate japonica *** Chinese indica varieties belong to indica-II,which consists of two subgroups developed during different phases of rice *** genomic segments underlying the differences between these subgroups span36.32 *** Chinese japonica rice varieties fall into the temperate japonica group,consisting of two subgroups based on their geographical *** genomic segments underlying the differences between these subgroups span 27.69 *** differentiated segments in the Chinese indica varieties span 45 genes with nonsynonymous mutations that are closely related to variations in plant height and grain ***-four genes with nonsynonymous mutations are associated with the differences in heading date between the two Chinese japonica *** findings provide new insights into rice diversity in China that will facilitate the molecular breeding.

关键词： Rice Genome selection Introgression Agronomic traits Breeding